Reviewed, UniProtKB/Swiss-Prot P42910 (PTPC1_ECOLI)

Names and origin

Protein names

Recommended name:
    N-acetylgalactosamine permease IIC component 1
Alternative name(s):
    PTS system N-acetylgalactosamine-specific EIIC component 1
    EIIC-Aga

Gene names

Name:	agaC
Synonyms:	yraE
Ordered Locus Names:	b3139, JW3108

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIC type-4 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	N-acetylgalactosamine permease IIC component 1		PRO_0000186660
Regions
	Topological domain	1 – 10	10	Periplasmic Potential
	Transmembrane	11 – 31	21	Potential
	Topological domain	32 – 33	2	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Potential
	Topological domain	55 – 66	12	Periplasmic Potential
	Transmembrane	67 – 87	21	Potential
	Topological domain	88 – 94	7	Cytoplasmic Potential
	Transmembrane	95 – 115	21	Potential
	Topological domain	116 – 141	26	Periplasmic Potential
	Transmembrane	142 – 162	21	Potential
	Topological domain	163 – 177	15	Cytoplasmic Potential
	Transmembrane	178 – 198	21	Potential
	Topological domain	199 – 209	11	Periplasmic Potential
	Transmembrane	210 – 230	21	Potential
	Topological domain	231 – 267	37	Cytoplasmic Potential
	Domain	1 – 237	237	PTS EIIC type-4

Sequences

	Sequence		Length	Mass (Da)
	P42910-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C44F4D0827FE56C6	FASTA	267	28,645
	10 20 30 40 50 60 MHEITLLQGL SLAALVFVLG IDFWLEALFL FRPIIVCTLT GAILGDIQTG LITGGLTELA 70 80 90 100 110 120 FAGLTPAGGV QPPNPIMAGL MTTVIAWSTG VDAKTAIGLG LPFSLLMQYV ILFFYSAFSL 130 140 150 160 170 180 FMTKADKCAK EADTAAFSRL NWTTMLIVAS AYAVIAFLCT YLAQGAMQAL VKAMPAWLTH 190 200 210 220 230 240 GFEVAGGILP AVGFGLLLRV MFKAQYIPYL IAGFLFVCYI QVSNLLPVAV LGAGFAVYEF 250 260 FNAKSRQQAQ PQPVASKNEE EDYSNGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA57942.1. U00096 Genomic DNA. Translation: AAC76173.1. AP009048 Genomic DNA. Translation: BAE77185.1.
PIR	G65103.
RefSeq	AP_003684.1. NP_417608.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P42910.
Genome annotation databases
GeneID	947652.
GenomeReviews	Gene locus JW3108 in contig AP009048_GR. Gene locus b3139 in contig U00096_GR.
KEGG	ecj:JW3108. eco:b3139.
Organism-specific databases
EchoBASE	EB2623.
EcoGene	EG12770. agaC.
CMR	Search...
Phylogenomic databases
HOGENOM	P42910.
OMA	VESMPEW
Enzyme and pathway databases
BioCyc	EcoCyc:AGAC-MON. ECOL168927:B3139-MON.
Gene expression databases
Genevestigator	P42910.
Family and domain databases
InterPro	IPR004700. PTS_IIC_sorb. IPR018404. PTS_IIC_sorb_subgr. [Graphical view]
Pfam	PF03609. EII-Sor. 1 hit. [Graphical view]
TIGRFAMs	TIGR00822. EII-Sor. 1 hit.
PROSITE	PS51106. PTS_EIIC_TYPE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTPC1_ECOLI

Accession

Primary (citable) accession number: P42910
Secondary accession number(s): Q2M971

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)