Putative tagatose-6-phosphate ketose/aldose isomerase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P42907 (AGAS_ECOLI)

Last modified June 16, 2009. Version 52. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Putative tagatose-6-phosphate ketose/aldose isomerase
EC=5.3.1.-

Gene names

Name:	agaS
Synonyms:	yraB
Ordered Locus Names:	b3136, JW3105

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be a tagatose-6-phosphate ketose/aldose isomerase By similarity.
Miscellaneous	In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, agaS in K-12 strains is not involved in the degradation of these compounds.
Sequence similarities	Belongs to the SIS family. AgaS subfamily.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	intramolecular oxidoreductase activity, interconverting aldoses and ketoses Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

384

Putative tagatose-6-phosphate ketose/aldose isomerase

PRO_0000136590

Secondary structure

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384

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P42907-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 463F9458476DC46B
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384

41,783

        10         20         30         40         50         60 
MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR KENLRIILTG 

        70         80         90        100        110        120 
AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN PAHPLLLISF GRSGNSPESV 

       130        140        150        160        170        180 
AAVELANQFV PECYHLPITC NEAGALYQNA INSDNAFALL MPAETHDRGF AMTSSITTMM 

       190        200        210        220        230        240 
ASCLAVFAPE TINSQTFRDV ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR 

       250        260        270        280        290        300 
ESALKVLELT AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR 

       310        320        330        340        350        360 
RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA LMQSLHMGNT 

       370        380 
PDTPSASGTV NRVVQGVIIH PWQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[4]	"The crystal structure of the tagatose-6-phosphate ketose/aldose isomerase from Escherichia coli." Midwest center for structural genomics (MCSG) Submitted (FEB-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Cross-references

Sequence databases

U18997 Genomic DNA. Translation: AAA57939.1.
U00096 Genomic DNA. Translation: AAC76170.1.
AP009048 Genomic DNA. Translation: BAE77182.1.

PIR

RefSeq

AP_003681.1.
NP_417605.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C3J	X-ray	1.80	A/B/C/D/E/F	1-384	[»]

ModBase

Genome annotation databases

GeneID

GenomeReviews

Gene locus JW3105 in contig AP009048_GR.
Gene locus b3136 in contig U00096_GR.

KEGG

ecj:JW3105.
eco:b3136.

Organism-specific databases

EchoBASE

EcoGene

EG12767. agaS.

CMR

Phylogenomic databases

HOGENOM

OMA

P42907. SPLGFRH.

Enzyme and pathway databases

BioCyc

EcoCyc:G7634-MON.

Family and domain databases

InterPro

IPR001347. SIS.
IPR014180. Sugar_isomerase_AgaS.
[Graphical view]

Pfam

PF01380. SIS. 2 hits.
[Graphical view]

TIGRFAMs

TIGR02815. agaS_fam. 1 hit.

ProtoNet

Entry information

Entry name

AGAS_ECOLI

Accession

Primary (citable) accession number: P42907
Secondary accession number(s): Q2M974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents