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Protein

Putative D-galactosamine-6-phosphate deaminase AgaS

Gene

agaS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization-deamination of galactosamine 6-phosphate to form tagatofuranose 6-phosphate and ammonium ion.By similarity

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains cannot grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaS in K-12 strains is not involved in the degradation of these compounds.

Catalytic activityi

D-galactosamine 6-phosphate + H2O = D-tagatofuranose 6-phosphate + NH3.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G7634-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative D-galactosamine-6-phosphate deaminase AgaSCurated (EC:3.5.99.-By similarity)
Alternative name(s):
Gam-6-P deaminase/isomeraseBy similarity
Gene namesi
Name:agaS
Synonyms:yraB
Ordered Locus Names:b3136, JW3105
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12767 agaS

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001365901 – 384Putative D-galactosamine-6-phosphate deaminase AgaSAdd BLAST384

Proteomic databases

PaxDbiP42907
PRIDEiP42907

Interactioni

Protein-protein interaction databases

BioGridi4261158779 interactors.
IntActiP42907 5 interactors.
STRINGi316385.ECDH10B_3309

Structurei

Secondary structure

1384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 21Combined sources7
Helixi23 – 49Combined sources27
Beta strandi55 – 59Combined sources5
Helixi63 – 79Combined sources17
Beta strandi81 – 85Combined sources5
Helixi88 – 93Combined sources6
Helixi95 – 98Combined sources4
Beta strandi105 – 114Combined sources10
Helixi117 – 129Combined sources13
Beta strandi131 – 140Combined sources10
Helixi145 – 151Combined sources7
Helixi163 – 165Combined sources3
Beta strandi169 – 171Combined sources3
Helixi174 – 187Combined sources14
Turni189 – 191Combined sources3
Turni194 – 197Combined sources4
Helixi198 – 210Combined sources13
Turni211 – 213Combined sources3
Beta strandi225 – 232Combined sources8
Helixi235 – 249Combined sources15
Turni250 – 252Combined sources3
Beta strandi253 – 259Combined sources7
Helixi261 – 264Combined sources4
Turni265 – 267Combined sources3
Helixi268 – 271Combined sources4
Beta strandi276 – 281Combined sources6
Helixi288 – 302Combined sources15
Beta strandi305 – 314Combined sources10
Helixi317 – 320Combined sources4
Beta strandi322 – 326Combined sources5
Helixi335 – 356Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C3JX-ray1.80A/B/C/D/E/F1-384[»]
ProteinModelPortaliP42907
SMRiP42907
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42907

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 197SIS 1PROSITE-ProRule annotationAdd BLAST153
Domaini215 – 364SIS 2PROSITE-ProRule annotationAdd BLAST150

Sequence similaritiesi

Belongs to the SIS family. AgaS subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E70 Bacteria
COG2222 LUCA
HOGENOMiHOG000272306
InParanoidiP42907
KOiK02082
OMAiYHLIITC
PhylomeDBiP42907

Family and domain databases

CDDicd05010 SIS_AgaS_like, 1 hit
cd05008 SIS_GlmS_GlmD_1, 1 hit
InterProiView protein in InterPro
IPR035466 GlmS/AgaS_SIS
IPR001347 SIS
IPR035464 SIS_AgaS
IPR014180 Sugar_isomerase_AgaS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
TIGRFAMsiTIGR02815 agaS_fam, 1 hit
PROSITEiView protein in PROSITE
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

P42907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR
60 70 80 90 100
KENLRIILTG AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN
110 120 130 140 150
PAHPLLLISF GRSGNSPESV AAVELANQFV PECYHLPITC NEAGALYQNA
160 170 180 190 200
INSDNAFALL MPAETHDRGF AMTSSITTMM ASCLAVFAPE TINSQTFRDV
210 220 230 240 250
ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR ESALKVLELT
260 270 280 290 300
AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR
310 320 330 340 350
RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA
360 370 380
LMQSLHMGNT PDTPSASGTV NRVVQGVIIH PWQA
Length:384
Mass (Da):41,783
Last modified:November 1, 1995 - v1
Checksum:i463F9458476DC46B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57939.1
U00096 Genomic DNA Translation: AAC76170.1
AP009048 Genomic DNA Translation: BAE77182.1
PIRiD65103
RefSeqiNP_417605.1, NC_000913.3
WP_001114869.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76170; AAC76170; b3136
BAE77182; BAE77182; BAE77182
GeneIDi947645
KEGGiecj:JW3105
eco:b3136
PATRICifig|1411691.4.peg.3594

Similar proteinsi

Entry informationi

Entry nameiAGAS_ECOLI
AccessioniPrimary (citable) accession number: P42907
Secondary accession number(s): Q2M974
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome