Putative tagatose-6-phosphate ketose/aldose isomerase

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P42907 (AGAS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative tagatose-6-phosphate ketose/aldose isomerase
EC=5.3.1.-

Gene names

Name:	agaS
Synonyms:	yraB
Ordered Locus Names:	b3136, JW3105

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

May be a tagatose-6-phosphate ketose/aldose isomerase By similarity.

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaS in K-12 strains is not involved in the degradation of these compounds.

Sequence similarities

Belongs to the SIS family. AgaS subfamily.

Contains 2 SIS domains.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	intramolecular oxidoreductase activity, interconverting aldoses and ketoses Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 384

384

Putative tagatose-6-phosphate ketose/aldose isomerase

PRO_0000136590

Regions

Domain

45 – 197

153

SIS 1

Domain

215 – 364

150

SIS 2

Secondary structure

384

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P42907 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 463F9458476DC46B
Show »

FASTA

384

41,783

        10         20         30         40         50         60 
MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR KENLRIILTG 

        70         80         90        100        110        120 
AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN PAHPLLLISF GRSGNSPESV 

       130        140        150        160        170        180 
AAVELANQFV PECYHLPITC NEAGALYQNA INSDNAFALL MPAETHDRGF AMTSSITTMM 

       190        200        210        220        230        240 
ASCLAVFAPE TINSQTFRDV ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR 

       250        260        270        280        290        300 
ESALKVLELT AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR 

       310        320        330        340        350        360 
RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA LMQSLHMGNT 

       370        380 
PDTPSASGTV NRVVQGVIIH PWQA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[4]	"The crystal structure of the tagatose-6-phosphate ketose/aldose isomerase from Escherichia coli." Midwest center for structural genomics (MCSG) Submitted (FEB-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18997 Genomic DNA. Translation: AAA57939.1.
U00096 Genomic DNA. Translation: AAC76170.1.
AP009048 Genomic DNA. Translation: BAE77182.1.

PIR

D65103.

RefSeq

NP_417605.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C3J	X-ray	1.80	A/B/C/D/E/F	1-384	[»]

ProteinModelPortal

P42907.

SMR

P42907. Positions 4-384.

ModBase

Search...

Protein-protein interaction databases

IntAct

P42907. 5 interactions.

Proteomic databases

PRIDE

P42907.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004869; EBESCP00000004869; EBESCG00000003974.
EBESCT00000017660; EBESCP00000016951; EBESCG00000016716.

GeneID

947645.

GenomeReviews

Gene locus JW3105 in contig AP009048_GR.
Gene locus b3136 in contig U00096_GR.

KEGG

ecj:JW3105.
eco:b3136.

PATRIC

32121690. VBIEscCol129921_3231.

Organism-specific databases

EchoBASE

EB2620.

EcoGene

EG12767. agaS.

Phylogenomic databases

eggNOG

COG2222.

GeneTree

EBGT00050000009350.

HOGENOM

HBG342689.

OMA

MTGSFSC.

PhylomeDB

P42907.

ProtClustDB

CLSK877085.

Enzyme and pathway databases

BioCyc

EcoCyc:G7634-MONOMER.

Gene expression databases

Genevestigator

P42907.

Family and domain databases

InterPro

IPR001347. SIS.
IPR014180. Sugar_isomerase_AgaS.
[Graphical view]

K02082.

Pfam

PF01380. SIS. 2 hits.
[Graphical view]

TIGRFAMs

TIGR02815. AgaS_fam. 1 hit.

PROSITE

PS51464. SIS. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AGAS_ECOLI

Accession

Primary (citable) accession number: P42907
Secondary accession number(s): Q2M974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents