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P42907 (AGAS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Putative tagatose-6-phosphate ketose/aldose isomerase

EC=5.3.1.-
Gene names
Name:agaS
Synonyms:yraB
Ordered Locus Names:b3136, JW3105
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be a tagatose-6-phosphate ketose/aldose isomerase By similarity.

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaS in K-12 strains is not involved in the degradation of these compounds.

Sequence similarities

Belongs to the SIS family. AgaS subfamily.

Contains 2 SIS domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Putative tagatose-6-phosphate ketose/aldose isomerase
PRO_0000136590

Regions

Domain45 – 197153SIS 1
Domain215 – 364150SIS 2

Secondary structure

......................................................... 384
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42907 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 463F9458476DC46B

FASTA38441,783
        10         20         30         40         50         60 
MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR KENLRIILTG 

        70         80         90        100        110        120 
AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN PAHPLLLISF GRSGNSPESV 

       130        140        150        160        170        180 
AAVELANQFV PECYHLPITC NEAGALYQNA INSDNAFALL MPAETHDRGF AMTSSITTMM 

       190        200        210        220        230        240 
ASCLAVFAPE TINSQTFRDV ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR 

       250        260        270        280        290        300 
ESALKVLELT AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR 

       310        320        330        340        350        360 
RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA LMQSLHMGNT 

       370        380 
PDTPSASGTV NRVVQGVIIH PWQA 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli."
Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr.
Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.
[4]"The crystal structure of the tagatose-6-phosphate ketose/aldose isomerase from Escherichia coli."
Midwest center for structural genomics (MCSG)
Submitted (FEB-2008) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18997 Genomic DNA. Translation: AAA57939.1.
U00096 Genomic DNA. Translation: AAC76170.1.
AP009048 Genomic DNA. Translation: BAE77182.1.
PIRD65103.
RefSeqNP_417605.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3C3JX-ray1.80A/B/C/D/E/F1-384[»]
ProteinModelPortalP42907.
SMRP42907. Positions 4-384.
ModBaseSearch...

Protein-protein interaction databases

IntActP42907. 5 interactions.

Proteomic databases

PRIDEP42907.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000004869; EBESCP00000004869; EBESCG00000003974.
EBESCT00000017660; EBESCP00000016951; EBESCG00000016716.
GeneID947645.
GenomeReviewsGene locus JW3105 in contig AP009048_GR.
Gene locus b3136 in contig U00096_GR.
KEGGecj:JW3105.
eco:b3136.
PATRIC32121690. VBIEscCol129921_3231.

Organism-specific databases

EchoBASEEB2620.
EcoGeneEG12767. agaS.

Phylogenomic databases

eggNOGCOG2222.
GeneTreeEBGT00050000009350.
HOGENOMHBG342689.
OMAMTGSFSC.
PhylomeDBP42907.
ProtClustDBCLSK877085.

Enzyme and pathway databases

BioCycEcoCyc:G7634-MONOMER.

Gene expression databases

GenevestigatorP42907.

Family and domain databases

InterProIPR001347. SIS.
IPR014180. Sugar_isomerase_AgaS.
[Graphical view]
KOK02082.
PfamPF01380. SIS. 2 hits.
[Graphical view]
TIGRFAMsTIGR02815. AgaS_fam. 1 hit.
PROSITEPS51464. SIS. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGAS_ECOLI
AccessionPrimary (citable) accession number: P42907
Secondary accession number(s): Q2M974
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families