Reviewed, UniProtKB/Swiss-Prot P42906 (AGAA_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative N-acetylgalactosamine-6-phosphate deacetylase
    EC=3.5.1.-

Gene names

Name:	agaA
Ordered Locus Names:	b3135, JW5527

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Catalytic activity	N-acetyl-D-galactosamine 6-phosphate + H2O = D-galactosamine 6-phosphate + acetate.
Miscellaneous	In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, agaA in K-12 strains is not involved in the degradation of these compounds.
Sequence similarities	Belongs to the nagA family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AAA57938.1 differs from that shown. Reason: Frameshift at position 114.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Putative N-acetylgalactosamine-6-phosphate deacetylase		PRO_0000170920
Experimental info
	Sequence conflict	145	1	S → R in AAA57938. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42906-1 [UniParc]. Last modified October 11, 2004. Version 3. Checksum: 1C3CB5AC3168FEEA	FASTA	167	17,519
	10 20 30 40 50 60 MHCYNGMTGL HHREPGMVGA GLTDKRAWLE LIADGHHVHP AAMSLCCCCA KERIVLITDA 70 80 90 100 110 120 MQAAGMPDGR YTLCGEEVQM HGGVVRTASG GLAGSTLSVD AAVRNMVELT GVTPAEAIHM 130 140 150 160 ASLHPARMLG VDGVLGSLKP GKRASVVALD SGLHVQQIWI QGQLASF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 145.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA57938.1. Frameshift. U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. Translation: BAE77181.1.
PIR	C65103.
RefSeq	AP_003680.1.
3D structure databases
HSSP	HSSP built from PDB template 1O12 based on UniProtKB Q9WZS1.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus JW5527 in contig AP009048_GR. Gene locus b3135 in contig U00096_GR.
KEGG	ecj:JW5527. eco:b3135.
Organism-specific databases
EchoBASE	EB2619.
EcoGene	EG12766. agaA.
CMR	Search...
Phylogenomic databases
HOGENOM	P42906.
OMA	P42906. VAPEEND.
Enzyme and pathway databases
BioCyc	EcoCyc:AGAA-MON.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

AGAA_ECOLI

Accession

Primary (citable) accession number: P42906
Secondary accession number(s): P76670, Q2M975, Q6BF43

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)