Reviewed, UniProtKB/Swiss-Prot P42905 (PTPC2_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative N-acetylgalactosamine permease IIC component 2
Alternative name(s):
    PTS system N-acetylgalactosamine-specific EIIC component 2
    EIIC-Aga'

Gene names

Name:	agaW
Ordered Locus Names:	b3134

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Note: When overexpressed.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIC type-4 domain.
Caution	Could be the product of a pseudogene.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	Putative N-acetylgalactosamine permease IIC component 2		PRO_0000186661
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Potential
	Topological domain	24 – 32	9	Periplasmic Potential
	Transmembrane	33 – 53	21	Potential
	Topological domain	54 – 65	12	Cytoplasmic Potential
	Transmembrane	66 – 86	21	Potential
	Topological domain	87 – 93	7	Periplasmic Potential
	Transmembrane	94 – 114	21	Potential
	Topological domain	115 – 133	19	Cytoplasmic Potential
	Domain	1 – 133	133	PTS EIIC type-4

Sequences

	Sequence		Length	Mass (Da)
	P42905-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6F45186ABE8D7C38	FASTA	133	13,721
	10 20 30 40 50 60 MEISLLQAFA LGIIAFIAGL DMFNGLTHMH RPVVLGPLVG LVLGDLHTGI LTGGTLELVW 70 80 90 100 110 120 MGLAPLAGAQ PPNVIIGTIV GTAFAITTGV KPDVAVGVAV PFAVAVQMGI TFLFSVMSGV 130 MSRCDLATNP RRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[3]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA57937.1. U00096 Genomic DNA. No translation available.
PIR	B65103.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus b3134 in contig U00096_GR.
KEGG	eco:b3134.
Organism-specific databases
EchoBASE	EB2618.
EcoGene	EG12765. agaW.
CMR	Search...
Phylogenomic databases
HOGENOM	P42905.
Enzyme and pathway databases
BioCyc	EcoCyc:G7633-MON.
Family and domain databases
InterPro	IPR004700. PTS_IIC_sorb. [Graphical view]
Pfam	PF03609. EII-Sor. 1 hit. [Graphical view]
PROSITE	PS51106. PTS_EIIC_TYPE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTPC2_ECOLI

Accession

Primary (citable) accession number: P42905

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)