P42904 (PTPB2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 2
EC=2.7.1.69

Alternative name(s):
EIIB-Aga'
PTS system N-acetylgalactosamine-specific EIIB component 2

Gene names

Name:	agaV
Synonyms:	yhaY
Ordered Locus Names:	b3133, JW3102

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-4 domain.
Sequence caution	The sequence AAA57936.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 2		PRO_0000186659
Regions
	Domain	1 – 157	157	PTS EIIB type-4
Sites
	Active site	15	1	Pros-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42904 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3DC895AD0CF15D41	FASTA	157	17,086
	10 20 30 40 50 60 MPNIVLSRID ERLIHGQVGV QWVGFAGANL VLVANDEVAE DPVQQNLMEM VLAEGIAVRF 70 80 90 100 110 120 WTLQKVIDNI HRAADRQKIL LVCKTPADFL TLVKGGVPVN RINVGNMHYA NGKQQIAKTV 130 140 150 SVDAGDIAAF NDLKTAGVEC FVQGVPTEPA VDLFKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57936.1. Different initiation. U00096 Genomic DNA. Translation: AAC76167.2. AP009048 Genomic DNA. Translation: BAE77180.1.
RefSeq	NP_417602.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P42904.
SMR	P42904. Positions 3-153.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42904. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004141; EBESCP00000004141; EBESCG00000003380. EBESCT00000015110; EBESCP00000014401; EBESCG00000014170.
GeneID	947648.
GenomeReviews	Gene locus JW3102 in contig AP009048_GR. Gene locus b3133 in contig U00096_GR.
KEGG	ecj:JW3102. eco:b3133.
PATRIC	32121682. VBIEscCol129921_3227.
Organism-specific databases
EchoBASE	EB2617.
EcoGene	EG12764. agaV.
Phylogenomic databases
eggNOG	COG3444.
GeneTree	EBGT00050000009724.
HOGENOM	HBG702768.
OMA	RVPDEAG.
PhylomeDB	P42904.
ProtClustDB	PRK11425.
Enzyme and pathway databases
BioCyc	EcoCyc:G7632-MONOMER.
Gene expression databases
Genevestigator	P42904.
Family and domain databases
InterPro	IPR004720. PTS_IIB_sorbose-sp. IPR018455. PTS_IIB_sorbose-sp_subgr. [Graphical view]
Gene3D	G3DSA:3.40.35.10. PTS_IIB_sorb. 1 hit.
KO	K02745.
Pfam	PF03830. PTSIIB_sorb. 1 hit. [Graphical view]
SUPFAM	SSF52728. PTS_IIB_sorb. 1 hit.
TIGRFAMs	TIGR00854. Pts-sorbose. 1 hit.
PROSITE	PS51101. PTS_EIIB_TYPE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTPB2_ECOLI

Accession

Primary (citable) accession number: P42904
Secondary accession number(s): P76669, Q2M976

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program