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Protein

N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 2

Gene

agaV

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport.

Catalytic activityi

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei15 – 151Pros-phosphohistidine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7632-MONOMER.
ECOL316407:JW3102-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 2 (EC:2.7.1.-)
Alternative name(s):
EIIB-Aga'
PTS system N-acetylgalactosamine-specific EIIB component 2
Gene namesi
Name:agaV
Synonyms:yhaY
Ordered Locus Names:b3133, JW3102
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12764. agaV.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 2PRO_0000186659Add
BLAST

Proteomic databases

PaxDbiP42904.
PRIDEiP42904.

Interactioni

Protein-protein interaction databases

BioGridi4261156. 10 interactions.
IntActiP42904. 2 interactions.
STRINGi511145.b3133.

Structurei

3D structure databases

ProteinModelPortaliP42904.
SMRiP42904. Positions 2-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 157157PTS EIIB type-4PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-4 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108RG5. Bacteria.
COG3444. LUCA.
HOGENOMiHOG000096437.
InParanoidiP42904.
KOiK02745.
OMAiLVCKSPA.
OrthoDBiEOG6FJNGT.
PhylomeDBiP42904.

Family and domain databases

Gene3Di3.40.35.10. 1 hit.
InterProiIPR004720. PTS_IIB_sorbose-sp.
IPR018455. PTS_IIB_sorbose-sp_subgr.
[Graphical view]
PfamiPF03830. PTSIIB_sorb. 1 hit.
[Graphical view]
SUPFAMiSSF52728. SSF52728. 1 hit.
TIGRFAMsiTIGR00854. pts-sorbose. 1 hit.
PROSITEiPS51101. PTS_EIIB_TYPE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNIVLSRID ERLIHGQVGV QWVGFAGANL VLVANDEVAE DPVQQNLMEM
60 70 80 90 100
VLAEGIAVRF WTLQKVIDNI HRAADRQKIL LVCKTPADFL TLVKGGVPVN
110 120 130 140 150
RINVGNMHYA NGKQQIAKTV SVDAGDIAAF NDLKTAGVEC FVQGVPTEPA

VDLFKLL
Length:157
Mass (Da):17,086
Last modified:November 1, 1995 - v1
Checksum:i3DC895AD0CF15D41
GO

Sequence cautioni

The sequence AAA57936.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57936.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76167.2.
AP009048 Genomic DNA. Translation: BAE77180.1.
RefSeqiNP_417602.4. NC_000913.3.
WP_001336162.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76167; AAC76167; b3133.
BAE77180; BAE77180; BAE77180.
GeneIDi947648.
KEGGiecj:JW3102.
eco:b3133.
PATRICi32121682. VBIEscCol129921_3227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57936.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76167.2.
AP009048 Genomic DNA. Translation: BAE77180.1.
RefSeqiNP_417602.4. NC_000913.3.
WP_001336162.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP42904.
SMRiP42904. Positions 2-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261156. 10 interactions.
IntActiP42904. 2 interactions.
STRINGi511145.b3133.

Proteomic databases

PaxDbiP42904.
PRIDEiP42904.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76167; AAC76167; b3133.
BAE77180; BAE77180; BAE77180.
GeneIDi947648.
KEGGiecj:JW3102.
eco:b3133.
PATRICi32121682. VBIEscCol129921_3227.

Organism-specific databases

EchoBASEiEB2617.
EcoGeneiEG12764. agaV.

Phylogenomic databases

eggNOGiENOG4108RG5. Bacteria.
COG3444. LUCA.
HOGENOMiHOG000096437.
InParanoidiP42904.
KOiK02745.
OMAiLVCKSPA.
OrthoDBiEOG6FJNGT.
PhylomeDBiP42904.

Enzyme and pathway databases

BioCyciEcoCyc:G7632-MONOMER.
ECOL316407:JW3102-MONOMER.

Miscellaneous databases

PROiP42904.

Family and domain databases

Gene3Di3.40.35.10. 1 hit.
InterProiIPR004720. PTS_IIB_sorbose-sp.
IPR018455. PTS_IIB_sorbose-sp_subgr.
[Graphical view]
PfamiPF03830. PTSIIB_sorb. 1 hit.
[Graphical view]
SUPFAMiSSF52728. SSF52728. 1 hit.
TIGRFAMsiTIGR00854. pts-sorbose. 1 hit.
PROSITEiPS51101. PTS_EIIB_TYPE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli."
    Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr.
    Microbiology 142:231-250(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiPTPB2_ECOLI
AccessioniPrimary (citable) accession number: P42904
Secondary accession number(s): P76669, Q2M976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.