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Protein

Enolase

Gene
N/A
Organism
Alligator mississippiensis (American alligator)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Y1Q_0005850), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Y1Q_0005850)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase, Alpha-enolase
  5. Pyruvate kinase (PKM), Pyruvate kinase (Y1Q_0000374)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136SubstrateBy similarity1
Binding sitei145SubstrateBy similarity1
Active sitei188Proton donorBy similarity1
Metal bindingi223MagnesiumBy similarity1
Metal bindingi271MagnesiumBy similarity1
Binding sitei271SubstrateBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Active sitei321Proton acceptorBy similarity1
Binding sitei372SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
OrganismiAlligator mississippiensis (American alligator)
Taxonomic identifieri8496 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaCrocodyliaAlligatoridaeAlligatorinaeAlligator

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000134095‹1 – ›395EnolaseAdd BLAST›395

Proteomic databases

PRIDEiP42897.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP42897.
SMRiP42897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni348 – 351Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOVERGENiHBG000067.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Fragment.

P42897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DLYTSKGLFR AAVPSGASTG IYEALELRDN DKTRFMGKGV SKAVAHVNKT
60 70 80 90 100
IAPALISKNI SVVEQEKIDR LMLEMDGSEN KSKFGANAIL GVSLAVCKAG
110 120 130 140 150
AAEKGVPLYR HIADLAGNPE VILPVPAFNV INGGSHAGNK LAMQEFMILP
160 170 180 190 200
VGAESFKEAM RIGAEVYHNL KNVIKEKYGK DATNVGDEGG FAPNILENKE
210 220 230 240 250
ALELLKNAIN KAGYSDKIVI GMDVAASEFY RDGKYDLDFK SPDDPSRYIT
260 270 280 290 300
PDQLADLYKS FVKNYPVVSI EDPFDQDDWA AWKKFTASVG IQVVGDDLTV
310 320 330 340 350
TNPKRIAKAV DDKACNCLLL KVNQIGSVTE SLQACKLAQS NGWGVMVSHR
360 370 380 390
SGETEDTFIA DLVVGLCTGQ IKTGAPCRSE RLAKYNQILR IEEEL
Length:395
Mass (Da):42,884
Last modified:November 1, 1995 - v1
Checksum:iB43E91228E9110B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei3951

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28078 mRNA. Translation: AAA53671.1.
PIRiI50026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28078 mRNA. Translation: AAA53671.1.
PIRiI50026.

3D structure databases

ProteinModelPortaliP42897.
SMRiP42897.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP42897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000067.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_ALLMI
AccessioniPrimary (citable) accession number: P42897
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 15, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.