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Protein

Enolase

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. Enolase
  5. Pyruvate kinase isozyme G, chloroplastic, Pyruvate kinase isozyme A, chloroplastic, Pyruvate kinase (RCOM_0910580), Pyruvate kinase (RCOM_1122030), Pyruvate kinase (RCOM_0840740), Pyruvate kinase (RCOM_0865230), Pyruvate kinase (RCOM_0342830), Pyruvate kinase (RCOM_1581420), Pyruvate kinase (RCOM_0248610), Pyruvate kinase (RCOM_1382790), Pyruvate kinase (RCOM_1454580), Pyruvate kinase (RCOM_1382780)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Metal bindingi251MagnesiumBy similarity1
Metal bindingi301MagnesiumBy similarity1
Binding sitei301SubstrateBy similarity1
Metal bindingi328MagnesiumBy similarity1
Binding sitei328SubstrateBy similarity1
Active sitei353Proton acceptorBy similarity1
Binding sitei404SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

SABIO-RKP42896.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340781 – 445EnolaseAdd BLAST445

Proteomic databases

PRIDEiP42896.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP42896.
SMRiP42896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 383Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

KOiK01689.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P42896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAITIVSVRA RQIFDSRGNP TVEADIKLSD GHLARAAVPS GASTGIYEAL
60 70 80 90 100
ELRDGGSDYL GKGVSKAVEN VNSIIGPALI GKDPTEQTAL DNFMVQELDG
110 120 130 140 150
TVNEWGWCKQ KLGANAILAV SLALCKAGAH VKGIPLYKHI ANLAGNKNLV
160 170 180 190 200
LPVPAFNVIN GGSHAGNKLA MQEFMILPVG ASSFKEAMKM GAEVYHHLKS
210 220 230 240 250
VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIAKA GYTGKVVIGM
260 270 280 290 300
DVAASEFYGS DKTYDLNFKE ENNDGSQKIS GEALKDLYKS FASEYPIVSI
310 320 330 340 350
EDPFDQDDWE HYSKLTSEIG EKVQIVGDDL LVTNPKRVEK AIQEKACNAL
360 370 380 390 400
LLKVNQIGSV TESIEAVRMS KRAGWGVMAS HRSGETEDTF IADLSVGLAT
410 420 430 440
GQIKTGAPCR SERLAKYNQL LRIEEELGAE AVYAGAKFRT PVEPY
Length:445
Mass (Da):47,912
Last modified:November 1, 1995 - v1
Checksum:iA88EFE3A90CAE572
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28386 mRNA. Translation: CAA82232.1.
PIRiS39203.
RefSeqiNP_001310701.1. NM_001323772.1.

Genome annotation databases

GeneIDi8288376.
KEGGircu:8288376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28386 mRNA. Translation: CAA82232.1.
PIRiS39203.
RefSeqiNP_001310701.1. NM_001323772.1.

3D structure databases

ProteinModelPortaliP42896.
SMRiP42896.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP42896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8288376.
KEGGircu:8288376.

Phylogenomic databases

KOiK01689.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
SABIO-RKP42896.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_RICCO
AccessioniPrimary (citable) accession number: P42896
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 15, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.