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Protein

Endothelin-converting enzyme 1

Gene

ECE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts big endothelin-1 to endothelin-1.

Catalytic activityi

Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphoramidon.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi591 – 5911Zinc; catalyticPROSITE-ProRule annotation
Active sitei592 – 5921PROSITE-ProRule annotation
Metal bindingi595 – 5951Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi651 – 6511Zinc; catalyticPROSITE-ProRule annotation
Active sitei655 – 6551Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  • endopeptidase activity Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  • peptide hormone processing Source: BHF-UCL
  • protein processing Source: BHF-UCL
  • proteolysis Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP42891.

Protein family/group databases

MEROPSiM13.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-converting enzyme 1 (EC:3.4.24.71)
Short name:
ECE-1
Gene namesi
Name:ECE1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence analysisAdd
BLAST
Transmembranei53 – 7321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini74 – 754681ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: BHF-UCL
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2340.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Endothelin-converting enzyme 1PRO_0000078218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphothreonineBy similarity
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence analysis
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence analysis
Glycosylationi346 – 3461N-linked (GlcNAc...)Sequence analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence analysis
Disulfide bondi412 – 412InterchainBy similarity
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence analysis
Glycosylationi616 – 6161N-linked (GlcNAc...)Sequence analysis
Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP42891.
PRIDEiP42891.

Expressioni

Gene expression databases

ExpressionAtlasiP42891. baseline and differential.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055669.

Chemistry

BindingDBiP42891.

Structurei

3D structure databases

ProteinModelPortaliP42891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOVERGENiHBG005554.
InParanoidiP42891.
OrthoDBiEOG7PZRWQ.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR029734. ECE1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF130. PTHR11733:SF130. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTYKRATL DEEDLVDSLS ESDVYPNHLQ VNFRGPRNGQ RCWAARTPVE
60 70 80 90 100
KRLVVLVALL AAALVACLAV LGIQYQTRTP SVCLSEACIS VTSSILSSMD
110 120 130 140 150
PTVDPCQDFF TYACGGWIKA NPVPDGHSRW GTFSNLWEHN QAIIKHLLEN
160 170 180 190 200
STASVSEAER KAQVYYRACM NETRIEELKA KPLMELIEKL GGWNITGPWD
210 220 230 240 250
KDNFQDTLQV VTSHYHTSPF FSVYVSADSK NSNSNVIQVD QSGLGLPSRD
260 270 280 290 300
YYLNKTENEK VLTGYLNYMV QLGKLLGGGA EDTIRPQMQQ ILDFETALAN
310 320 330 340 350
ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNTIF YPVEINESEP
360 370 380 390 400
IVIYDKEYLS KVSTLINSTD KCLLNNYMIW NLVRKTSSFL DQRFQDADEK
410 420 430 440 450
FMEVMYGTKK TCLPRWKFCV SDTENTLGFA LGPMFVKATF AEDSKNIASE
460 470 480 490 500
IILEIKKAFE ESLSTLKWMD EDTRKSAKEK ADAIYNMIGY PNFIMDPKEL
510 520 530 540 550
DKVFNDYTAV PDLYFENAMR FFNFSWRVTA DQLRKAPNRD QWSMTPPMVN
560 570 580 590 600
AYYSPTKNEI VFPAGILQAP FYTRSSPNAL NFGGIGVVVG HELTHAFDDQ
610 620 630 640 650
GREYDKDGNL RPWWKNSSVE AFKQQTACMV EQYGNYSVNG EPVNGRHTLG
660 670 680 690 700
ENIADNGGLK AAYRAYQNWV KKNGAEQTLP TLGLTNNQLF FLSFAQVWCS
710 720 730 740 750
VRTPESSHEG LITDPHSPSR FRVIGSISNS KEFSEHFHCP PGSPMNPHHK

CEVW
Length:754
Mass (Da):85,517
Last modified:February 26, 2008 - v2
Checksum:i763D8E2664E3A540
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81A → P in CAA84547 (PubMed:7805846).Curated
Sequence conflicti120 – 1201A → S in AAI33321 (Ref. 2) Curated
Sequence conflicti162 – 1643AQV → DQE in CAA84547 (PubMed:7805846).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35306 mRNA. Translation: CAA84547.1.
BC133320 mRNA. Translation: AAI33321.1.
PIRiS51010. S47268.
RefSeqiXP_005203129.1. XM_005203072.3.
UniGeneiBt.4378.

Genome annotation databases

EnsembliENSBTAT00000037770; ENSBTAP00000037593; ENSBTAG00000002977.
GeneIDi281133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35306 mRNA. Translation: CAA84547.1.
BC133320 mRNA. Translation: AAI33321.1.
PIRiS51010. S47268.
RefSeqiXP_005203129.1. XM_005203072.3.
UniGeneiBt.4378.

3D structure databases

ProteinModelPortaliP42891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055669.

Chemistry

BindingDBiP42891.
ChEMBLiCHEMBL2340.

Protein family/group databases

MEROPSiM13.002.

Proteomic databases

PaxDbiP42891.
PRIDEiP42891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000037770; ENSBTAP00000037593; ENSBTAG00000002977.
GeneIDi281133.

Organism-specific databases

CTDi1889.

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOVERGENiHBG005554.
InParanoidiP42891.
OrthoDBiEOG7PZRWQ.

Enzyme and pathway databases

SABIO-RKP42891.

Miscellaneous databases

PROiP42891.

Gene expression databases

ExpressionAtlasiP42891. baseline and differential.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR029734. ECE1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF130. PTHR11733:SF130. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of human and bovine endothelin converting enzyme (ECE-1)."
    Schmidt M., Kroeger B., Jacob E., Seulberger H., Subkowski T., Otter R., Meyer T., Schmalzing G., Hillen H.
    FEBS Lett. 356:238-243(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiECE1_BOVIN
AccessioniPrimary (citable) accession number: P42891
Secondary accession number(s): A2VDM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 26, 2008
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.