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Protein

Endothelin-converting enzyme 1

Gene

ECE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts big endothelin-1 to endothelin-1.

Catalytic activityi

Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphoramidon.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi591Zinc; catalyticPROSITE-ProRule annotation1
Active sitei592PROSITE-ProRule annotation1
Metal bindingi595Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi651Zinc; catalyticPROSITE-ProRule annotation1
Active sitei655Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

  • endopeptidase activity Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  • peptide hormone processing Source: BHF-UCL
  • protein processing Source: BHF-UCL
  • proteolysis Source: AgBase

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-375276 Peptide ligand-binding receptors
SABIO-RKP42891

Protein family/group databases

MEROPSiM13.002

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-converting enzyme 1 (EC:3.4.24.71)
Short name:
ECE-1
Gene namesi
Name:ECE1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Organism-specific databases

VGNCiVGNC:28302 ECE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52CytoplasmicSequence analysisAdd BLAST52
Transmembranei53 – 73Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini74 – 754ExtracellularSequence analysisAdd BLAST681

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2340

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000782181 – 754Endothelin-converting enzyme 1Add BLAST754

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphothreonineBy similarity1
Glycosylationi150N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi171N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi300N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi412InterchainBy similarity
Glycosylationi523N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi616N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi635N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP42891
PeptideAtlasiP42891
PRIDEiP42891

Expressioni

Gene expression databases

BgeeiENSBTAG00000002977
ExpressionAtlasiP42891 baseline and differential

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Interacts with PPP1R16B (PubMed:26806547).By similarity1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000055669

Chemistry databases

BindingDBiP42891

Structurei

3D structure databases

ProteinModelPortaliP42891
SMRiP42891
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624 Eukaryota
COG3590 LUCA
GeneTreeiENSGT00760000119162
HOVERGENiHBG005554
InParanoidiP42891

Family and domain databases

CDDicd08662 M13, 1 hit
Gene3Di3.40.390.10, 3 hits
InterProiView protein in InterPro
IPR029733 ECE1/ECE2
IPR024079 MetalloPept_cat_dom_sf
IPR000718 Peptidase_M13
IPR018497 Peptidase_M13_C
IPR008753 Peptidase_M13_N
PANTHERiPTHR11733 PTHR11733, 1 hit
PTHR11733:SF130 PTHR11733:SF130, 1 hit
PfamiView protein in Pfam
PF01431 Peptidase_M13, 1 hit
PF05649 Peptidase_M13_N, 1 hit
PRINTSiPR00786 NEPRILYSIN
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

P42891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTYKRATL DEEDLVDSLS ESDVYPNHLQ VNFRGPRNGQ RCWAARTPVE
60 70 80 90 100
KRLVVLVALL AAALVACLAV LGIQYQTRTP SVCLSEACIS VTSSILSSMD
110 120 130 140 150
PTVDPCQDFF TYACGGWIKA NPVPDGHSRW GTFSNLWEHN QAIIKHLLEN
160 170 180 190 200
STASVSEAER KAQVYYRACM NETRIEELKA KPLMELIEKL GGWNITGPWD
210 220 230 240 250
KDNFQDTLQV VTSHYHTSPF FSVYVSADSK NSNSNVIQVD QSGLGLPSRD
260 270 280 290 300
YYLNKTENEK VLTGYLNYMV QLGKLLGGGA EDTIRPQMQQ ILDFETALAN
310 320 330 340 350
ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNTIF YPVEINESEP
360 370 380 390 400
IVIYDKEYLS KVSTLINSTD KCLLNNYMIW NLVRKTSSFL DQRFQDADEK
410 420 430 440 450
FMEVMYGTKK TCLPRWKFCV SDTENTLGFA LGPMFVKATF AEDSKNIASE
460 470 480 490 500
IILEIKKAFE ESLSTLKWMD EDTRKSAKEK ADAIYNMIGY PNFIMDPKEL
510 520 530 540 550
DKVFNDYTAV PDLYFENAMR FFNFSWRVTA DQLRKAPNRD QWSMTPPMVN
560 570 580 590 600
AYYSPTKNEI VFPAGILQAP FYTRSSPNAL NFGGIGVVVG HELTHAFDDQ
610 620 630 640 650
GREYDKDGNL RPWWKNSSVE AFKQQTACMV EQYGNYSVNG EPVNGRHTLG
660 670 680 690 700
ENIADNGGLK AAYRAYQNWV KKNGAEQTLP TLGLTNNQLF FLSFAQVWCS
710 720 730 740 750
VRTPESSHEG LITDPHSPSR FRVIGSISNS KEFSEHFHCP PGSPMNPHHK

CEVW
Length:754
Mass (Da):85,517
Last modified:February 26, 2008 - v2
Checksum:i763D8E2664E3A540
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8A → P in CAA84547 (PubMed:7805846).Curated1
Sequence conflicti120A → S in AAI33321 (Ref. 2) Curated1
Sequence conflicti162 – 164AQV → DQE in CAA84547 (PubMed:7805846).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35306 mRNA Translation: CAA84547.1
BC133320 mRNA Translation: AAI33321.1
PIRiS51010 S47268
RefSeqiXP_005203129.1, XM_005203072.3
UniGeneiBt.4378

Genome annotation databases

EnsembliENSBTAT00000037770; ENSBTAP00000037593; ENSBTAG00000002977
GeneIDi281133

Similar proteinsi

Entry informationi

Entry nameiECE1_BOVIN
AccessioniPrimary (citable) accession number: P42891
Secondary accession number(s): A2VDM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 26, 2008
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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