P42888 (URED_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreD

Gene names

Name:	ureD
Ordered Locus Names:	R02476
ORF Names:	SMc01942

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01384
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01384.
Sequence similarities	Belongs to the UreD family.
Sequence caution	The sequence AAB33024.2 differs from that shown. Reason: Frameshift at position 39.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Urease accessory protein UreD HAMAP MF_01384		PRO_0000067614
Experimental info
	Sequence conflict	37	1	C → S Ref.1
	Sequence conflict	97 – 103	7	AEIATRI → PRLRRRV in AAB33024. Ref.1
	Sequence conflict	171	1	G → A in AAB33024. Ref.1
	Sequence conflict	171	1	G → A in AAB30133. Ref.1
	Sequence conflict	181	1	A → S in AAB33024. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42888 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: F800A62163D7C9C0	FASTA	277	29,366
	10 20 30 40 50 60 MEPAAIIAPQ RARGEGRLVA KAEGGRTRIA ELYQEGCAKI RLPKTFDASM EAVLINSSGG 70 80 90 100 110 120 VTGGDRLSWE FRAGKGTKLT LTTQACEKVY KAAAGTAEIA TRISVAAGAH VDWLPQETIL 130 140 150 160 170 180 FDRSALSRSL EVDLAADASF LAVEAVLIGR KAMGEEVRAG LFRDNWRIRS GGRLIHAENL 190 200 210 220 230 240 ALAGDIAALA SRRAVLDGAA AFATLVYAAP DCESQLSKLR LALAGHALSG VSHYDVGGRD 250 260 270 KIVARVAAAD GFALRKILIP LISHLRKDAS VPKVWTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The urease structural gene ureA in Rhizobium meliloti is preceded by an open reading frame necessary for urease activity." Miksch G. FEMS Microbiol. Lett. 124:185-190(1994) [PubMed: 7813887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AK631.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[4]	"A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureA, ureB, ureC) interrupted by other open reading frames." Miksch G., Arnold W., Lentzsch P., Priefer U.B., Puehler A. Mol. Gen. Genet. 242:539-550(1994) [PubMed: 8121412] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-277. Strain: AK631.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S76260 Genomic DNA. Translation: AAB33024.2. Frameshift. AL591688 Genomic DNA. Translation: CAC47055.1. S69145 Genomic DNA. Translation: AAB30133.1.
PIR	S42601.
RefSeq	NP_386582.1. NC_003047.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1234144.
GenomeReviews	Gene locus R02476 in contig AL591688_GR.
KEGG	sme:SMc01942.
NMPDR	fig|266834.1.peg.3770.
PATRIC	23634455. VBISinMel96828_3965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG711156.
OMA	QETILFD.
ProtClustDB	CLSK2484748.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01942-MONOMER.
Family and domain databases
HAMAP	MF_01384. UreD. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
KO	K03190.
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED_RHIME

Accession

Primary (citable) accession number: P42888

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program