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Protein

Urease subunit alpha

Gene

ureC

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit gamma (ureA), Urease subunit alpha (ureC)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi138Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi219Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi219Nickel 2; via carbamate groupUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Metal bindingi248Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi274Nickel 2; via tele nitrogenUniRule annotation1
Active sitei322Proton donorUniRule annotation1
Metal bindingi362Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11554.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:R02470
ORF Names:SMc01837
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000675511 – 570Urease subunit alphaAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc01837.

Structurei

3D structure databases

ProteinModelPortaliP42885.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 570UreaseUniRule annotationAdd BLAST440

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYRMSRAAY ANMFGPTVGD KVRLADTELF IEVEKDFTTH GEEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ VTREGGAVDT VITNALILDH WGIVKADIGL KDGRIAAIGK
110 120 130 140 150
AGNPDMQPGV TIIVGPGTEV IAGEGKIVTA GGMDSHIHFI CPQQIEEALM
160 170 180 190 200
SGLTCMLGGG TGPAHGTLAT TCTPGPWHIA RMIEAADAFP MNLAFAGKGN
210 220 230 240 250
ASLPGALVEM VLGGATSLKL HEDWGTTPAA IDCCLSVADE YDVQVMIHTD
260 270 280 290 300
TLNESGFVED TIAAIKGRTI HAYHTEGAGG GHAPDIIRIC GQPNVIPSST
310 320 330 340 350
NPTRPYTVNT LAEHLDMLMV CHHLSPTIPE DIAFAESRIR KETIAAEDIL
360 370 380 390 400
HDIGAFSIIS SDSQAMGRVG EVAIRTWQTA DKMKRQRGRL KEETGDNDNF
410 420 430 440 450
RVKRYIAKYT INPAIAHGLS HEIGSLEVGK RADLVLWNPA FFGVKPDMVL
460 470 480 490 500
LGGTIAAAPM GDPNASIPTP QPVHYRPMFG AYGRSRTNSS VTFVSQASLD
510 520 530 540 550
AGLAGRLGVA KELVAVQNTR GGIGKASMIH NSLTPHIEVD PETYEVRADG
560 570
ELLTCEPATV LPMAQRYFLF
Length:570
Mass (Da):60,769
Last modified:May 30, 2000 - v2
Checksum:i431A547F6221DCA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti496Q → P in AAB30138 (PubMed:8121412).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69145 Genomic DNA. Translation: AAB30138.1.
AL591688 Genomic DNA. Translation: CAC47049.1.
PIRiS42607.
RefSeqiNP_386576.1. NC_003047.1.
WP_010969961.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC47049; CAC47049; SMc01837.
GeneIDi1234138.
KEGGisme:SMc01837.
PATRICi23634443. VBISinMel96828_3959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69145 Genomic DNA. Translation: AAB30138.1.
AL591688 Genomic DNA. Translation: CAC47049.1.
PIRiS42607.
RefSeqiNP_386576.1. NC_003047.1.
WP_010969961.1. NC_003047.1.

3D structure databases

ProteinModelPortaliP42885.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc01837.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC47049; CAC47049; SMc01837.
GeneIDi1234138.
KEGGisme:SMc01837.
PATRICi23634443. VBISinMel96828_3959.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciMetaCyc:MONOMER-11554.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_RHIME
AccessioniPrimary (citable) accession number: P42885
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.