P42883 (THI12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrimidine precursor biosynthesis enzyme THI12

Gene names

Name:	THI12
Ordered Locus Names:	YNL332W
ORF Names:	N0295

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety. Ref.4
Sequence similarities	Belongs to the NMT1 family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Pyrimidine precursor biosynthesis enzyme THI12		PRO_0000211620

Sequences

	Sequence		Length	Mass (Da)
	P42883 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F7C23A05AB5CA5F0	FASTA	340	38,620
	10 20 30 40 50 60 MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE LIGSGKVDMG 70 80 90 100 110 120 LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT EDFQSLKGKK IGYVGEFGKI 130 140 150 160 170 180 QIDELTKHYG MKPEDYTAVR CGMNVAKYII EGKIDAGIGI ECMQQVELEE YLAKQGRPAS 190 200 210 220 230 240 DAKMLRIDKL ACLGCCCFCT VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK 250 260 270 280 290 300 EYIDFKPRLN NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 310 320 330 340 YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase." van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A. Yeast 11:987-991(1995) [PubMed: 8533474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine." Wightman R., Meacock P.A. Microbiology 149:1447-1460(2003) [PubMed: 12777485] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83226 Genomic DNA. Translation: CAA58226.1. Z71608 Genomic DNA. Translation: CAA96265.1. BK006947 Genomic DNA. Translation: DAA10231.1.
RefSeq	NP_014067.1. NM_001183170.1.
3D structure databases
ProteinModelPortal	P42883.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42883. 1 interaction.
STRING	P42883.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL332W; YNL332W; YNL332W.
GeneID	855384.
KEGG	sce:YNL332W.
Organism-specific databases
CYGD	YNL332w.
SGD	S000005276. THI12.
Phylogenomic databases
eggNOG	fuNOG06211.
GeneTree	EFGT00050000005197.
HOGENOM	HBG330298.
OMA	ESEHIAD.
OrthoDB	EOG4SN4XF.
Gene expression databases
Genevestigator	P42883.
GermOnline	YNL332W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015168. NMT1. [Graphical view]
Pfam	PF09084. NMT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979181.

Entry information

Entry name

THI12_YEAST

Accession

Primary (citable) accession number: P42883
Secondary accession number(s): D6W0L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program