Reviewed, UniProtKB/Swiss-Prot P42883 (THI12_YEAST)

Names and origin

Protein names

Recommended name:
    Pyrimidine precursor biosynthesis enzyme THI12

Gene names

Name:	THI12
Ordered Locus Names:	YNL332W
ORF Names:	N0295

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety. Ref.3
Sequence similarities	Belongs to the NMT1 family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Pyrimidine precursor biosynthesis enzyme THI12		PRO_0000211620

Sequences

	Sequence		Length	Mass (Da)
	P42883-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F7C23A05AB5CA5F0	FASTA	340	38,620
	10 20 30 40 50 60 MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE LIGSGKVDMG 70 80 90 100 110 120 LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT EDFQSLKGKK IGYVGEFGKI 130 140 150 160 170 180 QIDELTKHYG MKPEDYTAVR CGMNVAKYII EGKIDAGIGI ECMQQVELEE YLAKQGRPAS 190 200 210 220 230 240 DAKMLRIDKL ACLGCCCFCT VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK 250 260 270 280 290 300 EYIDFKPRLN NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 310 320 330 340 YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase." van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A. Yeast 11:987-991(1995) [PubMed: 8533474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine." Wightman R., Meacock P.A. Microbiology 149:1447-1460(2003) [PubMed: 12777485] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83226 Genomic DNA. Translation: CAA58226.1. Z71608 Genomic DNA. Translation: CAA96265.1.
RefSeq	NP_014067.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P42883. 1 interaction.
Genome annotation databases
Ensembl	YNL332W; YNL332W; YNL332W; Saccharomyces cerevisiae. [Genome view]
GeneID	855384.
KEGG	sce:YNL332W.
Organism-specific databases
CYGD	YNL332w.
SGD	S000005276. THI12.
Phylogenomic databases
HOGENOM	P42883.
OrthoDB	EOG9K3NCD
Gene expression databases
Genevestigator	P42883.
GermOnline	YNL332W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015168. NMT1. [Graphical view]
Pfam	PF09084. NMT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979181.

Entry information

Entry name

THI12_YEAST

Accession

Primary (citable) accession number: P42883

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)