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P42881

- GPT_SCHPO

UniProt

P42881 - GPT_SCHPO

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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

gpt2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Inhibited by tunicamycin.

Pathwayi

GO - Molecular functioni

  1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: PomBase

GO - Biological processi

  1. dolichyl diphosphate biosynthetic process Source: PomBase
  2. N-glycan processing Source: PomBase
  3. protein N-linked glycosylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_236966. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:gpt2
Synonyms:gpt
ORF Names:SPBC15D4.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC15D4.04.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence AnalysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei155 – 17521HelicalSequence AnalysisAdd
BLAST
Transmembranei191 – 21121HelicalSequence AnalysisAdd
BLAST
Transmembranei216 – 23621HelicalSequence AnalysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence AnalysisAdd
BLAST
Transmembranei282 – 30221HelicalSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. integral component of endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108764Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP42881.

Interactioni

Protein-protein interaction databases

MINTiMINT-4690327.
STRINGi4896.SPBC15D4.04-1.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0472.
HOGENOMiHOG000163915.
InParanoidiP42881.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG7380DT.
PhylomeDBiP42881.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIESCFNVGI WATGLALLMN QGQSPLLSNV GLSVLAYKAT AMFIPRVGPS
60 70 80 90 100
FIKRGFSGKD MNKVEKYVIP ETMGAVSALV YFMCMIIFIP VLFYKYLVPN
110 120 130 140 150
HNPNLPSDGS VAEVAKSQFP HDLLGAYLSA LLSILSVSLL GILDDLFDIR
160 170 180 190 200
WRHKFFLPAI AAIPLLVVYY VDYGVTYVSV PSIVRPFLKR SLINLGFLYY
210 220 230 240 250
FYMAAVAIFC PNSINIIAGV NGVEAGQSLV LALVIACNDL FYVLSPKNKD
260 270 280 290 300
ALRAHLLSLY LVLPLIGVTA GLLKYNWWPS RVFVGDTFCY FAGMVMAVVG
310 320 330 340 350
ILGHFSKTLM LFFIPQIFNF ALSVPQLFGL VECPRHRLPK LNVKTGLLEN
360 370 380 390 400
SYTEFSLNEH PLPKKTLLTI SIFEKLRLIR VEYDPSTGRP LRCTNFTIIN
410 420 430 440
FVLYHLGPMR EDHLTICIMG LQLLTGIFGL IIRHFVAPLV YPEDNI
Length:446
Mass (Da):49,855
Last modified:November 1, 1995 - v1
Checksum:i16CAB90E5FBFF15F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09454 Genomic DNA. Translation: AAA92799.1.
CU329671 Genomic DNA. Translation: CAA20479.1.
PIRiS71622.
RefSeqiNP_596244.1. NM_001022163.2.

Genome annotation databases

EnsemblFungiiSPBC15D4.04.1; SPBC15D4.04.1:pep; SPBC15D4.04.
GeneIDi2539795.
KEGGispo:SPBC15D4.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09454 Genomic DNA. Translation: AAA92799.1 .
CU329671 Genomic DNA. Translation: CAA20479.1 .
PIRi S71622.
RefSeqi NP_596244.1. NM_001022163.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4690327.
STRINGi 4896.SPBC15D4.04-1.

Proteomic databases

MaxQBi P42881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC15D4.04.1 ; SPBC15D4.04.1:pep ; SPBC15D4.04 .
GeneIDi 2539795.
KEGGi spo:SPBC15D4.04.

Organism-specific databases

PomBasei SPBC15D4.04.

Phylogenomic databases

eggNOGi COG0472.
HOGENOMi HOG000163915.
InParanoidi P42881.
KOi K01001.
OMAi IHERNLT.
OrthoDBi EOG7380DT.
PhylomeDBi P42881.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_236966. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi 20800944.
PROi P42881.

Family and domain databases

InterProi IPR000715. Glycosyl_transferase_4.
[Graphical view ]
Pfami PF00953. Glycos_transf_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Asparagine-linked glycosylation in Schizosaccharomyces pombe: functional conservation of the first step in oligosaccharide-lipid assembly."
    Zou J., Scocca J.R., Krag S.S.
    Arch. Biochem. Biophys. 317:487-496(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiGPT_SCHPO
AccessioniPrimary (citable) accession number: P42881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3