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P42881

- GPT_SCHPO

UniProt

P42881 - GPT_SCHPO

Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

gpt2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

    Enzyme regulationi

    Inhibited by tunicamycin.

    Pathwayi

    GO - Molecular functioni

    1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: PomBase

    GO - Biological processi

    1. dolichyl diphosphate biosynthetic process Source: PomBase
    2. N-glycan processing Source: PomBase
    3. protein N-linked glycosylation Source: PomBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
    Alternative name(s):
    GlcNAc-1-P transferase
    Short name:
    G1PT
    Short name:
    GPT
    N-acetylglucosamine-1-phosphate transferase
    Gene namesi
    Name:gpt2
    Synonyms:gpt
    ORF Names:SPBC15D4.04
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC15D4.04.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. integral component of endoplasmic reticulum membrane Source: PomBase
    3. integral component of membrane Source: PomBase

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108764Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP42881.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4690327.
    STRINGi4896.SPBC15D4.04-1.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei25 – 4521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei73 – 9321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei155 – 17521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei191 – 21121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei216 – 23621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei282 – 30221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0472.
    HOGENOMiHOG000163915.
    KOiK01001.
    OMAiIHERNLT.
    OrthoDBiEOG7380DT.
    PhylomeDBiP42881.

    Family and domain databases

    InterProiIPR000715. Glycosyl_transferase_4.
    [Graphical view]
    PfamiPF00953. Glycos_transf_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42881-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIESCFNVGI WATGLALLMN QGQSPLLSNV GLSVLAYKAT AMFIPRVGPS    50
    FIKRGFSGKD MNKVEKYVIP ETMGAVSALV YFMCMIIFIP VLFYKYLVPN 100
    HNPNLPSDGS VAEVAKSQFP HDLLGAYLSA LLSILSVSLL GILDDLFDIR 150
    WRHKFFLPAI AAIPLLVVYY VDYGVTYVSV PSIVRPFLKR SLINLGFLYY 200
    FYMAAVAIFC PNSINIIAGV NGVEAGQSLV LALVIACNDL FYVLSPKNKD 250
    ALRAHLLSLY LVLPLIGVTA GLLKYNWWPS RVFVGDTFCY FAGMVMAVVG 300
    ILGHFSKTLM LFFIPQIFNF ALSVPQLFGL VECPRHRLPK LNVKTGLLEN 350
    SYTEFSLNEH PLPKKTLLTI SIFEKLRLIR VEYDPSTGRP LRCTNFTIIN 400
    FVLYHLGPMR EDHLTICIMG LQLLTGIFGL IIRHFVAPLV YPEDNI 446
    Length:446
    Mass (Da):49,855
    Last modified:November 1, 1995 - v1
    Checksum:i16CAB90E5FBFF15F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09454 Genomic DNA. Translation: AAA92799.1.
    CU329671 Genomic DNA. Translation: CAA20479.1.
    PIRiS71622.
    RefSeqiNP_596244.1. NM_001022163.2.

    Genome annotation databases

    EnsemblFungiiSPBC15D4.04.1; SPBC15D4.04.1:pep; SPBC15D4.04.
    GeneIDi2539795.
    KEGGispo:SPBC15D4.04.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09454 Genomic DNA. Translation: AAA92799.1 .
    CU329671 Genomic DNA. Translation: CAA20479.1 .
    PIRi S71622.
    RefSeqi NP_596244.1. NM_001022163.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4690327.
    STRINGi 4896.SPBC15D4.04-1.

    Proteomic databases

    MaxQBi P42881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC15D4.04.1 ; SPBC15D4.04.1:pep ; SPBC15D4.04 .
    GeneIDi 2539795.
    KEGGi spo:SPBC15D4.04.

    Organism-specific databases

    PomBasei SPBC15D4.04.

    Phylogenomic databases

    eggNOGi COG0472.
    HOGENOMi HOG000163915.
    KOi K01001.
    OMAi IHERNLT.
    OrthoDBi EOG7380DT.
    PhylomeDBi P42881.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 20800944.
    PROi P42881.

    Family and domain databases

    InterProi IPR000715. Glycosyl_transferase_4.
    [Graphical view ]
    Pfami PF00953. Glycos_transf_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Asparagine-linked glycosylation in Schizosaccharomyces pombe: functional conservation of the first step in oligosaccharide-lipid assembly."
      Zou J., Scocca J.R., Krag S.S.
      Arch. Biochem. Biophys. 317:487-496(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiGPT_SCHPO
    AccessioniPrimary (citable) accession number: P42881
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3