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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

gpt2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Inhibited by tunicamycin.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol metabolic process Source: GO_Central
  • dolichyl diphosphate biosynthetic process Source: PomBase
  • N-glycan processing Source: PomBase
  • protein N-linked glycosylation Source: PomBase
  • UDP-N-acetylglucosamine metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:gpt2
Synonyms:gpt
ORF Names:SPBC15D4.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.04.
PomBaseiSPBC15D4.04. gpt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Transmembranei191 – 211HelicalSequence analysisAdd BLAST21
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Transmembranei254 – 274HelicalSequence analysisAdd BLAST21
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087641 – 446UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP42881.
PRIDEiP42881.

Interactioni

Protein-protein interaction databases

BioGridi276345. 1 interactor.
MINTiMINT-4690327.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000163915.
InParanoidiP42881.
KOiK01001.
OMAiWAFPELP.
OrthoDBiEOG092C2SKV.
PhylomeDBiP42881.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIESCFNVGI WATGLALLMN QGQSPLLSNV GLSVLAYKAT AMFIPRVGPS
60 70 80 90 100
FIKRGFSGKD MNKVEKYVIP ETMGAVSALV YFMCMIIFIP VLFYKYLVPN
110 120 130 140 150
HNPNLPSDGS VAEVAKSQFP HDLLGAYLSA LLSILSVSLL GILDDLFDIR
160 170 180 190 200
WRHKFFLPAI AAIPLLVVYY VDYGVTYVSV PSIVRPFLKR SLINLGFLYY
210 220 230 240 250
FYMAAVAIFC PNSINIIAGV NGVEAGQSLV LALVIACNDL FYVLSPKNKD
260 270 280 290 300
ALRAHLLSLY LVLPLIGVTA GLLKYNWWPS RVFVGDTFCY FAGMVMAVVG
310 320 330 340 350
ILGHFSKTLM LFFIPQIFNF ALSVPQLFGL VECPRHRLPK LNVKTGLLEN
360 370 380 390 400
SYTEFSLNEH PLPKKTLLTI SIFEKLRLIR VEYDPSTGRP LRCTNFTIIN
410 420 430 440
FVLYHLGPMR EDHLTICIMG LQLLTGIFGL IIRHFVAPLV YPEDNI
Length:446
Mass (Da):49,855
Last modified:November 1, 1995 - v1
Checksum:i16CAB90E5FBFF15F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09454 Genomic DNA. Translation: AAA92799.1.
CU329671 Genomic DNA. Translation: CAA20479.1.
PIRiS71622.
RefSeqiNP_596244.1. NM_001022163.2.

Genome annotation databases

EnsemblFungiiSPBC15D4.04.1; SPBC15D4.04.1:pep; SPBC15D4.04.
GeneIDi2539795.
KEGGispo:SPBC15D4.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09454 Genomic DNA. Translation: AAA92799.1.
CU329671 Genomic DNA. Translation: CAA20479.1.
PIRiS71622.
RefSeqiNP_596244.1. NM_001022163.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276345. 1 interactor.
MINTiMINT-4690327.

Proteomic databases

MaxQBiP42881.
PRIDEiP42881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC15D4.04.1; SPBC15D4.04.1:pep; SPBC15D4.04.
GeneIDi2539795.
KEGGispo:SPBC15D4.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.04.
PomBaseiSPBC15D4.04. gpt2.

Phylogenomic databases

HOGENOMiHOG000163915.
InParanoidiP42881.
KOiK01001.
OMAiWAFPELP.
OrthoDBiEOG092C2SKV.
PhylomeDBiP42881.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP42881.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPT_SCHPO
AccessioniPrimary (citable) accession number: P42881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.