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P42867

- GPT_MOUSE

UniProt

P42867 - GPT_MOUSE

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Protein
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Gene
Dpagt1, Dpagt2, Gnpta
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi

GO - Molecular functioni

  1. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: MGI
  2. UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity Source: MGI
  3. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  4. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. UDP-N-acetylglucosamine metabolic process Source: Ensembl
  2. dolichol biosynthetic process Source: Ensembl
  3. dolichol-linked oligosaccharide biosynthetic process Source: MGI
  4. protein N-linked glycosylation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:Dpagt1
Synonyms:Dpagt2, Gnpta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1196396. Dpagt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Lumenal Reviewed prediction
Transmembranei7 – 3428Helical; Reviewed prediction
Add
BLAST
Topological domaini35 – 5925Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei60 – 8122Helical; Reviewed prediction
Add
BLAST
Topological domaini82 – 9615Lumenal Reviewed prediction
Add
BLAST
Transmembranei97 – 11620Helical; Reviewed prediction
Add
BLAST
Topological domaini117 – 12711Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei128 – 14720Helical; Reviewed prediction
Add
BLAST
Topological domaini148 – 16619Lumenal Reviewed prediction
Add
BLAST
Transmembranei167 – 18620Helical; Reviewed prediction
Add
BLAST
Topological domaini187 – 19610Cytoplasmic Reviewed prediction
Transmembranei197 – 21317Helical; Reviewed prediction
Add
BLAST
Topological domaini214 – 22310Lumenal Reviewed prediction
Transmembranei224 – 24219Helical; Reviewed prediction
Add
BLAST
Topological domaini243 – 25412Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei255 – 27117Helical; Reviewed prediction
Add
BLAST
Topological domaini272 – 2765Lumenal Reviewed prediction
Transmembranei277 – 29620Helical; Reviewed prediction
Add
BLAST
Topological domaini297 – 38084Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei381 – 39919Helical; Reviewed prediction
Add
BLAST
Topological domaini400 – 41011Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die 4 to 5 days post-fertilization, just after implantation, suggesting that protein function and N-glycosylation are essential in early embryogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
PRO_0000108762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi148 – 1481N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP42867.
PRIDEiP42867.

PTM databases

PhosphoSiteiP42867.

Expressioni

Developmental stagei

Highest activity is during the mid-phase of lactation.

Gene expression databases

ArrayExpressiP42867.
BgeeiP42867.
GenevestigatoriP42867.

Structurei

3D structure databases

ProteinModelPortaliP42867.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 8113Dolichol recognition
Add
BLAST
Motifi224 – 23613Dolichol recognition
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiP42867.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG73804W.
PhylomeDBiP42867.
TreeFamiTF313734.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42867-1 [UniParc]FASTAAdd to Basket

« Hide

MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK    50
LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL 100
IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT 150
IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS 200
LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV 250
FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP 300
CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG 350
ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR 400
YQLVRLFYDV 410
Length:410
Mass (Da):46,412
Last modified:November 28, 2003 - v2
Checksum:iF729D6983FA67E0B
GO

RNA editingi

Partially edited.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti74 – 741C → Y in RNA edited version.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65603 mRNA. Translation: CAA46553.1.
BC010474 mRNA. Translation: AAH10474.1.
CCDSiCCDS23104.1.
PIRiS24326.
RefSeqiNP_031901.2. NM_007875.2.
UniGeneiMm.18353.

Genome annotation databases

EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123.
GeneIDi13478.
KEGGimmu:13478.
UCSCiuc009pcv.1. mouse.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65603 mRNA. Translation: CAA46553.1 .
BC010474 mRNA. Translation: AAH10474.1 .
CCDSi CCDS23104.1.
PIRi S24326.
RefSeqi NP_031901.2. NM_007875.2.
UniGenei Mm.18353.

3D structure databases

ProteinModelPortali P42867.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P42867.

Proteomic databases

PaxDbi P42867.
PRIDEi P42867.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000054708 ; ENSMUSP00000056282 ; ENSMUSG00000032123 .
GeneIDi 13478.
KEGGi mmu:13478.
UCSCi uc009pcv.1. mouse.

Organism-specific databases

CTDi 1798.
MGIi MGI:1196396. Dpagt1.

Phylogenomic databases

eggNOGi COG0472.
GeneTreei ENSGT00390000011424.
HOGENOMi HOG000163915.
HOVERGENi HBG000846.
InParanoidi P42867.
KOi K01001.
OMAi IHERNLT.
OrthoDBi EOG73804W.
PhylomeDBi P42867.
TreeFami TF313734.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

ChiTaRSi DPAGT1. mouse.
NextBioi 283963.
PROi P42867.
SOURCEi Search...

Gene expression databases

ArrayExpressi P42867.
Bgeei P42867.
Genevestigatori P42867.

Family and domain databases

InterProi IPR000715. Glycosyl_transferase_4.
[Graphical view ]
Pfami PF00953. Glycos_transf_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal localization."
    Rajput B., Ma J., Muniappa N., Schantz L., Naylor S.L., Lalley P.A., Vijay I.K.
    Biochem. J. 285:985-992(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  2. "Structure and organization of mouse GlcNAc-1-phosphate transferase gene."
    Rajput B., Ma J., Vijay I.K.
    J. Biol. Chem. 269:9590-9597(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING.
    Strain: BALB/c and NIH Swiss.
    Tissue: Liver and Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. "A recessive deletion in the GlcNAc-1-phosphotransferase gene results in peri-implantation embryonic lethality."
    Marek K.W., Vijay I.K., Marth J.D.
    Glycobiology 9:1263-1271(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGPT_MOUSE
AccessioniPrimary (citable) accession number: P42867
Secondary accession number(s): Q921W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 28, 2003
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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