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P42867

- GPT_MOUSE

UniProt

P42867 - GPT_MOUSE

Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

Dpagt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (28 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

    Enzyme regulationi

    Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

    Pathwayi

    GO - Molecular functioni

    1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: MGI
    4. UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity Source: MGI

    GO - Biological processi

    1. dolichol biosynthetic process Source: Ensembl
    2. dolichol-linked oligosaccharide biosynthetic process Source: MGI
    3. protein N-linked glycosylation Source: Ensembl
    4. UDP-N-acetylglucosamine metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
    Alternative name(s):
    GlcNAc-1-P transferase
    Short name:
    G1PT
    Short name:
    GPT
    N-acetylglucosamine-1-phosphate transferase
    Gene namesi
    Name:Dpagt1
    Synonyms:Dpagt2, Gnpta
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1196396. Dpagt1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice die 4 to 5 days post-fertilization, just after implantation, suggesting that protein function and N-glycosylation are essential in early embryogenesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108762Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP42867.
    PRIDEiP42867.

    PTM databases

    PhosphoSiteiP42867.

    Expressioni

    Developmental stagei

    Highest activity is during the mid-phase of lactation.

    Gene expression databases

    ArrayExpressiP42867.
    BgeeiP42867.
    GenevestigatoriP42867.

    Structurei

    3D structure databases

    ProteinModelPortaliP42867.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66LumenalSequence Analysis
    Topological domaini35 – 5925CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 9615LumenalSequence AnalysisAdd
    BLAST
    Topological domaini117 – 12711CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini148 – 16619LumenalSequence AnalysisAdd
    BLAST
    Topological domaini187 – 19610CytoplasmicSequence Analysis
    Topological domaini214 – 22310LumenalSequence Analysis
    Topological domaini243 – 25412CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini272 – 2765LumenalSequence Analysis
    Topological domaini297 – 38084CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini400 – 41011LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 3428HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8122HelicalSequence AnalysisAdd
    BLAST
    Transmembranei97 – 11620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei128 – 14720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei197 – 21317HelicalSequence AnalysisAdd
    BLAST
    Transmembranei224 – 24219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27117HelicalSequence AnalysisAdd
    BLAST
    Transmembranei277 – 29620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei381 – 39919HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 8113Dolichol recognitionAdd
    BLAST
    Motifi224 – 23613Dolichol recognitionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0472.
    GeneTreeiENSGT00390000011424.
    HOGENOMiHOG000163915.
    HOVERGENiHBG000846.
    InParanoidiP42867.
    KOiK01001.
    OMAiIHERNLT.
    OrthoDBiEOG73804W.
    PhylomeDBiP42867.
    TreeFamiTF313734.

    Family and domain databases

    InterProiIPR000715. Glycosyl_transferase_4.
    [Graphical view]
    PfamiPF00953. Glycos_transf_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42867-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK    50
    LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL 100
    IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT 150
    IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS 200
    LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV 250
    FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP 300
    CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG 350
    ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR 400
    YQLVRLFYDV 410
    Length:410
    Mass (Da):46,412
    Last modified:November 28, 2003 - v2
    Checksum:iF729D6983FA67E0B
    GO

    RNA editingi

    Partially edited.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti74 – 741C → Y in RNA edited version.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65603 mRNA. Translation: CAA46553.1.
    BC010474 mRNA. Translation: AAH10474.1.
    CCDSiCCDS23104.1.
    PIRiS24326.
    RefSeqiNP_031901.2. NM_007875.2.
    UniGeneiMm.18353.

    Genome annotation databases

    EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123.
    GeneIDi13478.
    KEGGimmu:13478.
    UCSCiuc009pcv.1. mouse.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65603 mRNA. Translation: CAA46553.1 .
    BC010474 mRNA. Translation: AAH10474.1 .
    CCDSi CCDS23104.1.
    PIRi S24326.
    RefSeqi NP_031901.2. NM_007875.2.
    UniGenei Mm.18353.

    3D structure databases

    ProteinModelPortali P42867.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P42867.

    Proteomic databases

    PaxDbi P42867.
    PRIDEi P42867.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054708 ; ENSMUSP00000056282 ; ENSMUSG00000032123 .
    GeneIDi 13478.
    KEGGi mmu:13478.
    UCSCi uc009pcv.1. mouse.

    Organism-specific databases

    CTDi 1798.
    MGIi MGI:1196396. Dpagt1.

    Phylogenomic databases

    eggNOGi COG0472.
    GeneTreei ENSGT00390000011424.
    HOGENOMi HOG000163915.
    HOVERGENi HBG000846.
    InParanoidi P42867.
    KOi K01001.
    OMAi IHERNLT.
    OrthoDBi EOG73804W.
    PhylomeDBi P42867.
    TreeFami TF313734.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    ChiTaRSi DPAGT1. mouse.
    NextBioi 283963.
    PROi P42867.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P42867.
    Bgeei P42867.
    Genevestigatori P42867.

    Family and domain databases

    InterProi IPR000715. Glycosyl_transferase_4.
    [Graphical view ]
    Pfami PF00953. Glycos_transf_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal localization."
      Rajput B., Ma J., Muniappa N., Schantz L., Naylor S.L., Lalley P.A., Vijay I.K.
      Biochem. J. 285:985-992(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Mammary gland.
    2. "Structure and organization of mouse GlcNAc-1-phosphate transferase gene."
      Rajput B., Ma J., Vijay I.K.
      J. Biol. Chem. 269:9590-9597(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING.
      Strain: BALB/c and NIH Swiss.
      Tissue: Liver and Mammary gland.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    4. "A recessive deletion in the GlcNAc-1-phosphotransferase gene results in peri-implantation embryonic lethality."
      Marek K.W., Vijay I.K., Marth J.D.
      Glycobiology 9:1263-1271(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiGPT_MOUSE
    AccessioniPrimary (citable) accession number: P42867
    Secondary accession number(s): Q921W5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 28, 2003
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3