Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

Dpagt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:Dpagt1
Synonyms:Dpagt2, Gnpta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1196396 Dpagt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 34HelicalSequence analysisAdd BLAST28
Topological domaini35 – 59CytoplasmicSequence analysisAdd BLAST25
Transmembranei60 – 81HelicalSequence analysisAdd BLAST22
Topological domaini82 – 96LumenalSequence analysisAdd BLAST15
Transmembranei97 – 116HelicalSequence analysisAdd BLAST20
Topological domaini117 – 127CytoplasmicSequence analysisAdd BLAST11
Transmembranei128 – 147HelicalSequence analysisAdd BLAST20
Topological domaini148 – 166LumenalSequence analysisAdd BLAST19
Transmembranei167 – 186HelicalSequence analysisAdd BLAST20
Topological domaini187 – 196CytoplasmicSequence analysis10
Transmembranei197 – 213HelicalSequence analysisAdd BLAST17
Topological domaini214 – 223LumenalSequence analysis10
Transmembranei224 – 242HelicalSequence analysisAdd BLAST19
Topological domaini243 – 254CytoplasmicSequence analysisAdd BLAST12
Transmembranei255 – 271HelicalSequence analysisAdd BLAST17
Topological domaini272 – 276LumenalSequence analysis5
Transmembranei277 – 296HelicalSequence analysisAdd BLAST20
Topological domaini297 – 380CytoplasmicSequence analysisAdd BLAST84
Transmembranei381 – 399HelicalSequence analysisAdd BLAST19
Topological domaini400 – 410LumenalSequence analysisAdd BLAST11

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die 4 to 5 days post-fertilization, just after implantation, suggesting that protein function and N-glycosylation are essential in early embryogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087621 – 410UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi148N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP42867
PaxDbiP42867
PeptideAtlasiP42867
PRIDEiP42867

PTM databases

iPTMnetiP42867
PhosphoSitePlusiP42867

Expressioni

Developmental stagei

Highest activity is during the mid-phase of lactation.

Gene expression databases

BgeeiENSMUSG00000032123
ExpressionAtlasiP42867 baseline and differential
GenevisibleiP42867 MM

Interactioni

Protein-protein interaction databases

IntActiP42867, 1 interactor
STRINGi10090.ENSMUSP00000056282

Structurei

3D structure databases

ProteinModelPortaliP42867
SMRiP42867
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 81Dolichol recognitionAdd BLAST13
Motifi224 – 236Dolichol recognitionAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2788 Eukaryota
COG0472 LUCA
GeneTreeiENSGT00390000011424
HOGENOMiHOG000163915
HOVERGENiHBG000846
InParanoidiP42867
KOiK01001
OMAiGDYRDDH
OrthoDBiEOG091G0A0M
PhylomeDBiP42867
TreeFamiTF313734

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 1 hit
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit

Sequencei

Sequence statusi: Complete.

P42867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK
60 70 80 90 100
LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL
110 120 130 140 150
IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT
160 170 180 190 200
IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS
210 220 230 240 250
LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV
260 270 280 290 300
FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP
310 320 330 340 350
CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG
360 370 380 390 400
ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR
410
YQLVRLFYDV
Length:410
Mass (Da):46,412
Last modified:November 28, 2003 - v2
Checksum:iF729D6983FA67E0B
GO

RNA editingi

Edited at position 74.1 Publication
Partially edited.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti74C → Y in RNA edited version. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65603 mRNA Translation: CAA46553.1
BC010474 mRNA Translation: AAH10474.1
CCDSiCCDS23104.1
PIRiS24326
RefSeqiNP_031901.2, NM_007875.2
UniGeneiMm.18353

Genome annotation databases

EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123
GeneIDi13478
KEGGimmu:13478
UCSCiuc009pcv.1 mouse

Keywords - Coding sequence diversityi

RNA editing

Similar proteinsi

Entry informationi

Entry nameiGPT_MOUSE
AccessioniPrimary (citable) accession number: P42867
Secondary accession number(s): Q921W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 28, 2003
Last modified: April 25, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health