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P42867

- GPT_MOUSE

UniProt

P42867 - GPT_MOUSE

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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

Dpagt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi

GO - Molecular functioni

  1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: MGI
  4. UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity Source: MGI

GO - Biological processi

  1. dolichol biosynthetic process Source: Ensembl
  2. dolichol-linked oligosaccharide biosynthetic process Source: MGI
  3. protein N-linked glycosylation Source: Ensembl
  4. UDP-N-acetylglucosamine metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:Dpagt1
Synonyms:Dpagt2, Gnpta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1196396. Dpagt1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die 4 to 5 days post-fertilization, just after implantation, suggesting that protein function and N-glycosylation are essential in early embryogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP42867.
PaxDbiP42867.
PRIDEiP42867.

PTM databases

PhosphoSiteiP42867.

Expressioni

Developmental stagei

Highest activity is during the mid-phase of lactation.

Gene expression databases

BgeeiP42867.
ExpressionAtlasiP42867. baseline and differential.
GenevestigatoriP42867.

Structurei

3D structure databases

ProteinModelPortaliP42867.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66LumenalSequence Analysis
Topological domaini35 – 5925CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini82 – 9615LumenalSequence AnalysisAdd
BLAST
Topological domaini117 – 12711CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini148 – 16619LumenalSequence AnalysisAdd
BLAST
Topological domaini187 – 19610CytoplasmicSequence Analysis
Topological domaini214 – 22310LumenalSequence Analysis
Topological domaini243 – 25412CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini272 – 2765LumenalSequence Analysis
Topological domaini297 – 38084CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini400 – 41011LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 3428HelicalSequence AnalysisAdd
BLAST
Transmembranei60 – 8122HelicalSequence AnalysisAdd
BLAST
Transmembranei97 – 11620HelicalSequence AnalysisAdd
BLAST
Transmembranei128 – 14720HelicalSequence AnalysisAdd
BLAST
Transmembranei167 – 18620HelicalSequence AnalysisAdd
BLAST
Transmembranei197 – 21317HelicalSequence AnalysisAdd
BLAST
Transmembranei224 – 24219HelicalSequence AnalysisAdd
BLAST
Transmembranei255 – 27117HelicalSequence AnalysisAdd
BLAST
Transmembranei277 – 29620HelicalSequence AnalysisAdd
BLAST
Transmembranei381 – 39919HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 8113Dolichol recognitionAdd
BLAST
Motifi224 – 23613Dolichol recognitionAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiP42867.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG73804W.
PhylomeDBiP42867.
TreeFamiTF313734.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42867-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK
60 70 80 90 100
LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL
110 120 130 140 150
IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT
160 170 180 190 200
IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS
210 220 230 240 250
LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV
260 270 280 290 300
FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP
310 320 330 340 350
CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG
360 370 380 390 400
ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR
410
YQLVRLFYDV
Length:410
Mass (Da):46,412
Last modified:November 28, 2003 - v2
Checksum:iF729D6983FA67E0B
GO

RNA editingi

Partially edited.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti74 – 741C → Y in RNA edited version.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65603 mRNA. Translation: CAA46553.1.
BC010474 mRNA. Translation: AAH10474.1.
CCDSiCCDS23104.1.
PIRiS24326.
RefSeqiNP_031901.2. NM_007875.2.
UniGeneiMm.18353.

Genome annotation databases

EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123.
GeneIDi13478.
KEGGimmu:13478.
UCSCiuc009pcv.1. mouse.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65603 mRNA. Translation: CAA46553.1 .
BC010474 mRNA. Translation: AAH10474.1 .
CCDSi CCDS23104.1.
PIRi S24326.
RefSeqi NP_031901.2. NM_007875.2.
UniGenei Mm.18353.

3D structure databases

ProteinModelPortali P42867.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P42867.

Proteomic databases

MaxQBi P42867.
PaxDbi P42867.
PRIDEi P42867.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000054708 ; ENSMUSP00000056282 ; ENSMUSG00000032123 .
GeneIDi 13478.
KEGGi mmu:13478.
UCSCi uc009pcv.1. mouse.

Organism-specific databases

CTDi 1798.
MGIi MGI:1196396. Dpagt1.

Phylogenomic databases

eggNOGi COG0472.
GeneTreei ENSGT00390000011424.
HOGENOMi HOG000163915.
HOVERGENi HBG000846.
InParanoidi P42867.
KOi K01001.
OMAi IHERNLT.
OrthoDBi EOG73804W.
PhylomeDBi P42867.
TreeFami TF313734.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

ChiTaRSi DPAGT1. mouse.
NextBioi 283963.
PROi P42867.
SOURCEi Search...

Gene expression databases

Bgeei P42867.
ExpressionAtlasi P42867. baseline and differential.
Genevestigatori P42867.

Family and domain databases

InterProi IPR000715. Glycosyl_transferase_4.
[Graphical view ]
Pfami PF00953. Glycos_transf_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal localization."
    Rajput B., Ma J., Muniappa N., Schantz L., Naylor S.L., Lalley P.A., Vijay I.K.
    Biochem. J. 285:985-992(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  2. "Structure and organization of mouse GlcNAc-1-phosphate transferase gene."
    Rajput B., Ma J., Vijay I.K.
    J. Biol. Chem. 269:9590-9597(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING.
    Strain: BALB/c and NIH Swiss.
    Tissue: Liver and Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. "A recessive deletion in the GlcNAc-1-phosphotransferase gene results in peri-implantation embryonic lethality."
    Marek K.W., Vijay I.K., Marth J.D.
    Glycobiology 9:1263-1271(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGPT_MOUSE
AccessioniPrimary (citable) accession number: P42867
Secondary accession number(s): Q921W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 28, 2003
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3