P42866 (OPRM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mu-type opioid receptor
Short name=M-OR-1
Short name=MOR-1

Gene names

Name:	Oprm1
Synonyms:	Mor, Oprm

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
Subunit structure	Forms a complex with G(alpha)z/i2 subunits and the RGSZ proteins, RGSZ17 and RGSZ20. The formation of this complex results in mu-opioid receptor desensitization. Interacts with RGSZ17 and RGSZ20. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of adenylate cyclase activity by dopamine receptor signaling pathway Inferred from direct assay. Source: MGI inhibition of adenylate cyclase activity by G-protein signaling pathway Inferred from direct assay. Source: MGI locomotory behavior Inferred from genetic interaction. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	opioid receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Mu-type opioid receptor		PRO_0000069975
Regions
	Topological domain	1 – 64	64	Extracellular Potential
	Transmembrane	65 – 94	30	Helical; Name=1; Potential
	Topological domain	95 – 103	9	Cytoplasmic Potential
	Transmembrane	104 – 121	18	Helical; Name=2; Potential
	Topological domain	122 – 143	22	Extracellular Potential
	Transmembrane	144 – 163	20	Helical; Name=3; Potential
	Topological domain	164 – 193	30	Cytoplasmic Potential
	Transmembrane	194 – 209	16	Helical; Name=4; Potential
	Topological domain	210 – 234	25	Extracellular Potential
	Transmembrane	235 – 257	23	Helical; Name=5; Potential
	Topological domain	258 – 280	23	Cytoplasmic Potential
	Transmembrane	281 – 303	23	Helical; Name=6; Potential
	Topological domain	304 – 311	8	Extracellular Potential
	Transmembrane	312 – 328	17	Helical; Name=7; Potential
	Topological domain	329 – 398	70	Cytoplasmic Potential
Amino acid modifications
	Lipidation	351	1	S-palmitoyl cysteine Potential
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Disulfide bond	140 ↔ 217		By similarity
Experimental info
	Sequence conflict	22	1	C → W in AAA86878. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P42866 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DFE1C758E2DA197E	FASTA	398	44,421
	10 20 30 40 50 60 MDSSAGPGNI SDCSDPLAPA SCSPAPGSWL NLSHVDGNQS DPCGPNRTGL GGSHSLCPQT 70 80 90 100 110 120 GSPSMVTAIT IMALYSIVCV VGLFGNFLVM YVIVRYTKMK TATNIYIFNL ALADALATST 130 140 150 160 170 180 LPFQSVNYLM GTWPFGNILC KIVISIDYYN MFTSIFTLCT MSVDRYIAVC HPVKALDFRT 190 200 210 220 230 240 PRNAKIVNVC NWILSSAIGL PVMFMATTKY RQGSIDCTLT FSHPTWYWEN LLKICVFIFA 250 260 270 280 290 300 FIMPVLIITV CYGLMILRLK SVRMLSGSKE KDRNLRRITR MVLVVVAVFI VCWTPIHIYV 310 320 330 340 350 360 IIKALITIPE TTFQTVSWHF CIALGYTNSC LNPVLYAFLD ENFKRCFREF CIPTSSTIEQ 370 380 390 QNSARIRQNT REHPSTANTV DRTNHQLENL EAETAPLP

References

[1]	"Genomic structure analysis of promoter sequence of a mouse mu opioid receptor gene." Min B.H., Augustin L.B., Felsheim R.F., Fuchs J.A., Loh H.H. Proc. Natl. Acad. Sci. U.S.A. 91:9081-9085(1994) [PubMed: 8090773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6. Tissue: Liver.
[2]	"Antisense mapping the MOR-1 opioid receptor: evidence for alternative splicing and a novel morphine-6 beta-glucuronide receptor." Rossi G.C., Pan Y.X., Brown G.P., Pasternak G.W. FEBS Lett. 369:192-196(1995) [PubMed: 7649256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Characterization of the murine mu opioid receptor gene." Kaufman D.L., Keith D.E., Anton B., Tian J., Magendzo K., Newman D., Tran T., Lee D.S., Wen C., Xia Y., Lusis A.J., Evans C.J. J. Biol. Chem. 270:15877-15883(1995) [PubMed: 7797593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
[4]	"The RGSZ2 protein exists in a complex with mu-opioid receptors and regulates the desensitizing capacity of Gz proteins." Garzan J., Rodriguez-Munoz M., Lopez-Fando A., Sanchez-Blazquez P. Neuropsychopharmacology 30:1632-1648(2005) [PubMed: 15827571] [Abstract] Cited for: INTERACTION WITH RGS17 AND RGS20.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10561 , U10558, U10559, U10560 Genomic DNA. Translation: AAB60673.1. U26915 mRNA. Translation: AAA81170.1. U19380 mRNA. Translation: AAA86878.1.
IPI	IPI00831334.
PIR	A57510.
UniGene	Mm.457998.
3D structure databases
ProteinModelPortal	P42866.
SMR	P42866. Positions 69-352.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46148N.
STRING	P42866.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P42866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056385; ENSMUSP00000060590; ENSMUSG00000000766. ENSMUST00000092728; ENSMUSP00000090404; ENSMUSG00000000766. ENSMUST00000092734; ENSMUSP00000090410; ENSMUSG00000000766. ENSMUST00000105607; ENSMUSP00000101232; ENSMUSG00000000766.
Organism-specific databases
MGI	MGI:97441. Oprm1.
Phylogenomic databases
HOVERGEN	HBG106919.
Gene expression databases
ArrayExpress	P42866.
Bgee	P42866.
CleanEx	MM_OPRM1.
Genevestigator	P42866.
GermOnline	ENSMUSG00000000766. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000105. Mu_opioid_rcpt. IPR001418. Opioid_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00537. MUOPIOIDR. PR00384. OPIOIDR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

OPRM_MOUSE

Accession

Primary (citable) accession number: P42866
Secondary accession number(s): Q60768

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program