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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

NAGT

Organism
Leishmania amazonensis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Miscellaneous

In Leishmania sp., tunicamycin-resistant variants are associated with increased levels of NAGT gene transcription due to gene amplification.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Inhibited by tunicamycin.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:NAGT
OrganismiLeishmania amazonensis
Taxonomic identifieri5659 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Transmembranei316 – 336HelicalSequence analysisAdd BLAST21
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087631 – 466UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi416N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 2 hits
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit

Sequencei

Sequence statusi: Complete.

P42864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLGLVESSR NAAFAVAAHA PVLGLILLGS IVAYVGTLRY IPNVARTLLD
60 70 80 90 100
RNIFGIDINK STEEQRQKFA AKRRAGQTEE KEFQKQAIPE SLGILVGAMY
110 120 130 140 150
LSVVVVLTVC LRFLGAAGEG LDNPYASLPG PLMTITVMLL LGFVDDVLDV
160 170 180 190 200
KWRHKIILTA LGSLPLIMTY DGSLSVLMPC AFGRFGLSTM NVMKEWRLGL
210 220 230 240 250
AAPQGEPTTT FRATAPSTWF SFTVNHRSYV KVTESGAALI YLGPVYLVYL
260 270 280 290 300
SMLCIFCTNS INILAGVNGV EVGQSIVIAV ASVVYNLFQM RLDRQLTPDF
310 320 330 340 350
SSLDAAAADA RDMTSDHQLR ALLLLGPFIG VSLALWRYNR YPARVFVGDS
360 370 380 390 400
YTYFAGTVLA VSSITGVYSK TLLLFFAPQV FNFLISLPQL FSIVPCPRHR
410 420 430 440 450
VPTWNPRTNL LSNSHNYTIL NVILYLFGDM HEAKLTWAIL KCQVIACVLG
460
FVVRYVLSAF LYDEVR
Length:466
Mass (Da):51,352
Last modified:November 1, 1995 - v1
Checksum:i4C423662BA4072AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96635 Genomic DNA Translation: AAA29258.1
PIRiA44495
S27823

Similar proteinsi

Entry informationi

Entry nameiGPT_LEIAM
AccessioniPrimary (citable) accession number: P42864
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health