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P42864 (GPT_LEIAM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

EC=2.7.8.15
Alternative name(s):
GlcNAc-1-P transferase
Short name=G1PT
Short name=GPT
N-acetylglucosamine-1-phosphate transferase
Gene names
Name:NAGT
OrganismLeishmania amazonensis
Taxonomic identifier5659 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activity

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulation

Inhibited by tunicamycin.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Miscellaneous

In Leishmania sp., tunicamycin-resistant variants are associated with increased levels of NAGT gene transcription due to gene amplification.

Sequence similarities

Belongs to the glycosyltransferase 4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
PRO_0000108763

Regions

Transmembrane12 – 3221Helical; Potential
Transmembrane91 – 11121Helical; Potential
Transmembrane124 – 14421Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane236 – 25621Helical; Potential
Transmembrane263 – 28321Helical; Potential
Transmembrane316 – 33621Helical; Potential
Transmembrane345 – 36521Helical; Potential
Transmembrane374 – 39421Helical; Potential
Transmembrane442 – 46221Helical; Potential

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P42864 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4C423662BA4072AE

FASTA46651,352
        10         20         30         40         50         60 
MTLGLVESSR NAAFAVAAHA PVLGLILLGS IVAYVGTLRY IPNVARTLLD RNIFGIDINK 

        70         80         90        100        110        120 
STEEQRQKFA AKRRAGQTEE KEFQKQAIPE SLGILVGAMY LSVVVVLTVC LRFLGAAGEG 

       130        140        150        160        170        180 
LDNPYASLPG PLMTITVMLL LGFVDDVLDV KWRHKIILTA LGSLPLIMTY DGSLSVLMPC 

       190        200        210        220        230        240 
AFGRFGLSTM NVMKEWRLGL AAPQGEPTTT FRATAPSTWF SFTVNHRSYV KVTESGAALI 

       250        260        270        280        290        300 
YLGPVYLVYL SMLCIFCTNS INILAGVNGV EVGQSIVIAV ASVVYNLFQM RLDRQLTPDF 

       310        320        330        340        350        360 
SSLDAAAADA RDMTSDHQLR ALLLLGPFIG VSLALWRYNR YPARVFVGDS YTYFAGTVLA 

       370        380        390        400        410        420 
VSSITGVYSK TLLLFFAPQV FNFLISLPQL FSIVPCPRHR VPTWNPRTNL LSNSHNYTIL 

       430        440        450        460 
NVILYLFGDM HEAKLTWAIL KCQVIACVLG FVVRYVLSAF LYDEVR 

« Hide

References

[1]"The 63-kilobase circular amplicon of tunicamycin-resistant Leishmania amazonensis contains a functional N-acetylglucosamine-1-phosphate transferase gene that can be used as a dominant selectable marker in transfection."
Liu X., Chang K.-P.
Mol. Cell. Biol. 12:4112-4122(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MHOM/BR/73/LV78.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96635 Genomic DNA. Translation: AAA29258.1.
PIRA44495.
S27823.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPT_LEIAM
AccessionPrimary (citable) accession number: P42864
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways