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Protein

Umecyanin

Gene
N/A
Organism
Armoracia rusticana (Horseradish) (Armoracia laphatifolia)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable electron transfer copper protein that serves as a direct electron donor (PubMed:5089608, PubMed:11945593). Does not show any activity towards ascorbic acid, p-phenylenediamine and several common "classical" substrates for copper proteins (PubMed:5089608).2 Publications

Cofactori

Cu cation3 PublicationsNote: Binds 1 copper ion per subunit.1 Publication

Redox potential

E0 is +283 mV at pH 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Copper1 Publication
Metal bindingi85 – 851Copper1 Publication
Metal bindingi90 – 901Copper1 Publication
Metal bindingi95 – 951Copper1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Umecyanin1 Publication
Short name:
UMC1 Publication
OrganismiArmoracia rusticana (Horseradish) (Armoracia laphatifolia)
Taxonomic identifieri3704 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCardamineaeArmoracia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 115115UmecyaninPRO_0000085557Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 91PROSITE-ProRule annotation2 Publications
Glycosylationi76 – 761N-linked (GlcNAc...)1 Publication
Modified residuei113 – 1131Hydroxyproline1 Publication

Post-translational modificationi

Glycosylated at Asn-76 (PubMed:7757010). The carbohydrate content was determined to be 1.2%, corresponding to one hexose sugar per molecule (PubMed:5089608).2 Publications
Strongly heterogeneous at the C-terminus with the majority of the chains ending at position 106.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Structurei

Secondary structure

1
115
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Helixi7 – 93Combined sources
Helixi19 – 246Combined sources
Beta strandi34 – 374Combined sources
Turni41 – 433Combined sources
Beta strandi46 – 494Combined sources
Helixi51 – 555Combined sources
Beta strandi63 – 664Combined sources
Beta strandi68 – 747Combined sources
Beta strandi79 – 846Combined sources
Turni88 – 947Combined sources
Beta strandi96 – 1027Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X9RX-ray1.90A/B1-115[»]
1X9UX-ray1.80A/B1-115[»]
ProteinModelPortaliP42849.
SMRiP42849. Positions 1-104.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42849.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 103103PhytocyaninPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 1108Gly-rich

Sequence similaritiesi

Contains 1 phytocyanin domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR028871. BlueCu_1_BS.
IPR008972. Cupredoxin.
IPR003245. Phytocyanin_dom.
[Graphical view]
PfamiPF02298. Cu_bind_like. 1 hit.
[Graphical view]
ProDomiPD003122. Plcyanin-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS00196. COPPER_BLUE. 1 hit.
PS51485. PHYTOCYANIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
EDYDVGGDME WKRPSDPKFY ITWATGKTFR VGDELEFDFA AGMHDVAVVT
60 70 80 90 100
KDAFDNCKKE NPISHMTTPP VKIMLNTTGP QYYICTVGDH CRVGQKLSIN
110
VVGAGGAGGG ATPGA
Length:115
Mass (Da):12,372
Last modified:November 1, 1995 - v1
Checksum:i72B52EBDECAF1C6B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 481V → I in 25% of the molecules. 1 Publication

Sequence databases

PIRiA55827.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

PIRiA55827.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X9RX-ray1.90A/B1-115[»]
1X9UX-ray1.80A/B1-115[»]
ProteinModelPortaliP42849.
SMRiP42849. Positions 1-104.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP42849.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR028871. BlueCu_1_BS.
IPR008972. Cupredoxin.
IPR003245. Phytocyanin_dom.
[Graphical view]
PfamiPF02298. Cu_bind_like. 1 hit.
[Graphical view]
ProDomiPD003122. Plcyanin-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS00196. COPPER_BLUE. 1 hit.
PS51485. PHYTOCYANIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUMEC_ARMRU
AccessioniPrimary (citable) accession number: P42849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.