Reviewed, UniProtKB/Swiss-Prot P42847 (RT18_YEAST)

Names and origin

Protein names

Recommended name:
    37S ribosomal protein S18, mitochondrial
Alternative name(s):
    YmS18

Gene names

Name:	MRPS18
Ordered Locus Names:	YNL306W
ORF Names:	N0397

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5
Subcellular location	Mitochondrion.
Miscellaneous	Present with 5300 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ribosomal protein S11P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial translation Ref.5 Inferred by curator. Source: SGD
Cellular component	mitochondrial small ribosomal subunit Ref.5 Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Mitochondrion Ref.5
	Chain	60 – 217	158	37S ribosomal protein S18, mitochondrial		PRO_0000030586

Sequences

	Sequence		Length	Mass (Da)
	P42847-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F5B5225E4864A7FE	FASTA	217	24,563
	10 20 30 40 50 60 MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS 70 80 90 100 110 120 NGSSEVYKPK EEVVKYILHG KFTKNNTHLT FSSVVEDKNF HKNKGLTYND TMLYYLNLPQ 130 140 150 160 170 180 KVKISLSTGC LGFRKAARGE YEAAFQTSGR MFELIKEKNM LNKDIEVVMD DFGKGRAAFI 190 200 210 SALVGKEGAS VVKKVVKISD ATKLKFGGVR SPKMRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-198. Strain: S288c / FY1676.
[5]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed: 9151978] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-89, SUBUNIT. Strain: 07173.
[6]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed: 12392552] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49092.1. Z71582 Genomic DNA. Translation: CAA96234.1. Z46259 Genomic DNA. Translation: CAA86389.1. AY558132 Genomic DNA. Translation: AAS56458.1.
PIR	S60394.
RefSeq	NP_014093.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6794N.
IntAct	P42847. 10 interactions.
STRING	P42847.
Proteomic databases
PeptideAtlas	P42847.
PRIDE	P42847.
Genome annotation databases
Ensembl	YNL306W; YNL306W; YNL306W; Saccharomyces cerevisiae. [Genome view]
GeneID	855410.
KEGG	sce:YNL306W.
NMPDR	fig|4932.3.peg.5155.
Organism-specific databases
CYGD	YNL306w.
SGD	S000005250. MRPS18.
Phylogenomic databases
HOGENOM	P42847.
OMA	GQLGFRK
OrthoDB	EOG9FN60Z
Gene expression databases
ArrayExpress	P42847.
Genevestigator	P42847.
GermOnline	YNL306W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001971. Ribosomal_S11. [Graphical view]
Gene3D	G3DSA:3.30.420.80. Ribosomal_S11. 1 hit.
PROSITE	PS00054. RIBOSOMAL_S11. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979253.

Entry information

Entry name

RT18_YEAST

Accession

Primary (citable) accession number: P42847

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)