Reviewed, UniProtKB/Swiss-Prot P42846 (KRI1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein KRI1
Alternative name(s):
    KRR1-interacting protein 1

Gene names

Name:	KRI1
Ordered Locus Names:	YNL308C
ORF Names:	N0388

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA. Ref.3
Subunit structure	Interacts with KRR1. Ref.3
Subcellular location	Nucleus › nucleolus.
Post-translational modification	N-glycosylated.
Miscellaneous	Present with 1780 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the KRI1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	nucleolus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-28360,EBI-24597
KRR1	P25586	1	EBI-28360,EBI-21773

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Protein KRI1		PRO_0000203368
Regions
	Compositional bias	53 – 475	423	Glu-rich
Amino acid modifications
	Modified residue	177	1	Phosphoserine Ref.7 Ref.9
	Modified residue	184	1	Phosphoserine Ref.7 Ref.9
	Modified residue	185	1	Phosphoserine Ref.7
	Modified residue	482	1	Phosphotyrosine Ref.9
	Modified residue	486	1	Phosphoserine Ref.7 Ref.9 Ref.6 Ref.8
	Modified residue	489	1	Phosphoserine Ref.9 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P42846-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3B637571EDB05EA6	FASTA	591	68,654
	10 20 30 40 50 60 MPRKKSAAKR AREQAKKEAA VPATDTATIK TSETSATTVK PAIEASKSYV PSEDEEEDEE 70 80 90 100 110 120 EEEEEDDYGE LITDEVENGI NQVLDAIKNN KTDKLLDPKV KFFEDPESAA AKLANREGKH 130 140 150 160 170 180 KPIYLKDYHR MNILSGDALK EDDEEYEHAT VDGKQSFVSQ QREEKTQLLN EIKSAFSDEE 190 200 210 220 230 240 NEESSGDEDD GFLKKKEPST KKEGKNLPDP TVNEENFLEE FVNQQAWIPK KGDKVISLDL 250 260 270 280 290 300 NNNEEDDEEF EDAAEKFENA YNFRYEDPNA AEIISYARSQ ATLRRSDDSS RRRKREEKRK 310 320 330 340 350 360 IKEQIKAEKE TALQKKKTKK LNKLTDILEQ LTKEYGAEIN ADMVKKITDT LLKNDFKEEE 370 380 390 400 410 420 WDNVVAELFN EEFYQQEGKP TWNEDDEIMG DFYADADGDD QTEEGEVEKE QKEEDEEEGP 430 440 450 460 470 480 KRKKSKKEEK LQKKKEKRKV NELVENALEQ NKLALIEEVE KEEEERKSRS RTKEEQDLKF 490 500 510 520 530 540 RYREVSPESF GLTAREIFAA DDTDLNEFIG LKKFAPYRSK ELRAKDKRKV MKARRLREWR 550 560 570 580 590 KKTFKNENGL APVEAEAGEK DEDTILIPVE KASKSKHKRG HSHKHKGHQK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus." Sasaki T., Toh-e A., Kikuchi Y. Mol. Cell. Biol. 20:7971-7979(2000) [PubMed: 11027267] [Abstract] Cited for: FUNCTION, INTERACTION WITH KRR1, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177; SER-184; SER-185 AND SER-486, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486 AND SER-489, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177; SER-184; TYR-482; SER-486 AND SER-489, MASS SPECTROMETRY.
[10]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed: 19756047] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86387.1. Z71584 Genomic DNA. Translation: CAA96237.1.
PIR	S51303.
RefSeq	NP_014091.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4073N.
IntAct	P42846. 52 interactions.
STRING	P42846.
Proteomic databases
PeptideAtlas	P42846.
PRIDE	P42846.
Genome annotation databases
Ensembl	YNL308C; YNL308C; YNL308C; Saccharomyces cerevisiae. [Genome view]
GeneID	855408.
KEGG	sce:YNL308C.
NMPDR	fig|4932.3.peg.5153.
Organism-specific databases
CYGD	YNL308c.
SGD	S000005252. KRI1.
Phylogenomic databases
HOGENOM	P42846.
OMA	QAWIPKK
OrthoDB	EOG9N325D
Gene expression databases
ArrayExpress	P42846.
Genevestigator	P42846.
GermOnline	YNL308C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007851. Kri1_subgr. IPR018034. KRR1-interact_protein_1. [Graphical view]
PANTHER	PTHR14490. Krr1. 1 hit.
Pfam	PF05178. Kri1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979247.

Entry information

Entry name

KRI1_YEAST

Accession

Primary (citable) accession number: P42846

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)