Reviewed,
UniProtKB/Swiss-Prot P42845 (STB1_YEAST)
Last modified
November 24, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein STB1 Alternative name(s): SIN3-binding protein 1 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 420 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the regulation and timing of MBF-dependent transcription in late G1 of the cell cycle. Ref.5 |
| Subunit structure | Interacts with the ANK repeats of SWI6. The interaction with SWI6 is required for function. Interacts with SIN3. Ref.5 Ref.1 Ref.4 |
| Subcellular location | |
| Post-translational modification | Phosphorylated by CDC28 in a cell cycle-dependent manner, inhibiting the interaction with SWI6. Ref.4 Ref.9 Ref.10 Ref.11 Ref.12 |
| Miscellaneous | Present with 319 molecules/cell in log phase SD medium. Ref.8 |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | G1/S transition of mitotic cell cycle Ref.4 Inferred from expression pattern. Source: SGD |
| Cellular component | MBF transcription complex Inferred from physical interaction. Source: SGD SBF transcription complexInferred from physical interaction. Source: SGD Sin3-type complex Ref.1Inferred from direct assay. Source: SGD cytoplasm Ref.7Inferred from direct assay. Source: SGD |
| Molecular function | transcription activator activity Ref.5 Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 420 | 420 | Protein STB1 | PRO_0000072251 | |||||
Regions | |||||||||
| Region | 1 – 70 | 70 | Interaction with SWI6 | ||||||
| Compositional bias | 156 – 159 | 4 | Poly-Asn | ||||||
| Compositional bias | 165 – 169 | 5 | Poly-Asn | ||||||
Amino acid modifications | |||||||||
| Modified residue | 72 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 89 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 99 | 1 | Phosphothreonine Ref.10 Ref.11 Ref.12 | ||||||
| Modified residue | 102 | 1 | Phosphoserine Ref.10 Ref.11 Ref.12 | ||||||
| Modified residue | 253 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 419 | 1 | Phosphothreonine Ref.12 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins." Kasten M.M., Stillman D.J. Mol. Gen. Genet. 256:376-386(1997) [PubMed: 9393435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SIN3. |
| [2] | "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676. |
| [4] | "Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein." Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J. Mol. Cell. Biol. 19:5267-5278(1999) [PubMed: 10409718] [Abstract] Cited for: PROTEIN SEQUENCE OF 249-259, INTERACTION WITH SWI6, PHOSPHORYLATION BY CDC28. |
| [5] | "G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1." Costanzo M., Schub O., Andrews B.J. Mol. Cell. Biol. 23:5064-5077(2003) [PubMed: 12832490] [Abstract] Cited for: FUNCTION, INTERACTION WITH SWI6. |
| [6] | "Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE. |
| [7] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28. |
| [10] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY. |
| [11] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY. |
| [12] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-89; THR-99; SER-102; SER-253 AND THR-419, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U33439 Genomic DNA. Translation: AAA75482.1. Different initiation. Z46259 Genomic DNA. Translation: CAA86386.1. Different initiation. Z71585 Genomic DNA. Translation: CAA96238.1. Different initiation. | |
| PIR | S51302. |
| RefSeq | NP_014090.2. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2485N. |
| IntAct | P42845. 5 interactions. |
| STRING | P42845. |
Genome annotation databases | |
| Ensembl | YNL309W; YNL309W; YNL309W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 855407. |
| KEGG | sce:YNL309W. |
| NMPDR | fig|4932.3.peg.5152. |
Organism-specific databases | |
| CYGD | YNL309w. |
| SGD | S000005253. STB1. |
Phylogenomic databases | |
| HOGENOM | P42845. |
| OMA | NFNMGDY |
| OrthoDB | EOG9NP8KW |
Gene expression databases | |
| ArrayExpress | P42845. |
| Genevestigator | P42845. |
| GermOnline | YNL309W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 979244. |
Entry information
| Entry name | STB1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P42845 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

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