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P42845 (STB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein STB1
Alternative name(s):
SIN3-binding protein 1
Gene names
Name:STB1
Ordered Locus Names:YNL309W
ORF Names:N0384
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation and timing of MBF-dependent transcription in late G1 of the cell cycle. Ref.6

Subunit structure

Interacts with the ANK repeats of SWI6. The interaction with SWI6 is required for function. Interacts with SIN3. Ref.1 Ref.5 Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.8.

Post-translational modification

Phosphorylated by CDC28 in a cell cycle-dependent manner, inhibiting the interaction with SWI6. Ref.5 Ref.10

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.

Sequence caution

The sequence AAA75482.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA86386.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA96238.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 420419Protein STB1
PRO_0000072251

Regions

Region2 – 7069Interaction with SWI6
Compositional bias156 – 1594Poly-Asn
Compositional bias165 – 1695Poly-Asn

Amino acid modifications

Modified residue21N-acetylserine Ref.11
Modified residue71Phosphoserine Ref.11
Modified residue721Phosphoserine Ref.14
Modified residue991Phosphothreonine Ref.12 Ref.13 Ref.14
Modified residue1021Phosphoserine Ref.14
Modified residue4191Phosphothreonine Ref.13

Sequences

Sequence LengthMass (Da)Tools
P42845 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: B1F5E52C8A920400

FASTA42045,684
        10         20         30         40         50         60 
MSQPQMSPEK EQELASKILH RAELAQMTRQ LKLGLSNVPS TKRKQDSTTK KRSGEDAEDV 

        70         80         90        100        110        120 
DEDHKTLLEA ISPAKKPLHD DTNKMTVISP VKFVEKPNTP PSSRQRKAED RSQQIKPRKE 

       130        140        150        160        170        180 
DTPSTPRASA TPIILPHASS HYQRPHDKNF MTPKRNNNNS SNHSNNNNNI KKKAAGSKDA 

       190        200        210        220        230        240 
PQDSDNTAGA DLLMYLATSP YNKSSHHGTP MAVRMPTTPR SYHYASQLSL NGNTASTSND 

       250        260        270        280        290        300 
AVRFSHIKPS ASSPQSTFKS NLLPNFPDES LMDSPSLYLS NNNGSVQATL SPQQRRKPTT 

       310        320        330        340        350        360 
NTLHPPSNVP TTPSRELNGT NFNLLRTPNF NMGDYLHNLF SPSPRVPAQQ GASNTSASIP 

       370        380        390        400        410        420 
SVPAMVPGSS SNTSAIATAA ISSHTTNNFL DMNANGIPLI VGPGTDRIGE GESIDDKLTD 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins."
Kasten M.M., Stillman D.J.
Mol. Gen. Genet. 256:376-386(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SIN3.
[2]"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein."
Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J.
Mol. Cell. Biol. 19:5267-5278(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 249-259, INTERACTION WITH SWI6, PHOSPHORYLATION BY CDC28.
[6]"G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1."
Costanzo M., Schub O., Andrews B.J.
Mol. Cell. Biol. 23:5064-5077(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SWI6.
[7]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28.
[11]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[12]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND THR-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; THR-99 AND SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33439 Genomic DNA. Translation: AAA75482.1. Different initiation.
Z46259 Genomic DNA. Translation: CAA86386.1. Different initiation.
Z71585 Genomic DNA. Translation: CAA96238.1. Different initiation.
BK006947 Genomic DNA. Translation: DAA10252.1.
PIRS51302.
RefSeqNP_014090.2. NM_001183147.1.

3D structure databases

ProteinModelPortalP42845.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35530. 72 interactions.
DIPDIP-2485N.
IntActP42845. 4 interactions.
MINTMINT-533060.
STRING4932.YNL309W.

Proteomic databases

MaxQBP42845.
PaxDbP42845.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL309W; YNL309W; YNL309W.
GeneID855407.
KEGGsce:YNL309W.

Organism-specific databases

CYGDYNL309w.
SGDS000005253. STB1.

Phylogenomic databases

eggNOGNOG39630.
HOGENOMHOG000000865.
OMANFNMGDY.
OrthoDBEOG7C8GW8.

Enzyme and pathway databases

BioCycYEAST:G3O-33296-MONOMER.

Gene expression databases

GenevestigatorP42845.

Family and domain databases

ProtoNetSearch...

Other

NextBio979244.

Entry information

Entry nameSTB1_YEAST
AccessionPrimary (citable) accession number: P42845
Secondary accession number(s): D6W0N6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD