Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P42845

- STB1_YEAST

UniProt

P42845 - STB1_YEAST

Protein

Protein STB1

Gene

STB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (13 Sep 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the regulation and timing of MBF-dependent transcription in late G1 of the cell cycle.1 Publication

    GO - Molecular functioni

    1. transcription factor binding Source: SGD
    2. transcription factor binding transcription factor activity Source: SGD

    GO - Biological processi

    1. positive regulation of mating type switching by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    2. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33296-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein STB1
    Alternative name(s):
    SIN3-binding protein 1
    Gene namesi
    Name:STB1
    Ordered Locus Names:YNL309W
    ORF Names:N0384
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL309w.
    SGDiS000005253. STB1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. MBF transcription complex Source: SGD
    3. SBF transcription complex Source: SGD
    4. Sin3-type complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 420419Protein STB1PRO_0000072251Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei7 – 71Phosphoserine1 Publication
    Modified residuei72 – 721Phosphoserine1 Publication
    Modified residuei99 – 991Phosphothreonine3 Publications
    Modified residuei102 – 1021Phosphoserine1 Publication
    Modified residuei419 – 4191Phosphothreonine1 Publication

    Post-translational modificationi

    Phosphorylated by CDC28 in a cell cycle-dependent manner, inhibiting the interaction with SWI6.6 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP42845.
    PaxDbiP42845.

    Expressioni

    Gene expression databases

    GenevestigatoriP42845.

    Interactioni

    Subunit structurei

    Interacts with the ANK repeats of SWI6. The interaction with SWI6 is required for function. Interacts with SIN3.3 Publications

    Protein-protein interaction databases

    BioGridi35530. 72 interactions.
    DIPiDIP-2485N.
    IntActiP42845. 4 interactions.
    MINTiMINT-533060.
    STRINGi4932.YNL309W.

    Structurei

    3D structure databases

    ProteinModelPortaliP42845.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 7069Interaction with SWI6Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi156 – 1594Poly-Asn
    Compositional biasi165 – 1695Poly-Asn

    Phylogenomic databases

    eggNOGiNOG39630.
    HOGENOMiHOG000000865.
    OMAiNFNMGDY.
    OrthoDBiEOG7C8GW8.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42845-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQPQMSPEK EQELASKILH RAELAQMTRQ LKLGLSNVPS TKRKQDSTTK    50
    KRSGEDAEDV DEDHKTLLEA ISPAKKPLHD DTNKMTVISP VKFVEKPNTP 100
    PSSRQRKAED RSQQIKPRKE DTPSTPRASA TPIILPHASS HYQRPHDKNF 150
    MTPKRNNNNS SNHSNNNNNI KKKAAGSKDA PQDSDNTAGA DLLMYLATSP 200
    YNKSSHHGTP MAVRMPTTPR SYHYASQLSL NGNTASTSND AVRFSHIKPS 250
    ASSPQSTFKS NLLPNFPDES LMDSPSLYLS NNNGSVQATL SPQQRRKPTT 300
    NTLHPPSNVP TTPSRELNGT NFNLLRTPNF NMGDYLHNLF SPSPRVPAQQ 350
    GASNTSASIP SVPAMVPGSS SNTSAIATAA ISSHTTNNFL DMNANGIPLI 400
    VGPGTDRIGE GESIDDKLTD 420
    Length:420
    Mass (Da):45,684
    Last modified:September 13, 2005 - v2
    Checksum:iB1F5E52C8A920400
    GO

    Sequence cautioni

    The sequence AAA75482.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA86386.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA96238.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33439 Genomic DNA. Translation: AAA75482.1. Different initiation.
    Z46259 Genomic DNA. Translation: CAA86386.1. Different initiation.
    Z71585 Genomic DNA. Translation: CAA96238.1. Different initiation.
    BK006947 Genomic DNA. Translation: DAA10252.1.
    PIRiS51302.
    RefSeqiNP_014090.2. NM_001183147.1.

    Genome annotation databases

    EnsemblFungiiYNL309W; YNL309W; YNL309W.
    GeneIDi855407.
    KEGGisce:YNL309W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33439 Genomic DNA. Translation: AAA75482.1 . Different initiation.
    Z46259 Genomic DNA. Translation: CAA86386.1 . Different initiation.
    Z71585 Genomic DNA. Translation: CAA96238.1 . Different initiation.
    BK006947 Genomic DNA. Translation: DAA10252.1 .
    PIRi S51302.
    RefSeqi NP_014090.2. NM_001183147.1.

    3D structure databases

    ProteinModelPortali P42845.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35530. 72 interactions.
    DIPi DIP-2485N.
    IntActi P42845. 4 interactions.
    MINTi MINT-533060.
    STRINGi 4932.YNL309W.

    Proteomic databases

    MaxQBi P42845.
    PaxDbi P42845.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL309W ; YNL309W ; YNL309W .
    GeneIDi 855407.
    KEGGi sce:YNL309W.

    Organism-specific databases

    CYGDi YNL309w.
    SGDi S000005253. STB1.

    Phylogenomic databases

    eggNOGi NOG39630.
    HOGENOMi HOG000000865.
    OMAi NFNMGDY.
    OrthoDBi EOG7C8GW8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33296-MONOMER.

    Miscellaneous databases

    NextBioi 979244.

    Gene expression databases

    Genevestigatori P42845.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins."
      Kasten M.M., Stillman D.J.
      Mol. Gen. Genet. 256:376-386(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SIN3.
    2. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein."
      Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J.
      Mol. Cell. Biol. 19:5267-5278(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 249-259, INTERACTION WITH SWI6, PHOSPHORYLATION BY CDC28.
    6. "G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1."
      Costanzo M., Schub O., Andrews B.J.
      Mol. Cell. Biol. 23:5064-5077(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SWI6.
    7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. Cited for: PHOSPHORYLATION BY CDC28.
    11. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND THR-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; THR-99 AND SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTB1_YEAST
    AccessioniPrimary (citable) accession number: P42845
    Secondary accession number(s): D6W0N6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 319 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3