Protein STB1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P42845 (STB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein STB1

Alternative name(s):
SIN3-binding protein 1

Gene names

Name:	STB1
Ordered Locus Names:	YNL309W
ORF Names:	N0384

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation and timing of MBF-dependent transcription in late G1 of the cell cycle. Ref.6
Subunit structure	Interacts with the ANK repeats of SWI6. The interaction with SWI6 is required for function. Interacts with SIN3. Ref.1 Ref.5 Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.8.
Post-translational modification	Phosphorylated by CDC28 in a cell cycle-dependent manner, inhibiting the interaction with SWI6. Ref.5 Ref.10
Miscellaneous	Present with 319 molecules/cell in log phase SD medium.
Sequence caution	The sequence AAA75482.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA86386.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of mating type switching by positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction Ref.1. Source: SGD regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred from mutant phenotype PubMed 18794370. Source: SGD
Cellular_component	MBF transcription complex Inferred from physical interaction PubMed 18794370. Source: SGD SBF transcription complex Inferred from physical interaction PubMed 18794370. Source: SGD Sin3-type complex Inferred from direct assay Ref.1. Source: SGD cytoplasm Inferred from direct assay Ref.8. Source: SGD
Molecular_function	transcription factor binding transcription factor activity Inferred from mutant phenotype PubMed 19745812. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Protein STB1

PRO_0000072251

Regions

Region

1 – 70

Interaction with SWI6

Compositional bias

156 – 159

Poly-Asn

Compositional bias

165 – 169

Poly-Asn

Amino acid modifications

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphothreonine Ref.11 Ref.12 Ref.13

Modified residue

102

Phosphoserine Ref.11 Ref.12 Ref.13

Modified residue

253

Phosphoserine Ref.13

Modified residue

419

Phosphothreonine Ref.13

Sequences

Sequence

Length

Mass (Da)

Tools

P42845 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: B1F5E52C8A920400
Show »

FASTA

420

45,684

        10         20         30         40         50         60 
MSQPQMSPEK EQELASKILH RAELAQMTRQ LKLGLSNVPS TKRKQDSTTK KRSGEDAEDV 

        70         80         90        100        110        120 
DEDHKTLLEA ISPAKKPLHD DTNKMTVISP VKFVEKPNTP PSSRQRKAED RSQQIKPRKE 

       130        140        150        160        170        180 
DTPSTPRASA TPIILPHASS HYQRPHDKNF MTPKRNNNNS SNHSNNNNNI KKKAAGSKDA 

       190        200        210        220        230        240 
PQDSDNTAGA DLLMYLATSP YNKSSHHGTP MAVRMPTTPR SYHYASQLSL NGNTASTSND 

       250        260        270        280        290        300 
AVRFSHIKPS ASSPQSTFKS NLLPNFPDES LMDSPSLYLS NNNGSVQATL SPQQRRKPTT 

       310        320        330        340        350        360 
NTLHPPSNVP TTPSRELNGT NFNLLRTPNF NMGDYLHNLF SPSPRVPAQQ GASNTSASIP 

       370        380        390        400        410        420 
SVPAMVPGSS SNTSAIATAA ISSHTTNNFL DMNANGIPLI VGPGTDRIGE GESIDDKLTD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins." Kasten M.M., Stillman D.J. Mol. Gen. Genet. 256:376-386(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SIN3.
[2]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein." Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J. Mol. Cell. Biol. 19:5267-5278(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 249-259, INTERACTION WITH SWI6, PHOSPHORYLATION BY CDC28.
[6]	"G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1." Costanzo M., Schub O., Andrews B.J. Mol. Cell. Biol. 23:5064-5077(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SWI6.
[7]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-89; THR-99; SER-102; SER-253 AND THR-419, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33439 Genomic DNA. Translation: AAA75482.1. Different initiation. Z46259 Genomic DNA. Translation: CAA86386.1. Different initiation. Z71585 Genomic DNA. Translation: CAA96238.1. Different initiation. BK006947 Genomic DNA. Translation: DAA10252.1.
PIR	S51302.
RefSeq	NP_014090.2. NM_001183147.1.
3D structure databases
ProteinModelPortal	P42845.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2485N.
IntAct	P42845. 4 interactions.
MINT	MINT-533060.
STRING	4932.YNL309W.
Proteomic databases
PaxDb	P42845.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL309W; YNL309W; YNL309W.
GeneID	855407.
KEGG	sce:YNL309W.
Organism-specific databases
CYGD	YNL309w.
SGD	S000005253. STB1.
Phylogenomic databases
eggNOG	NOG39630.
HOGENOM	HOG000000865.
OMA	NFNMGDY.
OrthoDB	EOG4GXJWX.
Gene expression databases
Genevestigator	P42845.
GermOnline	YNL309W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979244.

Entry information

Entry name

STB1_YEAST

Accession

Primary (citable) accession number: P42845
Secondary accession number(s): D6W0N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 13, 2005
Last modified:	April 3, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents