P42845 (STB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein STB1

Alternative name(s):
SIN3-binding protein 1

Gene names

Name:	STB1
Ordered Locus Names:	YNL309W
ORF Names:	N0384

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation and timing of MBF-dependent transcription in late G1 of the cell cycle. Ref.6
Subunit structure	Interacts with the ANK repeats of SWI6. The interaction with SWI6 is required for function. Interacts with SIN3. Ref.1 Ref.5 Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.8.
Post-translational modification	Phosphorylated by CDC28 in a cell cycle-dependent manner, inhibiting the interaction with SWI6. Ref.5 Ref.10 Ref.11 Ref.12 Ref.13
Miscellaneous	Present with 319 molecules/cell in log phase SD medium. Ref.9
Sequence caution	The sequence AAA75482.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA86386.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred from mutant phenotype. Source: SGD
Cellular component	MBF transcription complex Inferred from physical interaction. Source: SGD SBF transcription complex Inferred from physical interaction. Source: SGD Sin3-type complex Inferred from direct assay Ref.1. Source: SGD cytoplasm Inferred from direct assay Ref.8. Source: SGD
Molecular function	transcription factor binding Inferred from physical interaction Ref.5Ref.6. Source: SGD transcription factor binding transcription factor activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Protein STB1		PRO_0000072251
Regions
	Region	1 – 70	70	Interaction with SWI6
	Compositional bias	156 – 159	4	Poly-Asn
	Compositional bias	165 – 169	5	Poly-Asn
Amino acid modifications
	Modified residue	72	1	Phosphoserine Ref.13
	Modified residue	89	1	Phosphoserine Ref.13
	Modified residue	99	1	Phosphothreonine Ref.11 Ref.12 Ref.13
	Modified residue	102	1	Phosphoserine Ref.11 Ref.12 Ref.13
	Modified residue	253	1	Phosphoserine Ref.13
	Modified residue	419	1	Phosphothreonine Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P42845 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: B1F5E52C8A920400	FASTA	420	45,684
	10 20 30 40 50 60 MSQPQMSPEK EQELASKILH RAELAQMTRQ LKLGLSNVPS TKRKQDSTTK KRSGEDAEDV 70 80 90 100 110 120 DEDHKTLLEA ISPAKKPLHD DTNKMTVISP VKFVEKPNTP PSSRQRKAED RSQQIKPRKE 130 140 150 160 170 180 DTPSTPRASA TPIILPHASS HYQRPHDKNF MTPKRNNNNS SNHSNNNNNI KKKAAGSKDA 190 200 210 220 230 240 PQDSDNTAGA DLLMYLATSP YNKSSHHGTP MAVRMPTTPR SYHYASQLSL NGNTASTSND 250 260 270 280 290 300 AVRFSHIKPS ASSPQSTFKS NLLPNFPDES LMDSPSLYLS NNNGSVQATL SPQQRRKPTT 310 320 330 340 350 360 NTLHPPSNVP TTPSRELNGT NFNLLRTPNF NMGDYLHNLF SPSPRVPAQQ GASNTSASIP 370 380 390 400 410 420 SVPAMVPGSS SNTSAIATAA ISSHTTNNFL DMNANGIPLI VGPGTDRIGE GESIDDKLTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins." Kasten M.M., Stillman D.J. Mol. Gen. Genet. 256:376-386(1997) [PubMed: 9393435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SIN3.
[2]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein." Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J. Mol. Cell. Biol. 19:5267-5278(1999) [PubMed: 10409718] [Abstract] Cited for: PROTEIN SEQUENCE OF 249-259, INTERACTION WITH SWI6, PHOSPHORYLATION BY CDC28.
[6]	"G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1." Costanzo M., Schub O., Andrews B.J. Mol. Cell. Biol. 23:5064-5077(2003) [PubMed: 12832490] [Abstract] Cited for: FUNCTION, INTERACTION WITH SWI6.
[7]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-99 AND SER-102, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-89; THR-99; SER-102; SER-253 AND THR-419, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33439 Genomic DNA. Translation: AAA75482.1. Different initiation. Z46259 Genomic DNA. Translation: CAA86386.1. Different initiation. Z71585 Genomic DNA. Translation: CAA96238.1. Different initiation. BK006947 Genomic DNA. Translation: DAA10252.1.
PIR	S51302.
RefSeq	NP_014090.2. NM_001183147.1.
3D structure databases
ProteinModelPortal	P42845.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2485N.
IntAct	P42845. 4 interactions.
MINT	MINT-533060.
STRING	P42845.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL309W; YNL309W; YNL309W.
GeneID	855407.
KEGG	sce:YNL309W.
NMPDR	fig|4932.3.peg.5152.
Organism-specific databases
CYGD	YNL309w.
SGD	S000005253. STB1.
Phylogenomic databases
eggNOG	fuNOG11004.
HOGENOM	HBG202699.
OMA	NFNMGDY.
OrthoDB	EOG4GXJWX.
Gene expression databases
ArrayExpress	P42845.
Genevestigator	P42845.
GermOnline	YNL309W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979244.

Entry information

Entry name

STB1_YEAST

Accession

Primary (citable) accession number: P42845
Secondary accession number(s): D6W0N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 13, 2005
Last modified:	December 14, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program