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Protein

F-box protein SKP2

Gene

SKP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins (By similarity). Regulates protein levels of sulfur metabolism enzymes. The SCF(SKP2) complex may regulate some transcription factors or regulators of cysteine and methionine biosynthesis.By similarity1 Publication

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • cellular protein catabolic process Source: SGD
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of sulfur amino acid metabolic process Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33298-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box protein SKP2
Gene namesi
Name:SKP2
Ordered Locus Names:YNL311C
ORF Names:N0376
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL311C.
SGDiS000005255. SKP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • SCF ubiquitin ligase complex Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Stabilizes the adenosylphosphosulfate kinase MET14 which leads to accumulation H2S and SO2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002033661 – 763F-box protein SKP2Add BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP42843.
PRIDEiP42843.

PTM databases

iPTMnetiP42843.

Interactioni

Subunit structurei

Interacts with SKP1. Component of the probable SCF(SKP2) complex containing CDC53, SKP1, RBX1 and SKP2. May interact with ribosomes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MET14Q021963EBI-28370,EBI-9485

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35528. 92 interactors.
DIPiDIP-1971N.
IntActiP42843. 5 interactors.
MINTiMINT-410026.
STRINGi4932.YNL311C.

Structurei

3D structure databases

ProteinModelPortaliP42843.
SMRiP42843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 100F-boxPROSITE-ProRule annotationAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Glu7

Phylogenomic databases

InParanoidiP42843.
OMAiLTIDCNS.
OrthoDBiEOG092C0MR8.

Family and domain databases

InterProiView protein in InterPro
IPR001810. F-box_dom.
PfamiView protein in Pfam
PF12937. F-box-like. 1 hit.
SMARTiView protein in SMART
SM00256. FBOX. 1 hit.
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiView protein in PROSITE
PS50181. FBOX. 1 hit.

Sequencei

Sequence statusi: Complete.

P42843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLQLFGRS KYFSLVSSAA KEEEEEEEGC ADAKSLLHST SHDIKSRSLR
60 70 80 90 100
FNDKSSLMCL PTKVLLLILR TLDFNTLVTL CQVNSRFYNL ITNEFLFQNV
110 120 130 140 150
ILDSKLSLLK FNALIHSEFH TSNIVTHSGD CSTQSRSQNA RFLVRSIEFK
160 170 180 190 200
NPQSQDSLLK YSKFYNKSGQ DSIIAGSYKL DSYDKDVKKL NNIRLNDETP
210 220 230 240 250
IITSERIKLL DKLESNYFHY TYIELMLDII DYLPNLTRVI LSDVEPNFKI
260 270 280 290 300
PLWYSVFNDG SRDFFKKIIK GQQSITNEDL RTFQLSKKFV KEYESKYYSL
310 320 330 340 350
PRLKILEIKA NNKRQRTFNR QRHHQKLVLR PSLFCCFGII NELKLENVTI
360 370 380 390 400
DTESLDTPME FLPLFLKNED NELYSLQSPI TALTLDSCDV VPGNGILRLF
410 420 430 440 450
HSYFKMVKHL SLLKINSKFD LLLCSCFPSL SNLTIDCNSK CFTNEQVVGE
460 470 480 490 500
SYYFQQRSLD TEDDFDDCNS MTETLFEAPS DSKIITPPPT SSVVLSLNLN
510 520 530 540 550
YISRTTGNDV SNNPSPDNNK KPAMLTAAQL QNFQRQRIPE FHSFYHYYRL
560 570 580 590 600
LWERLPSKNI SINVINIPFT NVYPLSPLSF WEHLARTITS VDETDEDVGD
610 620 630 640 650
ENDQETLIGY ENNSIRDNIP NANAVPNLST VMSPESDIHH TYYWNNSVRR
660 670 680 690 700
CLRDSLIKLK NRTIEYRDLD VEEFLQNVTL ENFFNDFQDP ENFKDIPNIN
710 720 730 740 750
LWCFLRNLSK FKAVKIRMLR HFSLCTPRTR YDWELLLKPV LRVNVPIEVR
760
DKDGFVLYSY GQK
Length:763
Mass (Da):88,942
Last modified:November 1, 1995 - v1
Checksum:i81102168449051BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16V → A in AAT92730 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA. Translation: CAA86384.1.
Z71587 Genomic DNA. Translation: CAA96240.1.
AY692711 Genomic DNA. Translation: AAT92730.1.
BK006947 Genomic DNA. Translation: DAA10250.1.
PIRiS51300.
RefSeqiNP_014088.1. NM_001183149.1.

Genome annotation databases

EnsemblFungiiYNL311C; YNL311C; YNL311C.
GeneIDi855405.
KEGGisce:YNL311C.

Similar proteinsi

Entry informationi

Entry nameiSKP2_YEAST
AccessioniPrimary (citable) accession number: P42843
Secondary accession number(s): D6W0N4, Q6B2L9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names