P42843 (SKP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein SKP2

Gene names

Name:	SKP2
Ordered Locus Names:	YNL311C
ORF Names:	N0376

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity. Regulates protein levels of sulfur metabolism enzymes. The SCF(SKP2) complex may regulate some transcription factors or regulators of cysteine and methionine biosynthesis. Ref.10
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(SKP2) complex containing CDC53, SKP1, RBX1 and SKP2. May interact with ribosomes. Ref.7 Ref.9
Subcellular location	Cytoplasm Ref.5.
Disruption phenotype	Stabilizes the adenosylphophosulfate kinase MET14 which leads to accumulation H2S and SO2. Ref.10
Miscellaneous	Present with 432 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular protein catabolic process Inferred from mutant phenotype Ref.10. Source: SGD protein ubiquitination Inferred from physical interaction. Source: GOC regulation of sulfur amino acid metabolic process Inferred from mutant phenotype Ref.10. Source: SGD
Cellular component	SCF ubiquitin ligase complex Inferred from direct assay Ref.7. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DMA1	P38823	2	EBI-28370,EBI-24686
DMA2	P53924	2	EBI-28370,EBI-28867
MET14	Q02196	3	EBI-28370,EBI-9485

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	F-box protein SKP2		PRO_0000203366
Regions
	Domain	54 – 100	47	F-box
	Compositional bias	22 – 28	7	Poly-Glu
Experimental info
	Sequence conflict	16	1	V → A in AAT92730. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P42843 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 81102168449051BC	FASTA	763	88,942
	10 20 30 40 50 60 MKRLQLFGRS KYFSLVSSAA KEEEEEEEGC ADAKSLLHST SHDIKSRSLR FNDKSSLMCL 70 80 90 100 110 120 PTKVLLLILR TLDFNTLVTL CQVNSRFYNL ITNEFLFQNV ILDSKLSLLK FNALIHSEFH 130 140 150 160 170 180 TSNIVTHSGD CSTQSRSQNA RFLVRSIEFK NPQSQDSLLK YSKFYNKSGQ DSIIAGSYKL 190 200 210 220 230 240 DSYDKDVKKL NNIRLNDETP IITSERIKLL DKLESNYFHY TYIELMLDII DYLPNLTRVI 250 260 270 280 290 300 LSDVEPNFKI PLWYSVFNDG SRDFFKKIIK GQQSITNEDL RTFQLSKKFV KEYESKYYSL 310 320 330 340 350 360 PRLKILEIKA NNKRQRTFNR QRHHQKLVLR PSLFCCFGII NELKLENVTI DTESLDTPME 370 380 390 400 410 420 FLPLFLKNED NELYSLQSPI TALTLDSCDV VPGNGILRLF HSYFKMVKHL SLLKINSKFD 430 440 450 460 470 480 LLLCSCFPSL SNLTIDCNSK CFTNEQVVGE SYYFQQRSLD TEDDFDDCNS MTETLFEAPS 490 500 510 520 530 540 DSKIITPPPT SSVVLSLNLN YISRTTGNDV SNNPSPDNNK KPAMLTAAQL QNFQRQRIPE 550 560 570 580 590 600 FHSFYHYYRL LWERLPSKNI SINVINIPFT NVYPLSPLSF WEHLARTITS VDETDEDVGD 610 620 630 640 650 660 ENDQETLIGY ENNSIRDNIP NANAVPNLST VMSPESDIHH TYYWNNSVRR CLRDSLIKLK 670 680 690 700 710 720 NRTIEYRDLD VEEFLQNVTL ENFFNDFQDP ENFKDIPNIN LWCFLRNLSK FKAVKIRMLR 730 740 750 760 HFSLCTPRTR YDWELLLKPV LRVNVPIEVR DKDGFVLYSY GQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(SKP2) COMPLEX.
[8]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed: 16702403] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking." Tagwerker C., Flick K., Cui M., Guerrero C., Dou Y., Auer B., Baldi P., Huang L., Kaiser P. Mol. Cell. Proteomics 5:737-748(2006) [PubMed: 16432255] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH SKP1.
[10]	"A novel mechanism regulates H(2) S and SO(2) production in Saccharomyces cerevisiae." Yoshida S., Imoto J., Minato T., Oouchi R., Kamada Y., Tomita M., Soga T., Yoshimoto H. Yeast 28:109-121(2011) [PubMed: 20936605] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION OF THE SCF(SKP2) COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86384.1. Z71587 Genomic DNA. Translation: CAA96240.1. AY692711 Genomic DNA. Translation: AAT92730.1. BK006947 Genomic DNA. Translation: DAA10250.1.
PIR	S51300.
RefSeq	NP_014088.1. NM_001183149.1.
3D structure databases
ProteinModelPortal	P42843.
SMR	P42843. Positions 55-104.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1971N.
IntAct	P42843. 12 interactions.
MINT	MINT-410026.
STRING	P42843.
Proteomic databases
PeptideAtlas	P42843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL311C; YNL311C; YNL311C.
GeneID	855405.
KEGG	sce:YNL311C.
NMPDR	fig|4932.3.peg.5150.
Organism-specific databases
CYGD	YNL311c.
SGD	S000005255. SKP2.
Phylogenomic databases
eggNOG	fuNOG05610.
HOGENOM	HBG202693.
OMA	YDWELLL.
OrthoDB	EOG4ZCXD4.
Gene expression databases
ArrayExpress	P42843.
Genevestigator	P42843.
GermOnline	YNL311C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979238.

Entry information

Entry name

SKP2_YEAST

Accession

Primary (citable) accession number: P42843
Secondary accession number(s): D6W0N4, Q6B2L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program