Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P42841 (PFS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyadenylation factor subunit 2
Gene names
Name:PFS2
Ordered Locus Names:YNL317W
ORF Names:N0348
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes. Ref.5

Subunit structure

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14. Ref.5 Ref.6

Subcellular location

Nucleus Ref.6 Ref.7.

Miscellaneous

Present with 7810 molecules/cell in log phase SD medium.

Sequence similarities

Contains 5 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Polyadenylation factor subunit 2
PRO_0000051122

Regions

Repeat133 – 16331WD 1
Repeat175 – 20531WD 2
Repeat217 – 24731WD 3
Repeat259 – 29032WD 4
Repeat348 – 37831WD 5

Experimental info

Sequence conflict21D → V in AAT93077. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P42841 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1D5A2C00F5F67104

FASTA46553,143
        10         20         30         40         50         60 
MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL PNLVVEPETS 

        70         80         90        100        110        120 
YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ WTPEGRRLVV ATYSGEFSLW 

       130        140        150        160        170        180 
NASSFTFETL MQAHDSAVTT MKYSHDSDWM ISGDADGMIK IWQPNFSMVK EIDAAHTESI 

       190        200        210        220        230        240 
RDMAFSSNDS KFVTCSDDNI LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD 

       250        260        270        280        290        300 
NLVKLWDPRS GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 

       310        320        330        340        350        360 
VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD KCITSLSYNP 

       370        380        390        400        410        420 
VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING WFFGINNDIN AVREKSEFGA 

       430        440        450        460 
APPPPATLEP HALPNMNGFI NKKPRQEIPG IDSNIKSSTL PGLSI 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 83-91, FUNCTION, IDENTIFICATION IN THE CPF COMPLEX, INTERACTION WITH YSH1; FIP1 AND RNA14.
[6]"Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46259 Genomic DNA. Translation: CAA86378.1.
Z71593 Genomic DNA. Translation: CAA96247.1.
AY693058 Genomic DNA. Translation: AAT93077.1.
BK006947 Genomic DNA. Translation: DAA10244.1.
PIRS51295.
RefSeqNP_014082.1. NM_001183155.1.

3D structure databases

ProteinModelPortalP42841.
SMRP42841. Positions 47-382.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35522. 32 interactions.
DIPDIP-5816N.
IntActP42841. 19 interactions.
MINTMINT-381244.
STRING4932.YNL317W.

Proteomic databases

PaxDbP42841.
PeptideAtlasP42841.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL317W; YNL317W; YNL317W.
GeneID855399.
KEGGsce:YNL317W.

Organism-specific databases

CYGDYNL317w.
SGDS000005261. PFS2.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00730000111130.
HOGENOMHOG000213918.
KOK15542.
OMARTIRFWT.
OrthoDBEOG7DC2DB.

Enzyme and pathway databases

BioCycYEAST:G3O-33303-MONOMER.

Gene expression databases

GenevestigatorP42841.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 4 hits.
[Graphical view]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979220.

Entry information

Entry namePFS2_YEAST
AccessionPrimary (citable) accession number: P42841
Secondary accession number(s): D6W0M8, Q6B1M2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families