ID   PFS2_YEAST              Reviewed;         465 AA.
AC   P42841; D6W0M8; Q6B1M2;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 180.
DE   RecName: Full=Polyadenylation factor subunit 2;
GN   Name=PFS2; OrderedLocusNames=YNL317W; ORFNames=N0348;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / FY1676;
RX   PubMed=7502583; DOI=10.1002/yea.320111109;
RA   Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.;
RT   "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV
RT   identifies six known genes, a new member of the hexose transporter family
RT   and ten new open reading frames.";
RL   Yeast 11:1077-1085(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   PROTEIN SEQUENCE OF 83-91, FUNCTION, IDENTIFICATION IN THE CPF COMPLEX, AND
RP   INTERACTION WITH YSH1; FIP1 AND RNA14.
RX   PubMed=10619842; DOI=10.1093/emboj/19.1.37;
RA   Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.;
RT   "The WD-repeat protein pfs2p bridges two essential factors within the yeast
RT   pre-mRNA 3'-end-processing complex.";
RL   EMBO J. 19:37-47(2000).
RN   [6]
RP   IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY.
RX   PubMed=12819204; DOI=10.1074/jbc.m304454200;
RA   Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T.,
RA   Buratowski S., Moore C.L., Greenblatt J.;
RT   "Organization and function of APT, a subcomplex of the yeast cleavage and
RT   polyadenylation factor involved in the formation of mRNA and small
RT   nucleolar RNA 3'-ends.";
RL   J. Biol. Chem. 278:33000-33010(2003).
RN   [7]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Integral and essential component of the cleavage and
CC       polyadenylation factor (CPF) complex, which plays a key role in
CC       polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with
CC       cleavage factors including the CFIA complex and NAB4/CFIB. May bridge
CC       the CPF and CFIA complexes. {ECO:0000269|PubMed:10619842}.
CC   -!- SUBUNIT: Component of the cleavage and polyadenylation factor (CPF)
CC       complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1,
CC       REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and
CC       PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14.
CC       {ECO:0000269|PubMed:10619842, ECO:0000269|PubMed:12819204}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12819204,
CC       ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 7810 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z46259; CAA86378.1; -; Genomic_DNA.
DR   EMBL; Z71593; CAA96247.1; -; Genomic_DNA.
DR   EMBL; AY693058; AAT93077.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10244.1; -; Genomic_DNA.
DR   PIR; S51295; S51295.
DR   RefSeq; NP_014082.1; NM_001183155.1.
DR   PDB; 6EOJ; EM; 3.55 A; D=1-465.
DR   PDB; 7ZGP; EM; 2.70 A; D=1-465.
DR   PDB; 7ZGQ; EM; 2.80 A; D=1-465.
DR   PDB; 7ZGR; EM; 2.60 A; D=1-465.
DR   PDBsum; 6EOJ; -.
DR   PDBsum; 7ZGP; -.
DR   PDBsum; 7ZGQ; -.
DR   PDBsum; 7ZGR; -.
DR   AlphaFoldDB; P42841; -.
DR   EMDB; EMD-3908; -.
DR   SMR; P42841; -.
DR   BioGRID; 35522; 44.
DR   ComplexPortal; CPX-1053; Cleavage and polyadenylation specificity factor complex.
DR   DIP; DIP-5816N; -.
DR   IntAct; P42841; 33.
DR   MINT; P42841; -.
DR   STRING; 4932.YNL317W; -.
DR   MaxQB; P42841; -.
DR   PaxDb; 4932-YNL317W; -.
DR   PeptideAtlas; P42841; -.
DR   EnsemblFungi; YNL317W_mRNA; YNL317W; YNL317W.
DR   GeneID; 855399; -.
DR   KEGG; sce:YNL317W; -.
DR   AGR; SGD:S000005261; -.
DR   SGD; S000005261; PFS2.
DR   VEuPathDB; FungiDB:YNL317W; -.
DR   eggNOG; KOG0284; Eukaryota.
DR   GeneTree; ENSGT00730000111130; -.
DR   HOGENOM; CLU_000288_77_0_1; -.
DR   InParanoid; P42841; -.
DR   OMA; HHWDVKS; -.
DR   OrthoDB; 5487329at2759; -.
DR   BioCyc; YEAST:G3O-33303-MONOMER; -.
DR   Reactome; R-SCE-77595; Processing of Intronless Pre-mRNAs.
DR   BioGRID-ORCS; 855399; 6 hits in 10 CRISPR screens.
DR   PRO; PR:P42841; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P42841; Protein.
DR   GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; NAS:ComplexPortal.
DR   GO; GO:0006378; P:mRNA polyadenylation; IDA:ComplexPortal.
DR   GO; GO:0006397; P:mRNA processing; IDA:SGD.
DR   GO; GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; NAS:ComplexPortal.
DR   CDD; cd00200; WD40; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 3.
DR   InterPro; IPR045245; Pfs2-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR22836:SF0; PRE-MRNA 3' END PROCESSING PROTEIN WDR33; 1.
DR   PANTHER; PTHR22836; WD40 REPEAT PROTEIN; 1.
DR   Pfam; PF00400; WD40; 4.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; mRNA processing; Nucleus;
KW   Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..465
FT                   /note="Polyadenylation factor subunit 2"
FT                   /id="PRO_0000051122"
FT   REPEAT          133..163
FT                   /note="WD 1"
FT   REPEAT          175..205
FT                   /note="WD 2"
FT   REPEAT          217..247
FT                   /note="WD 3"
FT   REPEAT          259..290
FT                   /note="WD 4"
FT   REPEAT          348..378
FT                   /note="WD 5"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          417..465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        451..465
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        2
FT                   /note="D -> V (in Ref. 4; AAT93077)"
FT                   /evidence="ECO:0000305"
FT   HELIX           36..47
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   HELIX           59..64
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   HELIX           73..79
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          83..88
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          98..101
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          105..111
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          117..121
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            122..124
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          138..143
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          150..154
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          160..162
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          168..170
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          175..178
FT                   /evidence="ECO:0007829|PDB:7ZGP"
FT   STRAND          180..185
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          187..205
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            206..208
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          211..216
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          222..227
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          229..232
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          234..238
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          243..246
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            248..250
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          253..257
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          264..269
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            271..273
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          276..281
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            282..284
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          285..290
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   HELIX           291..293
FT                   /evidence="ECO:0007829|PDB:7ZGP"
FT   STRAND          296..302
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          307..312
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          314..316
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          319..324
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          327..333
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            334..336
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          337..340
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          342..345
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          348..351
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          353..358
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          362..369
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          374..378
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            385..389
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   TURN            391..393
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          399..402
FT                   /evidence="ECO:0007829|PDB:7ZGP"
FT   HELIX           403..407
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          409..412
FT                   /evidence="ECO:0007829|PDB:7ZGR"
FT   STRAND          417..419
FT                   /evidence="ECO:0007829|PDB:7ZGR"
SQ   SEQUENCE   465 AA;  53143 MW;  1D5A2C00F5F67104 CRC64;
     MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL PNLVVEPETS
     YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ WTPEGRRLVV ATYSGEFSLW
     NASSFTFETL MQAHDSAVTT MKYSHDSDWM ISGDADGMIK IWQPNFSMVK EIDAAHTESI
     RDMAFSSNDS KFVTCSDDNI LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD
     NLVKLWDPRS GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC
     VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD KCITSLSYNP
     VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING WFFGINNDIN AVREKSEFGA
     APPPPATLEP HALPNMNGFI NKKPRQEIPG IDSNIKSSTL PGLSI
//