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P42841

- PFS2_YEAST

UniProt

P42841 - PFS2_YEAST

Protein

Polyadenylation factor subunit 2

Gene

PFS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes.1 Publication

    GO - Biological processi

    1. mRNA cleavage Source: SGD
    2. mRNA polyadenylation Source: SGD

    Keywords - Biological processi

    mRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33303-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyadenylation factor subunit 2
    Gene namesi
    Name:PFS2
    Ordered Locus Names:YNL317W
    ORF Names:N0348
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL317w.
    SGDiS000005261. PFS2.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. mRNA cleavage and polyadenylation specificity factor complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 465465Polyadenylation factor subunit 2PRO_0000051122Add
    BLAST

    Proteomic databases

    MaxQBiP42841.
    PaxDbiP42841.
    PeptideAtlasiP42841.

    Expressioni

    Gene expression databases

    GenevestigatoriP42841.

    Interactioni

    Subunit structurei

    Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14.2 Publications

    Protein-protein interaction databases

    BioGridi35522. 33 interactions.
    DIPiDIP-5816N.
    IntActiP42841. 19 interactions.
    MINTiMINT-381244.
    STRINGi4932.YNL317W.

    Structurei

    3D structure databases

    ProteinModelPortaliP42841.
    SMRiP42841. Positions 47-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati133 – 16331WD 1Add
    BLAST
    Repeati175 – 20531WD 2Add
    BLAST
    Repeati217 – 24731WD 3Add
    BLAST
    Repeati259 – 29032WD 4Add
    BLAST
    Repeati348 – 37831WD 5Add
    BLAST

    Sequence similaritiesi

    Contains 5 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00730000111130.
    HOGENOMiHOG000213918.
    KOiK15542.
    OMAiRTIRFWT.
    OrthoDBiEOG7DC2DB.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 4 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42841-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL    50
    PNLVVEPETS YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ 100
    WTPEGRRLVV ATYSGEFSLW NASSFTFETL MQAHDSAVTT MKYSHDSDWM 150
    ISGDADGMIK IWQPNFSMVK EIDAAHTESI RDMAFSSNDS KFVTCSDDNI 200
    LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD NLVKLWDPRS 250
    GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 300
    VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD 350
    KCITSLSYNP VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING 400
    WFFGINNDIN AVREKSEFGA APPPPATLEP HALPNMNGFI NKKPRQEIPG 450
    IDSNIKSSTL PGLSI 465
    Length:465
    Mass (Da):53,143
    Last modified:November 1, 1995 - v1
    Checksum:i1D5A2C00F5F67104
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21D → V in AAT93077. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86378.1.
    Z71593 Genomic DNA. Translation: CAA96247.1.
    AY693058 Genomic DNA. Translation: AAT93077.1.
    BK006947 Genomic DNA. Translation: DAA10244.1.
    PIRiS51295.
    RefSeqiNP_014082.1. NM_001183155.1.

    Genome annotation databases

    EnsemblFungiiYNL317W; YNL317W; YNL317W.
    GeneIDi855399.
    KEGGisce:YNL317W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86378.1 .
    Z71593 Genomic DNA. Translation: CAA96247.1 .
    AY693058 Genomic DNA. Translation: AAT93077.1 .
    BK006947 Genomic DNA. Translation: DAA10244.1 .
    PIRi S51295.
    RefSeqi NP_014082.1. NM_001183155.1.

    3D structure databases

    ProteinModelPortali P42841.
    SMRi P42841. Positions 47-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35522. 33 interactions.
    DIPi DIP-5816N.
    IntActi P42841. 19 interactions.
    MINTi MINT-381244.
    STRINGi 4932.YNL317W.

    Proteomic databases

    MaxQBi P42841.
    PaxDbi P42841.
    PeptideAtlasi P42841.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL317W ; YNL317W ; YNL317W .
    GeneIDi 855399.
    KEGGi sce:YNL317W.

    Organism-specific databases

    CYGDi YNL317w.
    SGDi S000005261. PFS2.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00730000111130.
    HOGENOMi HOG000213918.
    KOi K15542.
    OMAi RTIRFWT.
    OrthoDBi EOG7DC2DB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33303-MONOMER.

    Miscellaneous databases

    NextBioi 979220.

    Gene expression databases

    Genevestigatori P42841.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 4 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
      Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
      EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 83-91, FUNCTION, IDENTIFICATION IN THE CPF COMPLEX, INTERACTION WITH YSH1; FIP1 AND RNA14.
    6. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
      Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
      J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPFS2_YEAST
    AccessioniPrimary (citable) accession number: P42841
    Secondary accession number(s): D6W0M8, Q6B1M2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7810 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3