Reviewed, UniProtKB/Swiss-Prot P42841 (PFS2_YEAST)

Names and origin

Protein names

Recommended name:
    Polyadenylation factor subunit 2

Gene names

Name:	PFS2
Ordered Locus Names:	YNL317W
ORF Names:	N0348

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes. Ref.4
Subunit structure	Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14. Ref.4
Subcellular location	Nucleus.
Miscellaneous	Present with 7810 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 5 WD repeats.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mRNA cleavage Ref.4 Inferred from direct assay. Source: SGD mRNA polyadenylation Ref.4 Inferred from direct assay. Source: SGD
Cellular component	mRNA cleavage and polyadenylation specificity factor complex Ref.5 Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCT2	P39076	1	EBI-28378,EBI-19049

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Polyadenylation factor subunit 2		PRO_0000051122
Regions
	Repeat	133 – 163	31	WD 1
	Repeat	175 – 205	31	WD 2
	Repeat	217 – 247	31	WD 3
	Repeat	259 – 290	32	WD 4
	Repeat	348 – 378	31	WD 5
Experimental info
	Sequence conflict	2	1	D → V in AAT93077. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P42841-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1D5A2C00F5F67104	FASTA	465	53,143
	10 20 30 40 50 60 MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL PNLVVEPETS 70 80 90 100 110 120 YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ WTPEGRRLVV ATYSGEFSLW 130 140 150 160 170 180 NASSFTFETL MQAHDSAVTT MKYSHDSDWM ISGDADGMIK IWQPNFSMVK EIDAAHTESI 190 200 210 220 230 240 RDMAFSSNDS KFVTCSDDNI LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD 250 260 270 280 290 300 NLVKLWDPRS GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 310 320 330 340 350 360 VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD KCITSLSYNP 370 380 390 400 410 420 VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING WFFGINNDIN AVREKSEFGA 430 440 450 460 APPPPATLEP HALPNMNGFI NKKPRQEIPG IDSNIKSSTL PGLSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex." Ohnacker M., Barabino S.M.L., Preker P.J., Keller W. EMBO J. 19:37-47(2000) [PubMed: 10619842] [Abstract] Cited for: PROTEIN SEQUENCE OF 83-91, FUNCTION, IDENTIFICATION IN THE CPF COMPLEX, INTERACTION WITH YSH1; FIP1 AND RNA14.
[5]	"Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends." Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J. J. Biol. Chem. 278:33000-33010(2003) [PubMed: 12819204] [Abstract] Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86378.1. Z71593 Genomic DNA. Translation: CAA96247.1. AY693058 Genomic DNA. Translation: AAT93077.1.
PIR	S51295.
RefSeq	NP_014082.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5816N.
IntAct	P42841. 27 interactions.
STRING	P42841.
Proteomic databases
PeptideAtlas	P42841.
Genome annotation databases
Ensembl	YNL317W; YNL317W; YNL317W; Saccharomyces cerevisiae. [Genome view]
GeneID	855399.
KEGG	sce:YNL317W.
NMPDR	fig|4932.3.peg.5144.
Organism-specific databases
CYGD	YNL317w.
SGD	S000005261. PFS2.
Phylogenomic databases
HOGENOM	P42841.
OMA	NGMTFNF
OrthoDB	EOG9P2RJC
Gene expression databases
ArrayExpress	P42841.
Genevestigator	P42841.
GermOnline	YNL317W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979220.

Entry information

Entry name

PFS2_YEAST

Accession

Primary (citable) accession number: P42841
Secondary accession number(s): Q6B1M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)