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Protein

Polyadenylation factor subunit 2

Gene

PFS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes.1 Publication

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyadenylation factor subunit 2
Gene namesi
Name:PFS2
Ordered Locus Names:YNL317W
ORF Names:N0348
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL317W.
SGDiS000005261. PFS2.

Subcellular locationi

GO - Cellular componenti

  • mRNA cleavage and polyadenylation specificity factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511221 – 465Polyadenylation factor subunit 2Add BLAST465

Proteomic databases

MaxQBiP42841.
PRIDEiP42841.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14.2 Publications

Protein-protein interaction databases

BioGridi35522. 34 interactors.
DIPiDIP-5816N.
IntActiP42841. 20 interactors.
MINTiMINT-381244.

Structurei

3D structure databases

ProteinModelPortaliP42841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati133 – 163WD 1Add BLAST31
Repeati175 – 205WD 2Add BLAST31
Repeati217 – 247WD 3Add BLAST31
Repeati259 – 290WD 4Add BLAST32
Repeati348 – 378WD 5Add BLAST31

Sequence similaritiesi

Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00730000111130.
HOGENOMiHOG000213918.
InParanoidiP42841.
KOiK15542.
OMAiRVLSGHH.
OrthoDBiEOG092C10HM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL
60 70 80 90 100
PNLVVEPETS YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ
110 120 130 140 150
WTPEGRRLVV ATYSGEFSLW NASSFTFETL MQAHDSAVTT MKYSHDSDWM
160 170 180 190 200
ISGDADGMIK IWQPNFSMVK EIDAAHTESI RDMAFSSNDS KFVTCSDDNI
210 220 230 240 250
LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD NLVKLWDPRS
260 270 280 290 300
GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC
310 320 330 340 350
VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD
360 370 380 390 400
KCITSLSYNP VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING
410 420 430 440 450
WFFGINNDIN AVREKSEFGA APPPPATLEP HALPNMNGFI NKKPRQEIPG
460
IDSNIKSSTL PGLSI
Length:465
Mass (Da):53,143
Last modified:November 1, 1995 - v1
Checksum:i1D5A2C00F5F67104
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2D → V in AAT93077 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA. Translation: CAA86378.1.
Z71593 Genomic DNA. Translation: CAA96247.1.
AY693058 Genomic DNA. Translation: AAT93077.1.
BK006947 Genomic DNA. Translation: DAA10244.1.
PIRiS51295.
RefSeqiNP_014082.1. NM_001183155.1.

Genome annotation databases

EnsemblFungiiYNL317W; YNL317W; YNL317W.
GeneIDi855399.
KEGGisce:YNL317W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA. Translation: CAA86378.1.
Z71593 Genomic DNA. Translation: CAA96247.1.
AY693058 Genomic DNA. Translation: AAT93077.1.
BK006947 Genomic DNA. Translation: DAA10244.1.
PIRiS51295.
RefSeqiNP_014082.1. NM_001183155.1.

3D structure databases

ProteinModelPortaliP42841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35522. 34 interactors.
DIPiDIP-5816N.
IntActiP42841. 20 interactors.
MINTiMINT-381244.

Proteomic databases

MaxQBiP42841.
PRIDEiP42841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL317W; YNL317W; YNL317W.
GeneIDi855399.
KEGGisce:YNL317W.

Organism-specific databases

EuPathDBiFungiDB:YNL317W.
SGDiS000005261. PFS2.

Phylogenomic databases

GeneTreeiENSGT00730000111130.
HOGENOMiHOG000213918.
InParanoidiP42841.
KOiK15542.
OMAiRVLSGHH.
OrthoDBiEOG092C10HM.

Enzyme and pathway databases

BioCyciYEAST:G3O-33303-MONOMER.

Miscellaneous databases

PROiP42841.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFS2_YEAST
AccessioniPrimary (citable) accession number: P42841
Secondary accession number(s): D6W0M8, Q6B1M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.