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P42841

- PFS2_YEAST

UniProt

P42841 - PFS2_YEAST

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Protein
Polyadenylation factor subunit 2
Gene
PFS2, YNL317W, N0348
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes.1 Publication

GO - Biological processi

  1. mRNA cleavage Source: SGD
  2. mRNA polyadenylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyadenylation factor subunit 2
Gene namesi
Name:PFS2
Ordered Locus Names:YNL317W
ORF Names:N0348
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL317w.
SGDiS000005261. PFS2.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Polyadenylation factor subunit 2
PRO_0000051122Add
BLAST

Proteomic databases

MaxQBiP42841.
PaxDbiP42841.
PeptideAtlasiP42841.

Expressioni

Gene expression databases

GenevestigatoriP42841.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with YSH1/BRR5, FIP1 and RNA14.2 Publications

Protein-protein interaction databases

BioGridi35522. 33 interactions.
DIPiDIP-5816N.
IntActiP42841. 19 interactions.
MINTiMINT-381244.
STRINGi4932.YNL317W.

Structurei

3D structure databases

ProteinModelPortaliP42841.
SMRiP42841. Positions 47-382.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati133 – 16331WD 1
Add
BLAST
Repeati175 – 20531WD 2
Add
BLAST
Repeati217 – 24731WD 3
Add
BLAST
Repeati259 – 29032WD 4
Add
BLAST
Repeati348 – 37831WD 5
Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00730000111130.
HOGENOMiHOG000213918.
KOiK15542.
OMAiRTIRFWT.
OrthoDBiEOG7DC2DB.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42841-1 [UniParc]FASTAAdd to Basket

« Hide

MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL    50
PNLVVEPETS YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ 100
WTPEGRRLVV ATYSGEFSLW NASSFTFETL MQAHDSAVTT MKYSHDSDWM 150
ISGDADGMIK IWQPNFSMVK EIDAAHTESI RDMAFSSNDS KFVTCSDDNI 200
LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD NLVKLWDPRS 250
GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 300
VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD 350
KCITSLSYNP VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING 400
WFFGINNDIN AVREKSEFGA APPPPATLEP HALPNMNGFI NKKPRQEIPG 450
IDSNIKSSTL PGLSI 465
Length:465
Mass (Da):53,143
Last modified:November 1, 1995 - v1
Checksum:i1D5A2C00F5F67104
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21D → V in AAT93077. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86378.1.
Z71593 Genomic DNA. Translation: CAA96247.1.
AY693058 Genomic DNA. Translation: AAT93077.1.
BK006947 Genomic DNA. Translation: DAA10244.1.
PIRiS51295.
RefSeqiNP_014082.1. NM_001183155.1.

Genome annotation databases

EnsemblFungiiYNL317W; YNL317W; YNL317W.
GeneIDi855399.
KEGGisce:YNL317W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86378.1 .
Z71593 Genomic DNA. Translation: CAA96247.1 .
AY693058 Genomic DNA. Translation: AAT93077.1 .
BK006947 Genomic DNA. Translation: DAA10244.1 .
PIRi S51295.
RefSeqi NP_014082.1. NM_001183155.1.

3D structure databases

ProteinModelPortali P42841.
SMRi P42841. Positions 47-382.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35522. 33 interactions.
DIPi DIP-5816N.
IntActi P42841. 19 interactions.
MINTi MINT-381244.
STRINGi 4932.YNL317W.

Proteomic databases

MaxQBi P42841.
PaxDbi P42841.
PeptideAtlasi P42841.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL317W ; YNL317W ; YNL317W .
GeneIDi 855399.
KEGGi sce:YNL317W.

Organism-specific databases

CYGDi YNL317w.
SGDi S000005261. PFS2.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00730000111130.
HOGENOMi HOG000213918.
KOi K15542.
OMAi RTIRFWT.
OrthoDBi EOG7DC2DB.

Enzyme and pathway databases

BioCyci YEAST:G3O-33303-MONOMER.

Miscellaneous databases

NextBioi 979220.

Gene expression databases

Genevestigatori P42841.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 4 hits.
[Graphical view ]
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
    Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
    EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 83-91, FUNCTION, IDENTIFICATION IN THE CPF COMPLEX, INTERACTION WITH YSH1; FIP1 AND RNA14.
  6. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
    Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
    J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPFS2_YEAST
AccessioniPrimary (citable) accession number: P42841
Secondary accession number(s): D6W0M8, Q6B1M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7810 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3