ID   YN60_YEAST              Reviewed;         284 AA.
AC   P42840; D6W0M6;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 145.
DE   RecName: Full=Uncharacterized membrane protein YNL320W;
GN   OrderedLocusNames=YNL320W; ORFNames=N0342;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / FY1676;
RX   PubMed=7502583; DOI=10.1002/yea.320111109;
RA   Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.;
RT   "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV
RT   identifies six known genes, a new member of the hexose transporter family
RT   and ten new open reading frames.";
RL   Yeast 11:1077-1085(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=16823961; DOI=10.1021/pr050477f;
RA   Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT   "Toward the complete yeast mitochondrial proteome: multidimensional
RT   separation techniques for mitochondrial proteomics.";
RL   J. Proteome Res. 5:1543-1554(2006).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC       {ECO:0000269|PubMed:16823961}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16823961}.
CC   -!- MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: To S.pombe bem46 and M.tuberculosis Rv2307c. {ECO:0000305}.
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DR   EMBL; Z46259; CAA86377.1; -; Genomic_DNA.
DR   EMBL; Z71596; CAA96251.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10242.1; -; Genomic_DNA.
DR   PIR; S51294; S51294.
DR   RefSeq; NP_014079.1; NM_001183158.1.
DR   AlphaFoldDB; P42840; -.
DR   SMR; P42840; -.
DR   BioGRID; 35520; 22.
DR   DIP; DIP-4158N; -.
DR   IntAct; P42840; 1.
DR   STRING; 4932.YNL320W; -.
DR   ESTHER; yeast-yn60; ABHD13-BEM46.
DR   MEROPS; S09.A35; -.
DR   MaxQB; P42840; -.
DR   PaxDb; 4932-YNL320W; -.
DR   PeptideAtlas; P42840; -.
DR   EnsemblFungi; YNL320W_mRNA; YNL320W; YNL320W.
DR   GeneID; 855396; -.
DR   KEGG; sce:YNL320W; -.
DR   AGR; SGD:S000005264; -.
DR   SGD; S000005264; YNL320W.
DR   VEuPathDB; FungiDB:YNL320W; -.
DR   eggNOG; KOG4391; Eukaryota.
DR   GeneTree; ENSGT00940000158114; -.
DR   HOGENOM; CLU_029375_2_0_1; -.
DR   InParanoid; P42840; -.
DR   OMA; QYWTSED; -.
DR   OrthoDB; 507at2759; -.
DR   BioCyc; YEAST:G3O-33305-MONOMER; -.
DR   BioGRID-ORCS; 855396; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P42840; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P42840; Protein.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0008474; F:palmitoyl-(protein) hydrolase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR022742; Hydrolase_4.
DR   PANTHER; PTHR12277; ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR12277:SF203; PROTEIN ABHD13; 1.
DR   Pfam; PF12146; Hydrolase_4; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..284
FT                   /note="Uncharacterized membrane protein YNL320W"
FT                   /id="PRO_0000203364"
FT   TOPO_DOM        1..8
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..80
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        102..284
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   284 AA;  32308 MW;  933B35169A9CCCDA CRC64;
     MLWKVSKMFL GGLVALTTIS VATLYHYQNR LVYPSWAQGA RNHVDTPDSR GIPYEKLTLI
     TQDHIKLEAW DIKNENSTST VLILCPNAGN IGYFILIIDI FYRQFGMSVF IYSYRGYGNS
     EGSPSEKGLK LDADCVISHL STDSFHSKRK LVLYGRSLGG ANALYIASKF RDLCDGVILE
     NTFLSIRKVI PYIFPLLKRF TLLCHEIWNS EGLMGSCSSE TPFLFLSGLK DEIVPPFHMR
     KLYETCPSSN KKIFEFPLGS HNDTIIQDGY WDIIRDFLIE KGFI
//