P42840 (YN60_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized membrane protein YNL320W

Gene names

Ordered Locus Names:	YNL320W
ORF Names:	N0342

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium.
Sequence similarities	To S.pombe bem46 and M.tuberculosis Rv2307c.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay PubMed 16823961. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Uncharacterized membrane protein YNL320W		PRO_0000203364
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 25	17	Helical; Potential
	Topological domain	26 – 80	55	Extracellular Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Topological domain	102 – 284	183	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P42840 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 933B35169A9CCCDA	FASTA	284	32,308
	10 20 30 40 50 60 MLWKVSKMFL GGLVALTTIS VATLYHYQNR LVYPSWAQGA RNHVDTPDSR GIPYEKLTLI 70 80 90 100 110 120 TQDHIKLEAW DIKNENSTST VLILCPNAGN IGYFILIIDI FYRQFGMSVF IYSYRGYGNS 130 140 150 160 170 180 EGSPSEKGLK LDADCVISHL STDSFHSKRK LVLYGRSLGG ANALYIASKF RDLCDGVILE 190 200 210 220 230 240 NTFLSIRKVI PYIFPLLKRF TLLCHEIWNS EGLMGSCSSE TPFLFLSGLK DEIVPPFHMR 250 260 270 280 KLYETCPSSN KKIFEFPLGS HNDTIIQDGY WDIIRDFLIE KGFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86377.1. Z71596 Genomic DNA. Translation: CAA96251.1. BK006947 Genomic DNA. Translation: DAA10242.1.
PIR	S51294.
RefSeq	NP_014079.1. NM_001183158.1.
3D structure databases
ProteinModelPortal	P42840.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4158N.
IntAct	P42840. 1 interaction.
MINT	MINT-540401.
STRING	4932.YNL320W.
Protein family/group databases
MEROPS	S09.A35.
Proteomic databases
PaxDb	P42840.
PeptideAtlas	P42840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL320W; YNL320W; YNL320W.
GeneID	855396.
KEGG	sce:YNL320W.
Organism-specific databases
CYGD	YNL320w.
SGD	S000005264. YNL320W.
Phylogenomic databases
eggNOG	COG1073.
GeneTree	ENSGT00390000000948.
HOGENOM	HOG000007143.
KO	K06889.
OMA	IRYTGDS.
OrthoDB	EOG4C5GTK.
Gene expression databases
Genevestigator	P42840.
GermOnline	YNL320W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979214.

Entry information

Entry name

YN60_YEAST

Accession

Primary (citable) accession number: P42840
Secondary accession number(s): D6W0M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program