Reviewed, UniProtKB/Swiss-Prot P42840 (YN60_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized membrane protein YNL320W

Gene names

Ordered Locus Names:	YNL320W
ORF Names:	N0342

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	To S.pombe bem46 and M.tuberculosis Rv2307c.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Uncharacterized membrane protein YNL320W		PRO_0000203364
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 25	17	Potential
	Topological domain	26 – 80	55	Extracellular Potential
	Transmembrane	81 – 101	21	Potential
	Topological domain	102 – 284	183	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P42840-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 933B35169A9CCCDA	FASTA	284	32,308
	10 20 30 40 50 60 MLWKVSKMFL GGLVALTTIS VATLYHYQNR LVYPSWAQGA RNHVDTPDSR GIPYEKLTLI 70 80 90 100 110 120 TQDHIKLEAW DIKNENSTST VLILCPNAGN IGYFILIIDI FYRQFGMSVF IYSYRGYGNS 130 140 150 160 170 180 EGSPSEKGLK LDADCVISHL STDSFHSKRK LVLYGRSLGG ANALYIASKF RDLCDGVILE 190 200 210 220 230 240 NTFLSIRKVI PYIFPLLKRF TLLCHEIWNS EGLMGSCSSE TPFLFLSGLK DEIVPPFHMR 250 260 270 280 KLYETCPSSN KKIFEFPLGS HNDTIIQDGY WDIIRDFLIE KGFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86377.1. Z71596 Genomic DNA. Translation: CAA96251.1.
PIR	S51294.
RefSeq	NP_014079.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4158N.
IntAct	P42840. 2 interactions.
STRING	P42840.
Proteomic databases
PeptideAtlas	P42840.
Genome annotation databases
Ensembl	YNL320W; YNL320W; YNL320W; Saccharomyces cerevisiae. [Genome view]
GeneID	855396.
KEGG	sce:YNL320W.
NMPDR	fig|4932.3.peg.5141.
Organism-specific databases
CYGD	YNL320w.
SGD	S000005264. YNL320W.
Phylogenomic databases
HOGENOM	HBG678036.
OMA	EQFIREV.
OrthoDB	EOG95X9CQ.
Gene expression databases
ArrayExpress	P42840.
Genevestigator	P42840.
GermOnline	YNL320W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	979214.

Entry information

Entry name

YN60_YEAST

Accession

Primary (citable) accession number: P42840

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)