Low affinity vacuolar monovalent cation/H(+) antiporter - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P42839 (VNX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Low affinity vacuolar monovalent cation/H(+) antiporter

Alternative name(s):
Vacuolar Na(+)/H(+) exchanger

Gene names

Name:	VNX1
Ordered Locus Names:	YNL321W
ORF Names:	N0339

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in promoting intracellular monovalent cation sequestration via the exchange of monovalent cations and especially Na⁺ for hydrogen ions across the vacuolar membrane. Ref.8
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.8.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family.
Biophysicochemical properties	Kinetic parameters: K_M=22.4 mM for Na⁺ transport Ref.8 K_M=82.2 mM for K⁺ transport

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from direct assay. Source: SGD fungal-type vacuole membrane Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium:hydrogen antiporter activity Inferred from mutant phenotype. Source: SGD potassium:hydrogen antiporter activity Inferred from mutant phenotype Ref.8. Source: SGD sodium:hydrogen antiporter activity Inferred from mutant phenotype Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 908

908

Low affinity vacuolar monovalent cation/H(+) antiporter

PRO_0000209505

Regions

Topological domain

1 – 244

244

Cytoplasmic Potential

Transmembrane

245 – 265

Helical; Potential

Topological domain

266 – 408

143

Vacuolar Potential

Transmembrane

409 – 429

Helical; Potential

Topological domain

430 – 494

Cytoplasmic Potential

Transmembrane

495 – 515

Helical; Potential

Topological domain

516 – 530

Vacuolar Potential

Transmembrane

531 – 551

Helical; Potential

Topological domain

552 – 560

Cytoplasmic Potential

Transmembrane

561 – 581

Helical; Potential

Topological domain

582 – 587

Vacuolar Potential

Transmembrane

588 – 608

Helical; Potential

Topological domain

609 – 626

Cytoplasmic Potential

Transmembrane

627 – 647

Helical; Potential

Topological domain

648 – 686

Vacuolar Potential

Transmembrane

687 – 707

Helical; Potential

Topological domain

708 – 746

Cytoplasmic Potential

Transmembrane

747 – 767

Helical; Potential

Topological domain

768 – 783

Vacuolar Potential

Transmembrane

784 – 804

Helical; Potential

Topological domain

805 – 816

Cytoplasmic Potential

Transmembrane

817 – 837

Helical; Potential

Topological domain

838 – 851

Vacuolar Potential

Transmembrane

852 – 872

Helical; Potential

Topological domain

873 – 885

Cytoplasmic Potential

Transmembrane

886 – 906

Helical; Potential

Topological domain

907 – 908

Vacuolar Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.9 Ref.11

Modified residue

110

Phosphoserine Ref.4 Ref.6 Ref.9 Ref.11

Modified residue

120

Phosphoserine Ref.4 Ref.10 Ref.11

Modified residue

121

Phosphoserine Ref.6 Ref.11

Glycosylation

361

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P42839 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 180E0EEC77DC111E
Show »

FASTA

908

102,499

        10         20         30         40         50         60 
MAKNNHISAS GNSTSGDHRL KEEVLTPTTS ASTPHRIFSV DDDPKEIQND IRYLEGLHEG 

        70         80         90        100        110        120 
LKFALHANKS KRSVSSQSPI VHSSNNTLHH HEHQQHLPPT LESLSSKSHS VPDLNTATPS 

       130        140        150        160        170        180 
SPKRMHSSIR ELPHDDNDDE DANDDSRFII HDSHGHDLLI DEINCQSPSH LENNDQASNA 

       190        200        210        220        230        240 
SSTESFTLRE RQDAINETHP FGIRIWKPAL YKKHRSVQRT AAQDIHETQL KTITWEVTCS 

       250        260        270        280        290        300 
NVLWFILFGF PIAILFYSAA IVVFLLGGGG LVTNSAKEYS KCLYKLANYF LWPFGKMVYL 

       310        320        330        340        350        360 
LQDEQYLQED KDEGISMQQF YNWVTSYSNR LVFHQSQAKF QQREDHPAPA TESSSLMPPA 

       370        380        390        400        410        420 
NTTATPLNSN HPSYNSIRHE IPHAAAQRRY FGRGKWSWGR VLFYTIFHLV LQPILAVLSL 

       430        440        450        460        470        480 
CLWLLVFTIP MSNVLWQIMY HCRRHPLALG FKYVENSSQS HENEITQQQL NKNILLCTFR 

       490        500        510        520        530        540 
AAGWHYYKYT VDGTNVIVVN LISIVFFTIF DFYVLKNFLH WKTWFTYESS IFILCLTSTI 

       550        560        570        580        590        600 
PLAFYIGQAV ASISAQTSMG VGAVINAFFS TIVEIFLYCV ALQQKKGLLV EGSMIGSILG 

       610        620        630        640        650        660 
AVLLLPGLSM CGGALNRKTQ RYNPASAGVS SALLIFSMIV MFVPTVLYEI YGGYSVNCAD 

       670        680        690        700        710        720 
GANDRDCTFS HPPLKFNRLF THVIQPMSIS CAIVLFCAYI IGLWFTLRTH AKMIWQLPIA 

       730        740        750        760        770        780 
DPTSTAPEQQ EQNSHDAPNW SRSKSTCILL MSTLLYAIIA EILVSCVDAV LEDIPSLNPK 

       790        800        810        820        830        840 
FLGLTIFALI PNTTEFLNAI SFAIHGNVAL SMEIGSAYAL QVCLLQIPSL VIYSIFYTWN 

       850        860        870        880        890        900 
VKKSMINIRT QMFPLVFPRW DIFGAMTSVF MFTYLYAEGK SNYFKGSMLI LLYIIIVVGF 


YFQGALSE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110 AND SER-120, MASS SPECTROMETRY. Strain: 2124.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110 AND SER-121, MASS SPECTROMETRY. Strain: SUB592.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"Identification and characterization of Vnx1p, a novel type of vacuolar monovalent cation/H+ antiporter of Saccharomyces cerevisiae." Cagnac O., Leterrier M., Yeager M., Blumwald E. J. Biol. Chem. 282:24284-24293(2007) [PubMed: 17588950] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26 AND SER-110, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; THR-26; SER-110; SER-120 AND SER-121, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86376.1. Z71597 Genomic DNA. Translation: CAA96252.1. BK006947 Genomic DNA. Translation: DAA10241.1.
PIR	S51293.
RefSeq	NP_014078.1. NM_001183159.1.
3D structure databases
ProteinModelPortal	P42839.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4268N.
MINT	MINT-526548.
STRING	P42839.
Protein family/group databases
TCDB	2.A.19.7.1. Ca2+:cation antiporter (CaCA) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL321W; YNL321W; YNL321W.
GeneID	855395.
KEGG	sce:YNL321W.
NMPDR	fig\|4932.3.peg.5140.
Organism-specific databases
CYGD	YNL321w.
SGD	S000005265. VNX1.
Phylogenomic databases
eggNOG	fuNOG05616.
GeneTree	EFGT00050000003767.
HOGENOM	HBG398302.
OMA	EGKSNYF.
OrthoDB	EOG4BP4M6.
Gene expression databases
ArrayExpress	P42839.
Genevestigator	P42839.
GermOnline	YNL321W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005185. DUF307. IPR004837. NaCa_Exmemb. [Graphical view]
KO	K07300.
Pfam	PF03733. DUF307. 1 hit. PF01699. Na_Ca_ex. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	979211.

Entry information

Entry name

VNX1_YEAST

Accession

Primary (citable) accession number: P42839
Secondary accession number(s): D6W0M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents