Reviewed, UniProtKB/Swiss-Prot P42839 (VNX1_YEAST)

Names and origin

Protein names

Recommended name:
    Low affinity vacuolar monovalent cation/H(+) antiporter
Alternative name(s):
    Vacuolar Na(+)/H(+) exchanger

Gene names

Name:	VNX1
Ordered Locus Names:	YNL321W
ORF Names:	N0339

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in promoting intracellular monovalent cation sequestration via the exchange of monovalent cations and especially Na+ for hydrogen ions across the vacuolar membrane. Ref.7
Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family.
Biophysicochemical properties	Kinetic parameters: KM=22.4 mM for Na+ transport KM=82.2 mM for K+ transport

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	potassium ion transport Ref.7 Inferred from mutant phenotype. Source: SGD sodium ion transport Ref.7 Inferred from mutant phenotype. Source: SGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	fungal-type vacuole Ref.7 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium:hydrogen antiporter activity Ref.7 Inferred from mutant phenotype. Source: SGD sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sodium:hydrogen antiporter activity Ref.7 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 908	908	Low affinity vacuolar monovalent cation/H(+) antiporter		PRO_0000209505
Regions
	Topological domain	1 – 244	244	Cytoplasmic Potential
	Transmembrane	245 – 265	21	Potential
	Topological domain	266 – 408	143	Vacuolar Potential
	Transmembrane	409 – 429	21	Potential
	Topological domain	430 – 494	65	Cytoplasmic Potential
	Transmembrane	495 – 515	21	Potential
	Topological domain	516 – 530	15	Vacuolar Potential
	Transmembrane	531 – 551	21	Potential
	Topological domain	552 – 560	9	Cytoplasmic Potential
	Transmembrane	561 – 581	21	Potential
	Topological domain	582 – 587	6	Vacuolar Potential
	Transmembrane	588 – 608	21	Potential
	Topological domain	609 – 626	18	Cytoplasmic Potential
	Transmembrane	627 – 647	21	Potential
	Topological domain	648 – 686	39	Vacuolar Potential
	Transmembrane	687 – 707	21	Potential
	Topological domain	708 – 746	39	Cytoplasmic Potential
	Transmembrane	747 – 767	21	Potential
	Topological domain	768 – 783	16	Vacuolar Potential
	Transmembrane	784 – 804	21	Potential
	Topological domain	805 – 816	12	Cytoplasmic Potential
	Transmembrane	817 – 837	21	Potential
	Topological domain	838 – 851	14	Vacuolar Potential
	Transmembrane	852 – 872	21	Potential
	Topological domain	873 – 885	13	Cytoplasmic Potential
	Transmembrane	886 – 906	21	Potential
	Topological domain	907 – 908	2	Vacuolar Potential
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.10
	Modified residue	26	1	Phosphothreonine Ref.10 Ref.8
	Modified residue	110	1	Phosphoserine Ref.10 Ref.8 Ref.3 Ref.5
	Modified residue	120	1	Phosphoserine Ref.10 Ref.3 Ref.9
	Modified residue	121	1	Phosphoserine Ref.10 Ref.5
	Glycosylation	361	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42839-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 180E0EEC77DC111E	FASTA	908	102,499
	10 20 30 40 50 60 MAKNNHISAS GNSTSGDHRL KEEVLTPTTS ASTPHRIFSV DDDPKEIQND IRYLEGLHEG 70 80 90 100 110 120 LKFALHANKS KRSVSSQSPI VHSSNNTLHH HEHQQHLPPT LESLSSKSHS VPDLNTATPS 130 140 150 160 170 180 SPKRMHSSIR ELPHDDNDDE DANDDSRFII HDSHGHDLLI DEINCQSPSH LENNDQASNA 190 200 210 220 230 240 SSTESFTLRE RQDAINETHP FGIRIWKPAL YKKHRSVQRT AAQDIHETQL KTITWEVTCS 250 260 270 280 290 300 NVLWFILFGF PIAILFYSAA IVVFLLGGGG LVTNSAKEYS KCLYKLANYF LWPFGKMVYL 310 320 330 340 350 360 LQDEQYLQED KDEGISMQQF YNWVTSYSNR LVFHQSQAKF QQREDHPAPA TESSSLMPPA 370 380 390 400 410 420 NTTATPLNSN HPSYNSIRHE IPHAAAQRRY FGRGKWSWGR VLFYTIFHLV LQPILAVLSL 430 440 450 460 470 480 CLWLLVFTIP MSNVLWQIMY HCRRHPLALG FKYVENSSQS HENEITQQQL NKNILLCTFR 490 500 510 520 530 540 AAGWHYYKYT VDGTNVIVVN LISIVFFTIF DFYVLKNFLH WKTWFTYESS IFILCLTSTI 550 560 570 580 590 600 PLAFYIGQAV ASISAQTSMG VGAVINAFFS TIVEIFLYCV ALQQKKGLLV EGSMIGSILG 610 620 630 640 650 660 AVLLLPGLSM CGGALNRKTQ RYNPASAGVS SALLIFSMIV MFVPTVLYEI YGGYSVNCAD 670 680 690 700 710 720 GANDRDCTFS HPPLKFNRLF THVIQPMSIS CAIVLFCAYI IGLWFTLRTH AKMIWQLPIA 730 740 750 760 770 780 DPTSTAPEQQ EQNSHDAPNW SRSKSTCILL MSTLLYAIIA EILVSCVDAV LEDIPSLNPK 790 800 810 820 830 840 FLGLTIFALI PNTTEFLNAI SFAIHGNVAL SMEIGSAYAL QVCLLQIPSL VIYSIFYTWN 850 860 870 880 890 900 VKKSMINIRT QMFPLVFPRW DIFGAMTSVF MFTYLYAEGK SNYFKGSMLI LLYIIIVVGF YFQGALSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110 AND SER-120, MASS SPECTROMETRY.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110 AND SER-121, MASS SPECTROMETRY.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[7]	"Identification and characterization of Vnx1p, a novel type of vacuolar monovalent cation/H+ antiporter of Saccharomyces cerevisiae." Cagnac O., Leterrier M., Yeager M., Blumwald E. J. Biol. Chem. 282:24284-24293(2007) [PubMed: 17588950] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26 AND SER-110, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; THR-26; SER-110; SER-120 AND SER-121, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86376.1. Z71597 Genomic DNA. Translation: CAA96252.1.
PIR	S51293.
RefSeq	NP_014078.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4268N.
IntAct	P42839. 1 interaction.
STRING	P42839.
Protein family/group databases
TCDB	2.A.19.7.1. Ca2+:cation antiporter (CaCA) family.
Genome annotation databases
Ensembl	YNL321W; YNL321W; YNL321W; Saccharomyces cerevisiae. [Genome view]
GeneID	855395.
KEGG	sce:YNL321W.
NMPDR	fig|4932.3.peg.5140.
Organism-specific databases
CYGD	YNL321w.
SGD	S000005265. VNX1.
Phylogenomic databases
HOGENOM	P42839.
OMA	EGKSNYF
OrthoDB	EOG9XPRZ4
Gene expression databases
ArrayExpress	P42839.
Genevestigator	P42839.
GermOnline	YNL321W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005185. DUF307. IPR004837. NaCa_Exmemb. [Graphical view]
Pfam	PF03733. DUF307. 1 hit. PF01699. Na_Ca_ex. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979211.

Entry information

Entry name

VNX1_YEAST

Accession

Primary (citable) accession number: P42839

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)