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P42838

- LEM3_YEAST

UniProt

P42838 - LEM3_YEAST

Protein

Alkylphosphocholine resistance protein LEM3

Gene

LEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.

    GO - Molecular functioni

    1. phospholipid-translocating ATPase activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: SGD
    2. phospholipid translocation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33308-MONOMER.

    Protein family/group databases

    TCDBi8.A.27.1.1. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkylphosphocholine resistance protein LEM3
    Alternative name(s):
    Brefeldin-A sensitivity protein 3
    Ro-sensitive 3
    Gene namesi
    Name:LEM3
    Synonyms:BRE3, ROS3
    Ordered Locus Names:YNL323W
    ORF Names:N0333
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL323w.
    SGDiS000005267. LEM3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. integral component of membrane Source: SGD
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Alkylphosphocholine resistance protein LEM3PRO_0000207671Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine3 Publications
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP42838.
    PaxDbiP42838.
    PeptideAtlasiP42838.

    Expressioni

    Gene expression databases

    GenevestigatoriP42838.

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DNF1P326604EBI-28396,EBI-3121

    Protein-protein interaction databases

    BioGridi35517. 131 interactions.
    DIPiDIP-4598N.
    IntActiP42838. 5 interactions.
    MINTiMINT-549319.
    STRINGi4932.YNL323W.

    Structurei

    3D structure databases

    ProteinModelPortaliP42838.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei75 – 9521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei373 – 39321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CDC50/LEM3 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5035.
    GeneTreeiENSGT00390000004660.
    HOGENOMiHOG000209056.
    OMAiGCICAAM.
    OrthoDBiEOG7M3JB3.

    Family and domain databases

    InterProiIPR005045. DUF284_TM_euk.
    [Graphical view]
    PANTHERiPTHR10926. PTHR10926. 1 hit.
    PfamiPF03381. CDC50. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P42838-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN    50
    RRPKEDAFTQ QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV 100
    DEVTIYYQDC MTNATSSWSD IPSEHWQFVF HKYKTYNTAP QWRFVDDESD 150
    DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE KFAANHRRYV LSFSEDQIRG 200
    EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN DTFPLQLTNV 250
    GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 300
    NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV 350
    LEFNGKKGIY LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG 400
    RKIADASSLS WNMK 414
    Length:414
    Mass (Da):47,438
    Last modified:November 1, 1995 - v1
    Checksum:i55BDA1F32251B4E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86374.1.
    Z71599 Genomic DNA. Translation: CAA96254.1.
    BK006947 Genomic DNA. Translation: DAA10239.1.
    PIRiS55865.
    RefSeqiNP_014076.1. NM_001183161.1.

    Genome annotation databases

    EnsemblFungiiYNL323W; YNL323W; YNL323W.
    GeneIDi855393.
    KEGGisce:YNL323W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86374.1 .
    Z71599 Genomic DNA. Translation: CAA96254.1 .
    BK006947 Genomic DNA. Translation: DAA10239.1 .
    PIRi S55865.
    RefSeqi NP_014076.1. NM_001183161.1.

    3D structure databases

    ProteinModelPortali P42838.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35517. 131 interactions.
    DIPi DIP-4598N.
    IntActi P42838. 5 interactions.
    MINTi MINT-549319.
    STRINGi 4932.YNL323W.

    Protein family/group databases

    TCDBi 8.A.27.1.1. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

    Proteomic databases

    MaxQBi P42838.
    PaxDbi P42838.
    PeptideAtlasi P42838.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL323W ; YNL323W ; YNL323W .
    GeneIDi 855393.
    KEGGi sce:YNL323W.

    Organism-specific databases

    CYGDi YNL323w.
    SGDi S000005267. LEM3.

    Phylogenomic databases

    eggNOGi COG5035.
    GeneTreei ENSGT00390000004660.
    HOGENOMi HOG000209056.
    OMAi GCICAAM.
    OrthoDBi EOG7M3JB3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33308-MONOMER.

    Miscellaneous databases

    NextBioi 979205.

    Gene expression databases

    Genevestigatori P42838.

    Family and domain databases

    InterProi IPR005045. DUF284_TM_euk.
    [Graphical view ]
    PANTHERi PTHR10926. PTHR10926. 1 hit.
    Pfami PF03381. CDC50. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015840. DUF284_TM_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae."
      Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M.
      J. Biol. Chem. 277:37855-37862(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine."
      Hanson P.K., Malone L., Birchmore J.L., Nichols J.W.
      J. Biol. Chem. 278:36041-36050(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLEM3_YEAST
    AccessioniPrimary (citable) accession number: P42838
    Secondary accession number(s): D6W0M3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2460 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3