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Protein

Alkylphosphocholine resistance protein LEM3

Gene

LEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.

Miscellaneous

Present with 2460 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  • cell surface receptor signaling pathway Source: SGD
  • phospholipid translocation Source: SGD
  • regulation of vacuole organization Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-33308-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation

Protein family/group databases

TCDBi8.A.27.1.1 the cdc50 p-type atpase lipid flippase subunit (cdc50) family

Names & Taxonomyi

Protein namesi
Recommended name:
Alkylphosphocholine resistance protein LEM3
Alternative name(s):
Brefeldin-A sensitivity protein 3
Ro-sensitive 3
Gene namesi
Name:LEM3
Synonyms:BRE3, ROS3
Ordered Locus Names:YNL323W
ORF Names:N0333
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL323W
SGDiS000005267 LEM3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076711 – 414Alkylphosphocholine resistance protein LEM3Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi298N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP42838
PaxDbiP42838
PRIDEiP42838

PTM databases

iPTMnetiP42838

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35517, 240 interactors
DIPiDIP-4598N
IntActiP42838, 17 interactors
MINTiP42838
STRINGi4932.YNL323W

Structurei

3D structure databases

ProteinModelPortaliP42838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC50/LEM3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004660
HOGENOMiHOG000209056
InParanoidiP42838
OMAiPLFFCAG
OrthoDBiEOG092C2TU8

Family and domain databases

InterProiView protein in InterPro
IPR005045 CDC50/LEM3_fam
PANTHERiPTHR10926 PTHR10926, 1 hit
PfamiView protein in Pfam
PF03381 CDC50, 1 hit
PIRSFiPIRSF015840 DUF284_TM_euk, 1 hit

Sequencei

Sequence statusi: Complete.

P42838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN
60 70 80 90 100
RRPKEDAFTQ QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV
110 120 130 140 150
DEVTIYYQDC MTNATSSWSD IPSEHWQFVF HKYKTYNTAP QWRFVDDESD
160 170 180 190 200
DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE KFAANHRRYV LSFSEDQIRG
210 220 230 240 250
EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN DTFPLQLTNV
260 270 280 290 300
GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET
310 320 330 340 350
NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV
360 370 380 390 400
LEFNGKKGIY LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG
410
RKIADASSLS WNMK
Length:414
Mass (Da):47,438
Last modified:November 1, 1995 - v1
Checksum:i55BDA1F32251B4E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA Translation: CAA86374.1
Z71599 Genomic DNA Translation: CAA96254.1
BK006947 Genomic DNA Translation: DAA10239.1
PIRiS55865
RefSeqiNP_014076.1, NM_001183161.1

Genome annotation databases

EnsemblFungiiYNL323W; YNL323W; YNL323W
GeneIDi855393
KEGGisce:YNL323W

Similar proteinsi

Entry informationi

Entry nameiLEM3_YEAST
AccessioniPrimary (citable) accession number: P42838
Secondary accession number(s): D6W0M3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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