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P42838 (LEM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkylphosphocholine resistance protein LEM3
Alternative name(s):
Brefeldin-A sensitivity protein 3
Ro-sensitive 3
Gene names
Name:LEM3
Synonyms:BRE3, ROS3
Ordered Locus Names:YNL323W
ORF Names:N0333
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.

Subunit structure

Monomer.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Miscellaneous

Present with 2460 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CDC50/LEM3 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DNF1P326604EBI-28396,EBI-3121

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Alkylphosphocholine resistance protein LEM3
PRO_0000207671

Regions

Transmembrane75 – 9521Helical; Potential
Transmembrane373 – 39321Helical; Potential

Amino acid modifications

Modified residue361Phosphoserine Ref.7 Ref.8 Ref.9
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation2791N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P42838 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 55BDA1F32251B4E6

FASTA41447,438
        10         20         30         40         50         60 
MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ 

        70         80         90        100        110        120 
QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD 

       130        140        150        160        170        180 
IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE 

       190        200        210        220        230        240 
KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN 

       250        260        270        280        290        300 
DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 

       310        320        330        340        350        360 
NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY 

       370        380        390        400        410 
LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae."
Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M.
J. Biol. Chem. 277:37855-37862(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine."
Hanson P.K., Malone L., Birchmore J.L., Nichols J.W.
J. Biol. Chem. 278:36041-36050(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46259 Genomic DNA. Translation: CAA86374.1.
Z71599 Genomic DNA. Translation: CAA96254.1.
BK006947 Genomic DNA. Translation: DAA10239.1.
PIRS55865.
RefSeqNP_014076.1. NM_001183161.1.

3D structure databases

ProteinModelPortalP42838.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35517. 129 interactions.
DIPDIP-4598N.
IntActP42838. 5 interactions.
MINTMINT-549319.
STRING4932.YNL323W.

Protein family/group databases

TCDB8.A.27.1.1. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Proteomic databases

MaxQBP42838.
PaxDbP42838.
PeptideAtlasP42838.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL323W; YNL323W; YNL323W.
GeneID855393.
KEGGsce:YNL323W.

Organism-specific databases

CYGDYNL323w.
SGDS000005267. LEM3.

Phylogenomic databases

eggNOGCOG5035.
GeneTreeENSGT00390000004660.
HOGENOMHOG000209056.
OMAGCICAAM.
OrthoDBEOG7M3JB3.

Enzyme and pathway databases

BioCycYEAST:G3O-33308-MONOMER.

Gene expression databases

GenevestigatorP42838.

Family and domain databases

InterProIPR005045. DUF284_TM_euk.
[Graphical view]
PANTHERPTHR10926. PTHR10926. 1 hit.
PfamPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFPIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNetSearch...

Other

NextBio979205.

Entry information

Entry nameLEM3_YEAST
AccessionPrimary (citable) accession number: P42838
Secondary accession number(s): D6W0M3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families