Reviewed,
UniProtKB/Swiss-Prot P42838 (LEM3_YEAST)
Last modified
June 16, 2009.
Version 75.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alkylphosphocholine resistance protein LEM3 Alternative name(s): Ro-sensitive 3 Brefeldin-A sensitivity protein 3 | ||||||||
| Gene names |
| ||||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 414 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane. |
| Subunit structure | Monomer. |
| Subcellular location | |
| Miscellaneous | Present with 2460 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the CDC50/LEM3 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane |
| PTM | Glycoprotein Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell surface receptor linked signal transduction Inferred from mutant phenotype. Source: SGD phospholipid translocation Ref.4Inferred from mutant phenotype. Source: SGD |
| Cellular component | endoplasmic reticulum Ref.3 Inferred from direct assay. Source: SGD integral to membraneInferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.3Inferred from direct assay. Source: SGD |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ADH1 | P00330 | 1 | EBI-28396,EBI-2218 | |
| AGP1 | P25376 | 1 | EBI-28396,EBI-2357 | |
| ARF1 | P11076 | 1 | EBI-28396,EBI-2816 | |
| BSC6 | Q08280 | 1 | EBI-28396,EBI-35858 | |
| DNF1 | P32660 | 2 | EBI-28396,EBI-3121 | |
| DNF2 | Q12675 | 1 | EBI-28396,EBI-3114 | |
| GAP1 | P19145 | 1 | EBI-28396,EBI-7314 | |
| PMP2 | P40975 | 1 | EBI-28396,EBI-2043041 | |
| PMP3 | P87284 | 1 | EBI-28396,EBI-13555 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 414 | 414 | Alkylphosphocholine resistance protein LEM3 | PRO_0000207671 | |||||
Regions | |||||||||
| Transmembrane | 75 – 95 | 21 | Potential | ||||||
| Transmembrane | 373 – 393 | 21 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 36 | 1 | Phosphoserine Ref.6 Ref.7 Ref.8 | ||||||
| Glycosylation | 113 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 240 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 256 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 279 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 298 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 332 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae." Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M. J. Biol. Chem. 277:37855-37862(2002) [PubMed: 12133835] [Abstract] Cited for: CHARACTERIZATION. |
| [4] | "Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine." Hanson P.K., Malone L., Birchmore J.L., Nichols J.W. J. Biol. Chem. 278:36041-36050(2003) [PubMed: 12842877] [Abstract] Cited for: CHARACTERIZATION. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. |
| [7] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. |
| [8] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z46259 Genomic DNA. Translation: CAA86374.1. Z71599 Genomic DNA. Translation: CAA96254.1. | |
| PIR | S55865. |
| RefSeq | NP_014076.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:4598N. |
| IntAct | P42838. 28 interactions. |
Protein family/group databases | |
| TCDB | 8.A.27.1.1. phospholipid importer beta-subunit (PLI-beta) family. |
Proteomic databases | |
| PeptideAtlas | P42838. |
Genome annotation databases | |
| Ensembl | YNL323W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 855393. |
| GenomeReviews | Gene locus YNL323W in contig Y13139_GR. |
| KEGG | sce:YNL323W. |
| NMPDR | fig|4932.3.peg.5138. |
Organism-specific databases | |
| CYGD | YNL323w. |
| SGD | S000005267. LEM3. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P42838. |
| OMA | P42838. DVDASEF. |
Gene expression databases | |
| GermOnline | YNL323W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR005045. DUF284_TM_euk. [Graphical view] |
| PANTHER | PTHR10926. DUF284_TM_euk. 1 hit. |
| Pfam | PF03381. CDC50. 1 hit. [Graphical view] |
| PIRSF | PIRSF015840. DUF284_TM_euk. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 979205. |
Entry information
| Entry name | LEM3_YEAST | ||||||||
| Accession | Primary (citable) accession number: P42838 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with


