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P42838

- LEM3_YEAST

UniProt

P42838 - LEM3_YEAST

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Protein

Alkylphosphocholine resistance protein LEM3

Gene
LEM3, BRE3, ROS3, YNL323W, N0333
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.

GO - Molecular functioni

  1. phospholipid-translocating ATPase activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. cell surface receptor signaling pathway Source: SGD
  2. phospholipid translocation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33308-MONOMER.

Protein family/group databases

TCDBi8.A.27.1.1. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkylphosphocholine resistance protein LEM3
Alternative name(s):
Brefeldin-A sensitivity protein 3
Ro-sensitive 3
Gene namesi
Name:LEM3
Synonyms:BRE3, ROS3
Ordered Locus Names:YNL323W
ORF Names:N0333
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL323w.
SGDiS000005267. LEM3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei75 – 9521Helical; Reviewed predictionAdd
BLAST
Transmembranei373 – 39321Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Alkylphosphocholine resistance protein LEM3PRO_0000207671Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphoserine3 Publications
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi240 – 2401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi256 – 2561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi279 – 2791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi298 – 2981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP42838.
PaxDbiP42838.
PeptideAtlasiP42838.

Expressioni

Gene expression databases

GenevestigatoriP42838.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
DNF1P326604EBI-28396,EBI-3121

Protein-protein interaction databases

BioGridi35517. 131 interactions.
DIPiDIP-4598N.
IntActiP42838. 5 interactions.
MINTiMINT-549319.
STRINGi4932.YNL323W.

Structurei

3D structure databases

ProteinModelPortaliP42838.

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC50/LEM3 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5035.
GeneTreeiENSGT00390000004660.
HOGENOMiHOG000209056.
OMAiGCICAAM.
OrthoDBiEOG7M3JB3.

Family and domain databases

InterProiIPR005045. DUF284_TM_euk.
[Graphical view]
PANTHERiPTHR10926. PTHR10926. 1 hit.
PfamiPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P42838-1 [UniParc]FASTAAdd to Basket

« Hide

MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN    50
RRPKEDAFTQ QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV 100
DEVTIYYQDC MTNATSSWSD IPSEHWQFVF HKYKTYNTAP QWRFVDDESD 150
DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE KFAANHRRYV LSFSEDQIRG 200
EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN DTFPLQLTNV 250
GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 300
NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV 350
LEFNGKKGIY LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG 400
RKIADASSLS WNMK 414
Length:414
Mass (Da):47,438
Last modified:November 1, 1995 - v1
Checksum:i55BDA1F32251B4E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86374.1.
Z71599 Genomic DNA. Translation: CAA96254.1.
BK006947 Genomic DNA. Translation: DAA10239.1.
PIRiS55865.
RefSeqiNP_014076.1. NM_001183161.1.

Genome annotation databases

EnsemblFungiiYNL323W; YNL323W; YNL323W.
GeneIDi855393.
KEGGisce:YNL323W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86374.1 .
Z71599 Genomic DNA. Translation: CAA96254.1 .
BK006947 Genomic DNA. Translation: DAA10239.1 .
PIRi S55865.
RefSeqi NP_014076.1. NM_001183161.1.

3D structure databases

ProteinModelPortali P42838.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35517. 131 interactions.
DIPi DIP-4598N.
IntActi P42838. 5 interactions.
MINTi MINT-549319.
STRINGi 4932.YNL323W.

Protein family/group databases

TCDBi 8.A.27.1.1. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Proteomic databases

MaxQBi P42838.
PaxDbi P42838.
PeptideAtlasi P42838.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL323W ; YNL323W ; YNL323W .
GeneIDi 855393.
KEGGi sce:YNL323W.

Organism-specific databases

CYGDi YNL323w.
SGDi S000005267. LEM3.

Phylogenomic databases

eggNOGi COG5035.
GeneTreei ENSGT00390000004660.
HOGENOMi HOG000209056.
OMAi GCICAAM.
OrthoDBi EOG7M3JB3.

Enzyme and pathway databases

BioCyci YEAST:G3O-33308-MONOMER.

Miscellaneous databases

NextBioi 979205.

Gene expression databases

Genevestigatori P42838.

Family and domain databases

InterProi IPR005045. DUF284_TM_euk.
[Graphical view ]
PANTHERi PTHR10926. PTHR10926. 1 hit.
Pfami PF03381. CDC50. 1 hit.
[Graphical view ]
PIRSFi PIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae."
    Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M.
    J. Biol. Chem. 277:37855-37862(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine."
    Hanson P.K., Malone L., Birchmore J.L., Nichols J.W.
    J. Biol. Chem. 278:36041-36050(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLEM3_YEAST
AccessioniPrimary (citable) accession number: P42838
Secondary accession number(s): D6W0M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2460 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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