Reviewed, UniProtKB/Swiss-Prot P42838 (LEM3_YEAST)

Names and origin

Protein names

Recommended name:
    Alkylphosphocholine resistance protein LEM3
Alternative name(s):
    Ro-sensitive 3
    Brefeldin-A sensitivity protein 3

Gene names

Name:	LEM3
Synonyms:	BRE3, ROS3
Ordered Locus Names:	YNL323W
ORF Names:	N0333

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.
Subunit structure	Monomer.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2460 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the CDC50/LEM3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Inferred from mutant phenotype. Source: SGD phospholipid translocation Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum Ref.3 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.3 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADH1	P00330	1	EBI-28396,EBI-2218
AGP1	P25376	1	EBI-28396,EBI-2357
ARF1	P11076	1	EBI-28396,EBI-2816
BSC6	Q08280	1	EBI-28396,EBI-35858
DNF1	P32660	2	EBI-28396,EBI-3121
DNF2	Q12675	1	EBI-28396,EBI-3114
GAP1	P19145	1	EBI-28396,EBI-7314
PMP2	P40975	1	EBI-28396,EBI-2043041
PMP3	P87284	1	EBI-28396,EBI-13555

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Alkylphosphocholine resistance protein LEM3		PRO_0000207671
Regions
	Transmembrane	75 – 95	21	Potential
	Transmembrane	373 – 393	21	Potential
Amino acid modifications
	Modified residue	36	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	332	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42838-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 55BDA1F32251B4E6	FASTA	414	47,438
	10 20 30 40 50 60 MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ 70 80 90 100 110 120 QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD 130 140 150 160 170 180 IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE 190 200 210 220 230 240 KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN 250 260 270 280 290 300 DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 310 320 330 340 350 360 NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY 370 380 390 400 410 LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae." Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M. J. Biol. Chem. 277:37855-37862(2002) [PubMed: 12133835] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine." Hanson P.K., Malone L., Birchmore J.L., Nichols J.W. J. Biol. Chem. 278:36041-36050(2003) [PubMed: 12842877] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86374.1. Z71599 Genomic DNA. Translation: CAA96254.1.
PIR	S55865.
RefSeq	NP_014076.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4598N.
IntAct	P42838. 28 interactions.
Protein family/group databases
TCDB	8.A.27.1.1. phospholipid importer beta-subunit (PLI-beta) family.
Proteomic databases
PeptideAtlas	P42838.
Genome annotation databases
Ensembl	YNL323W. Saccharomyces cerevisiae. [Contig view]
GeneID	855393.
GenomeReviews	Gene locus YNL323W in contig Y13139_GR.
KEGG	sce:YNL323W.
NMPDR	fig|4932.3.peg.5138.
Organism-specific databases
CYGD	YNL323w.
SGD	S000005267. LEM3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P42838.
OMA	P42838. DVDASEF.
Gene expression databases
GermOnline	YNL323W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005045. DUF284_TM_euk. [Graphical view]
PANTHER	PTHR10926. DUF284_TM_euk. 1 hit.
Pfam	PF03381. CDC50. 1 hit. [Graphical view]
PIRSF	PIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	979205.

Entry information

Entry name

LEM3_YEAST

Accession

Primary (citable) accession number: P42838

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)