P42838 (LEM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkylphosphocholine resistance protein LEM3

Alternative name(s):
Brefeldin-A sensitivity protein 3
Ro-sensitive 3

Gene names

Name:	LEM3
Synonyms:	BRE3, ROS3
Ordered Locus Names:	YNL323W
ORF Names:	N0333

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane.
Subunit structure	Monomer.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2460 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CDC50/LEM3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell surface receptor signaling pathway Inferred from mutant phenotype PubMed 11063677. Source: SGD phospholipid translocation Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from sequence or structural similarity PubMed 11063677. Source: SGD plasma membrane Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DNF1	P32660	4	EBI-28396,EBI-3121

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Alkylphosphocholine resistance protein LEM3		PRO_0000207671
Regions
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
Amino acid modifications
	Modified residue	36	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	332	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42838 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 55BDA1F32251B4E6	FASTA	414	47,438
	10 20 30 40 50 60 MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ 70 80 90 100 110 120 QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD 130 140 150 160 170 180 IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE 190 200 210 220 230 240 KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN 250 260 270 280 290 300 DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET 310 320 330 340 350 360 NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY 370 380 390 400 410 LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae." Kato U., Emoto K., Fredriksson C., Nakamura H., Ohta A., Kobayashi T., Murakami-Murofushi K., Kobayashi T., Umeda M. J. Biol. Chem. 277:37855-37862(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine." Hanson P.K., Malone L., Birchmore J.L., Nichols J.W. J. Biol. Chem. 278:36041-36050(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86374.1. Z71599 Genomic DNA. Translation: CAA96254.1. BK006947 Genomic DNA. Translation: DAA10239.1.
PIR	S55865.
RefSeq	NP_014076.1. NM_001183161.1.
3D structure databases
ProteinModelPortal	P42838.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4598N.
IntAct	P42838. 12 interactions.
MINT	MINT-549319.
STRING	4932.YNL323W.
Protein family/group databases
TCDB	8.A.27.1.1. phospholipid importer beta,-subunit (PLI-beta,) family.
Proteomic databases
PaxDb	P42838.
PeptideAtlas	P42838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL323W; YNL323W; YNL323W.
GeneID	855393.
KEGG	sce:YNL323W.
Organism-specific databases
CYGD	YNL323w.
SGD	S000005267. LEM3.
Phylogenomic databases
eggNOG	COG5035.
GeneTree	ENSGT00390000004660.
HOGENOM	HOG000209056.
OMA	SIDKTCT.
OrthoDB	EOG45HW66.
Gene expression databases
Genevestigator	P42838.
GermOnline	YNL323W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005045. DUF284_TM_euk. [Graphical view]
PANTHER	PTHR10926. PTHR10926. 1 hit.
Pfam	PF03381. CDC50. 1 hit. [Graphical view]
PIRSF	PIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNet	Search...
Other
NextBio	979205.

Entry information

Entry name

LEM3_YEAST

Accession

Primary (citable) accession number: P42838
Secondary accession number(s): D6W0M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program