Reviewed, UniProtKB/Swiss-Prot P42836 (PFA3_YEAST)

Names and origin

Protein names

Recommended name:
    Palmitoyltransferase PFA3
    EC=2.3.1.-
Alternative name(s):
    Protein fatty acyltransferase 3

Gene names

Name:	PFA3
Ordered Locus Names:	YNL326C
ORF Names:	N0325

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization. Ref.5
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.5 Ref.3.
Domain	The DHHC domain is required for palmitoyltransferase activity.
Post-translational modification	Autopalmitoylated.
Miscellaneous	Present with 2133 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. PFA3 subfamily. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid palmitoylation Ref.5 Inferred from direct assay. Source: SGD vacuole fusion, non-autophagic Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	fungal-type vacuole membrane Ref.5 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-cysteine S-palmitoleyltransferase activity Ref.5 Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Palmitoyltransferase PFA3		PRO_0000212959
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Potential
	Topological domain	30 – 37	8	Vacuolar Potential
	Transmembrane	38 – 58	21	Potential
	Topological domain	59 – 147	89	Cytoplasmic Potential
	Transmembrane	148 – 168	21	Potential
	Topological domain	169 – 188	20	Vacuolar Potential
	Transmembrane	189 – 209	21	Potential
	Topological domain	210 – 336	127	Cytoplasmic Potential
	Zinc finger	104 – 154	51	DHHC-type
Experimental info
	Mutagenesis	134	1	C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P42836-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D36BF37DEC5D3983	FASTA	336	39,184
	10 20 30 40 50 60 MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA LYTYYKVIAR 70 80 90 100 110 120 GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD GRFRVCQVCH VWKPDRCHHC 130 140 150 160 170 180 SSCDVCILKM DHHCPWFAEC TGFRNQKFFI QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF 190 200 210 220 230 240 NRELIDFHLL GVALLAVAVF ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY 250 260 270 280 290 300 GTNEHLENIF DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ 310 320 330 DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p." Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E. J. Cell Biol. 170:1091-1099(2005) [PubMed: 16186255] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86372.1. Z71602 Genomic DNA. Translation: CAA96258.1.
PIR	S55863.
RefSeq	NP_014073.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4833N.
IntAct	P42836. 3 interactions.
STRING	P42836.
Genome annotation databases
Ensembl	YNL326C; YNL326C; YNL326C; Saccharomyces cerevisiae. [Genome view]
GeneID	855390.
KEGG	sce:YNL326C.
NMPDR	fig|4932.3.peg.5135.
Organism-specific databases
CYGD	YNL326c.
SGD	S000005270. PFA3.
Phylogenomic databases
HOGENOM	HBG396839.
OMA	FPRCLTT.
OrthoDB	EOG9QZ937.
Gene expression databases
ArrayExpress	P42836.
Genevestigator	P42836.
GermOnline	YNL326C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001594. Znf_DHHC. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979199.

Entry information

Entry name

PFA3_YEAST

Accession

Primary (citable) accession number: P42836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)