P42836 (PFA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase PFA3
EC=2.3.1.225

Alternative name(s):
Protein fatty acyltransferase 3

Gene names

Name:	PFA3
Ordered Locus Names:	YNL326C
ORF Names:	N0325

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization. Ref.6
Catalytic activity	Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4 Ref.6.
Domain	The DHHC domain is required for palmitoyltransferase activity.
Post-translational modification	Autopalmitoylated.
Miscellaneous	Present with 2133 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. PFA3 subfamily. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein palmitoylation Inferred from direct assay Ref.6. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype Ref.6PubMed 16301533. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from direct assay Ref.6. Source: SGD integral component of membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	palmitoyltransferase activity Inferred from direct assay Ref.6. Source: SGD protein-cysteine S-palmitoyltransferase activity Inferred from electronic annotation. Source: UniProtKB-EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Palmitoyltransferase PFA3		PRO_0000212959
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Helical; Potential
	Topological domain	30 – 37	8	Vacuolar Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Topological domain	59 – 147	89	Cytoplasmic Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Topological domain	169 – 188	20	Vacuolar Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Topological domain	210 – 336	127	Cytoplasmic Potential
	Zinc finger	104 – 154	51	DHHC-type
Experimental info
	Mutagenesis	134	1	C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P42836 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D36BF37DEC5D3983	FASTA	336	39,184
	10 20 30 40 50 60 MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA LYTYYKVIAR 70 80 90 100 110 120 GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD GRFRVCQVCH VWKPDRCHHC 130 140 150 160 170 180 SSCDVCILKM DHHCPWFAEC TGFRNQKFFI QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF 190 200 210 220 230 240 NRELIDFHLL GVALLAVAVF ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY 250 260 270 280 290 300 GTNEHLENIF DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ 310 320 330 DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p." Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E. J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86372.1. Z71602 Genomic DNA. Translation: CAA96258.1. BK006947 Genomic DNA. Translation: DAA10237.1.
PIR	S55863.
RefSeq	NP_014073.1. NM_001183164.1.
3D structure databases
ProteinModelPortal	P42836.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35515. 8 interactions.
DIP	DIP-4833N.
IntAct	P42836. 2 interactions.
MINT	MINT-479882.
STRING	4932.YNL326C.
Proteomic databases
PaxDb	P42836.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL326C; YNL326C; YNL326C.
GeneID	855390.
KEGG	sce:YNL326C.
Organism-specific databases
CYGD	YNL326c.
SGD	S000005270. PFA3.
Phylogenomic databases
eggNOG	COG5273.
GeneTree	ENSGT00670000097758.
HOGENOM	HOG000246777.
OMA	HYCPWFS.
OrthoDB	EOG71P2MN.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33310-MONOMER.
Gene expression databases
Genevestigator	P42836.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979199.

Entry information

Entry name

PFA3_YEAST

Accession

Primary (citable) accession number: P42836
Secondary accession number(s): D6W0M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program