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P42836 (PFA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Palmitoyltransferase PFA3

EC=2.3.1.225
Alternative name(s):
Protein fatty acyltransferase 3
Gene names
Name:PFA3
Ordered Locus Names:YNL326C
ORF Names:N0325
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization. Ref.6

Catalytic activity

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.4 Ref.6.

Domain

The DHHC domain is required for palmitoyltransferase activity.

Post-translational modification

Autopalmitoylated.

Miscellaneous

Present with 2133 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DHHC palmitoyltransferase family. PFA3 subfamily.

Contains 1 DHHC-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Palmitoyltransferase PFA3
PRO_0000212959

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2923Helical; Potential
Topological domain30 – 378Vacuolar Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 14789Cytoplasmic Potential
Transmembrane148 – 16821Helical; Potential
Topological domain169 – 18820Vacuolar Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 336127Cytoplasmic Potential
Zinc finger104 – 15451DHHC-type

Experimental info

Mutagenesis1341C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P42836 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D36BF37DEC5D3983

FASTA33639,184
        10         20         30         40         50         60 
MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA LYTYYKVIAR 

        70         80         90        100        110        120 
GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD GRFRVCQVCH VWKPDRCHHC 

       130        140        150        160        170        180 
SSCDVCILKM DHHCPWFAEC TGFRNQKFFI QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF 

       190        200        210        220        230        240 
NRELIDFHLL GVALLAVAVF ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY 

       250        260        270        280        290        300 
GTNEHLENIF DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ 

       310        320        330 
DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46259 Genomic DNA. Translation: CAA86372.1.
Z71602 Genomic DNA. Translation: CAA96258.1.
BK006947 Genomic DNA. Translation: DAA10237.1.
PIRS55863.
RefSeqNP_014073.1. NM_001183164.1.

3D structure databases

ProteinModelPortalP42836.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35515. 8 interactions.
DIPDIP-4833N.
IntActP42836. 2 interactions.
MINTMINT-479882.
STRING4932.YNL326C.

Proteomic databases

PaxDbP42836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL326C; YNL326C; YNL326C.
GeneID855390.
KEGGsce:YNL326C.

Organism-specific databases

CYGDYNL326c.
SGDS000005270. PFA3.

Phylogenomic databases

eggNOGCOG5273.
GeneTreeENSGT00750000117306.
HOGENOMHOG000246777.
OMAHYCPWFS.
OrthoDBEOG71P2MN.

Enzyme and pathway databases

BioCycYEAST:G3O-33310-MONOMER.

Gene expression databases

GenevestigatorP42836.

Family and domain databases

InterProIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEPS50216. ZF_DHHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979199.

Entry information

Entry namePFA3_YEAST
AccessionPrimary (citable) accession number: P42836
Secondary accession number(s): D6W0M1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families