Palmitoyltransferase PFA3 - PFA3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P42836 (PFA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Palmitoyltransferase PFA3
EC=2.3.1.225

Alternative name(s):
Protein fatty acyltransferase 3

Gene names

Name:	PFA3
Ordered Locus Names:	YNL326C
ORF Names:	N0325

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization. Ref.6
Catalytic activity	Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4 Ref.6.
Domain	The DHHC domain is required for palmitoyltransferase activity.
Post-translational modification	Autopalmitoylated.
Miscellaneous	Present with 2133 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. PFA3 subfamily. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein palmitoylation Inferred from direct assay Ref.6. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype Ref.6 PubMed 16301533. Source: SGD
Cellular_component	fungal-type vacuole Inferred from direct assay PubMed 16301533. Source: SGD fungal-type vacuole membrane Inferred from direct assay Ref.6. Source: SGD integral component of membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from direct assay PubMed 16647879. Source: UniProt
Molecular_function	palmitoyltransferase activity Inferred from direct assay Ref.6. Source: SGD protein-cysteine S-palmitoyltransferase activity Inferred from electronic annotation. Source: UniProtKB-EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 336

336

Palmitoyltransferase PFA3

PRO_0000212959

Regions

Topological domain

1 – 6

Cytoplasmic Potential

Transmembrane

7 – 29

Helical; Potential

Topological domain

30 – 37

Vacuolar Potential

Transmembrane

38 – 58

Helical; Potential

Topological domain

59 – 147

Cytoplasmic Potential

Transmembrane

148 – 168

Helical; Potential

Topological domain

169 – 188

Vacuolar Potential

Transmembrane

189 – 209

Helical; Potential

Topological domain

210 – 336

127

Cytoplasmic Potential

Zinc finger

104 – 154

DHHC-type

Experimental info

Mutagenesis

134

C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P42836 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D36BF37DEC5D3983
Show »

FASTA

336

39,184

        10         20         30         40         50         60 
MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA LYTYYKVIAR 

        70         80         90        100        110        120 
GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD GRFRVCQVCH VWKPDRCHHC 

       130        140        150        160        170        180 
SSCDVCILKM DHHCPWFAEC TGFRNQKFFI QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF 

       190        200        210        220        230        240 
NRELIDFHLL GVALLAVAVF ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY 

       250        260        270        280        290        300 
GTNEHLENIF DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ 

       310        320        330 
DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p." Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E. J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86372.1. Z71602 Genomic DNA. Translation: CAA96258.1. BK006947 Genomic DNA. Translation: DAA10237.1.
PIR	S55863.
RefSeq	NP_014073.1. NM_001183164.1.
3D structure databases
ProteinModelPortal	P42836.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35515. 8 interactions.
DIP	DIP-4833N.
IntAct	P42836. 2 interactions.
MINT	MINT-479882.
STRING	4932.YNL326C.
Proteomic databases
PaxDb	P42836.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL326C; YNL326C; YNL326C.
GeneID	855390.
KEGG	sce:YNL326C.
Organism-specific databases
CYGD	YNL326c.
SGD	S000005270. PFA3.
Phylogenomic databases
eggNOG	COG5273.
GeneTree	ENSGT00750000117306.
HOGENOM	HOG000246777.
OMA	HYCPWFS.
OrthoDB	EOG71P2MN.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33310-MONOMER.
Gene expression databases
Genevestigator	P42836.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979199.

Entry information

Entry name

PFA3_YEAST

Accession

Primary (citable) accession number: P42836
Secondary accession number(s): D6W0M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents