UniProtKB - P42836 (PFA3_YEAST)
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Protein
Palmitoyltransferase PFA3
Gene
PFA3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.1 Publication
Miscellaneous
Present with 2133 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.
GO - Molecular functioni
- palmitoyltransferase activity Source: UniProtKB
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- protein palmitoylation Source: UniProtKB
- vacuole fusion, non-autophagic Source: SGD
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | YEAST:G3O-33310-MONOMER. |
Reactomei | R-SCE-203615. eNOS activation. |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase PFA3 (EC:2.3.1.225)Alternative name(s): Protein fatty acyltransferase 3 |
Gene namesi | Name:PFA3 Ordered Locus Names:YNL326C ORF Names:N0325 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL326C. |
SGDi | S000005270. PFA3. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 29 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 30 – 37 | VacuolarSequence analysis | 8 | |
Transmembranei | 38 – 58 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 59 – 147 | CytoplasmicSequence analysisAdd BLAST | 89 | |
Transmembranei | 148 – 168 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 169 – 188 | VacuolarSequence analysisAdd BLAST | 20 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 210 – 336 | CytoplasmicSequence analysisAdd BLAST | 127 |
Keywords - Cellular componenti
Membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 134 | C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212959 | 1 – 336 | Palmitoyltransferase PFA3Add BLAST | 336 |
Post-translational modificationi
Autopalmitoylated.
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P42836. |
PRIDEi | P42836. |
PTM databases
iPTMneti | P42836. |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
CREB3L1 | Q96BA8 | 3 | EBI-28414,EBI-6942903 | From Homo sapiens. |
Protein-protein interaction databases
BioGridi | 35515. 10 interactors. |
DIPi | DIP-4833N. |
IntActi | P42836. 7 interactors. |
MINTi | P42836. |
STRINGi | 4932.YNL326C. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 154 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00880000137864. |
HOGENOMi | HOG000246777. |
InParanoidi | P42836. |
KOi | K20028. |
OMAi | HHCPWFA. |
OrthoDBi | EOG092C524D. |
Family and domain databases
InterProi | View protein in InterPro IPR001594. Palmitoyltrfase_DHHC. |
Pfami | View protein in Pfam PF01529. DHHC. 1 hit. |
PROSITEi | View protein in PROSITE PS50216. DHHC. 1 hit. |
i Sequence
Sequence statusi: Complete.
P42836-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA
60 70 80 90 100
LYTYYKVIAR GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD
110 120 130 140 150
GRFRVCQVCH VWKPDRCHHC SSCDVCILKM DHHCPWFAEC TGFRNQKFFI
160 170 180 190 200
QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF NRELIDFHLL GVALLAVAVF
210 220 230 240 250
ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY GTNEHLENIF
260 270 280 290 300
DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ
310 320 330
DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46259 Genomic DNA. Translation: CAA86372.1. Z71602 Genomic DNA. Translation: CAA96258.1. BK006947 Genomic DNA. Translation: DAA10237.1. |
PIRi | S55863. |
RefSeqi | NP_014073.1. NM_001183164.1. |
Genome annotation databases
EnsemblFungii | YNL326C; YNL326C; YNL326C. |
GeneIDi | 855390. |
KEGGi | sce:YNL326C. |
Similar proteinsi
Entry informationi
Entry namei | PFA3_YEAST | |
Accessioni | P42836Primary (citable) accession number: P42836 Secondary accession number(s): D6W0M1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | March 28, 2018 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |