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P42836

- PFA3_YEAST

UniProt

P42836 - PFA3_YEAST

Protein

Palmitoyltransferase PFA3

Gene

PFA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.1 Publication

    Catalytic activityi

    Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri104 – 15451DHHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. palmitoyltransferase activity Source: SGD
    2. protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein palmitoylation Source: SGD
    2. vacuole fusion, non-autophagic Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33310-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Palmitoyltransferase PFA3 (EC:2.3.1.225)
    Alternative name(s):
    Protein fatty acyltransferase 3
    Gene namesi
    Name:PFA3
    Ordered Locus Names:YNL326C
    ORF Names:N0325
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL326c.
    SGDiS000005270. PFA3.

    Subcellular locationi

    Vacuole membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. vacuolar membrane Source: UniProt

    Keywords - Cellular componenti

    Membrane, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi134 – 1341C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Palmitoyltransferase PFA3PRO_0000212959Add
    BLAST

    Post-translational modificationi

    Autopalmitoylated.

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP42836.

    Expressioni

    Gene expression databases

    GenevestigatoriP42836.

    Interactioni

    Protein-protein interaction databases

    BioGridi35515. 8 interactions.
    DIPiDIP-4833N.
    IntActiP42836. 2 interactions.
    MINTiMINT-479882.
    STRINGi4932.YNL326C.

    Structurei

    3D structure databases

    ProteinModelPortaliP42836.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini30 – 378VacuolarSequence Analysis
    Topological domaini59 – 14789CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini169 – 18820VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini210 – 336127CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei38 – 5821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The DHHC domain is required for palmitoyltransferase activity.

    Sequence similaritiesi

    Contains 1 DHHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri104 – 15451DHHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5273.
    GeneTreeiENSGT00750000117306.
    HOGENOMiHOG000246777.
    OMAiHYCPWFS.
    OrthoDBiEOG71P2MN.

    Family and domain databases

    InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
    [Graphical view]
    PfamiPF01529. zf-DHHC. 1 hit.
    [Graphical view]
    PROSITEiPS50216. ZF_DHHC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42836-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA    50
    LYTYYKVIAR GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD 100
    GRFRVCQVCH VWKPDRCHHC SSCDVCILKM DHHCPWFAEC TGFRNQKFFI 150
    QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF NRELIDFHLL GVALLAVAVF 200
    ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY GTNEHLENIF 250
    DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ 300
    DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN 336
    Length:336
    Mass (Da):39,184
    Last modified:November 1, 1995 - v1
    Checksum:iD36BF37DEC5D3983
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86372.1.
    Z71602 Genomic DNA. Translation: CAA96258.1.
    BK006947 Genomic DNA. Translation: DAA10237.1.
    PIRiS55863.
    RefSeqiNP_014073.1. NM_001183164.1.

    Genome annotation databases

    EnsemblFungiiYNL326C; YNL326C; YNL326C.
    GeneIDi855390.
    KEGGisce:YNL326C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46259 Genomic DNA. Translation: CAA86372.1 .
    Z71602 Genomic DNA. Translation: CAA96258.1 .
    BK006947 Genomic DNA. Translation: DAA10237.1 .
    PIRi S55863.
    RefSeqi NP_014073.1. NM_001183164.1.

    3D structure databases

    ProteinModelPortali P42836.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35515. 8 interactions.
    DIPi DIP-4833N.
    IntActi P42836. 2 interactions.
    MINTi MINT-479882.
    STRINGi 4932.YNL326C.

    Proteomic databases

    PaxDbi P42836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL326C ; YNL326C ; YNL326C .
    GeneIDi 855390.
    KEGGi sce:YNL326C.

    Organism-specific databases

    CYGDi YNL326c.
    SGDi S000005270. PFA3.

    Phylogenomic databases

    eggNOGi COG5273.
    GeneTreei ENSGT00750000117306.
    HOGENOMi HOG000246777.
    OMAi HYCPWFS.
    OrthoDBi EOG71P2MN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33310-MONOMER.

    Miscellaneous databases

    NextBioi 979199.

    Gene expression databases

    Genevestigatori P42836.

    Family and domain databases

    InterProi IPR001594. Znf_DHHC_palmitoyltrfase.
    [Graphical view ]
    Pfami PF01529. zf-DHHC. 1 hit.
    [Graphical view ]
    PROSITEi PS50216. ZF_DHHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
      Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
      J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiPFA3_YEAST
    AccessioniPrimary (citable) accession number: P42836
    Secondary accession number(s): D6W0M1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2133 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3