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Protein

Palmitoyltransferase PFA3

Gene

PFA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33310-MONOMER.
ReactomeiR-SCE-203615. eNOS activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase PFA3 (EC:2.3.1.225)
Alternative name(s):
Protein fatty acyltransferase 3
Gene namesi
Name:PFA3
Ordered Locus Names:YNL326C
ORF Names:N0325
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL326C.
SGDiS000005270. PFA3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 29HelicalSequence analysisAdd BLAST23
Topological domaini30 – 37VacuolarSequence analysis8
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 147CytoplasmicSequence analysisAdd BLAST89
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 188VacuolarSequence analysisAdd BLAST20
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 336CytoplasmicSequence analysisAdd BLAST127

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129591 – 336Palmitoyltransferase PFA3Add BLAST336

Post-translational modificationi

Autopalmitoylated.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PRIDEiP42836.

PTM databases

iPTMnetiP42836.

Interactioni

Protein-protein interaction databases

BioGridi35515. 8 interactors.
DIPiDIP-4833N.
IntActiP42836. 2 interactors.
MINTiMINT-479882.

Structurei

3D structure databases

ProteinModelPortaliP42836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 154DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Contains 1 DHHC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00850000132259.
HOGENOMiHOG000246777.
InParanoidiP42836.
KOiK20028.
OMAiHYCPWFS.
OrthoDBiEOG092C524D.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA
60 70 80 90 100
LYTYYKVIAR GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD
110 120 130 140 150
GRFRVCQVCH VWKPDRCHHC SSCDVCILKM DHHCPWFAEC TGFRNQKFFI
160 170 180 190 200
QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF NRELIDFHLL GVALLAVAVF
210 220 230 240 250
ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY GTNEHLENIF
260 270 280 290 300
DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ
310 320 330
DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN
Length:336
Mass (Da):39,184
Last modified:November 1, 1995 - v1
Checksum:iD36BF37DEC5D3983
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA. Translation: CAA86372.1.
Z71602 Genomic DNA. Translation: CAA96258.1.
BK006947 Genomic DNA. Translation: DAA10237.1.
PIRiS55863.
RefSeqiNP_014073.1. NM_001183164.1.

Genome annotation databases

EnsemblFungiiYNL326C; YNL326C; YNL326C.
GeneIDi855390.
KEGGisce:YNL326C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA. Translation: CAA86372.1.
Z71602 Genomic DNA. Translation: CAA96258.1.
BK006947 Genomic DNA. Translation: DAA10237.1.
PIRiS55863.
RefSeqiNP_014073.1. NM_001183164.1.

3D structure databases

ProteinModelPortaliP42836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35515. 8 interactors.
DIPiDIP-4833N.
IntActiP42836. 2 interactors.
MINTiMINT-479882.

PTM databases

iPTMnetiP42836.

Proteomic databases

PRIDEiP42836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL326C; YNL326C; YNL326C.
GeneIDi855390.
KEGGisce:YNL326C.

Organism-specific databases

EuPathDBiFungiDB:YNL326C.
SGDiS000005270. PFA3.

Phylogenomic databases

GeneTreeiENSGT00850000132259.
HOGENOMiHOG000246777.
InParanoidiP42836.
KOiK20028.
OMAiHYCPWFS.
OrthoDBiEOG092C524D.

Enzyme and pathway databases

BioCyciYEAST:G3O-33310-MONOMER.
ReactomeiR-SCE-203615. eNOS activation.

Miscellaneous databases

PROiP42836.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFA3_YEAST
AccessioniPrimary (citable) accession number: P42836
Secondary accession number(s): D6W0M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2133 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.