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P42836

- PFA3_YEAST

UniProt

P42836 - PFA3_YEAST

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Protein

Palmitoyltransferase PFA3

Gene

PFA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 15451DHHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. palmitoyltransferase activity Source: SGD
  2. protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. protein palmitoylation Source: SGD
  2. vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase PFA3 (EC:2.3.1.225)
Alternative name(s):
Protein fatty acyltransferase 3
Gene namesi
Name:PFA3
Ordered Locus Names:YNL326C
ORF Names:N0325
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL326c.
SGDiS000005270. PFA3.

Subcellular locationi

Vacuole membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. vacuolar membrane Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi134 – 1341C → S: Abolishes autopalmitoylation and VAC8 palmitoylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Palmitoyltransferase PFA3PRO_0000212959Add
BLAST

Post-translational modificationi

Autopalmitoylated.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP42836.

Expressioni

Gene expression databases

GenevestigatoriP42836.

Interactioni

Protein-protein interaction databases

BioGridi35515. 9 interactions.
DIPiDIP-4833N.
IntActiP42836. 2 interactions.
MINTiMINT-479882.
STRINGi4932.YNL326C.

Structurei

3D structure databases

ProteinModelPortaliP42836.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini30 – 378VacuolarSequence Analysis
Topological domaini59 – 14789CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini169 – 18820VacuolarSequence AnalysisAdd
BLAST
Topological domaini210 – 336127CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923HelicalSequence AnalysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Transmembranei189 – 20921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Contains 1 DHHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 15451DHHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiCOG5273.
GeneTreeiENSGT00760000119256.
HOGENOMiHOG000246777.
InParanoidiP42836.
OMAiHYCPWFS.
OrthoDBiEOG71P2MN.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. ZF_DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42836-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNDRLSLTSL FPRCLTTCLY IWTAYITLTR IHQIPRWFLA LTIVPTLAVA
60 70 80 90 100
LYTYYKVIAR GPGSPLDFPD LLVHDLKAAE NGLELPPEYM SKRCLTLKHD
110 120 130 140 150
GRFRVCQVCH VWKPDRCHHC SSCDVCILKM DHHCPWFAEC TGFRNQKFFI
160 170 180 190 200
QFLMYTTLYA FLVLIYTCYE LGTWFNSGSF NRELIDFHLL GVALLAVAVF
210 220 230 240 250
ISVLAFTCFS IYQVCKNQTT IEVHGMRRYR RDLEILNDSY GTNEHLENIF
260 270 280 290 300
DLGSSMANWQ DIMGTSWLEW ILPIETFKYK KSKHTKDEKG LYFNVRPQVQ
310 320 330
DRLLSSRCLE DQLLRRVTPR PSLEADRASV EIIDAN
Length:336
Mass (Da):39,184
Last modified:November 1, 1995 - v1
Checksum:iD36BF37DEC5D3983
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86372.1.
Z71602 Genomic DNA. Translation: CAA96258.1.
BK006947 Genomic DNA. Translation: DAA10237.1.
PIRiS55863.
RefSeqiNP_014073.1. NM_001183164.1.

Genome annotation databases

EnsemblFungiiYNL326C; YNL326C; YNL326C.
GeneIDi855390.
KEGGisce:YNL326C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46259 Genomic DNA. Translation: CAA86372.1 .
Z71602 Genomic DNA. Translation: CAA96258.1 .
BK006947 Genomic DNA. Translation: DAA10237.1 .
PIRi S55863.
RefSeqi NP_014073.1. NM_001183164.1.

3D structure databases

ProteinModelPortali P42836.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35515. 9 interactions.
DIPi DIP-4833N.
IntActi P42836. 2 interactions.
MINTi MINT-479882.
STRINGi 4932.YNL326C.

Proteomic databases

PaxDbi P42836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL326C ; YNL326C ; YNL326C .
GeneIDi 855390.
KEGGi sce:YNL326C.

Organism-specific databases

CYGDi YNL326c.
SGDi S000005270. PFA3.

Phylogenomic databases

eggNOGi COG5273.
GeneTreei ENSGT00760000119256.
HOGENOMi HOG000246777.
InParanoidi P42836.
OMAi HYCPWFS.
OrthoDBi EOG71P2MN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33310-MONOMER.

Miscellaneous databases

NextBioi 979199.

Gene expression databases

Genevestigatori P42836.

Family and domain databases

InterProi IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view ]
Pfami PF01529. zf-DHHC. 1 hit.
[Graphical view ]
PROSITEi PS50216. ZF_DHHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
    Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
    J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-134, SUBCELLULAR LOCATION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiPFA3_YEAST
AccessioniPrimary (citable) accession number: P42836
Secondary accession number(s): D6W0M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2133 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3