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P42835 (EGT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein EGT2
Alternative name(s):
Early G1 transcript 2
Gene names
Name:EGT2
Ordered Locus Names:YNL327W
ORF Names:N0320
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length1041 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Seems to be involved in the correct timing of cell separation after cytokinesis, as separation of mutant daughter cells is delayed. Could either be an enzyme necessary for glucans-degradation of the cell wall at the neck region between mother and daughter cells or a regulatory protein controlling this metabolic step. Ref.1

Subcellular location

Secreted › cell wall Probable. Membrane; Lipid-anchor › GPI-anchor Potential. Note: Localizes to the septum of dividing cells. Ref.5 Ref.6

Induction

Exclusively expressed between the end of mitosis and early G1; inactivated before cells pass start. Ref.1

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 10201000Protein EGT2
PRO_0000021159
Propeptide1021 – 104121Removed in mature form Potential
PRO_0000372451

Regions

Repeat457 – 492361
Repeat577 – 606302
Repeat613 – 647353
Repeat716 – 745304
Repeat773 – 802305
Repeat811 – 840306
Repeat849 – 886387
Repeat887 – 924388
Repeat925 – 962389
Region457 – 9625069 X approximate repeats
Compositional bias301 – 764464Ser-rich
Compositional bias403 – 1003601Thr-rich

Amino acid modifications

Lipidation10201GPI-anchor amidated glycine Potential
Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential
Glycosylation4011N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation4851N-linked (GlcNAc...) Potential
Glycosylation5061N-linked (GlcNAc...) Potential
Glycosylation5261N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential
Glycosylation5561N-linked (GlcNAc...) Potential
Glycosylation6351N-linked (GlcNAc...) Potential
Glycosylation6361N-linked (GlcNAc...) Potential
Glycosylation6571N-linked (GlcNAc...) Potential
Glycosylation7091N-linked (GlcNAc...) Potential
Glycosylation7561N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5771T → A Ref.1
Sequence conflict5771T → A in CAA96259. Ref.2
Sequence conflict5771T → A in CAA86371. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P42835 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 64C987E1376581FA

FASTA1,041108,525
        10         20         30         40         50         60 
MNKLLLHLVR VISILGLANA LTQTQPILKD IQITDSYTKT KECTDPDHWF IIEGQLSIPK 

        70         80         90        100        110        120 
GSQQNITFQV PDAFSSFPQE PFSIKHNSNS VATISRPDKS TNNFTISIPE KSSEDITTTF 

       130        140        150        160        170        180 
NFLAQLTSDA KSKVTEPKSI VYSFYSENTM FNDVIDYVAK NTSAITTDGG IYKTNNTAWF 

       190        200        210        220        230        240 
TVDLPMRTFR NPVYLTSQTS SSSDYVFDTS LTKFEVVTAV DSFNEPINAI PYTTVHDYST 

       250        260        270        280        290        300 
EDEIRCLFNS TISGGLYFRV TYFTKKLSTS SISNTVELTY PDEGTSVRLL GKRDTSTTLA 

       310        320        330        340        350        360 
SELYSESAAN IDSTTSDDTT SSDAAITPTY SNSTLSSYTS QSSAIPEVAV TASLSSGILS 

       370        380        390        400        410        420 
STVDGASTSA DASMSAVSTV SSSSEQASSS SISLSAPSSS NSTFTTPSSS LSATETYSII 

       430        440        450        460        470        480 
SSASISVTQA SYIDNSTTTA VTQSTSTIAV SSAEKLSSTL SYTSNVTISV SSATQHTTTP 

       490        500        510        520        530        540 
SYVSNSTTLS SSSVLESVIS SPYLANTTVS GASSASQSTN PPYVSNSTTS SATQLATIAP 

       550        560        570        580        590        600 
FAINITGTSI SSSITNTSSV SSTTSSLSSG PFVSNTTVAS GSYILTTTTE SAQLTEIGSL 

       610        620        630        640        650        660 
IPISTITTST TTSGTDKTGS NKVASSTEIA QSIVNNSSLS VSTINTNAAT AAANARNATF 

       670        680        690        700        710        720 
THATHSGSLQ PSYHSSSLLS STIDTKVTTA TTSTSRDGSS SLAFTTGLNQ SVVTGTDKSD 

       730        740        750        760        770        780 
TYSVISSTES AQVTEYDSLL PISTLKPTVV TGTSRNSTFS MVSSTKLTEA TATDKGDAYS 

       790        800        810        820        830        840 
VISSTQSAQV TEYGSMLPIS TLETPTVIMS TDESGYFTLT TCTESGQATE YGSLIPISTL 

       850        860        870        880        890        900 
DGSVIYTFTG ESVVVGYSTT VGAAQYAQHT SLVPVSTIKG SKTSLSTEES VVAGYSTTVG 

       910        920        930        940        950        960 
AAQYAQHTSL VPVSTIKGSK TSLSTEESVV AGYSTTVDSA QYAEHTNLVA IDTLKTSTFQ 

       970        980        990       1000       1010       1020 
KATATEVCVT CTALSSPHSA TLDAGTTISL PTSSSTSLST IITWYSSSTI KPPSISTYSG 

      1030       1040 
AAGQLTIRIG SLLLGLISFL L

« Hide

References

« Hide 'large scale' references
[1]"EGT2 gene transcription is induced predominantly by Swi5 in early G1."
Kovacech B., Nasmyth K., Schuster T.
Mol. Cell. Biol. 16:3264-3274(1996) [PubMed: 8668141] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION.
[2]"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed: 9169873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 577.
Strain: ATCC 204508 / S288c.
[5]"Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
Mol. Gen. Genet. 258:53-59(1998) [PubMed: 9613572] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"GPI7 involved in glycosylphosphatidylinositol biosynthesis is essential for yeast cell separation."
Fujita M., Yoko-o T., Okamoto M., Jigami Y.
J. Biol. Chem. 279:51869-51879(2004) [PubMed: 15452134] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract]
Cited for: REPEATS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z46259 Genomic DNA. Translation: CAA86371.1.
Z71603 Genomic DNA. Translation: CAA96259.1.
BK006947 Genomic DNA. Translation: DAA10236.2.
PIRS55862.
RefSeqNP_014072.2. NM_001183165.2.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4267N.
MINTMINT-545218.
STRINGP42835.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID855389.
KEGGsce:YNL327W.
NMPDRfig|4932.3.peg.5134.

Organism-specific databases

CYGDYNL327w.
SGDS000005271. EGT2.

Phylogenomic databases

eggNOGfuNOG11792.
GeneTreeEFGT00050000000104.
OrthoDBEOG4KM2CP.

Gene expression databases

ArrayExpressP42835.
GenevestigatorP42835.
GermOnlineYNL327W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other

NextBio979196.

Entry information

Entry nameEGT2_YEAST
AccessionPrimary (citable) accession number: P42835
Secondary accession number(s): D6W0M0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 21, 2011
Last modified: January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents