ID   MDJ2_YEAST              Reviewed;         146 AA.
AC   P42834; D6W0L9;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Mitochondrial DnaJ homolog 2;
GN   Name=MDJ2; OrderedLocusNames=YNL328C; ORFNames=N0315;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / FY1676;
RX   PubMed=7645347; DOI=10.1002/yea.320110606;
RA   Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.;
RT   "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV
RT   identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames.";
RL   Yeast 11:567-572(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9325105; DOI=10.1006/jmbi.1997.1267;
RA   Westermann B., Neupert W.;
RT   "Mdj2p, a novel DnaJ homolog in the mitochondrial inner membrane of the
RT   yeast Saccharomyces cerevisiae.";
RL   J. Mol. Biol. 272:477-483(1997).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   FUNCTION, IDENTIFICATION IN THE PAM23 COMPLEX, AND INTERACTION WITH PAM16.
RX   PubMed=16027163; DOI=10.1074/jbc.m502397200;
RA   Mokranjac D., Sichting M., Popov-Celeketic D., Berg A., Hell K.,
RA   Neupert W.;
RT   "The import motor of the yeast mitochondrial TIM23 preprotein translocase
RT   contains two different J proteins, Tim14 and Mdj2.";
RL   J. Biol. Chem. 280:31608-31614(2005).
CC   -!- FUNCTION: Plays a role in mitochondrial biogenesis and protein folding.
CC       Participates in the translocation of transit peptide-containing
CC       proteins from the inner membrane into the mitochondrial matrix in an
CC       ATP-dependent manner, probably by stimulating activity of mtHSP70
CC       (SSC1). {ECO:0000269|PubMed:16027163, ECO:0000269|PubMed:9325105}.
CC   -!- SUBUNIT: Interacts with PAM16/TIM16 and is recruited by the PAM
CC       complex. {ECO:0000269|PubMed:16027163}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:9325105}.
CC   -!- MISCELLANEOUS: Present with 573 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z46259; CAA86370.1; -; Genomic_DNA.
DR   EMBL; Z71604; CAA96260.1; -; Genomic_DNA.
DR   EMBL; AY692768; AAT92787.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10235.1; -; Genomic_DNA.
DR   PIR; S55861; S55861.
DR   RefSeq; NP_014071.1; NM_001183166.1.
DR   AlphaFoldDB; P42834; -.
DR   SMR; P42834; -.
DR   BioGRID; 35513; 28.
DR   DIP; DIP-4690N; -.
DR   STRING; 4932.YNL328C; -.
DR   MoonDB; P42834; Predicted.
DR   TCDB; 3.A.8.1.1; the mitochondrial protein translocase (mpt) family.
DR   MaxQB; P42834; -.
DR   PaxDb; 4932-YNL328C; -.
DR   PeptideAtlas; P42834; -.
DR   EnsemblFungi; YNL328C_mRNA; YNL328C; YNL328C.
DR   GeneID; 855388; -.
DR   KEGG; sce:YNL328C; -.
DR   AGR; SGD:S000005272; -.
DR   SGD; S000005272; MDJ2.
DR   VEuPathDB; FungiDB:YNL328C; -.
DR   eggNOG; KOG0723; Eukaryota.
DR   HOGENOM; CLU_017633_13_4_1; -.
DR   InParanoid; P42834; -.
DR   OMA; WQAFKLR; -.
DR   OrthoDB; 160310at2759; -.
DR   BioCyc; YEAST:G3O-33312-MONOMER; -.
DR   BioGRID-ORCS; 855388; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P42834; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P42834; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IPI:SGD.
DR   GO; GO:0001671; F:ATPase activator activity; IDA:SGD.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IGI:SGD.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; DnaJ domain; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   PANTHER; PTHR12763:SF29; MITOCHONDRIAL DNAJ HOMOLOG 2; 1.
DR   PANTHER; PTHR12763; UNCHARACTERIZED; 1.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; Chaperone J-domain; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Stress response; Translocation;
KW   Transport.
FT   CHAIN           1..146
FT                   /note="Mitochondrial DnaJ homolog 2"
FT                   /id="PRO_0000071094"
FT   DOMAIN          85..146
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ   SEQUENCE   146 AA;  16474 MW;  36AC6362E15CEE71 CRC64;
     MVLPIIIGLG VTMVALSVKS GLNAWTVYKT LSPLTIAKLN NIRIENPTAG YRDALKFKSS
     LIDEELKNRL NQYQGGFAPR MTEPEALLIL DISAREINHL DEKLLKKKHR KAMVRNHPDR
     GGSPYMAAKI NEAKEVLERS VLLRKR
//