Reviewed, UniProtKB/Swiss-Prot P42834 (MDJ2_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial DnaJ homolog 2

Gene names

Name:	MDJ2
Ordered Locus Names:	YNL328C
ORF Names:	N0315

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in mitochondrial biogenesis and protein folding. Participates in the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner, probably by stimulating activity of mtHSP70 (SSC1). Ref.4 Ref.7
Subunit structure	Interacts with PAM16/TIM16 and is recruited by the PAM complex. Ref.7
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 573 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Biological process	Protein transport Stress response Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial matrix Ref.7 Inferred from genetic interaction. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	presequence translocase-associated import motor Ref.7 Inferred from physical interaction. Source: SGD
Molecular function	ATPase activator activity Ref.7 Inferred from direct assay. Source: SGD heat shock protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 146	146	Mitochondrial DnaJ homolog 2		PRO_0000071094
Regions
	Domain	85 – 146	62	J

Sequences

	Sequence		Length	Mass (Da)
	P42834-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 36AC6362E15CEE71	FASTA	146	16,474
	10 20 30 40 50 60 MVLPIIIGLG VTMVALSVKS GLNAWTVYKT LSPLTIAKLN NIRIENPTAG YRDALKFKSS 70 80 90 100 110 120 LIDEELKNRL NQYQGGFAPR MTEPEALLIL DISAREINHL DEKLLKKKHR KAMVRNHPDR 130 140 GGSPYMAAKI NEAKEVLERS VLLRKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Mdj2p, a novel DnaJ homolog in the mitochondrial inner membrane of the yeast Saccharomyces cerevisiae." Westermann B., Neupert W. J. Mol. Biol. 272:477-483(1997) [PubMed: 9325105] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2." Mokranjac D., Sichting M., Popov-Celeketic D., Berg A., Hell K., Neupert W. J. Biol. Chem. 280:31608-31614(2005) [PubMed: 16027163] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE PAM23 COMPLEX, INTERACTION WITH PAM16.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86370.1. Z71604 Genomic DNA. Translation: CAA96260.1. AY692768 Genomic DNA. Translation: AAT92787.1.
PIR	S55861.
RefSeq	NP_014071.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4690N.
IntAct	P42834. 1 interaction.
STRING	P42834.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Genome annotation databases
Ensembl	YNL328C; YNL328C; YNL328C; Saccharomyces cerevisiae. [Genome view]
GeneID	855388.
KEGG	sce:YNL328C.
NMPDR	fig|4932.3.peg.5133.
Organism-specific databases
CYGD	YNL328c.
SGD	S000005272. MDJ2.
Phylogenomic databases
HOGENOM	P42834.
OMA	DRYRKLM
OrthoDB	EOG9T4FBV
Gene expression databases
ArrayExpress	P42834.
Genevestigator	P42834.
GermOnline	YNL328C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001623. DnaJ_N. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979193.

Entry information

Entry name

MDJ2_YEAST

Accession

Primary (citable) accession number: P42834

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)