P42834 (MDJ2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial DnaJ homolog 2

Gene names

Name:	MDJ2
Ordered Locus Names:	YNL328C
ORF Names:	N0315

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in mitochondrial biogenesis and protein folding. Participates in the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner, probably by stimulating activity of mtHSP70 (SSC1). Ref.5 Ref.8
Subunit structure	Interacts with PAM16/TIM16 and is recruited by the PAM complex. Ref.8
Subcellular location	Mitochondrion inner membrane Ref.5 Ref.6.
Miscellaneous	Present with 573 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Biological process	Protein transport Stress response Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial matrix Inferred from genetic interaction Ref.8. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	presequence translocase-associated import motor Inferred from physical interaction Ref.8. Source: SGD
Molecular_function	ATPase activator activity Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 146	146	Mitochondrial DnaJ homolog 2		PRO_0000071094
Regions
	Domain	85 – 146	62	J

Sequences

	Sequence		Length	Mass (Da)
	P42834 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 36AC6362E15CEE71	FASTA	146	16,474
	10 20 30 40 50 60 MVLPIIIGLG VTMVALSVKS GLNAWTVYKT LSPLTIAKLN NIRIENPTAG YRDALKFKSS 70 80 90 100 110 120 LIDEELKNRL NQYQGGFAPR MTEPEALLIL DISAREINHL DEKLLKKKHR KAMVRNHPDR 130 140 GGSPYMAAKI NEAKEVLERS VLLRKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Mdj2p, a novel DnaJ homolog in the mitochondrial inner membrane of the yeast Saccharomyces cerevisiae." Westermann B., Neupert W. J. Mol. Biol. 272:477-483(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2." Mokranjac D., Sichting M., Popov-Celeketic D., Berg A., Hell K., Neupert W. J. Biol. Chem. 280:31608-31614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE PAM23 COMPLEX, INTERACTION WITH PAM16.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46259 Genomic DNA. Translation: CAA86370.1. Z71604 Genomic DNA. Translation: CAA96260.1. AY692768 Genomic DNA. Translation: AAT92787.1. BK006947 Genomic DNA. Translation: DAA10235.1.
PIR	S55861.
RefSeq	NP_014071.1. NM_001183166.1.
3D structure databases
ProteinModelPortal	P42834.
SMR	P42834. Positions 74-139.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4690N.
MINT	MINT-556272.
STRING	4932.YNL328C.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PaxDb	P42834.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL328C; YNL328C; YNL328C.
GeneID	855388.
KEGG	sce:YNL328C.
Organism-specific databases
CYGD	YNL328c.
SGD	S000005272. MDJ2.
Phylogenomic databases
eggNOG	COG2214.
HOGENOM	HOG000115841.
OMA	DRYRKLM.
OrthoDB	EOG4QJVXX.
Gene expression databases
ArrayExpress	P42834.
Genevestigator	P42834.
GermOnline	YNL328C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR001623. DnaJ_domain. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979193.

Entry information

Entry name

MDJ2_YEAST

Accession

Primary (citable) accession number: P42834
Secondary accession number(s): D6W0L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program