ID   CCL2_PIG                Reviewed;          99 AA.
AC   P42831;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   24-JAN-2024, entry version 141.
DE   RecName: Full=C-C motif chemokine 2;
DE   AltName: Full=Monocyte chemoattractant protein 1;
DE   AltName: Full=Monocyte chemotactic protein 1;
DE            Short=MCP-1;
DE   AltName: Full=Small-inducible cytokine A2;
DE   Flags: Precursor;
GN   Name=CCL2; Synonyms=SCYA2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7510962; DOI=10.1006/bbrc.1994.1323;
RA   Hosang K., Knoke I., Klaudiny J., Wempe F., Wuttke W., Scheit K.H.;
RT   "Porcine luteal cells express monocyte chemoattractant protein-1 (MCP-1):
RT   analysis by polymerase chain reaction and cDNA cloning.";
RL   Biochem. Biophys. Res. Commun. 199:962-968(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RA   Zach O.R.F.;
RL   Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a ligand for C-C chemokine receptor CCR2 (By
CC       similarity). Signals through binding and activation of CCR2 and induces
CC       a strong chemotactic response and mobilization of intracellular calcium
CC       ions (By similarity). Exhibits a chemotactic activity for monocytes and
CC       basophils but not neutrophils or eosinophils (By similarity). Plays an
CC       important role in mediating peripheral nerve injury-induced neuropathic
CC       pain (By similarity). Increases NMDA-mediated synaptic transmission in
CC       both dopamine D1 and D2 receptor-containing neurons, which may be
CC       caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By
CC       similarity). {ECO:0000250|UniProtKB:P10148,
CC       ECO:0000250|UniProtKB:P13500}.
CC   -!- SUBUNIT: Monomer or homodimer; in equilibrium. Is tethered on
CC       endothelial cells by glycosaminoglycan (GAG) side chains of
CC       proteoglycans. Interacts with TNFAIP6 (via Link domain).
CC       {ECO:0000250|UniProtKB:P13500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P13500}.
CC   -!- PTM: Processing at the N-terminus can regulate receptor and target cell
CC       selectivity (By similarity). Deletion of the N-terminal residue
CC       converts it from an activator of basophil to an eosinophil
CC       chemoattractant (By similarity). {ECO:0000250|UniProtKB:P13500}.
CC   -!- PTM: N-Glycosylated. {ECO:0000250|UniProtKB:P13500}.
CC   -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z48479; CAA88370.1; -; mRNA.
DR   EMBL; X79416; CAA55945.1; -; mRNA.
DR   PIR; JC2136; JC2136.
DR   RefSeq; NP_999379.1; NM_214214.1.
DR   AlphaFoldDB; P42831; -.
DR   SMR; P42831; -.
DR   STRING; 9823.ENSSSCP00000018779; -.
DR   PaxDb; 9823-ENSSSCP00000018779; -.
DR   Ensembl; ENSSSCT00000019290.5; ENSSSCP00000018779.2; ENSSSCG00000017723.5.
DR   Ensembl; ENSSSCT00005020545.1; ENSSSCP00005012371.1; ENSSSCG00005013096.1.
DR   Ensembl; ENSSSCT00015014476.1; ENSSSCP00015005616.1; ENSSSCG00015010935.1.
DR   Ensembl; ENSSSCT00030075613.1; ENSSSCP00030034551.1; ENSSSCG00030054240.1.
DR   Ensembl; ENSSSCT00035084936.1; ENSSSCP00035035342.1; ENSSSCG00035063168.1.
DR   Ensembl; ENSSSCT00040012972.1; ENSSSCP00040004899.1; ENSSSCG00040010004.1.
DR   Ensembl; ENSSSCT00045031268.1; ENSSSCP00045021669.1; ENSSSCG00045018363.1.
DR   Ensembl; ENSSSCT00050053082.1; ENSSSCP00050022292.1; ENSSSCG00050039348.1.
DR   Ensembl; ENSSSCT00055016609.1; ENSSSCP00055013103.1; ENSSSCG00055008496.1.
DR   Ensembl; ENSSSCT00060077021.1; ENSSSCP00060033287.1; ENSSSCG00060056544.1.
DR   Ensembl; ENSSSCT00065092116.1; ENSSSCP00065040308.1; ENSSSCG00065067117.1.
DR   Ensembl; ENSSSCT00070015610.1; ENSSSCP00070012916.1; ENSSSCG00070008075.1.
DR   GeneID; 397422; -.
DR   KEGG; ssc:397422; -.
DR   CTD; 6347; -.
DR   eggNOG; ENOG502S6ZP; Eukaryota.
DR   GeneTree; ENSGT01100000263482; -.
DR   HOGENOM; CLU_141716_1_0_1; -.
DR   InParanoid; P42831; -.
DR   OMA; PITCCYT; -.
DR   OrthoDB; 4265193at2759; -.
DR   TreeFam; TF334888; -.
DR   Reactome; R-SSC-380108; Chemokine receptors bind chemokines.
DR   Proteomes; UP000008227; Chromosome 12.
DR   Proteomes; UP000314985; Chromosome 12.
DR   Proteomes; UP000694570; Unplaced.
DR   Proteomes; UP000694571; Unplaced.
DR   Proteomes; UP000694720; Unplaced.
DR   Proteomes; UP000694722; Unplaced.
DR   Proteomes; UP000694723; Unplaced.
DR   Proteomes; UP000694724; Unplaced.
DR   Proteomes; UP000694725; Unplaced.
DR   Proteomes; UP000694726; Unplaced.
DR   Proteomes; UP000694727; Unplaced.
DR   Proteomes; UP000694728; Unplaced.
DR   Bgee; ENSSSCG00000017723; Expressed in endocardial endothelium and 42 other cell types or tissues.
DR   ExpressionAtlas; P42831; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR   GO; GO:0043615; P:astrocyte cell migration; IEA:Ensembl.
DR   GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEA:Ensembl.
DR   GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR   GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR   GO; GO:0071346; P:cellular response to type II interferon; IBA:GO_Central.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR   GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:0048246; P:macrophage chemotaxis; IEA:Ensembl.
DR   GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IEA:Ensembl.
DR   GO; GO:0034351; P:negative regulation of glial cell apoptotic process; IEA:Ensembl.
DR   GO; GO:2000502; P:negative regulation of natural killer cell chemotaxis; IEA:Ensembl.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Ensembl.
DR   GO; GO:1905563; P:negative regulation of vascular endothelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR   GO; GO:0090280; P:positive regulation of calcium ion import; IEA:Ensembl.
DR   GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; IEA:Ensembl.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR   GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISS:UniProtKB.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:Ensembl.
DR   GO; GO:0019233; P:sensory perception of pain; ISS:UniProtKB.
DR   CDD; cd00272; Chemokine_CC; 1.
DR   Gene3D; 2.40.50.40; -; 1.
DR   InterPro; IPR039809; Chemokine_b/g/d.
DR   InterPro; IPR000827; Chemokine_CC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR12015:SF98; C-C MOTIF CHEMOKINE 2; 1.
DR   PANTHER; PTHR12015; SMALL INDUCIBLE CYTOKINE A; 1.
DR   Pfam; PF00048; IL8; 1.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; Interleukin 8-like chemokines; 1.
DR   PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
DR   Genevisible; P42831; SS.
PE   3: Inferred from homology;
KW   Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..99
FT                   /note="C-C motif chemokine 2"
FT                   /id="PRO_0000005153"
FT   MOD_RES         24
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P13500"
FT   DISULFID        34..59
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   99 AA;  10976 MW;  4C0AC6278D4F0A09 CRC64;
     MKVSAALLCL LLTAATFCTQ VLAQPDAINS PVTCCYTLTS KKISMQRLMS YRRVTSSKCP
     KEAVIFKTIA GKEICAEPKQ KWVQDSISHL DKKNQTPKP
//