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Protein

C-X-C motif chemokine 5

Gene

CXCL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei44 – 452Cleavage; by cathepsin G

GO - Molecular functioni

  1. chemokine activity Source: ProtInc
  2. CXCR chemokine receptor binding Source: GO_Central

GO - Biological processi

  1. cell-cell signaling Source: ProtInc
  2. cell chemotaxis Source: GOC
  3. chemokine-mediated signaling pathway Source: GO_Central
  4. chemotaxis Source: ProtInc
  5. G-protein coupled receptor signaling pathway Source: GO_Central
  6. immune response Source: GO_Central
  7. inflammatory response Source: GO_Central
  8. neutrophil mediated immunity Source: GO_Central
  9. positive regulation of cell proliferation Source: ProtInc
  10. positive regulation of leukocyte chemotaxis Source: GO_Central
  11. response to lipopolysaccharide Source: GO_Central
  12. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

ReactomeiREACT_15344. Chemokine receptors bind chemokines.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-X-C motif chemokine 5
Alternative name(s):
ENA-78(1-78)
Epithelial-derived neutrophil-activating protein 78
Neutrophil-activating peptide ENA-78
Small-inducible cytokine B5
Cleaved into the following 2 chains:
Gene namesi
Name:CXCL5
Synonyms:ENA78, SCYB5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:10642. CXCL5.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35573.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 36362 PublicationsAdd
BLAST
Chaini37 – 11478C-X-C motif chemokine 5PRO_0000005075Add
BLAST
Chaini44 – 11471ENA-78(8-78)PRO_0000005076Add
BLAST
Chaini45 – 11470ENA-78(9-78)PRO_0000005077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 75By similarity
Disulfide bondi51 ↔ 91By similarity

Post-translational modificationi

N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP42830.
PaxDbiP42830.
PRIDEiP42830.

PTM databases

PhosphoSiteiP42830.

Miscellaneous databases

PMAP-CutDBP42830.

Expressioni

Gene expression databases

BgeeiP42830.
CleanExiHS_CXCL5.
ExpressionAtlasiP42830. baseline and differential.
GenevestigatoriP42830.

Organism-specific databases

HPAiHPA065474.

Interactioni

Protein-protein interaction databases

BioGridi112276. 2 interactions.
DIPiDIP-5911N.
STRINGi9606.ENSP00000296027.

Structurei

Secondary structure

1
114
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi60 – 623Combined sources
Beta strandi63 – 697Combined sources
Beta strandi79 – 879Combined sources
Beta strandi89 – 924Combined sources
Helixi97 – 10812Combined sources
Turni109 – 1124Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MGSNMR-A/B44-114[»]
ProteinModelPortaliP42830.
SMRiP42830. Positions 44-114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44673.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP42830.
KOiK05506.
OMAiLRCVCLQ.
OrthoDBiEOG7B5WZS.
PhylomeDBiP42830.
TreeFamiTF333433.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSSRAAR VPGPSSSLCA LLVLLLLLTQ PGPIASAGPA AAVLRELRCV
60 70 80 90 100
CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK
110
KVIQKILDGG NKEN
Length:114
Mass (Da):11,972
Last modified:October 31, 1995 - v1
Checksum:i56B21EE86AE952D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78686 mRNA. Translation: CAA55355.1.
U12709 Genomic DNA. Translation: AAA62475.1.
L37036 Genomic DNA. Translation: AAA86426.1.
AF349466 Genomic DNA. Translation: AAK29641.1.
BC008376 mRNA. Translation: AAH08376.1.
AJ315732 Genomic DNA. Translation: CAC42884.1.
CCDSiCCDS34006.1.
PIRiJC2433. A55010.
RefSeqiNP_002985.1. NM_002994.4.
UniGeneiHs.89714.

Genome annotation databases

EnsembliENST00000296027; ENSP00000296027; ENSG00000163735.
GeneIDi6374.
KEGGihsa:6374.
UCSCiuc003hhk.4. human.

Polymorphism databases

DMDMi1169525.

Cross-referencesi

Web resourcesi

Wikipedia

CXCL5 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78686 mRNA. Translation: CAA55355.1.
U12709 Genomic DNA. Translation: AAA62475.1.
L37036 Genomic DNA. Translation: AAA86426.1.
AF349466 Genomic DNA. Translation: AAK29641.1.
BC008376 mRNA. Translation: AAH08376.1.
AJ315732 Genomic DNA. Translation: CAC42884.1.
CCDSiCCDS34006.1.
PIRiJC2433. A55010.
RefSeqiNP_002985.1. NM_002994.4.
UniGeneiHs.89714.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MGSNMR-A/B44-114[»]
ProteinModelPortaliP42830.
SMRiP42830. Positions 44-114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112276. 2 interactions.
DIPiDIP-5911N.
STRINGi9606.ENSP00000296027.

PTM databases

PhosphoSiteiP42830.

Polymorphism databases

DMDMi1169525.

Proteomic databases

MaxQBiP42830.
PaxDbiP42830.
PRIDEiP42830.

Protocols and materials databases

DNASUi6374.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296027; ENSP00000296027; ENSG00000163735.
GeneIDi6374.
KEGGihsa:6374.
UCSCiuc003hhk.4. human.

Organism-specific databases

CTDi6374.
GeneCardsiGC04M074851.
HGNCiHGNC:10642. CXCL5.
HPAiHPA065474.
MIMi600324. gene.
neXtProtiNX_P42830.
PharmGKBiPA35573.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG44673.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP42830.
KOiK05506.
OMAiLRCVCLQ.
OrthoDBiEOG7B5WZS.
PhylomeDBiP42830.
TreeFamiTF333433.

Enzyme and pathway databases

ReactomeiREACT_15344. Chemokine receptors bind chemokines.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiCXCL5.
GenomeRNAii6374.
NextBioi24764.
PMAP-CutDBP42830.
PROiP42830.
SOURCEiSearch...

Gene expression databases

BgeeiP42830.
CleanExiHS_CXCL5.
ExpressionAtlasiP42830. baseline and differential.
GenevestigatoriP42830.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a full-length cDNA encoding the neutrophil-activating peptide ENA-78 from human platelets."
    Power C.A., Furness R.B., Brawand C., Wells T.N.C.
    Gene 151:333-334(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of the human neutrophil-activating peptide (ENA-78) gene."
    Chang M.S., McNinch J., Basu R., Simonet S.
    J. Biol. Chem. 269:25277-25282(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Characterization of the gene for human neutrophil-activating peptide 78 (ENA-78)."
    Corbett M.S., Schmitt I., Riess O., Walz A.
    Biochem. Biophys. Res. Commun. 205:612-617(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus."
    Zhang C., Thornton M.A., Kowalska M.A., Sachis B.S., Feldman M., Poncz M., McKenzie S.E., Reilly M.P.
    Blood 98:610-617(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  6. "Novel polymorphism in ENA-78 gene."
    Amoli M.M., Thomson W., Hajeer A.H., Gonzalez-Gay M.A., Ollier W.E.R.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-80.
  7. "Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to interleukin 8."
    Walz A., Burgener R., Car B., Baggiolini M., Kunkel S.L., Strieter R.M.
    J. Exp. Med. 174:1355-1362(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-114.
    Tissue: Epithelium.
  8. "Isolation of the CXC chemokines ENA-78, GRO alpha and GRO gamma from tumor cells and leukocytes reveals NH2-terminal heterogeneity. Functional comparison of different natural isoforms."
    Wuyts A., Govaerts C., Struyf S., Lenaerts J.-P., Put W., Conings R., Proost P., Van Damme J.
    Eur. J. Biochem. 260:421-429(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-51, IDENTIFICATION OF ENA-78(8-78) AND ENA-78(9-78), PROTEOLYTIC PROCESSING OF N-TERMINUS, FUNCTION.
    Tissue: Peripheral blood monocyte.
  9. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-45.
    Tissue: Platelet.
  10. "Regulation of the immune response by the interaction of chemokines and proteases."
    Struyf S., Proost P., Van Damme J.
    Adv. Immunol. 81:1-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCXCL5_HUMAN
AccessioniPrimary (citable) accession number: P42830
Secondary accession number(s): Q96QE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: March 3, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.