P42830 (CXCL5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C motif chemokine 5

Alternative name(s):
ENA-78(1-78)
Epithelial-derived neutrophil-activating protein 78
Neutrophil-activating peptide ENA-78
Small-inducible cytokine B5

Cleaved into the following 2 chains:

1. ENA-78(8-78)
2. ENA-78(9-78)

Gene names

Name:	CXCL5
Synonyms:	ENA78, SCYB5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Ref.8
Subcellular location	Secreted.
Post-translational modification	N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.2. Source: ProtInc chemotaxis Traceable author statement PubMed 10820279. Source: ProtInc immune response Inferred from electronic annotation. Source: InterPro positive regulation of cell proliferation Traceable author statement PubMed 10463948. Source: ProtInc signal transduction Traceable author statement PubMed 10820279. Source: ProtInc
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Traceable author statement PubMed 10820279. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Ref.8 Ref.9
	Chain	37 – 114	78	C-X-C motif chemokine 5		PRO_0000005075
	Chain	44 – 114	71	ENA-78(8-78)		PRO_0000005076
	Chain	45 – 114	70	ENA-78(9-78)		PRO_0000005077
Sites
	Site	44 – 45	2	Cleavage; by cathepsin G
Amino acid modifications
	Disulfide bond	49 ↔ 75		By similarity
	Disulfide bond	51 ↔ 91		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42830 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 56B21EE86AE952D3	FASTA	114	11,972
	10 20 30 40 50 60 MSLLSSRAAR VPGPSSSLCA LLVLLLLLTQ PGPIASAGPA AAVLRELRCV CLQTTQGVHP 70 80 90 100 110 KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a full-length cDNA encoding the neutrophil-activating peptide ENA-78 from human platelets." Power C.A., Furness R.B., Brawand C., Wells T.N.C. Gene 151:333-334(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of the human neutrophil-activating peptide (ENA-78) gene." Chang M.S., McNinch J., Basu R., Simonet S. J. Biol. Chem. 269:25277-25282(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of the gene for human neutrophil-activating peptide 78 (ENA-78)." Corbett M.S., Schmitt I., Riess O., Walz A. Biochem. Biophys. Res. Commun. 205:612-617(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus." Zhang C., Thornton M.A., Kowalska M.A., Sachis B.S., Feldman M., Poncz M., McKenzie S.E., Reilly M.P. Blood 98:610-617(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Novel polymorphism in ENA-78 gene." Amoli M.M., Thomson W., Hajeer A.H., Gonzalez-Gay M.A., Ollier W.E.R. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-80.
[7]	"Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to interleukin 8." Walz A., Burgener R., Car B., Baggiolini M., Kunkel S.L., Strieter R.M. J. Exp. Med. 174:1355-1362(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-114. Tissue: Epithelium.
[8]	"Isolation of the CXC chemokines ENA-78, GRO alpha and GRO gamma from tumor cells and leukocytes reveals NH2-terminal heterogeneity. Functional comparison of different natural isoforms." Wuyts A., Govaerts C., Struyf S., Lenaerts J.-P., Put W., Conings R., Proost P., Van Damme J. Eur. J. Biochem. 260:421-429(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-51, IDENTIFICATION OF ENA-78(8-78) AND ENA-78(9-78), PROTEOLYTIC PROCESSING OF N-TERMINUS, FUNCTION. Tissue: Peripheral blood monocyte.
[9]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-45. Tissue: Platelet.
[10]	"Regulation of the immune response by the interaction of chemokines and proteases." Struyf S., Proost P., Van Damme J. Adv. Immunol. 81:1-44(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

Wikipedia

CXCL5 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78686 mRNA. Translation: CAA55355.1. U12709 Genomic DNA. Translation: AAA62475.1. L37036 Genomic DNA. Translation: AAA86426.1. AF349466 Genomic DNA. Translation: AAK29641.1. BC008376 mRNA. Translation: AAH08376.1. AJ315732 Genomic DNA. Translation: CAC42884.1.
IPI	IPI00292936.
PIR	A55010. JC2433.
RefSeq	NP_002985.1. NM_002994.3.
UniGene	Hs.89714.
3D structure databases
ProteinModelPortal	P42830.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5911N.
STRING	9606.ENSP00000296027.
PTM databases
PhosphoSite	P42830.
Polymorphism databases
DMDM	1169525.
Proteomic databases
PaxDb	P42830.
PRIDE	P42830.
Protocols and materials databases
DNASU	6374.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296027; ENSP00000296027; ENSG00000163735.
GeneID	6374.
KEGG	hsa:6374.
UCSC	uc003hhk.3. human.
Organism-specific databases
CTD	6374.
GeneCards	GC04M074851.
HGNC	HGNC:10642. CXCL5.
MIM	600324. gene.
neXtProt	NX_P42830.
PharmGKB	PA35573.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44673.
HOGENOM	HOG000220915.
HOVERGEN	HBG107789.
InParanoid	P42830.
KO	K05506.
OMA	AAGPQCS.
OrthoDB	EOG4NKBX5.
PhylomeDB	P42830.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P42830.
Bgee	P42830.
CleanEx	HS_CXCL5.
Genevestigator	P42830.
GermOnline	ENSG00000163735. Homo sapiens.
Family and domain databases
InterPro	IPR001089. Chemokine_CXC. IPR018048. Chemokine_CXC_CS. IPR001811. Chemokine_IL8-like_dom. [Graphical view]
PANTHER	PTHR10179. PTHR10179. 1 hit.
Pfam	PF00048. IL8. 1 hit. [Graphical view]
PRINTS	PR00437. SMALLCYTKCXC.
SMART	SM00199. SCY. 1 hit. [Graphical view]
SUPFAM	SSF54117. Chemokine_IL8. 1 hit.
PROSITE	PS00471. SMALL_CYTOKINES_CXC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6374.
NextBio	24764.
PMAP-CutDB	P42830.
SOURCE	Search...

Entry information

Entry name

CXCL5_HUMAN

Accession

Primary (citable) accession number: P42830
Secondary accession number(s): Q96QE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.