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P42829 (INHA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH]

EC=1.3.1.9
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene names
Name:inhA
Ordered Locus Names:MSMEG_3151, MSMEI_3070
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.

Catalytic activity

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Enoyl-[acyl-carrier-protein] reductase [NADH]
PRO_0000054913

Regions

Nucleotide binding136 – 16530NAD Potential

Sites

Binding site1581Substrate By similarity

Natural variations

Natural variant941S → A in strain: mc(2)651; INH-resistant.

Sequences

Sequence LengthMass (Da)Tools
P42829 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: AD3BD962D2B78FC6

FASTA26928,527
        10         20         30         40         50         60 
MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI ADRLPKPAPL 

        70         80         90        100        110        120 
LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ SGMGINPFFD APYEDVSKGI 

       130        140        150        160        170        180 
HISAYSYASL AKAVLPIMNP GGGIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG 

       190        200        210        220        230        240 
KVGVRSNLVA AGPIRTLAMS AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK 

       250        260 
TVCALLSDWL PATTGTVIYA DGGASTQLL 

« Hide

References

« Hide 'large scale' references
[1]"inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis."
Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr.
Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02530 Unassigned DNA. Translation: AAC43211.1.
CP000480 Genomic DNA. Translation: ABK73247.1.
CP001663 Genomic DNA. Translation: AFP39534.1.
RefSeqYP_006567829.1. NC_018289.1.
YP_887466.1. NC_008596.1.

3D structure databases

ProteinModelPortalP42829.
SMRP42829. Positions 3-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_3151.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK73247; ABK73247; MSMEG_3151.
AFP39534; AFP39534; MSMEI_3070.
GeneID13425437.
4537137.
KEGGmsg:MSMEI_3070.
msm:MSMEG_3151.
PATRIC18078728. VBIMycSme59918_3112.

Phylogenomic databases

eggNOGCOG0623.
KOK11611.
OMASVAWPVY.
OrthoDBEOG6HF644.
ProtClustDBPRK07889.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-3151-MONOMER.
UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFPIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNetSearch...

Entry information

Entry nameINHA_MYCS2
AccessionPrimary (citable) accession number: P42829
Secondary accession number(s): A0QX28, I7GAK9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 22, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways