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P42829

- INHA_MYCS2

UniProt

P42829 - INHA_MYCS2

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH]

Gene

inhA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei158 – 1581SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi136 – 16530NADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic resistance, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-3151-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] (EC:1.3.1.9)
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:inhA
    Ordered Locus Names:MSMEG_3151, MSMEI_3070
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Enoyl-[acyl-carrier-protein] reductase [NADH]PRO_0000054913Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3151.

    Structurei

    3D structure databases

    ProteinModelPortaliP42829.
    SMRiP42829. Positions 3-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK11611.
    OMAiQHWDKFD.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P42829-1 [UniParc]FASTAAdd to Basket

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    MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI    50
    ADRLPKPAPL LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ 100
    SGMGINPFFD APYEDVSKGI HISAYSYASL AKAVLPIMNP GGGIVGMDFD 150
    PTRAMPAYNW MTVAKSALES VNRFVAREAG KVGVRSNLVA AGPIRTLAMS 200
    AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK TVCALLSDWL 250
    PATTGTVIYA DGGASTQLL 269
    Length:269
    Mass (Da):28,527
    Last modified:November 1, 1995 - v1
    Checksum:iAD3BD962D2B78FC6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti94 – 941S → A in strain: mc(2)651; INH-resistant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02530 Unassigned DNA. Translation: AAC43211.1.
    CP000480 Genomic DNA. Translation: ABK73247.1.
    CP001663 Genomic DNA. Translation: AFP39534.1.
    RefSeqiWP_003894540.1. NC_018289.1.
    YP_006567829.1. NC_018289.1.
    YP_887466.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK73247; ABK73247; MSMEG_3151.
    AFP39534; AFP39534; MSMEI_3070.
    GeneIDi4537137.
    KEGGimsg:MSMEI_3070.
    msm:MSMEG_3151.
    PATRICi18078728. VBIMycSme59918_3112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02530 Unassigned DNA. Translation: AAC43211.1 .
    CP000480 Genomic DNA. Translation: ABK73247.1 .
    CP001663 Genomic DNA. Translation: AFP39534.1 .
    RefSeqi WP_003894540.1. NC_018289.1.
    YP_006567829.1. NC_018289.1.
    YP_887466.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali P42829.
    SMRi P42829. Positions 3-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_3151.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK73247 ; ABK73247 ; MSMEG_3151 .
    AFP39534 ; AFP39534 ; MSMEI_3070 .
    GeneIDi 4537137.
    KEGGi msg:MSMEI_3070.
    msm:MSMEG_3151.
    PATRICi 18078728. VBIMycSme59918_3112.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K11611.
    OMAi QHWDKFD.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci MSME246196:GJ4Y-3151-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis."
      Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr.
      Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiINHA_MYCS2
    AccessioniPrimary (citable) accession number: P42829
    Secondary accession number(s): A0QX28, I7GAK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3