P42829 (INHA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH]
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	inhA
Ordered Locus Names:	MSMEG_3151, MSMEI_3070

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054913
Regions
	Nucleotide binding	136 – 165	30	NAD Potential
Sites
	Binding site	158	1	Substrate By similarity
Natural variations
	Natural variant	94	1	S → A in strain: mc(2)651; INH-resistant.

Sequences

	Sequence		Length	Mass (Da)
	P42829 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AD3BD962D2B78FC6	FASTA	269	28,527
	10 20 30 40 50 60 MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI ADRLPKPAPL 70 80 90 100 110 120 LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ SGMGINPFFD APYEDVSKGI 130 140 150 160 170 180 HISAYSYASL AKAVLPIMNP GGGIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG 190 200 210 220 230 240 KVGVRSNLVA AGPIRTLAMS AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK 250 260 TVCALLSDWL PATTGTVIYA DGGASTQLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis." Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr. Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02530 Unassigned DNA. Translation: AAC43211.1. CP000480 Genomic DNA. Translation: ABK73247.1. CP001663 Genomic DNA. Translation: AFP39534.1.
RefSeq	YP_006567829.1. NC_018289.1. YP_887466.1. NC_008596.1.
3D structure databases
ProteinModelPortal	P42829.
SMR	P42829. Positions 3-269.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_3151.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK73247; ABK73247; MSMEG_3151.
GeneID	13425437. 4537137.
KEGG	msg:MSMEI_3070. msm:MSMEG_3151.
PATRIC	18078728. VBIMycSme59918_3112.
Phylogenomic databases
eggNOG	COG0623.
KO	K11611.
OMA	ISDARAM.
ProtClustDB	PRK07889.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-3151-MONOMER.
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR014358. Enoyl-ACP_Rdtase_NADH. IPR016040. NAD(P)-bd_dom. [Graphical view]
PIRSF	PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INHA_MYCS2

Accession

Primary (citable) accession number: P42829
Secondary accession number(s): A0QX28, I7GAK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program