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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH]

Gene

inhA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway:ifatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi136 – 16530NADSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3151-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC:1.3.1.9)
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:inhA
Ordered Locus Names:MSMEG_3151, MSMEI_3070
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000006158 Componenti: Chromosome UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Enoyl-[acyl-carrier-protein] reductase [NADH]PRO_0000054913Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3151.

Structurei

3D structure databases

ProteinModelPortaliP42829.
SMRiP42829. Positions 3-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK11611.
OMAiDARVIFN.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

Sequencei

Sequence statusi: Complete.

P42829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI
60 70 80 90 100
ADRLPKPAPL LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ
110 120 130 140 150
SGMGINPFFD APYEDVSKGI HISAYSYASL AKAVLPIMNP GGGIVGMDFD
160 170 180 190 200
PTRAMPAYNW MTVAKSALES VNRFVAREAG KVGVRSNLVA AGPIRTLAMS
210 220 230 240 250
AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK TVCALLSDWL
260
PATTGTVIYA DGGASTQLL
Length:269
Mass (Da):28,527
Last modified:November 1, 1995 - v1
Checksum:iAD3BD962D2B78FC6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti94 – 941S → A in strain: mc(2)651; INH-resistant.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02530 Unassigned DNA. Translation: AAC43211.1.
CP000480 Genomic DNA. Translation: ABK73247.1.
CP001663 Genomic DNA. Translation: AFP39534.1.
RefSeqiWP_003894540.1. NZ_CP009494.1.
YP_887466.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73247; ABK73247; MSMEG_3151.
AFP39534; AFP39534; MSMEI_3070.
GeneIDi4537137.
KEGGimsg:MSMEI_3070.
msm:MSMEG_3151.
PATRICi18078728. VBIMycSme59918_3112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02530 Unassigned DNA. Translation: AAC43211.1.
CP000480 Genomic DNA. Translation: ABK73247.1.
CP001663 Genomic DNA. Translation: AFP39534.1.
RefSeqiWP_003894540.1. NZ_CP009494.1.
YP_887466.1. NC_008596.1.

3D structure databases

ProteinModelPortaliP42829.
SMRiP42829. Positions 3-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73247; ABK73247; MSMEG_3151.
AFP39534; AFP39534; MSMEI_3070.
GeneIDi4537137.
KEGGimsg:MSMEI_3070.
msm:MSMEG_3151.
PATRICi18078728. VBIMycSme59918_3112.

Phylogenomic databases

eggNOGiCOG0623.
KOiK11611.
OMAiDARVIFN.
OrthoDBiEOG6HF644.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciMSME246196:GJ4Y-3151-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis."
    Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr.
    Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiINHA_MYCS2
AccessioniPrimary (citable) accession number: P42829
Secondary accession number(s): A0QX28, I7GAK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.