Reviewed, UniProtKB/Swiss-Prot P42829 (INHA_MYCS2)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	inhA
Ordered Locus Names:	MSMEG_3151

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Complete proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054913
Regions
	Nucleotide binding	136 – 165	30	NAD Potential
Sites
	Binding site	158	1	Substrate By similarity
Natural variations
	Natural variant	94	1	S → A in strain: mc(2)651; INH-resistant.

Sequences

	Sequence		Length	Mass (Da)
	P42829-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AD3BD962D2B78FC6	FASTA	269	28,527
	10 20 30 40 50 60 MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI ADRLPKPAPL 70 80 90 100 110 120 LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ SGMGINPFFD APYEDVSKGI 130 140 150 160 170 180 HISAYSYASL AKAVLPIMNP GGGIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG 190 200 210 220 230 240 KVGVRSNLVA AGPIRTLAMS AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK 250 260 TVCALLSDWL PATTGTVIYA DGGASTQLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis." Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr. Science 263:227-230(1994) [PubMed: 8284673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02530 Unassigned DNA. Translation: AAC43211.1. CP000480 Genomic DNA. Translation: ABK73247.1.
RefSeq	YP_887466.1.
3D structure databases
HSSP	HSSP built from PDB template 1BVR based on UniProtKB P46533.
SMR	P42829. Positions 3-269.
ModBase	Search...
Protein-protein interaction databases
STRING	P42829.
Genome annotation databases
GeneID	4537137.
GenomeReviews	Gene locus MSMEG_3151 in contig CP000480_GR.
KEGG	msm:MSMEG_3151.
NMPDR	fig|246196.1.peg.3159.
TIGR	MSMEG_3151.
Phylogenomic databases
OMA	EAGQQMQ.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INHA_MYCS2

Accession

Primary (citable) accession number: P42829
Secondary accession number(s): A0QX28

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)