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P42829

- INHA_MYCS2

UniProt

P42829 - INHA_MYCS2

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH]

Gene

inhA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi136 – 16530NADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3151-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC:1.3.1.9)
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:inhA
Ordered Locus Names:MSMEG_3151, MSMEI_3070
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Enoyl-[acyl-carrier-protein] reductase [NADH]PRO_0000054913Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3151.

Structurei

3D structure databases

ProteinModelPortaliP42829.
SMRiP42829. Positions 3-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK11611.
OMAiQHWDKFD.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

Sequencei

Sequence statusi: Complete.

P42829-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI
60 70 80 90 100
ADRLPKPAPL LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ
110 120 130 140 150
SGMGINPFFD APYEDVSKGI HISAYSYASL AKAVLPIMNP GGGIVGMDFD
160 170 180 190 200
PTRAMPAYNW MTVAKSALES VNRFVAREAG KVGVRSNLVA AGPIRTLAMS
210 220 230 240 250
AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK TVCALLSDWL
260
PATTGTVIYA DGGASTQLL
Length:269
Mass (Da):28,527
Last modified:November 1, 1995 - v1
Checksum:iAD3BD962D2B78FC6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti94 – 941S → A in strain: mc(2)651; INH-resistant.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02530 Unassigned DNA. Translation: AAC43211.1.
CP000480 Genomic DNA. Translation: ABK73247.1.
CP001663 Genomic DNA. Translation: AFP39534.1.
RefSeqiWP_003894540.1. NC_018289.1.
YP_006567829.1. NC_018289.1.
YP_887466.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73247; ABK73247; MSMEG_3151.
AFP39534; AFP39534; MSMEI_3070.
GeneIDi4537137.
KEGGimsm:MSMEG_3151.
PATRICi18078728. VBIMycSme59918_3112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02530 Unassigned DNA. Translation: AAC43211.1 .
CP000480 Genomic DNA. Translation: ABK73247.1 .
CP001663 Genomic DNA. Translation: AFP39534.1 .
RefSeqi WP_003894540.1. NC_018289.1.
YP_006567829.1. NC_018289.1.
YP_887466.1. NC_008596.1.

3D structure databases

ProteinModelPortali P42829.
SMRi P42829. Positions 3-269.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_3151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK73247 ; ABK73247 ; MSMEG_3151 .
AFP39534 ; AFP39534 ; MSMEI_3070 .
GeneIDi 4537137.
KEGGi msm:MSMEG_3151.
PATRICi 18078728. VBIMycSme59918_3112.

Phylogenomic databases

eggNOGi COG0623.
KOi K11611.
OMAi QHWDKFD.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci MSME246196:GJ4Y-3151-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis."
    Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr.
    Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiINHA_MYCS2
AccessioniPrimary (citable) accession number: P42829
Secondary accession number(s): A0QX28, I7GAK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3