P42816 (KPRS_BACCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:

prs

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141107
Regions
	Region	215 – 228	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	132	1	Magnesium Potential
	Metal binding	134	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P42816 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: CD661ABA511DEAED	FASTA	315	34,533
	10 20 30 40 50 60 MSDCQHQLKL FALNSNMKLA KEIAEVMGIE LGKCSVSRFS DGEIQINIEE SIRGDDVFVI 70 80 90 100 110 120 QSTSVPVNEH LMELLIMIDA LKRASARTIN IVMPYYGYAR QDRKARSRNP ITAKLVANLL 130 140 150 160 170 180 ETAGASRVIT LDLHAPQIQG FFDIPIDHLM GVPILADYFK SKQLEDIVVV SPDHGGVTRA 190 200 210 220 230 240 RKLADRLKAP IAIIDKRRPK PNVAEVMNIV GQVAGKTAIL IDDIIDTAGT ITLAANALAE 250 260 270 280 290 300 SGAKEVYACC THPVLSGPAI ERIQSSKIKE LVVTNSIALP EEKKIDKIVK LSVRPLIAEA 310 ITRVYEMKSV SVLFD

References

[1]	"Bacillus caldolyticus prs gene encoding phosphoribosyl-diphosphate synthase." Krath B.N., Hove-Jensen B. Gene 176:73-79(1996) [PubMed: 8918235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 405 / NBRC 15313 / YP-T.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83708 Genomic DNA. Translation: CAA58682.1.
PIR	JC5093.
3D structure databases
ProteinModelPortal	P42816.
SMR	P42816. Positions 8-314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BACCL

Accession

Primary (citable) accession number: P42816

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 31, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program