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P42814

- RNS2_ARATH

UniProt

P42814 - RNS2_ARATH

Protein

Ribonuclease 2

Gene

RNS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    • Comment

    Functioni

    May remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711By similarity
    Active sitei129 – 1291By similarity
    Active sitei133 – 1331By similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: TAIR
    2. ribonuclease T2 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. negative regulation of autophagy Source: TAIR
    2. response to stress Source: UniProtKB-KW
    3. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    4. rRNA catabolic process Source: TAIR

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciARA:AT2G39780-MONOMER.
    ARA:GQT-2593-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 2 (EC:3.1.27.1)
    Gene namesi
    Name:RNS2
    Ordered Locus Names:At2g39780
    ORF Names:T5I7.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G39780.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. ER body Source: TAIR
    3. intracellular Source: TAIR
    4. vacuole Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 259240Ribonuclease 2PRO_0000030967Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi86 ↔ 136By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP42814.
    PRIDEiP42814.

    Expressioni

    Tissue specificityi

    Most highly expressed in flowers, but also expressed in roots, stems, and leaves.

    Inductioni

    By phosphate starvation.

    Gene expression databases

    ArrayExpressiP42814.
    GenevestigatoriP42814.

    Structurei

    3D structure databases

    ProteinModelPortaliP42814.
    SMRiP42814. Positions 31-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNase T2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3719.
    HOGENOMiHOG000238569.
    InParanoidiP42814.
    KOiK01166.
    OMAiWPGTYCR.
    PhylomeDBiP42814.

    Family and domain databases

    Gene3Di3.90.730.10. 1 hit.
    InterProiIPR001568. RNase_T2-like.
    IPR018188. RNase_T2_AS.
    [Graphical view]
    PANTHERiPTHR11240. PTHR11240. 1 hit.
    PfamiPF00445. Ribonuclease_T2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55895. SSF55895. 1 hit.
    PROSITEiPS00530. RNASE_T2_1. 1 hit.
    PS00531. RNASE_T2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P42814-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASRLCLLLL VACIAGAFAG DVIELNRSQR EFDYFALSLQ WPGTYCRGTR    50
    HCCSKNACCR GSDAPTQFTI HGLWPDYNDG SWPSCCYRSD FKEKEISTLM 100
    DGLEKYWPSL SCGSPSSCNG GKGSFWGHEW EKHGTCSSPV FHDEYNYFLT 150
    TLNLYLKHNV TDVLYQAGYV ASNSEKYPLG GIVTAIQNAF HITPEVVCKR 200
    DAIDEIRICF YKDFKPRDCV GSQDLTSRKS CPKYVSLPEY TPLDGEAMVL 250
    KMPTEREAL 259
    Length:259
    Mass (Da):29,153
    Last modified:November 1, 1995 - v1
    Checksum:iFE6DCD6926428942
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti210 – 2101F → S in CAA81330. (PubMed:8580968)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98336 mRNA. Translation: AAA51406.1.
    AC003000 Genomic DNA. Translation: AAB87127.1.
    CP002685 Genomic DNA. Translation: AEC09723.1.
    Z26559 mRNA. Translation: CAA81330.1.
    PIRiT01008.
    RefSeqiNP_030524.1. NM_129536.4. [P42814-1]
    UniGeneiAt.21798.

    Genome annotation databases

    EnsemblPlantsiAT2G39780.1; AT2G39780.1; AT2G39780. [P42814-1]
    GeneIDi818563.
    KEGGiath:AT2G39780.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98336 mRNA. Translation: AAA51406.1 .
    AC003000 Genomic DNA. Translation: AAB87127.1 .
    CP002685 Genomic DNA. Translation: AEC09723.1 .
    Z26559 mRNA. Translation: CAA81330.1 .
    PIRi T01008.
    RefSeqi NP_030524.1. NM_129536.4. [P42814-1 ]
    UniGenei At.21798.

    3D structure databases

    ProteinModelPortali P42814.
    SMRi P42814. Positions 31-239.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P42814.
    PRIDEi P42814.

    Protocols and materials databases

    DNASUi 818563.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G39780.1 ; AT2G39780.1 ; AT2G39780 . [P42814-1 ]
    GeneIDi 818563.
    KEGGi ath:AT2G39780.

    Organism-specific databases

    TAIRi AT2G39780.

    Phylogenomic databases

    eggNOGi COG3719.
    HOGENOMi HOG000238569.
    InParanoidi P42814.
    KOi K01166.
    OMAi WPGTYCR.
    PhylomeDBi P42814.

    Enzyme and pathway databases

    BioCyci ARA:AT2G39780-MONOMER.
    ARA:GQT-2593-MONOMER.

    Gene expression databases

    ArrayExpressi P42814.
    Genevestigatori P42814.

    Family and domain databases

    Gene3Di 3.90.730.10. 1 hit.
    InterProi IPR001568. RNase_T2-like.
    IPR018188. RNase_T2_AS.
    [Graphical view ]
    PANTHERi PTHR11240. PTHR11240. 1 hit.
    Pfami PF00445. Ribonuclease_T2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55895. SSF55895. 1 hit.
    PROSITEi PS00530. RNASE_T2_1. 1 hit.
    PS00531. RNASE_T2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation."
      Taylor C.B., Bariola P.A., Delcardayre S.B., Raines R.T., Green P.J.
      Proc. Natl. Acad. Sci. U.S.A. 90:5118-5122(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-259.
      Strain: cv. Columbia.
      Tissue: Seedling.

    Entry informationi

    Entry nameiRNS2_ARATH
    AccessioniPrimary (citable) accession number: P42814
    Secondary accession number(s): Q42136
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3