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P42814 (RNS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 2

EC=3.1.27.1
Gene names
Name:RNS2
Ordered Locus Names:At2g39780
ORF Names:T5I7.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.

Tissue specificity

Most highly expressed in flowers, but also expressed in roots, stems, and leaves.

Induction

By phosphate starvation.

Sequence similarities

Belongs to the RNase T2 family.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P42814-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 259240Ribonuclease 2
PRO_0000030967

Sites

Active site711 By similarity
Active site1291 By similarity
Active site1331 By similarity

Amino acid modifications

Disulfide bond86 ↔ 136 By similarity

Experimental info

Sequence conflict2101F → S in CAA81330. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: FE6DCD6926428942

FASTA25929,153
        10         20         30         40         50         60 
MASRLCLLLL VACIAGAFAG DVIELNRSQR EFDYFALSLQ WPGTYCRGTR HCCSKNACCR 

        70         80         90        100        110        120 
GSDAPTQFTI HGLWPDYNDG SWPSCCYRSD FKEKEISTLM DGLEKYWPSL SCGSPSSCNG 

       130        140        150        160        170        180 
GKGSFWGHEW EKHGTCSSPV FHDEYNYFLT TLNLYLKHNV TDVLYQAGYV ASNSEKYPLG 

       190        200        210        220        230        240 
GIVTAIQNAF HITPEVVCKR DAIDEIRICF YKDFKPRDCV GSQDLTSRKS CPKYVSLPEY 

       250 
TPLDGEAMVL KMPTEREAL 

« Hide

References

« Hide 'large scale' references
[1]"RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation."
Taylor C.B., Bariola P.A., Delcardayre S.B., Raines R.T., Green P.J.
Proc. Natl. Acad. Sci. U.S.A. 90:5118-5122(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-259.
Strain: cv. Columbia.
Tissue: Seedling.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98336 mRNA. Translation: AAA51406.1.
AC003000 Genomic DNA. Translation: AAB87127.1.
CP002685 Genomic DNA. Translation: AEC09723.1.
Z26559 mRNA. Translation: CAA81330.1.
PIRT01008.
RefSeqNP_030524.1. NM_129536.4.
UniGeneAt.21798.

3D structure databases

ProteinModelPortalP42814.
SMRP42814. Positions 31-239.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP42814.
PRIDEP42814.

Protocols and materials databases

DNASU818563.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G39780.1; AT2G39780.1; AT2G39780. [P42814-1]
GeneID818563.
KEGGath:AT2G39780.

Organism-specific databases

TAIRAT2G39780.

Phylogenomic databases

eggNOGCOG3719.
HOGENOMHOG000238569.
InParanoidP42814.
KOK01166.
OMACRADDDS.
PhylomeDBP42814.
ProtClustDBCLSN2688811.

Enzyme and pathway databases

BioCycARA:AT2G39780-MONOMER.
ARA:GQT-2593-MONOMER.

Gene expression databases

ArrayExpressP42814.
GenevestigatorP42814.

Family and domain databases

Gene3D3.90.730.10. 1 hit.
InterProIPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]
PANTHERPTHR11240. PTHR11240. 1 hit.
PfamPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMSSF55895. SSF55895. 1 hit.
PROSITEPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNS2_ARATH
AccessionPrimary (citable) accession number: P42814
Secondary accession number(s): Q42136
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names