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Protein

Ribonuclease 2

Gene

RNS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711By similarity
Active sitei129 – 1291By similarity
Active sitei133 – 1331By similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: TAIR
  2. ribonuclease T2 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro

GO - Biological processi

  1. negative regulation of autophagy Source: TAIR
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. rRNA catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciARA:AT2G39780-MONOMER.
ARA:GQT-2593-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 2 (EC:3.1.27.1)
Gene namesi
Name:RNS2
Ordered Locus Names:At2g39780
ORF Names:T5I7.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39780.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. ER body Source: TAIR
  3. intracellular Source: TAIR
  4. vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 259240Ribonuclease 2PRO_0000030967Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi86 ↔ 136By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP42814.
PRIDEiP42814.

Expressioni

Tissue specificityi

Most highly expressed in flowers, but also expressed in roots, stems, and leaves.

Inductioni

By phosphate starvation.

Gene expression databases

ExpressionAtlasiP42814. baseline and differential.
GenevestigatoriP42814.

Structurei

3D structure databases

ProteinModelPortaliP42814.
SMRiP42814. Positions 31-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3719.
HOGENOMiHOG000238569.
InParanoidiP42814.
KOiK01166.
OMAiCRADDDS.
PhylomeDBiP42814.

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P42814-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRLCLLLL VACIAGAFAG DVIELNRSQR EFDYFALSLQ WPGTYCRGTR
60 70 80 90 100
HCCSKNACCR GSDAPTQFTI HGLWPDYNDG SWPSCCYRSD FKEKEISTLM
110 120 130 140 150
DGLEKYWPSL SCGSPSSCNG GKGSFWGHEW EKHGTCSSPV FHDEYNYFLT
160 170 180 190 200
TLNLYLKHNV TDVLYQAGYV ASNSEKYPLG GIVTAIQNAF HITPEVVCKR
210 220 230 240 250
DAIDEIRICF YKDFKPRDCV GSQDLTSRKS CPKYVSLPEY TPLDGEAMVL

KMPTEREAL
Length:259
Mass (Da):29,153
Last modified:October 31, 1995 - v1
Checksum:iFE6DCD6926428942
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101F → S in CAA81330 (PubMed:8580968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98336 mRNA. Translation: AAA51406.1.
AC003000 Genomic DNA. Translation: AAB87127.1.
CP002685 Genomic DNA. Translation: AEC09723.1.
Z26559 mRNA. Translation: CAA81330.1.
PIRiT01008.
RefSeqiNP_030524.1. NM_129536.4. [P42814-1]
UniGeneiAt.21798.

Genome annotation databases

EnsemblPlantsiAT2G39780.1; AT2G39780.1; AT2G39780. [P42814-1]
GeneIDi818563.
KEGGiath:AT2G39780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98336 mRNA. Translation: AAA51406.1.
AC003000 Genomic DNA. Translation: AAB87127.1.
CP002685 Genomic DNA. Translation: AEC09723.1.
Z26559 mRNA. Translation: CAA81330.1.
PIRiT01008.
RefSeqiNP_030524.1. NM_129536.4. [P42814-1]
UniGeneiAt.21798.

3D structure databases

ProteinModelPortaliP42814.
SMRiP42814. Positions 31-239.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP42814.
PRIDEiP42814.

Protocols and materials databases

DNASUi818563.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39780.1; AT2G39780.1; AT2G39780. [P42814-1]
GeneIDi818563.
KEGGiath:AT2G39780.

Organism-specific databases

TAIRiAT2G39780.

Phylogenomic databases

eggNOGiCOG3719.
HOGENOMiHOG000238569.
InParanoidiP42814.
KOiK01166.
OMAiCRADDDS.
PhylomeDBiP42814.

Enzyme and pathway databases

BioCyciARA:AT2G39780-MONOMER.
ARA:GQT-2593-MONOMER.

Gene expression databases

ExpressionAtlasiP42814. baseline and differential.
GenevestigatoriP42814.

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation."
    Taylor C.B., Bariola P.A., Delcardayre S.B., Raines R.T., Green P.J.
    Proc. Natl. Acad. Sci. U.S.A. 90:5118-5122(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-259.
    Strain: cv. Columbia.
    Tissue: Seedling.

Entry informationi

Entry nameiRNS2_ARATH
AccessioniPrimary (citable) accession number: P42814
Secondary accession number(s): Q42136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: January 6, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.