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P42814

- RNS2_ARATH

UniProt

P42814 - RNS2_ARATH

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Protein
Ribonuclease 2
Gene
RNS2, At2g39780, T5I7.8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

May remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711 By similarity
Active sitei129 – 1291 By similarity
Active sitei133 – 1331 By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: TAIR
  3. ribonuclease T2 activity Source: UniProtKB-EC

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  2. negative regulation of autophagy Source: TAIR
  3. rRNA catabolic process Source: TAIR
  4. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciARA:AT2G39780-MONOMER.
ARA:GQT-2593-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 2 (EC:3.1.27.1)
Gene namesi
Name:RNS2
Ordered Locus Names:At2g39780
ORF Names:T5I7.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G39780.

Subcellular locationi

GO - Cellular componenti

  1. ER body Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. intracellular Source: TAIR
  4. vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 259240Ribonuclease 2
PRO_0000030967Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi86 ↔ 136 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP42814.
PRIDEiP42814.

Expressioni

Tissue specificityi

Most highly expressed in flowers, but also expressed in roots, stems, and leaves.

Inductioni

By phosphate starvation.

Gene expression databases

ArrayExpressiP42814.
GenevestigatoriP42814.

Structurei

3D structure databases

ProteinModelPortaliP42814.
SMRiP42814. Positions 31-239.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3719.
HOGENOMiHOG000238569.
InParanoidiP42814.
KOiK01166.
OMAiWPGTYCR.
PhylomeDBiP42814.

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P42814-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASRLCLLLL VACIAGAFAG DVIELNRSQR EFDYFALSLQ WPGTYCRGTR    50
HCCSKNACCR GSDAPTQFTI HGLWPDYNDG SWPSCCYRSD FKEKEISTLM 100
DGLEKYWPSL SCGSPSSCNG GKGSFWGHEW EKHGTCSSPV FHDEYNYFLT 150
TLNLYLKHNV TDVLYQAGYV ASNSEKYPLG GIVTAIQNAF HITPEVVCKR 200
DAIDEIRICF YKDFKPRDCV GSQDLTSRKS CPKYVSLPEY TPLDGEAMVL 250
KMPTEREAL 259
Length:259
Mass (Da):29,153
Last modified:November 1, 1995 - v1
Checksum:iFE6DCD6926428942
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101F → S in CAA81330. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98336 mRNA. Translation: AAA51406.1.
AC003000 Genomic DNA. Translation: AAB87127.1.
CP002685 Genomic DNA. Translation: AEC09723.1.
Z26559 mRNA. Translation: CAA81330.1.
PIRiT01008.
RefSeqiNP_030524.1. NM_129536.4. [P42814-1]
UniGeneiAt.21798.

Genome annotation databases

EnsemblPlantsiAT2G39780.1; AT2G39780.1; AT2G39780. [P42814-1]
GeneIDi818563.
KEGGiath:AT2G39780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98336 mRNA. Translation: AAA51406.1 .
AC003000 Genomic DNA. Translation: AAB87127.1 .
CP002685 Genomic DNA. Translation: AEC09723.1 .
Z26559 mRNA. Translation: CAA81330.1 .
PIRi T01008.
RefSeqi NP_030524.1. NM_129536.4. [P42814-1 ]
UniGenei At.21798.

3D structure databases

ProteinModelPortali P42814.
SMRi P42814. Positions 31-239.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P42814.
PRIDEi P42814.

Protocols and materials databases

DNASUi 818563.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G39780.1 ; AT2G39780.1 ; AT2G39780 . [P42814-1 ]
GeneIDi 818563.
KEGGi ath:AT2G39780.

Organism-specific databases

TAIRi AT2G39780.

Phylogenomic databases

eggNOGi COG3719.
HOGENOMi HOG000238569.
InParanoidi P42814.
KOi K01166.
OMAi WPGTYCR.
PhylomeDBi P42814.

Enzyme and pathway databases

BioCyci ARA:AT2G39780-MONOMER.
ARA:GQT-2593-MONOMER.

Gene expression databases

ArrayExpressi P42814.
Genevestigatori P42814.

Family and domain databases

Gene3Di 3.90.730.10. 1 hit.
InterProi IPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view ]
PANTHERi PTHR11240. PTHR11240. 1 hit.
Pfami PF00445. Ribonuclease_T2. 1 hit.
[Graphical view ]
SUPFAMi SSF55895. SSF55895. 1 hit.
PROSITEi PS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation."
    Taylor C.B., Bariola P.A., Delcardayre S.B., Raines R.T., Green P.J.
    Proc. Natl. Acad. Sci. U.S.A. 90:5118-5122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-259.
    Strain: cv. Columbia.
    Tissue: Seedling.

Entry informationi

Entry nameiRNS2_ARATH
AccessioniPrimary (citable) accession number: P42814
Secondary accession number(s): Q42136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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