P42814 (RNS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 2
EC=3.1.27.1

Gene names

Name:	RNS2
Ordered Locus Names:	At2g39780
ORF Names:	T5I7.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.
Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Tissue specificity	Most highly expressed in flowers, but also expressed in roots, stems, and leaves.
Induction	By phosphate starvation.
Sequence similarities	Belongs to the RNase T2 family.

Ontologies

Keywords
Biological process	Stress response
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of autophagy Inferred from mutant phenotype PubMed 21199950. Source: TAIR rRNA catabolic process Inferred from mutant phenotype PubMed 21199950. Source: TAIR response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER body Inferred from direct assay PubMed 21199950. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 21199950. Source: TAIR vacuole Inferred from direct assay PubMed 15539469PubMed 21199950. Source: TAIR
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from direct assay PubMed 21199950. Source: TAIR ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P42814-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 259	240	Ribonuclease 2		PRO_0000030967
Sites
	Active site	71	1	By similarity
	Active site	129	1	By similarity
	Active site	133	1	By similarity
Amino acid modifications
	Disulfide bond	86 ↔ 136		By similarity
Experimental info
	Sequence conflict	210	1	F → S in CAA81330. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FE6DCD6926428942	FASTA	259	29,153
	10 20 30 40 50 60 MASRLCLLLL VACIAGAFAG DVIELNRSQR EFDYFALSLQ WPGTYCRGTR HCCSKNACCR 70 80 90 100 110 120 GSDAPTQFTI HGLWPDYNDG SWPSCCYRSD FKEKEISTLM DGLEKYWPSL SCGSPSSCNG 130 140 150 160 170 180 GKGSFWGHEW EKHGTCSSPV FHDEYNYFLT TLNLYLKHNV TDVLYQAGYV ASNSEKYPLG 190 200 210 220 230 240 GIVTAIQNAF HITPEVVCKR DAIDEIRICF YKDFKPRDCV GSQDLTSRKS CPKYVSLPEY 250 TPLDGEAMVL KMPTEREAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation." Taylor C.B., Bariola P.A., Delcardayre S.B., Raines R.T., Green P.J. Proc. Natl. Acad. Sci. U.S.A. 90:5118-5122(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 197-259. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98336 mRNA. Translation: AAA51406.1. AC003000 Genomic DNA. Translation: AAB87127.1. CP002685 Genomic DNA. Translation: AEC09723.1. Z26559 mRNA. Translation: CAA81330.1.
IPI	IPI00539416.
PIR	T01008.
RefSeq	NP_030524.1. NM_129536.4.
UniGene	At.21798.
3D structure databases
ProteinModelPortal	P42814.
SMR	P42814. Positions 31-239.
ModBase	Search...
Proteomic databases
PaxDb	P42814.
PRIDE	P42814.
Protocols and materials databases
DNASU	818563.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G39780.1; AT2G39780.1; AT2G39780.
GeneID	818563.
KEGG	ath:AT2G39780.
Organism-specific databases
TAIR	At2g39780.
Phylogenomic databases
eggNOG	COG3719.
HOGENOM	HOG000238569.
InParanoid	P42814.
KO	K01166.
OMA	SELAYFT.
PhylomeDB	P42814.
ProtClustDB	CLSN2688811.
Gene expression databases
ArrayExpress	P42814.
Genevestigator	P42814.
GermOnline	AT2G39780. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.90.730.10. 1 hit.
InterPro	IPR001568. RNase_T2-like. IPR018188. RNase_T2_AS. [Graphical view]
PANTHER	PTHR11240. PTHR11240. 1 hit.
Pfam	PF00445. Ribonuclease_T2. 1 hit. [Graphical view]
SUPFAM	SSF55895. RNase_T2. 1 hit.
PROSITE	PS00530. RNASE_T2_1. 1 hit. PS00531. RNASE_T2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNS2_ARATH

Accession

Primary (citable) accession number: P42814
Secondary accession number(s): Q42136

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program