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P42809 (HEM1_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-tRNA reductase
Short name=GluTR
EC=1.2.1.70
Gene names
Name:hemA
Ordered Locus Names:MTBMA_c13940
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter ›

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). Ref.1

Catalytic activity

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. Ref.1

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. HAMAP-Rule MF_00087

Subunit structure

Homodimer By similarity.

Domain

Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization By similarity. HAMAP-Rule MF_00087

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. HAMAP-Rule MF_00087

Sequence similarities

Belongs to the glutamyl-tRNA reductase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0. Ref.1

Temperature dependence:

Optimum temperature is 65 degrees Celsius.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processchlorophyll biosynthetic process

Inferred from electronic annotation. Source: HAMAP

protoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA reductase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Glutamyl-tRNA reductase HAMAP-Rule MF_00087
PRO_0000114106

Regions

Nucleotide binding168 – 1736NADP By similarity
Region45 – 484Substrate binding By similarity
Region93 – 953Substrate binding By similarity

Sites

Active site461Nucleophile By similarity
Binding site881Substrate By similarity
Binding site991Substrate By similarity
Site781Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P42809 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7A2C457D9E48CB67

FASTA39844,536
        10         20         30         40         50         60 
MILNIRLDHK TSDVKTMETA SGRIEEIVGE LEALGAVTEK VPLMTCNRVE YYLHVTGVPP 

        70         80         90        100        110        120 
EFDFNGFTVE KDEDALLHLL RLASGLESMI IGEDQILGQI KAARLQALRE GTCGPLLDMV 

       130        140        150        160        170        180 
FTKAVHVGQT VRRKTKINRG SVSIGSAAVD LAESIHGDLK CKKVLVIGAG KMGTLVARAL 

       190        200        210        220        230        240 
AEKHLKAIMV ANRTYERAYQ LACELGGDAI HFDRLNRALR DADVVISATG SPHYILTRER 

       250        260        270        280        290        300 
VMDAVPPERR SSIVMVDIAN PRDIEESVRE LGVRLFTIDD LRGVAEENRK RREAEAREAE 

       310        320        330        340        350        360 
GIVRAELELL LRAMKHREVE PLLAEIRGRM ESLRQREAGK AIKKIENSKD PERVVEGLTR 

       370        380        390 
SIVDKIFHDI ALKIRDAAER DDREFLRMCS ELFDCDES

« Hide

References

« Hide 'large scale' references
[1]"The hemA gene encoding glutamyl-tRNA reductase from the archaeon Methanobacterium thermoautotrophicum strain Marburg."
Hungerer C., Weiss D.S., Thauer R.K., Jahn D.
Bioorg. Med. Chem. 4:1089-1095(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X83691 Genomic DNA. Translation: CAA58664.1.
CP001710 Genomic DNA. Translation: ADL58981.1.
PIRS51136.
RefSeqYP_003850294.1. NC_014408.1.

3D structure databases

ProteinModelPortalP42809.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL58981; ADL58981; MTBMA_c13940.
GeneID9705103.
KEGGmmg:MTBMA_c13940.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000109651.
KOK02492.
OMAKCVLINT.
ProtClustDBPRK00045.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-1402-MONOMER.
UniPathwayUPA00251; UER00316.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00087. Glu-tRNA_reductase.
InterProIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR016040. NAD(P)-bd_dom.
IPR018214. Pyrrol_synth_GluRdtase_CS.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
PIRSFPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMSSF69075. 4pyrrol_synth_GluRdtase_C. 1 hit.
SSF69742. GlutR. 1 hit.
TIGRFAMsTIGR01035. hemA. 1 hit.
PROSITEPS00747. GLUTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM1_METTM
AccessionPrimary (citable) accession number: P42809
Secondary accession number(s): D9PXN3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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