Reviewed,
UniProtKB/Swiss-Prot P42804 (HEM11_ARATH)
Last modified
November 25, 2008.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutamyl-tRNA reductase 1, chloroplastic Short name=GluTR EC=1.2.1.70 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 543 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. |
| Catalytic activity | L-glutamate 1-semialdehyde + NADP(+) + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. |
| Pathway | |
| Subcellular location | |
| Tissue specificity | Present in all tissues tested. Slightly more abundant in leaves. |
| Induction | By light. |
| Miscellaneous | During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. |
| Sequence similarities | Belongs to the glutamyl-tRNA reductase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Chlorophyll biosynthesis Porphyrin biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro glutamyl-tRNA reductase activityInferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Chloroplast Potential | |||||||
| Chain | ? – 543 | Glutamyl-tRNA reductase 1, chloroplastic | PRO_0000013307 | ||||||
Regions | |||||||||
| Nucleotide binding | 285 – 290 | 6 | NADP By similarity | ||||||
| Region | 143 – 146 | 4 | Substrate binding By similarity | ||||||
| Region | 208 – 210 | 3 | Substrate binding By similarity | ||||||
| Compositional bias | 23 – 33 | 11 | Poly-Ser | ||||||
| Compositional bias | 305 – 308 | 4 | Poly-Val | ||||||
Sites | |||||||||
| Active site | 144 | 1 | Nucleophile By similarity | ||||||
| Binding site | 203 | 1 | Substrate By similarity | ||||||
| Binding site | 214 | 1 | Substrate By similarity | ||||||
| Site | 193 | 1 | Important for activity By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 62 | 1 | E → V in AAA19118. Ref.1 | ||||||
| Sequence conflict | 169 | 1 | K → N in AAA19118. Ref.1 | ||||||
| Sequence conflict | 291 | 1 | K → L in AAA19118. Ref.1 | ||||||
| Sequence conflict | 542 | 1 | Q → H in AAA19118. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis." Ilag L.L., Kumar A.M., Soell D. Plant Cell 6:265-275(1994) [PubMed: 7908550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia. Tissue: Leaf. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| U03774 Genomic DNA. Translation: AAA19118.1. AC008051 Genomic DNA. Translation: AAF82258.1. AY072223 mRNA. Translation: AAL60044.1. AY096600 mRNA. Translation: AAM20250.1. | |
| PIR | E96616. |
| RefSeq | NP_176125.1. |
| UniGene | At.241 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GPJ based on UniProtKB Q9UXR8. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 842198. |
| GenomeReviews | Gene locus AT1G58290 in contig CT485782_GR. |
| KEGG | ath:AT1G58290. |
| NMPDR | fig|3702.1.peg.5290. |
Organism-specific databases | |
| TAIR | At1g58290. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT1G58290-MON. |
Gene expression databases | |
| ArrayExpress | P42804. |
| GermOnline | AT1G58290. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR000343. 4pyrrol_synth_GluRdtase. IPR015896. 4pyrrol_synth_GluRdtase_C. IPR015895. 4pyrrol_synth_GluRdtase_N. IPR016040. NAD(P)-bd. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00745. GlutR_dimer. 1 hit. PF05201. GlutR_N. 1 hit. PF01488. Shikimate_DH. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01035. hemA. 1 hit. |
| PROSITE | PS00747. GLUTR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HEM11_ARATH | ||||||||
| Accession | Primary (citable) accession number: P42804 Secondary accession number(s): Q9LQB9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


