P42804 (HEM11_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 115.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glutamyl-tRNA reductase 1, chloroplastic Short name=GluTR EC=1.2.1.70 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis![]() |
Protein attributes
| Sequence length | 543 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. |
| Catalytic activity | L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. |
| Pathway | |
| Subunit structure | Interacts (via C-terminus) with FLU. Ref.5 |
| Subcellular location | |
| Tissue specificity | Present in all tissues tested. Slightly more abundant in leaves. Ref.1 |
| Induction | By light. Ref.1 |
| Miscellaneous | During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. |
| Sequence similarities | Belongs to the glutamyl-tRNA reductase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Chlorophyll biosynthesis Porphyrin biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | chlorophyll biosynthetic process Inferred from mutant phenotype PubMed 10631248. Source: TAIR heme biosynthetic processInferred from mutant phenotype PubMed 10631248. Source: TAIR protoporphyrinogen IX biosynthetic processInferred from electronic annotation. Source: UniProtKB-UniPathway |
| Cellular_component | chloroplast Inferred from direct assay PubMed 15028209. Source: TAIR |
| Molecular_function | NADP binding Inferred from electronic annotation. Source: InterPro glutamyl-tRNA reductase activityTraceable author statement PubMed 10631248. Source: TAIR |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 64 | 64 | Chloroplast Potential | ||||||
| Chain | 65 – 543 | 479 | Glutamyl-tRNA reductase 1, chloroplastic | PRO_0000013307 | |||||
Regions | |||||||||
| Nucleotide binding | 285 – 290 | 6 | NADP By similarity | ||||||
| Region | 143 – 146 | 4 | Substrate binding By similarity | ||||||
| Region | 208 – 210 | 3 | Substrate binding By similarity | ||||||
| Compositional bias | 23 – 33 | 11 | Poly-Ser | ||||||
| Compositional bias | 305 – 308 | 4 | Poly-Val | ||||||
Sites | |||||||||
| Active site | 144 | 1 | Nucleophile By similarity | ||||||
| Binding site | 203 | 1 | Substrate By similarity | ||||||
| Binding site | 214 | 1 | Substrate By similarity | ||||||
| Site | 193 | 1 | Important for activity By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 62 | 1 | E → V in AAA19118. Ref.1 | ||||||
| Sequence conflict | 169 | 1 | K → N in AAA19118. Ref.1 | ||||||
| Sequence conflict | 291 | 1 | K → L in AAA19118. Ref.1 | ||||||
| Sequence conflict | 542 | 1 | Q → H in AAA19118. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis." Ilag L.L., Kumar A.M., Soell D. Plant Cell 6:265-275(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia. Tissue: Leaf. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Concurrent interactions of heme and FLU with Glu tRNA reductase (HEMA1), the target of metabolic feedback inhibition of tetrapyrrole biosynthesis, in dark- and light-grown Arabidopsis plants." Goslings D., Meskauskiene R., Kim C., Lee K.P., Nater M., Apel K. Plant J. 40:957-967(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FLU. Strain: cv. Landsberg erecta. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U03774 Genomic DNA. Translation: AAA19118.1. AC008051 Genomic DNA. Translation: AAF82258.1. CP002684 Genomic DNA. Translation: AEE33532.1. AY072223 mRNA. Translation: AAL60044.1. AY096600 mRNA. Translation: AAM20250.1. |
| IPI | IPI00535419. |
| PIR | E96616. |
| RefSeq | NP_176125.1. NM_104609.3. |
| UniGene | At.241. |
3D structure databases | |
| ProteinModelPortal | P42804. |
| SMR | P42804. Positions 97-468. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P42804. 4 interactions. |
Proteomic databases | |
| PaxDb | P42804. |
| PRIDE | P42804. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT1G58290.1; AT1G58290.1; AT1G58290. |
| GeneID | 842198. |
| KEGG | ath:AT1G58290. |
Organism-specific databases | |
| TAIR | At1g58290. |
Phylogenomic databases | |
| eggNOG | COG0373. |
| HOGENOM | HOG000109651. |
| InParanoid | P42804. |
| KO | K02492. |
| OMA | GPILNRL. |
| PhylomeDB | P42804. |
| ProtClustDB | PLN00203. |
Enzyme and pathway databases | |
| BioCyc | ARA:AT1G58290-MONOMER. MetaCyc:AT1G58290-MONOMER. |
| UniPathway | UPA00251; UER00316. |
Gene expression databases | |
| ArrayExpress | P42804. |
| Genevestigator | P42804. |
| GermOnline | AT1G58290. Arabidopsis thaliana. |
Family and domain databases | |
| Gene3D | 3.40.50.720. 1 hit. |
| InterPro | IPR000343. 4pyrrol_synth_GluRdtase. IPR015896. 4pyrrol_synth_GluRdtase_dimer. IPR015895. 4pyrrol_synth_GluRdtase_N. IPR016040. NAD(P)-bd_dom. IPR018214. Pyrrol_synth_GluRdtase_CS. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view] |
| Pfam | PF00745. GlutR_dimer. 1 hit. PF05201. GlutR_N. 1 hit. PF01488. Shikimate_DH. 1 hit. [Graphical view] |
| SUPFAM | SSF69075. 4pyrrol_synth_GluRdtase_C. 1 hit. SSF69742. GlutR. 1 hit. |
| TIGRFAMs | TIGR01035. hemA. 1 hit. |
| PROSITE | PS00747. GLUTR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HEM11_ARATH | ||||||||
| Accession | Primary (citable) accession number: P42804 Secondary accession number(s): Q9LQB9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
