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P42799 (GSA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic

Short name=GSA 1
EC=5.4.3.8
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 1
Short name=GSA-AT 1
Gene names
Name:GSA1
Synonyms:HEML1
Ordered Locus Names:At5g63570
ORF Names:MBK5.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transaminase converting glutamate 1-semialdehyde (GSA) to 5-aminolevulinate (ALA). Involved in the biosynthesis of tetrapyrroles. Ref.1

Catalytic activity

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375

Porphyrin-containing compound metabolism; chlorophyll biosynthesis. HAMAP-Rule MF_00375

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00375

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00375.

Tissue specificity

Present in all tissues tested. Ref.1

Induction

By light. In etiolated seedlings, initial expression is reduced but after further illumination, levels steadily increase. Ref.1

Sequence similarities

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Chloroplast By similarity
Chain39 – 474436Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic HAMAP-Rule MF_00375
PRO_0000001255

Amino acid modifications

Modified residue3141N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P42799 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0D5E1A84F2B7433E

FASTA47450,370
        10         20         30         40         50         60 
MSATLTGSGT ALGFSCSSKI SKRVSSSPAS NRCCIKMSVS VDEKKKSFSL QKSEEAFNAA 

        70         80         90        100        110        120 
KNLMPGGVNS PVRAFKSVGG QPVLIDSVKG SKMWDIDGNE YIDYVGSWGP AIIGHADDEV 

       130        140        150        160        170        180 
LAALAETMKK GTSFGAPCLL ENVLAEMVIS AVPSIEMVRF VNSGTEACMG VLRLARAFTN 

       190        200        210        220        230        240 
KEKFIKFEGC YHGHANAFLV KAGSGVATLG LPDSPGVPKA ATSDTLTAPY NDLEAVEKLF 

       250        260        270        280        290        300 
AAHKGEISAV ILEPVVGNSG FIPPTPEFIN GLRQLTKDNG VLLIFDEVMT GFRLAYGGAQ 

       310        320        330        340        350        360 
EYFGITPDLT TLGKIIGGGL PVGAYGGRRD IMEMVAPAGP MYQAGTLSGN PLAMTAGIHT 

       370        380        390        400        410        420 
LKRLKQAGTY EYLDKITKEL TNGILEAGKK TGHPMCGGYI SGMFGFFFAE GPVYNFADSK 

       430        440        450        460        470 
KSDTEKFGRF FRGMLEEGVY FAPSQFEAGF TSLAHTPEDI QLTIAAAERV LSRI 

« Hide

References

« Hide 'large scale' references
[1]"Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis."
Ilag L.L., Kumar A.M., Soell D.
Plant Cell 6:265-275(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION BY LIGHT, TISSUE SPECIFICITY.
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03773 Genomic DNA. Translation: AAA19117.1.
AB005234 Genomic DNA. Translation: BAB10450.1.
CP002688 Genomic DNA. Translation: AED97770.1.
AY102109 mRNA. Translation: AAM26679.1.
AY139804 mRNA. Translation: AAM98110.1.
RefSeqNP_201162.1. NM_125752.3.
UniGeneAt.27758.

3D structure databases

ProteinModelPortalP42799.
SMRP42799. Positions 52-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21718. 1 interaction.
IntActP42799. 1 interaction.
STRING3702.AT5G63570.1-P.

Proteomic databases

PaxDbP42799.
PRIDEP42799.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G63570.1; AT5G63570.1; AT5G63570.
GeneID836476.
KEGGath:AT5G63570.

Organism-specific databases

TAIRAT5G63570.

Phylogenomic databases

eggNOGCOG0001.
HOGENOMHOG000020210.
InParanoidP42799.
KOK01845.
OMAPGFKPDI.
PhylomeDBP42799.
ProtClustDBPLN02482.

Enzyme and pathway databases

BioCycARA:AT5G63570-MONOMER.
MetaCyc:AT5G63570-MONOMER.
UniPathwayUPA00251; UER00317.
UPA00668.

Gene expression databases

GenevestigatorP42799.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPMF_00375. HemL_aminotrans_3.
InterProIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERPTHR11986. PTHR11986. 1 hit.
PfamPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR00713. hemL. 1 hit.
PROSITEPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSA1_ARATH
AccessionPrimary (citable) accession number: P42799
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names