Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic

Gene

GSA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transaminase converting glutamate 1-semialdehyde (GSA) to 5-aminolevulinate (ALA). Involved in the biosynthesis of tetrapyrroles.1 Publication

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At2g27450), Glutamyl-tRNA reductase (AXX17_At1g52270), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT5G63570-MONOMER
MetaCyc:AT5G63570-MONOMER
UniPathwayiUPA00251; UER00317
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (EC:5.4.3.8)
Short name:
GSA 1
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 1
Short name:
GSA-AT 1
Gene namesi
Name:GSA1
Synonyms:HEML1
Ordered Locus Names:At5g63570
ORF Names:MBK5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63570
TAIRilocus:2160554 AT5G63570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastBy similarityAdd BLAST38
ChainiPRO_000000125539 – 474Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplasticAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP42799
PRIDEiP42799

Expressioni

Tissue specificityi

Present in all tissues tested.1 Publication

Inductioni

By light. In etiolated seedlings, initial expression is reduced but after further illumination, levels steadily increase.1 Publication

Gene expression databases

ExpressionAtlasiP42799 baseline and differential
GenevisibleiP42799 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi21718, 2 interactors
IntActiP42799, 1 interactor
STRINGi3702.AT5G63570.1

Structurei

Secondary structure

1474
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 60Combined sources10
Turni61 – 63Combined sources3
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Helixi71 – 74Combined sources4
Helixi76 – 78Combined sources3
Beta strandi85 – 90Combined sources6
Beta strandi92 – 95Combined sources4
Beta strandi100 – 105Combined sources6
Helixi106 – 108Combined sources3
Turni109 – 113Combined sources5
Helixi118 – 128Combined sources11
Helixi139 – 151Combined sources13
Beta strandi156 – 163Combined sources8
Helixi164 – 179Combined sources16
Beta strandi183 – 188Combined sources6
Helixi196 – 198Combined sources3
Beta strandi205 – 208Combined sources4
Beta strandi212 – 214Combined sources3
Helixi219 – 222Combined sources4
Beta strandi225 – 229Combined sources5
Helixi233 – 242Combined sources10
Turni244 – 246Combined sources3
Beta strandi247 – 252Combined sources6
Beta strandi254 – 256Combined sources3
Helixi266 – 278Combined sources13
Beta strandi282 – 286Combined sources5
Turni288 – 293Combined sources6
Helixi298 – 303Combined sources6
Beta strandi308 – 313Combined sources6
Helixi314 – 317Combined sources4
Beta strandi323 – 327Combined sources5
Helixi329 – 332Combined sources4
Turni336 – 338Combined sources3
Beta strandi339 – 341Combined sources3
Helixi351 – 364Combined sources14
Helixi369 – 390Combined sources22
Beta strandi396 – 400Combined sources5
Beta strandi403 – 411Combined sources9
Helixi416 – 419Combined sources4
Helixi424 – 436Combined sources13
Helixi457 – 471Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HDMX-ray1.25A/B41-474[»]
ProteinModelPortaliP42799
SMRiP42799
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IPFV Eukaryota
COG0001 LUCA
HOGENOMiHOG000020210
InParanoidiP42799
KOiK01845
OMAiWGPLIFG
OrthoDBiEOG093608WJ
PhylomeDBiP42799

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00375 HemL_aminotrans_3, 1 hit
InterProiView protein in InterPro
IPR004639 4pyrrol_synth_GluAld_NH2Trfase
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00713 hemL, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATLTGSGT ALGFSCSSKI SKRVSSSPAS NRCCIKMSVS VDEKKKSFSL
60 70 80 90 100
QKSEEAFNAA KNLMPGGVNS PVRAFKSVGG QPVLIDSVKG SKMWDIDGNE
110 120 130 140 150
YIDYVGSWGP AIIGHADDEV LAALAETMKK GTSFGAPCLL ENVLAEMVIS
160 170 180 190 200
AVPSIEMVRF VNSGTEACMG VLRLARAFTN KEKFIKFEGC YHGHANAFLV
210 220 230 240 250
KAGSGVATLG LPDSPGVPKA ATSDTLTAPY NDLEAVEKLF AAHKGEISAV
260 270 280 290 300
ILEPVVGNSG FIPPTPEFIN GLRQLTKDNG VLLIFDEVMT GFRLAYGGAQ
310 320 330 340 350
EYFGITPDLT TLGKIIGGGL PVGAYGGRRD IMEMVAPAGP MYQAGTLSGN
360 370 380 390 400
PLAMTAGIHT LKRLKQAGTY EYLDKITKEL TNGILEAGKK TGHPMCGGYI
410 420 430 440 450
SGMFGFFFAE GPVYNFADSK KSDTEKFGRF FRGMLEEGVY FAPSQFEAGF
460 470
TSLAHTPEDI QLTIAAAERV LSRI
Length:474
Mass (Da):50,370
Last modified:November 1, 1995 - v1
Checksum:i0D5E1A84F2B7433E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03773 Genomic DNA Translation: AAA19117.1
AB005234 Genomic DNA Translation: BAB10450.1
CP002688 Genomic DNA Translation: AED97770.1
AY102109 mRNA Translation: AAM26679.1
AY139804 mRNA Translation: AAM98110.1
RefSeqiNP_201162.1, NM_125752.4
UniGeneiAt.27758

Genome annotation databases

EnsemblPlantsiAT5G63570.1; AT5G63570.1; AT5G63570
GeneIDi836476
GrameneiAT5G63570.1; AT5G63570.1; AT5G63570
KEGGiath:AT5G63570

Similar proteinsi

Entry informationi

Entry nameiGSA1_ARATH
AccessioniPrimary (citable) accession number: P42799
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health