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Protein

Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic

Gene

GSA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transaminase converting glutamate 1-semialdehyde (GSA) to 5-aminolevulinate (ALA). Involved in the biosynthesis of tetrapyrroles.1 Publication

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At2g27450), Glutamyl-tRNA reductase (AXX17_At1g52270), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT5G63570-MONOMER.
MetaCyc:AT5G63570-MONOMER.
UniPathwayiUPA00251; UER00317.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (EC:5.4.3.8)
Short name:
GSA 1
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 1
Short name:
GSA-AT 1
Gene namesi
Name:GSA1
Synonyms:HEML1
Ordered Locus Names:At5g63570
ORF Names:MBK5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G63570.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastBy similarityAdd BLAST38
ChainiPRO_000000125539 – 474Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplasticAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP42799.
PRIDEiP42799.

Expressioni

Tissue specificityi

Present in all tissues tested.1 Publication

Inductioni

By light. In etiolated seedlings, initial expression is reduced but after further illumination, levels steadily increase.1 Publication

Gene expression databases

GenevisibleiP42799. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi21718. 2 interactors.
IntActiP42799. 1 interactor.
STRINGi3702.AT5G63570.1.

Structurei

Secondary structure

1474
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 60Combined sources10
Turni61 – 63Combined sources3
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Helixi71 – 74Combined sources4
Helixi76 – 78Combined sources3
Beta strandi85 – 90Combined sources6
Beta strandi92 – 95Combined sources4
Beta strandi100 – 105Combined sources6
Helixi106 – 108Combined sources3
Turni109 – 113Combined sources5
Helixi118 – 128Combined sources11
Helixi139 – 151Combined sources13
Beta strandi156 – 163Combined sources8
Helixi164 – 179Combined sources16
Beta strandi183 – 188Combined sources6
Helixi196 – 198Combined sources3
Beta strandi205 – 208Combined sources4
Beta strandi212 – 214Combined sources3
Helixi219 – 222Combined sources4
Beta strandi225 – 229Combined sources5
Helixi233 – 242Combined sources10
Turni244 – 246Combined sources3
Beta strandi247 – 252Combined sources6
Beta strandi254 – 256Combined sources3
Helixi266 – 278Combined sources13
Beta strandi282 – 286Combined sources5
Turni288 – 293Combined sources6
Helixi298 – 303Combined sources6
Beta strandi308 – 313Combined sources6
Helixi314 – 317Combined sources4
Beta strandi323 – 327Combined sources5
Helixi329 – 332Combined sources4
Turni336 – 338Combined sources3
Beta strandi339 – 341Combined sources3
Helixi351 – 364Combined sources14
Helixi369 – 390Combined sources22
Beta strandi396 – 400Combined sources5
Beta strandi403 – 411Combined sources9
Helixi416 – 419Combined sources4
Helixi424 – 436Combined sources13
Helixi457 – 471Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HDMX-ray1.25A/B41-474[»]
ProteinModelPortaliP42799.
SMRiP42799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IPFV. Eukaryota.
COG0001. LUCA.
HOGENOMiHOG000020210.
InParanoidiP42799.
KOiK01845.
OMAiMIVGHCH.
OrthoDBiEOG093608WJ.
PhylomeDBiP42799.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3. 1 hit.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATLTGSGT ALGFSCSSKI SKRVSSSPAS NRCCIKMSVS VDEKKKSFSL
60 70 80 90 100
QKSEEAFNAA KNLMPGGVNS PVRAFKSVGG QPVLIDSVKG SKMWDIDGNE
110 120 130 140 150
YIDYVGSWGP AIIGHADDEV LAALAETMKK GTSFGAPCLL ENVLAEMVIS
160 170 180 190 200
AVPSIEMVRF VNSGTEACMG VLRLARAFTN KEKFIKFEGC YHGHANAFLV
210 220 230 240 250
KAGSGVATLG LPDSPGVPKA ATSDTLTAPY NDLEAVEKLF AAHKGEISAV
260 270 280 290 300
ILEPVVGNSG FIPPTPEFIN GLRQLTKDNG VLLIFDEVMT GFRLAYGGAQ
310 320 330 340 350
EYFGITPDLT TLGKIIGGGL PVGAYGGRRD IMEMVAPAGP MYQAGTLSGN
360 370 380 390 400
PLAMTAGIHT LKRLKQAGTY EYLDKITKEL TNGILEAGKK TGHPMCGGYI
410 420 430 440 450
SGMFGFFFAE GPVYNFADSK KSDTEKFGRF FRGMLEEGVY FAPSQFEAGF
460 470
TSLAHTPEDI QLTIAAAERV LSRI
Length:474
Mass (Da):50,370
Last modified:November 1, 1995 - v1
Checksum:i0D5E1A84F2B7433E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03773 Genomic DNA. Translation: AAA19117.1.
AB005234 Genomic DNA. Translation: BAB10450.1.
CP002688 Genomic DNA. Translation: AED97770.1.
AY102109 mRNA. Translation: AAM26679.1.
AY139804 mRNA. Translation: AAM98110.1.
RefSeqiNP_201162.1. NM_125752.4.
UniGeneiAt.27758.

Genome annotation databases

EnsemblPlantsiAT5G63570.1; AT5G63570.1; AT5G63570.
GeneIDi836476.
GrameneiAT5G63570.1; AT5G63570.1; AT5G63570.
KEGGiath:AT5G63570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03773 Genomic DNA. Translation: AAA19117.1.
AB005234 Genomic DNA. Translation: BAB10450.1.
CP002688 Genomic DNA. Translation: AED97770.1.
AY102109 mRNA. Translation: AAM26679.1.
AY139804 mRNA. Translation: AAM98110.1.
RefSeqiNP_201162.1. NM_125752.4.
UniGeneiAt.27758.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HDMX-ray1.25A/B41-474[»]
ProteinModelPortaliP42799.
SMRiP42799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21718. 2 interactors.
IntActiP42799. 1 interactor.
STRINGi3702.AT5G63570.1.

Proteomic databases

PaxDbiP42799.
PRIDEiP42799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G63570.1; AT5G63570.1; AT5G63570.
GeneIDi836476.
GrameneiAT5G63570.1; AT5G63570.1; AT5G63570.
KEGGiath:AT5G63570.

Organism-specific databases

TAIRiAT5G63570.

Phylogenomic databases

eggNOGiENOG410IPFV. Eukaryota.
COG0001. LUCA.
HOGENOMiHOG000020210.
InParanoidiP42799.
KOiK01845.
OMAiMIVGHCH.
OrthoDBiEOG093608WJ.
PhylomeDBiP42799.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.
UPA00668.
BioCyciARA:AT5G63570-MONOMER.
MetaCyc:AT5G63570-MONOMER.

Miscellaneous databases

PROiP42799.

Gene expression databases

GenevisibleiP42799. AT.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3. 1 hit.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSA1_ARATH
AccessioniPrimary (citable) accession number: P42799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.