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Protein

Pseudomonalisin

Gene

pcp

Organism
Pseudomonas sp. (strain 101) (Achromobacter parvulus T1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of the B chain of insulin at 13-Glu-|-Ala-14, 15-Leu-|-Tyr-16 and 25-Phe-|-Tyr-26 and angiotensin I at 4-Tyr-|-Ile-5. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-|-Phe(NO2)-Arg-Leu.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei295Charge relay system1 Publication1
Active sitei299Charge relay system1 Publication1
Active sitei502Charge relay system1 Publication1
Metal bindingi543Calcium1
Metal bindingi544Calcium; via carbonyl oxygen1
Metal bindingi559Calcium; via carbonyl oxygen1
Metal bindingi561Calcium; via carbonyl oxygen1
Metal bindingi563Calcium1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.100. 5085.

Protein family/group databases

MEROPSiS53.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudomonalisin (EC:3.4.21.100)
Alternative name(s):
Pepstatin-insensitive carboxyl proteinase
Pseudomonapepsin
Gene namesi
Name:pcp
OrganismiPseudomonas sp. (strain 101) (Achromobacter parvulus T1)
Taxonomic identifieri33067 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000027362? – 215Removed in mature form
Signal peptidei1 – ?Sequence analysis
ChainiPRO_0000027363216 – 585PseudomonalisinAdd BLAST370
PropeptideiPRO_0000027364586 – 587Removed in mature form2

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi352 ↔ 391

Post-translational modificationi

Autocatalytically processed.

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

Secondary structure

1587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi225 – 227Combined sources3
Helixi228 – 231Combined sources4
Beta strandi240 – 252Combined sources13
Helixi254 – 266Combined sources13
Beta strandi274 – 278Combined sources5
Helixi290 – 306Combined sources17
Beta strandi311 – 319Combined sources9
Beta strandi321 – 324Combined sources4
Helixi327 – 339Combined sources13
Beta strandi343 – 347Combined sources5
Helixi353 – 358Combined sources6
Helixi361 – 374Combined sources14
Beta strandi378 – 382Combined sources5
Turni390 – 395Combined sources6
Turni407 – 409Combined sources3
Beta strandi413 – 423Combined sources11
Beta strandi429 – 434Combined sources6
Beta strandi436 – 439Combined sources4
Beta strandi445 – 447Combined sources3
Beta strandi451 – 457Combined sources7
Helixi460 – 464Combined sources5
Beta strandi470 – 474Combined sources5
Beta strandi476 – 480Combined sources5
Helixi483 – 485Combined sources3
Beta strandi487 – 491Combined sources5
Beta strandi494 – 498Combined sources5
Helixi501 – 518Combined sources18
Turni519 – 521Combined sources3
Helixi527 – 536Combined sources10
Helixi538 – 540Combined sources3
Beta strandi548 – 551Combined sources4
Beta strandi560 – 563Combined sources4
Turni564 – 566Combined sources3
Helixi573 – 583Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GA1X-ray1.40A216-587[»]
1GA4X-ray1.40A216-587[»]
1GA6X-ray1.00A216-587[»]
1KDVX-ray1.10A216-585[»]
1KDYX-ray1.10A216-585[»]
1KDZX-ray1.40A216-585[»]
1KE1X-ray1.80A216-585[»]
1KE2X-ray2.00A216-585[»]
1NLUX-ray1.30A216-585[»]
ProteinModelPortaliP42790.
SMRiP42790.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 583Peptidase S53Add BLAST365

Sequence similaritiesi

Contains 1 peptidase S53 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK05998.

Family and domain databases

CDDicd04056. Peptidases_S53. 1 hit.
cd11377. Pro-peptidase_S53. 1 hit.
Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR009020. Propept_inh.
IPR015366. S53_propep.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSAAKQTV LCLNRYAVVA LPLAIASFAA FGASPASTLW APTDTKAFVT
60 70 80 90 100
PAQVEARSAA PLLELAAGET AHIVVSLKLR DEAQLKQLAQ AVNQPGNAQF
110 120 130 140 150
GKFLKRRQFL SQFAPTEAQV QAVVAHLRKN GFVNIHVVPN RLLISADGSA
160 170 180 190 200
GAVKAAFNTP LVRYQLNGKA GYANTAPAQV PQDLGEIVGS VLGLQNVTRA
210 220 230 240 250
HPMLKVGERS AAKTLAAGTA KGHNPTEFPT IYDASSAPTA ANTTVGIITI
260 270 280 290 300
GGVSQTLQDL QQFTSANGLA SVNTQTIQTG SSNGDYSDDQ QGQGEWDLDS
310 320 330 340 350
QSIVGSAGGA VQQLLFYMAD QSASGNTGLT QAFNQAVSDN VAKVINVSLG
360 370 380 390 400
WCEADANADG TLQAEDRIFA TAAAQGQTFS VSSGDEGVYE CNNRGYPDGS
410 420 430 440 450
TYSVSWPASS PNVIAVGGTT LYTTSAGAYS NETVWNEGLD SNGKLWATGG
460 470 480 490 500
GYSVYESKPS WQSVVSGTPG RRLLPDISFD AAQGTGALIY NYGQLQQIGG
510 520 530 540 550
TSLASPIFVG LWARLQSANS NSLGFPAASF YSAISSTPSL VHDVKSGNNG
560 570 580
YGGYGYNAGT GWDYPTGWGS LDIAKLSAYI RSNGFGH
Length:587
Mass (Da):61,073
Last modified:November 1, 1995 - v1
Checksum:iE193D8B2C225829A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37970 Genomic DNA. Translation: BAA07188.1.

Genome annotation databases

KEGGiag:BAA07188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37970 Genomic DNA. Translation: BAA07188.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GA1X-ray1.40A216-587[»]
1GA4X-ray1.40A216-587[»]
1GA6X-ray1.00A216-587[»]
1KDVX-ray1.10A216-585[»]
1KDYX-ray1.10A216-585[»]
1KDZX-ray1.40A216-585[»]
1KE1X-ray1.80A216-585[»]
1KE2X-ray2.00A216-585[»]
1NLUX-ray1.30A216-585[»]
ProteinModelPortaliP42790.
SMRiP42790.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS53.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA07188.

Phylogenomic databases

KOiK05998.

Enzyme and pathway databases

BRENDAi3.4.21.100. 5085.

Miscellaneous databases

EvolutionaryTraceiP42790.

Family and domain databases

CDDicd04056. Peptidases_S53. 1 hit.
cd11377. Pro-peptidase_S53. 1 hit.
Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR009020. Propept_inh.
IPR015366. S53_propep.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPICP_PSESR
AccessioniPrimary (citable) accession number: P42790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.