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Protein

Lysosomal Pro-X carboxypeptidase

Gene

PRCP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

Catalytic activityi

Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Charge relay system1 Publication1
Active sitei430Charge relay system1 Publication1
Active sitei455Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

BioCyciZFISH:HS06356-MONOMER.
BRENDAi3.4.16.2. 2681.
ReactomeiR-HSA-140837. Intrinsic Pathway of Fibrin Clot Formation.
R-HSA-6798695. Neutrophil degranulation.

Protein family/group databases

ESTHERihuman-PRCP. Prolylcarboxypeptidase.
MEROPSiS28.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal Pro-X carboxypeptidase (EC:3.4.16.2)
Alternative name(s):
Angiotensinase C
Lysosomal carboxypeptidase C
Proline carboxypeptidase
Prolylcarboxypeptidase
Short name:
PRCP
Gene namesi
Name:PRCP
Synonyms:PCP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9344. PRCP.

Subcellular locationi

GO - Cellular componenti

  • basal part of cell Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • lysosome Source: UniProtKB-SubCell
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5547.
OpenTargetsiENSG00000137509.
PharmGKBiPA33705.

Chemistry databases

ChEMBLiCHEMBL2335.
GuidetoPHARMACOLOGYi1584.

Polymorphism and mutation databases

BioMutaiPRCP.
DMDMi1172047.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002730822 – 45Add BLAST24
ChainiPRO_000002730946 – 496Lysosomal Pro-X carboxypeptidaseAdd BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Glycosylationi101N-linked (GlcNAc...)1 Publication1
Disulfide bondi215 ↔ 3721 Publication
Disulfide bondi233 ↔ 3101 Publication
Disulfide bondi264 ↔ 3431 Publication
Glycosylationi317N-linked (GlcNAc...)2 Publications1
Glycosylationi336N-linked (GlcNAc...)1 Publication1
Glycosylationi345N-linked (GlcNAc...)1 Publication1
Disulfide bondi364 ↔ 3941 Publication
Glycosylationi415N-linked (GlcNAc...)2 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP42785.
MaxQBiP42785.
PaxDbiP42785.
PeptideAtlasiP42785.
PRIDEiP42785.

PTM databases

iPTMnetiP42785.
PhosphoSitePlusiP42785.

Expressioni

Tissue specificityi

Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.

Gene expression databases

BgeeiENSG00000137509.
CleanExiHS_PRCP.
ExpressionAtlasiP42785. baseline and differential.
GenevisibleiP42785. HS.

Organism-specific databases

HPAiHPA017065.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GPR37L1O608832EBI-2803892,EBI-2927498

Protein-protein interaction databases

BioGridi111538. 27 interactors.
IntActiP42785. 8 interactors.
MINTiMINT-3015634.
STRINGi9606.ENSP00000377055.

Chemistry databases

BindingDBiP42785.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi49 – 57Combined sources9
Beta strandi67 – 75Combined sources9
Turni81 – 83Combined sources3
Beta strandi85 – 90Combined sources6
Helixi96 – 102Combined sources7
Helixi104 – 113Combined sources10
Beta strandi115 – 120Combined sources6
Helixi132 – 136Combined sources5
Turni139 – 141Combined sources3
Helixi147 – 164Combined sources18
Helixi168 – 170Combined sources3
Beta strandi173 – 178Combined sources6
Helixi180 – 191Combined sources12
Turni193 – 195Combined sources3
Beta strandi197 – 202Combined sources6
Helixi217 – 227Combined sources11
Helixi233 – 247Combined sources15
Helixi251 – 260Combined sources10
Beta strandi263 – 265Combined sources3
Helixi272 – 288Combined sources17
Beta strandi295 – 301Combined sources7
Helixi305 – 312Combined sources8
Helixi320 – 336Combined sources17
Beta strandi342 – 344Combined sources3
Helixi354 – 365Combined sources12
Beta strandi375 – 380Combined sources6
Helixi387 – 398Combined sources12
Helixi406 – 411Combined sources6
Beta strandi422 – 429Combined sources8
Helixi431 – 435Combined sources5
Beta strandi441 – 450Combined sources10
Helixi457 – 459Combined sources3
Helixi468 – 489Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2ZX-ray2.79B46-491[»]
ProteinModelPortaliP42785.
SMRiP42785.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42785.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 334SKS domainAdd BLAST141

Sequence similaritiesi

Belongs to the peptidase S28 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2183. Eukaryota.
ENOG410Y38A. LUCA.
GeneTreeiENSGT00530000063027.
HOGENOMiHOG000238311.
HOVERGENiHBG005526.
InParanoidiP42785.
KOiK01285.
OMAiLEVRHMK.
OrthoDBiEOG091G056F.
PhylomeDBiP42785.
TreeFamiTF314414.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR008758. Peptidase_S28.
[Graphical view]
PfamiPF05577. Peptidase_S28. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRRALLLLL LSFLAPWATI ALRPALRALG SLHLPTNPTS LPAVAKNYSV
60 70 80 90 100
LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC
110 120 130 140 150
NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA
160 170 180 190 200
LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL
210 220 230 240 250
AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT
260 270 280 290 300
GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP
310 320 330 340 350
LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA
360 370 380 390 400
TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV
410 420 430 440 450
RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI
460 470 480 490
SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH
Length:496
Mass (Da):55,800
Last modified:November 1, 1995 - v1
Checksum:iFC5DD570D3D3AB4F
GO
Isoform 2 (identifier: P42785-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-56: K → KALAAGQLHICIIQLNHYKTPL

Note: No experimental confirmation available.
Show »
Length:517
Mass (Da):58,100
Checksum:iA2490258A57E6F23
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104G → E in BAG52417 (PubMed:14702039).Curated1
Sequence conflicti248S → L in BAG52417 (PubMed:14702039).Curated1
Sequence conflicti304W → R in BAG52417 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020464112E → D.1 PublicationCorresponds to variant rs2298668dbSNPEnsembl.1
Natural variantiVAR_029329444T → S.Corresponds to variant rs2228312dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04579956K → KALAAGQLHICIIQLNHYKT PL in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13977 mRNA. Translation: AAA99891.1.
AK091786 mRNA. Translation: BAG52417.1.
AK312919 mRNA. Translation: BAG35764.1.
AB451270 mRNA. Translation: BAG70084.1.
AB451397 mRNA. Translation: BAG70211.1.
AP000893 Genomic DNA. No translation available.
AP001646 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75074.1.
CH471076 Genomic DNA. Translation: EAW75075.1.
BC001500 mRNA. Translation: AAH01500.1.
CCDSiCCDS41695.1. [P42785-2]
CCDS8262.1. [P42785-1]
PIRiA47352.
RefSeqiNP_001306143.1. NM_001319214.1.
NP_005031.1. NM_005040.3. [P42785-1]
NP_955450.2. NM_199418.3. [P42785-2]
UniGeneiHs.523936.
Hs.733148.

Genome annotation databases

EnsembliENST00000313010; ENSP00000317362; ENSG00000137509. [P42785-1]
ENST00000393399; ENSP00000377055; ENSG00000137509. [P42785-2]
GeneIDi5547.
KEGGihsa:5547.
UCSCiuc001ozr.4. human. [P42785-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13977 mRNA. Translation: AAA99891.1.
AK091786 mRNA. Translation: BAG52417.1.
AK312919 mRNA. Translation: BAG35764.1.
AB451270 mRNA. Translation: BAG70084.1.
AB451397 mRNA. Translation: BAG70211.1.
AP000893 Genomic DNA. No translation available.
AP001646 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75074.1.
CH471076 Genomic DNA. Translation: EAW75075.1.
BC001500 mRNA. Translation: AAH01500.1.
CCDSiCCDS41695.1. [P42785-2]
CCDS8262.1. [P42785-1]
PIRiA47352.
RefSeqiNP_001306143.1. NM_001319214.1.
NP_005031.1. NM_005040.3. [P42785-1]
NP_955450.2. NM_199418.3. [P42785-2]
UniGeneiHs.523936.
Hs.733148.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2ZX-ray2.79B46-491[»]
ProteinModelPortaliP42785.
SMRiP42785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111538. 27 interactors.
IntActiP42785. 8 interactors.
MINTiMINT-3015634.
STRINGi9606.ENSP00000377055.

Chemistry databases

BindingDBiP42785.
ChEMBLiCHEMBL2335.
GuidetoPHARMACOLOGYi1584.

Protein family/group databases

ESTHERihuman-PRCP. Prolylcarboxypeptidase.
MEROPSiS28.001.

PTM databases

iPTMnetiP42785.
PhosphoSitePlusiP42785.

Polymorphism and mutation databases

BioMutaiPRCP.
DMDMi1172047.

Proteomic databases

EPDiP42785.
MaxQBiP42785.
PaxDbiP42785.
PeptideAtlasiP42785.
PRIDEiP42785.

Protocols and materials databases

DNASUi5547.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313010; ENSP00000317362; ENSG00000137509. [P42785-1]
ENST00000393399; ENSP00000377055; ENSG00000137509. [P42785-2]
GeneIDi5547.
KEGGihsa:5547.
UCSCiuc001ozr.4. human. [P42785-1]

Organism-specific databases

CTDi5547.
DisGeNETi5547.
GeneCardsiPRCP.
HGNCiHGNC:9344. PRCP.
HPAiHPA017065.
MIMi176785. gene.
neXtProtiNX_P42785.
OpenTargetsiENSG00000137509.
PharmGKBiPA33705.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2183. Eukaryota.
ENOG410Y38A. LUCA.
GeneTreeiENSGT00530000063027.
HOGENOMiHOG000238311.
HOVERGENiHBG005526.
InParanoidiP42785.
KOiK01285.
OMAiLEVRHMK.
OrthoDBiEOG091G056F.
PhylomeDBiP42785.
TreeFamiTF314414.

Enzyme and pathway databases

BioCyciZFISH:HS06356-MONOMER.
BRENDAi3.4.16.2. 2681.
ReactomeiR-HSA-140837. Intrinsic Pathway of Fibrin Clot Formation.
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiPRCP. human.
EvolutionaryTraceiP42785.
GeneWikiiPRCP.
GenomeRNAii5547.
PROiP42785.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137509.
CleanExiHS_PRCP.
ExpressionAtlasiP42785. baseline and differential.
GenevisibleiP42785. HS.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR008758. Peptidase_S28.
[Graphical view]
PfamiPF05577. Peptidase_S28. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCP_HUMAN
AccessioniPrimary (citable) accession number: P42785
Secondary accession number(s): A8MU24
, B2R7B7, B3KRK5, B5BU34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.