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Protein

Immunoglobulin A1 protease autotransporter

Gene

iga

Organism
Haemophilus influenzae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.

Catalytic activityi

Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei288 – 2881Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin A1 protease autotransporter (EC:3.4.21.72)
Cleaved into the following 2 chains:
Immunoglobulin A1 protease
Short name:
IGA1 protease
Alternative name(s):
Helper peptide
Gene namesi
Name:iga
OrganismiHaemophilus influenzae
Taxonomic identifieri727 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Subcellular locationi

Immunoglobulin A1 protease translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi288 – 2881S → T: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 15411516Immunoglobulin A1 protease autotransporterPRO_0000387599Add
BLAST
Chaini26 – 1008983Immunoglobulin A1 proteasePRO_0000026960Add
BLAST
Chaini1009 – 1541533Immunoglobulin A1 protease translocatorSequence analysisPRO_0000026961Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP42782.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 332307Peptidase S6PROSITE-ProRule annotationAdd
BLAST
Domaini1289 – 1541253AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGKFSVG
60 70 80 90 100
ATNVLVKDKN NKDLGTALPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH
110 120 130 140 150
VSNGVSELHF GNLNGNMNNG NAKAHRDVSS EENRYFSVEK NEYPTKLNGK
160 170 180 190 200
TVTTEDQTQK RREDYYMPRL DKFVTEVAPI EASTASSDAG TYNDQNKYPA
210 220 230 240 250
FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT YGIAGTPYKV
260 270 280 290 300
NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG
310 320 330 340 350
KWLFLGSYDF WAGYNKKSWQ EWNIYKSQFT KDVLNKDSAG SLIGSKTDYS
360 370 380 390 400
WSSNGKTSTI TGGEKSLNVD LADGKDKPNH GKSVTFEGSG TLTLNNNIDQ
410 420 430 440 450
GAGGLFFEGD YEVKGTSDNT TWKGAGVSVA EGKTVTWKVH NPQYDRLAKI
460 470 480 490 500
GKGTLIVEGT GDNKGSLKVG DGTVILKQQT NGSGQHAFAS VGIVSGRSTL
510 520 530 540 550
VLNDDKQVDP NSIYFGFRGG RLDLNGNSLT FDHIRNIDDG ARLVNHNMTN
560 570 580 590 600
ASNITITGES LITDPNTITP YNIDAPDEDN PYAFRRIKDG GQLYLNLENY
610 620 630 640 650
TYYALRKGAS TRSELPKNSG ESNENWLYMG KTSDEAKRNV MNHINNERMN
660 670 680 690 700
GFNGYFGEEE GKNNGNLNVT FKGKSEQNRF LLTGGTNLNG DLTVEKGTLF
710 720 730 740 750
LSGRPTPHAR DIAGISSTKK DPHFAENNEV VVEDDWINRN FKATTMNVTG
760 770 780 790 800
NASLYSGRNV ANITSNITAS NKAQVHIGYK TGDTVCVRSD YTGYVTCTTD
810 820 830 840 850
KLSDKALNSF NPTNLRGNVN LTESANFVLG KANLFGTIQS RGNSQVRLTE
860 870 880 890 900
NSHWHLTGNS DVHQLDLANG HIHLNSADNS NNVTKYNTLT VNSLSGNGSF
910 920 930 940 950
YYLTDLSNKQ GDKVVVTKSA TGNFTLQVAD KTGEPNHNEL TLFDASKAQR
960 970 980 990 1000
DHLNVSLVGN TVDLGAWKYK LRNVNGRYDL YNPEVEKRNQ TVDTTNITTP
1010 1020 1030 1040 1050
NNIQADVPSV PSNNEEIARV DEAPVPPPAP ATPSETTETV AENSKQESKT
1060 1070 1080 1090 1100
VEKNEQDATE TTAQNREVAK EAKSNVKANT QTNEVAQSGS ETKETQTTET
1110 1120 1130 1140 1150
KETATVEKEE KAKVETEKTQ EVPKVTSQVS PKQEQSETVQ PQAEPAREND
1160 1170 1180 1190 1200
PTVNIKEPQS QTNTTADTEQ PAKETSSNVE QPVTESTTVN TGNSVVENPE
1210 1220 1230 1240 1250
NTTPATTQPT VNSESSNKPK NRHRRSVRSV PHNVEPATTS SNDRSTVALC
1260 1270 1280 1290 1300
DLTSTNTNAV LSDARAKAQF VALNVGKAVS QHISQLEMNN EGQYNVWVSN
1310 1320 1330 1340 1350
TSMNKNYSSS QYRRFSSKST QTQLGWDQTI SNNVQLGGVF TYVRNSNNFD
1360 1370 1380 1390 1400
KATSKNTLAQ VNFYSKYYAD NHWYLGIDLG YGKFQSKLQT NHNAKFARHT
1410 1420 1430 1440 1450
AQFGLTAGKA FNLGNFGITP IVGVRYSYLS NADFALDQAR IKVNPISVKT
1460 1470 1480 1490 1500
AFAQVDLSYT YHLGEFSVTP ILSARYDANQ GSGKINVNGY DFAYNVENQQ
1510 1520 1530 1540
QYNAGLKLKY HNVKLSLIGG LTKAKQAEKQ KTAELKLSFS F
Length:1,541
Mass (Da):169,369
Last modified:November 1, 1995 - v1
Checksum:iCE7257CB3196C600
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64357 Genomic DNA. Translation: CAA45708.1.
M87492 Genomic DNA. Translation: AAA24969.1.
PIRiA37023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64357 Genomic DNA. Translation: CAA45708.1.
M87492 Genomic DNA. Translation: AAA24969.1.
PIRiA37023.

3D structure databases

ProteinModelPortaliP42782.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIGA1_HAEIF
AccessioniPrimary (citable) accession number: P42782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 16, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.