Reviewed, UniProtKB/Swiss-Prot P42782 (IGA1_HAEIN)

Names and origin

Protein names

Recommended name:
    Immunoglobulin A1 protease
      Short name=IGA1 protease
    EC=3.4.21.72

Gene names

Name:

iga

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.
Catalytic activity	Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.
Subcellular location	Secreted.
Domain	The signal peptide guides the precursor to the periplasmic space, and the C-terminal helper domain associates with the outer membrane to form a pore for excretion of the protease domain. The helper domain is then released by autoproteolysis By similarity.
Sequence similarities	Contains 1 autotransporter (TC 1.B.12) domain. [View classification] Contains 1 peptidase S6 domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1008	983	Immunoglobulin A1 protease		PRO_0000026960
	Propeptide	1009 – 1541	533	Helper peptide Potential		PRO_0000026961
Regions
	Domain	1289 – 1541	253	Autotransporter
Sites
	Active site	288	1	Probable
Experimental info
	Mutagenesis	288	1	S → T: Loss of activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P42782-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: CE7257CB3196C600	FASTA	1,541	169,369
	10 20 30 40 50 60 MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGKFSVG ATNVLVKDKN 70 80 90 100 110 120 NKDLGTALPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH VSNGVSELHF GNLNGNMNNG 130 140 150 160 170 180 NAKAHRDVSS EENRYFSVEK NEYPTKLNGK TVTTEDQTQK RREDYYMPRL DKFVTEVAPI 190 200 210 220 230 240 EASTASSDAG TYNDQNKYPA FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT 250 260 270 280 290 300 YGIAGTPYKV NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG 310 320 330 340 350 360 KWLFLGSYDF WAGYNKKSWQ EWNIYKSQFT KDVLNKDSAG SLIGSKTDYS WSSNGKTSTI 370 380 390 400 410 420 TGGEKSLNVD LADGKDKPNH GKSVTFEGSG TLTLNNNIDQ GAGGLFFEGD YEVKGTSDNT 430 440 450 460 470 480 TWKGAGVSVA EGKTVTWKVH NPQYDRLAKI GKGTLIVEGT GDNKGSLKVG DGTVILKQQT 490 500 510 520 530 540 NGSGQHAFAS VGIVSGRSTL VLNDDKQVDP NSIYFGFRGG RLDLNGNSLT FDHIRNIDDG 550 560 570 580 590 600 ARLVNHNMTN ASNITITGES LITDPNTITP YNIDAPDEDN PYAFRRIKDG GQLYLNLENY 610 620 630 640 650 660 TYYALRKGAS TRSELPKNSG ESNENWLYMG KTSDEAKRNV MNHINNERMN GFNGYFGEEE 670 680 690 700 710 720 GKNNGNLNVT FKGKSEQNRF LLTGGTNLNG DLTVEKGTLF LSGRPTPHAR DIAGISSTKK 730 740 750 760 770 780 DPHFAENNEV VVEDDWINRN FKATTMNVTG NASLYSGRNV ANITSNITAS NKAQVHIGYK 790 800 810 820 830 840 TGDTVCVRSD YTGYVTCTTD KLSDKALNSF NPTNLRGNVN LTESANFVLG KANLFGTIQS 850 860 870 880 890 900 RGNSQVRLTE NSHWHLTGNS DVHQLDLANG HIHLNSADNS NNVTKYNTLT VNSLSGNGSF 910 920 930 940 950 960 YYLTDLSNKQ GDKVVVTKSA TGNFTLQVAD KTGEPNHNEL TLFDASKAQR DHLNVSLVGN 970 980 990 1000 1010 1020 TVDLGAWKYK LRNVNGRYDL YNPEVEKRNQ TVDTTNITTP NNIQADVPSV PSNNEEIARV 1030 1040 1050 1060 1070 1080 DEAPVPPPAP ATPSETTETV AENSKQESKT VEKNEQDATE TTAQNREVAK EAKSNVKANT 1090 1100 1110 1120 1130 1140 QTNEVAQSGS ETKETQTTET KETATVEKEE KAKVETEKTQ EVPKVTSQVS PKQEQSETVQ 1150 1160 1170 1180 1190 1200 PQAEPAREND PTVNIKEPQS QTNTTADTEQ PAKETSSNVE QPVTESTTVN TGNSVVENPE 1210 1220 1230 1240 1250 1260 NTTPATTQPT VNSESSNKPK NRHRRSVRSV PHNVEPATTS SNDRSTVALC DLTSTNTNAV 1270 1280 1290 1300 1310 1320 LSDARAKAQF VALNVGKAVS QHISQLEMNN EGQYNVWVSN TSMNKNYSSS QYRRFSSKST 1330 1340 1350 1360 1370 1380 QTQLGWDQTI SNNVQLGGVF TYVRNSNNFD KATSKNTLAQ VNFYSKYYAD NHWYLGIDLG 1390 1400 1410 1420 1430 1440 YGKFQSKLQT NHNAKFARHT AQFGLTAGKA FNLGNFGITP IVGVRYSYLS NADFALDQAR 1450 1460 1470 1480 1490 1500 IKVNPISVKT AFAQVDLSYT YHLGEFSVTP ILSARYDANQ GSGKINVNGY DFAYNVENQQ 1510 1520 1530 1540 QYNAGLKLKY HNVKLSLIGG LTKAKQAEKQ KTAELKLSFS F

References

[1]	"Cloning and sequencing of the immunoglobulin A1 protease gene (iga) of Haemophilus influenzae serotype b." Poulsen K., Brandt J., Hjorth J.P., Thoegersen H.C., Kilian M. Infect. Immun. 57:3097-3105(1989) [PubMed: 2506130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HK368 / Serotype B.
[2]	"A comparative genetic study of serologically distinct Haemophilus influenzae type 1 immunoglobulin A1 proteases." Poulsen K., Reinholdt J., Kilian M. J. Bacteriol. 174:2913-2921(1992) [PubMed: 1373717] [Abstract] Cited for: MUTAGENESIS OF SER-288. Strain: HK368 / Serotype B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64357 Genomic DNA. Translation: CAA45708.1. M87492 Genomic DNA. Translation: AAA24969.1.
PIR	A37023.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.72. 109.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR012332. P22_tailspike. IPR000710. Peptidase_S6. IPR004899. Pertactin. [Graphical view]
Gene3D	G3DSA:2.160.20.20. P22_tailspike. 1 hit.
Pfam	PF03797. Autotransporter. 1 hit. PF02395. Peptidase_S6. 1 hit. PF03212. Pertactin. 1 hit. [Graphical view]
PRINTS	PR00921. IGASERPTASE.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGA1_HAEIN

Accession

Primary (citable) accession number: P42782

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)