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Protein

Glutathione reductase, chloroplastic

Gene

EMB2360

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the chloroplast.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei291NADPBy similarity1
Binding sitei297NADPBy similarity1
Active sitei529Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 135FADBy similarity10

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • electron transfer activity Source: InterPro
  • flavin adenine dinucleotide binding Source: InterPro
  • glutathione-disulfide reductase activity Source: UniProtKB-EC
  • NADP binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species
R-ATH-499943 Interconversion of nucleotide di- and triphosphates
R-ATH-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, chloroplastic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Alternative name(s):
Protein EMBRYO DEFECTIVE 2360
Gene namesi
Name:EMB2360
Ordered Locus Names:At3g54660
ORF Names:T5N23_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54660
TAIRilocus:2102410 AT3G54660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 74ChloroplastSequence analysisAdd BLAST74
ChainiPRO_000003028075 – 565Glutathione reductase, chloroplasticAdd BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi135 ↔ 140Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP42770
PRIDEiP42770

PTM databases

SwissPalmiP42770

Expressioni

Gene expression databases

ExpressionAtlasiP42770 baseline and differential
GenevisibleiP42770 AT

Interactioni

Protein-protein interaction databases

BioGridi9947, 5 interactors
IntActiP42770, 1 interactor
STRINGi3702.AT3G54660.1

Structurei

3D structure databases

ProteinModelPortaliP42770
SMRiP42770
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
HOGENOMiHOG000276712
InParanoidiP42770
KOiK00383
OMAiKCAIIEA
OrthoDBiEOG093608FG
PhylomeDBiP42770

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR006324 Glut-diS_reduct
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01424 gluta_reduc_2, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTPKLTST ISSSSPSLQF LCKKLPIAIH LPSSSSSSFL SLPKTLTSLY
60 70 80 90 100
SLRPRIALLS NHRYYHSRRF SVCASTDNGA ESDRHYDFDL FTIGAGSGGV
110 120 130 140 150
RASRFATSFG ASAAVCELPF STISSDTAGG VGGTCVLRGC VPKKLLVYAS
160 170 180 190 200
KYSHEFEDSH GFGWKYETEP SHDWTTLIAN KNAELQRLTG IYKNILSKAN
210 220 230 240 250
VKLIEGRGKV IDPHTVDVDG KIYTTRNILI AVGGRPFIPD IPGKEFAIDS
260 270 280 290 300
DAALDLPSKP KKIAIVGGGY IALEFAGIFN GLNCEVHVFI RQKKVLRGFD
310 320 330 340 350
EDVRDFVGEQ MSLRGIEFHT EESPEAIIKA GDGSFSLKTS KGTVEGFSHV
360 370 380 390 400
MFATGRKPNT KNLGLENVGV KMAKNGAIEV DEYSQTSVPS IWAVGDVTDR
410 420 430 440 450
INLTPVALME GGALAKTLFQ NEPTKPDYRA VPCAVFSQPP IGTVGLTEEQ
460 470 480 490 500
AIEQYGDVDV YTSNFRPLKA TLSGLPDRVF MKLIVCANTN KVLGVHMCGE
510 520 530 540 550
DSPEIIQGFG VAVKAGLTKA DFDATVGVHP TAAEEFVTMR APTRKFRKDS
560
SEGKASPEAK TAAGV
Length:565
Mass (Da):60,852
Last modified:November 1, 1995 - v1
Checksum:i29C2CD0E68ED4E4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14049 mRNA Translation: BAA03137.1
D89620 Genomic DNA Translation: BAA19653.1
AL138650 Genomic DNA Translation: CAB77586.1
CP002686 Genomic DNA Translation: AEE79262.1
PIRiT47625
RefSeqiNP_191026.1, NM_115323.4
UniGeneiAt.21776

Genome annotation databases

EnsemblPlantsiAT3G54660.1; AT3G54660.1; AT3G54660
GeneIDi824631
GrameneiAT3G54660.1; AT3G54660.1; AT3G54660
KEGGiath:AT3G54660

Similar proteinsi

Entry informationi

Entry nameiGSHRP_ARATH
AccessioniPrimary (citable) accession number: P42770
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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