P42769 (GSTF1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase PM239X14 EC=2.5.1.18 Alternative name(s): GST class-phi
Organism	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier	3702 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically catalyzes the conjugation of synthetic 1-chloro-2,4-ditrobenzene to GSH. Also functions as a glutathione peroxidase, converting linoleate oxidation products into their corresponding hydroxyacids. This enzyme may thus serve to protect the cell from oxygen toxicity as well as from exogenous toxins such as herbicides. Ref.1
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Tissue specificity	Expressed in vegetative rosettes.
Developmental stage	Expressed up until the first flowering buds, after which, levels dramatically decrease.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	This protein probably does not originate from A.thaliana, rather it resemble fungal GTSs.

Ontologies

Keywords
Biological process	Detoxification Stress response
Cellular component	Cytoplasm
Molecular function	Oxidoreductase Peroxidase Transferase
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Glutathione S-transferase PM239X14		PRO_0000185849
Regions
	Domain	2 – 85	84	GST N-terminal
	Domain	93 – 218	126	GST C-terminal
	Region	12 – 13	2	Glutathione binding By similarity
	Region	41 – 42	2	Glutathione binding By similarity
	Region	55 – 56	2	Glutathione binding By similarity
	Region	69 – 70	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42769 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C7E6B6EC5320C33A	FASTA	218	24,364
	10 20 30 40 50 60 MVTVKLYGMA YSTCTKRVYT TAKEIGVDVK IVPVDLMKGE HKEPAYLDNY HPFGVIPVLE 70 80 90 100 110 120 DEDGTKIYES RAISRYLVAK YGKGSSLLPS PSDPKAYGLF EQAASVEYSS FDPPASSLAY 130 140 150 160 170 180 ERVFAGMRGL KTNEELAKKY VDTLNAKMDG YERILSKQKY LAGNDFTLAD LFHLPYGAMV 190 200 210 AQLEPTVLDS KPHVKAWWAA SLRVIPGRLL RNSSKEFM

References

[1]	"A glutathione S-transferase with glutathione-peroxidase activity from Arabidopsis thaliana. Molecular cloning and functional characterization." Bartling D., Radzio R., Steiner U., Weiler E.W. Eur. J. Biochem. 216:579-586(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Landsberg erecta. Tissue: Leaf.
[2]	Erratum Bartling D., Radzio R., Steiner U., Weiler E.W. Eur. J. Biochem. 218:1096-1096(1993)
[3]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68304 mRNA. Translation: CAA48376.1.
IPI	IPI00530259.
PIR	S36835.
3D structure databases
ProteinModelPortal	P42769.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Gene expression databases
Genevestigator	P42769.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF1_ARATH

Accession

Primary (citable) accession number: P42769

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program