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P42765

- THIM_HUMAN

UniProt

P42765 - THIM_HUMAN

Protein

3-ketoacyl-CoA thiolase, mitochondrial

Gene

ACAA2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Abolishes BNIP3-mediated apoptosis and mitochondrial damage.1 Publication

    Catalytic activityi

    Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Acyl-thioester intermediateBy similarity
    Active sitei352 – 3521Proton acceptorPROSITE-ProRule annotation
    Active sitei382 – 3821Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. acetyl-CoA C-acyltransferase activity Source: UniProtKB
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to hypoxia Source: BHF-UCL
    2. cholesterol biosynthetic process Source: UniProtKB
    3. fatty acid metabolic process Source: UniProtKB-UniPathway
    4. negative regulation of mitochondrial membrane permeability involved in apoptotic process Source: BHF-UCL
    5. negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:HS09539-MONOMER.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-ketoacyl-CoA thiolase, mitochondrial (EC:2.3.1.16)
    Alternative name(s):
    Acetyl-CoA acyltransferase
    Beta-ketothiolase
    Mitochondrial 3-oxoacyl-CoA thiolase
    T1
    Gene namesi
    Name:ACAA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:83. ACAA2.

    Subcellular locationi

    Mitochondrion 1 Publication
    Note: Colocalizes with BNIP3 in the mitochondria.

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. mitochondrial inner membrane Source: Ensembl
    3. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24420.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3973973-ketoacyl-CoA thiolase, mitochondrialPRO_0000223299Add
    BLAST
    Transit peptidei1 – 1616Mitochondrion; not cleavedAdd
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251N6-acetyllysine; alternateBy similarity
    Modified residuei25 – 251N6-succinyllysine; alternateBy similarity
    Modified residuei45 – 451N6-succinyllysineBy similarity
    Modified residuei119 – 1191Phosphothreonine1 Publication
    Modified residuei121 – 1211Phosphoserine1 Publication
    Modified residuei127 – 1271Phosphotyrosine1 Publication
    Modified residuei137 – 1371N6-acetyllysine; alternateBy similarity
    Modified residuei137 – 1371N6-succinyllysine; alternateBy similarity
    Modified residuei143 – 1431N6-acetyllysine; alternateBy similarity
    Modified residuei143 – 1431N6-succinyllysine; alternateBy similarity
    Modified residuei171 – 1711N6-acetyllysine; alternateBy similarity
    Modified residuei171 – 1711N6-succinyllysine; alternateBy similarity
    Modified residuei191 – 1911N6-acetyllysine; alternateBy similarity
    Modified residuei191 – 1911N6-succinyllysine; alternateBy similarity
    Modified residuei209 – 2091N6-acetyllysine; alternateBy similarity
    Modified residuei209 – 2091N6-succinyllysine; alternateBy similarity
    Modified residuei211 – 2111N6-succinyllysineBy similarity
    Modified residuei212 – 2121N6-succinyllysineBy similarity
    Modified residuei214 – 2141N6-succinyllysineBy similarity
    Modified residuei234 – 2341N6-acetyllysine; alternateBy similarity
    Modified residuei234 – 2341N6-succinyllysine; alternateBy similarity
    Modified residuei240 – 2401N6-succinyllysineBy similarity
    Modified residuei241 – 2411N6-acetyllysineBy similarity
    Modified residuei269 – 2691N6-acetyllysineBy similarity
    Modified residuei270 – 2701N6-acetyllysineBy similarity
    Modified residuei305 – 3051N6-acetyllysine; alternateBy similarity
    Modified residuei305 – 3051N6-succinyllysine; alternateBy similarity
    Modified residuei312 – 3121N6-acetyllysine; alternateBy similarity
    Modified residuei312 – 3121N6-succinyllysine; alternateBy similarity
    Modified residuei340 – 3401N6-acetyllysineBy similarity
    Modified residuei375 – 3751N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP42765.
    PaxDbiP42765.
    PeptideAtlasiP42765.
    PRIDEiP42765.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00001539.
    UCD-2DPAGEP42765.

    PTM databases

    PhosphoSiteiP42765.

    Expressioni

    Gene expression databases

    ArrayExpressiP42765.
    BgeeiP42765.
    GenevestigatoriP42765.

    Organism-specific databases

    HPAiHPA042303.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with BNIP3.1 Publication

    Protein-protein interaction databases

    BioGridi115713. 21 interactions.
    IntActiP42765. 2 interactions.
    MINTiMINT-5005997.
    STRINGi9606.ENSP00000285093.

    Structurei

    3D structure databases

    ProteinModelPortaliP42765.
    SMRiP42765. Positions 4-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiolase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0183.
    HOGENOMiHOG000012238.
    HOVERGENiHBG003112.
    KOiK07508.
    OMAiQTLACAD.
    OrthoDBiEOG7JQBNG.
    PhylomeDBiP42765.
    TreeFamiTF105696.

    Family and domain databases

    Gene3Di3.40.47.10. 4 hits.
    InterProiIPR002155. Thiolase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    IPR020615. Thiolase_acyl_enz_int_AS.
    IPR020610. Thiolase_AS.
    IPR020617. Thiolase_C.
    IPR020613. Thiolase_CS.
    IPR020616. Thiolase_N.
    [Graphical view]
    PfamiPF02803. Thiolase_C. 1 hit.
    PF00108. Thiolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
    PROSITEiPS00098. THIOLASE_1. 1 hit.
    PS00737. THIOLASE_2. 1 hit.
    PS00099. THIOLASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42765-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLRGVFVV AAKRTPFGAY GGLLKDFTAT DLSEFAAKAA LSAGKVSPET    50
    VDSVIMGNVL QSSSDAIYLA RHVGLRVGIP KETPALTINR LCGSGFQSIV 100
    NGCQEICVKE AEVVLCGGTE SMSQAPYCVR NVRFGTKLGS DIKLEDSLWV 150
    SLTDQHVQLP MAMTAENLAV KHKISREECD KYALQSQQRW KAANDAGYFN 200
    DEMAPIEVKT KKGKQTMQVD EHARPQTTLE QLQKLPPVFK KDGTVTAGNA 250
    SGVADGAGAV IIASEDAVKK HNFTPLARIV GYFVSGCDPS IMGIGPVPAI 300
    SGALKKAGLS LKDMDLVEVN EAFAPQYLAV ERSLDLDISK TNVNGGAIAL 350
    GHPLGGSGSR ITAHLVHELR RRGGKYAVGS ACIGGGQGIA VIIQSTA 397
    Length:397
    Mass (Da):41,924
    Last modified:January 4, 2005 - v2
    Checksum:i380CC8262ACF6D01
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21A → R in BAA03800. (PubMed:8241273)Curated
    Sequence conflicti169 – 1691A → T in BAA03800. (PubMed:8241273)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti217 – 2171M → V.
    Corresponds to variant rs11549285 [ dbSNP | Ensembl ].
    VAR_052577

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16294 mRNA. Translation: BAA03800.1.
    BC001918 mRNA. Translation: AAH01918.1.
    CCDSiCCDS11939.1.
    PIRiS43440.
    RefSeqiNP_006102.2. NM_006111.2.
    UniGeneiHs.200136.

    Genome annotation databases

    EnsembliENST00000285093; ENSP00000285093; ENSG00000167315.
    GeneIDi10449.
    KEGGihsa:10449.
    UCSCiuc002ldw.4. human.

    Polymorphism databases

    DMDMi57015371.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16294 mRNA. Translation: BAA03800.1 .
    BC001918 mRNA. Translation: AAH01918.1 .
    CCDSi CCDS11939.1.
    PIRi S43440.
    RefSeqi NP_006102.2. NM_006111.2.
    UniGenei Hs.200136.

    3D structure databases

    ProteinModelPortali P42765.
    SMRi P42765. Positions 4-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115713. 21 interactions.
    IntActi P42765. 2 interactions.
    MINTi MINT-5005997.
    STRINGi 9606.ENSP00000285093.

    PTM databases

    PhosphoSitei P42765.

    Polymorphism databases

    DMDMi 57015371.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00001539.
    UCD-2DPAGE P42765.

    Proteomic databases

    MaxQBi P42765.
    PaxDbi P42765.
    PeptideAtlasi P42765.
    PRIDEi P42765.

    Protocols and materials databases

    DNASUi 10449.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000285093 ; ENSP00000285093 ; ENSG00000167315 .
    GeneIDi 10449.
    KEGGi hsa:10449.
    UCSCi uc002ldw.4. human.

    Organism-specific databases

    CTDi 10449.
    GeneCardsi GC18M047309.
    H-InvDB HIX0014445.
    HGNCi HGNC:83. ACAA2.
    HPAi HPA042303.
    MIMi 604770. gene.
    neXtProti NX_P42765.
    PharmGKBi PA24420.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0183.
    HOGENOMi HOG000012238.
    HOVERGENi HBG003112.
    KOi K07508.
    OMAi QTLACAD.
    OrthoDBi EOG7JQBNG.
    PhylomeDBi P42765.
    TreeFami TF105696.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci MetaCyc:HS09539-MONOMER.

    Miscellaneous databases

    ChiTaRSi ACAA2. human.
    GeneWikii ACAA2.
    GenomeRNAii 10449.
    NextBioi 39605.
    PROi P42765.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P42765.
    Bgeei P42765.
    Genevestigatori P42765.

    Family and domain databases

    Gene3Di 3.40.47.10. 4 hits.
    InterProi IPR002155. Thiolase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    IPR020615. Thiolase_acyl_enz_int_AS.
    IPR020610. Thiolase_AS.
    IPR020617. Thiolase_C.
    IPR020613. Thiolase_CS.
    IPR020616. Thiolase_N.
    [Graphical view ]
    Pfami PF02803. Thiolase_C. 1 hit.
    PF00108. Thiolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    TIGRFAMsi TIGR01930. AcCoA-C-Actrans. 1 hit.
    PROSITEi PS00098. THIOLASE_1. 1 hit.
    PS00737. THIOLASE_2. 1 hit.
    PS00099. THIOLASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a full length cDNA encoding human mitochondrial 3-oxoacyl-CoA thiolase."
      Abe H., Ohtake A., Yamamoto S., Satoh Y., Takayanagi M., Amaya Y., Takiguchi M., Sakuraba H., Suzuki Y., Mori M., Niimi H.
      Biochim. Biophys. Acta 1216:304-306(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    3. "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes."
      Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.
      Biochem. J. 383:237-248(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 15-25; 46-71 AND 341-360.
      Tissue: Adipocyte.
    4. "Acetyl-Coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines."
      Cao W., Liu N., Tang S., Bao L., Shen L., Yuan H., Zhao X., Lu H.
      Biochim. Biophys. Acta 1780:873-880(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BNIP3.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-119; SER-121 AND TYR-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTHIM_HUMAN
    AccessioniPrimary (citable) accession number: P42765
    Secondary accession number(s): Q9BUT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3