Glutathione S-transferase F10 - Arabidopsis thaliana (Mouse-ear cress)

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P42761 (GSTFA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F10
Short name=AtGSTF10
EC=2.5.1.18

Alternative name(s):
AtGSTF4
GST class-phi member 10
Protein EARLY RESPONSE TO DEHYDRATION 13

Gene names

Name:	GSTF10
Synonyms:	ERD13, GSTF4
Ordered Locus Names:	At2g30870
ORF Names:	F7F1.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.7
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Interacts with BAK1. Ref.8
Subcellular location	Cytoplasm › cytosol Probable.
Tissue specificity	Expressed in roots, stems, floral buds, mature flowers and leaves. Ref.8
Induction	By dehydration stress, wounding, H₂O₂ and jasmonate, but not by growth regulators. Ref.6 Ref.8
Disruption phenotype	No visible phenotype, maybe due to the possible redundancy with GSTF9. Ref.8
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification Stress response
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW response to water deprivation Inferred from expression pattern Ref.1. Source: TAIR toxin catabolic process Traceable author statement Ref.7. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR cell wall Inferred from direct assay PubMed 16287169. Source: TAIR chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	copper ion binding Inferred from direct assay PubMed 16526091 PubMed 20018591. Source: TAIR glutathione binding Inferred from direct assay PubMed 15159623. Source: TAIR glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 215

214

Glutathione S-transferase F10

PRO_0000185847

Regions

Domain

2 – 81

GST N-terminal

Domain

88 – 215

128

GST C-terminal

Region

39 – 40

Glutathione binding By similarity

Region

52 – 53

Glutathione binding By similarity

Region

65 – 66

Glutathione binding By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P42761 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9A53C0AD19580C79
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FASTA

215

24,230

        10         20         30         40         50         60 
MVLTIYAPLF ASSKRAVVTL VEKGVSFETV NVDLMKGEQR QPEYLAIQPF GKIPVLVDGD 

        70         80         90        100        110        120 
YKIFESRAIM RYIAEKYRSQ GPDLLGKTIE ERGQVEQWLD VEATSYHPPL LALTLNIVFA 

       130        140        150        160        170        180 
PLMGFPADEK VIKESEEKLA EVLDVYEAQL SKNEYLAGDF VSLADLAHLP FTEYLVGPIG 

       190        200        210 
KAHLIKDRKH VSAWWDKISS RAAWKEVSAK YSLPV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L." Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 335:189-192(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants." Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R. J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7. Strain: cv. Landsberg erecta.
[6]	"Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase." Bianchi M.W., Roux C., Vartanian N. Physiol. Plantarum 116:96-105(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[8]	"Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death." Ryu H.Y., Kim S.Y., Park H.M., You J.Y., Kim B.H., Lee J.S., Nam K.H. Biochem. Biophys. Res. Commun. 379:417-422(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION BY GROWTH REGULATORS, DISRUPTION PHENOTYPE, INTERACTION WITH BAK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17673 mRNA. Translation: BAA04554.1. AC004669 Genomic DNA. Translation: AAC20721.1. CP002685 Genomic DNA. Translation: AEC08450.1. AY128398 mRNA. Translation: AAM91601.1. BT000077 mRNA. Translation: AAN15396.1.
IPI	IPI00535478.
PIR	S39542.
RefSeq	NP_180644.1. NM_128639.3.
UniGene	At.58604. At.71298.
3D structure databases
ProteinModelPortal	P42761.
SMR	P42761. Positions 8-214.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42761. 1 interaction.
STRING	3702.AT2G30870.1-P.
2D gel databases
SWISS-2DPAGE	P42761.
Proteomic databases
PaxDb	P42761.
PRIDE	P42761.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30870.1; AT2G30870.1; AT2G30870.
GeneID	817637.
KEGG	ath:AT2G30870.
Organism-specific databases
TAIR	At2g30870.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
InParanoid	P42761.
KO	K00799.
OMA	AIRGWIS.
PhylomeDB	P42761.
ProtClustDB	PLN02395.
Gene expression databases
ArrayExpress	P42761.
Genevestigator	P42761.
GermOnline	AT2G30870. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTFA_ARATH

Accession

Primary (citable) accession number: P42761

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents