Reviewed, UniProtKB/Swiss-Prot P42761 (GSTF3_ARATH)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase ERD13
    EC=2.5.1.18
Alternative name(s):
    GST class-phi

Gene names

Name:	ERD13
Ordered Locus Names:	At2g30870
ORF Names:	F7F1.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. In plants, may have a detoxification role against certain herbicides.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm Probable.
Induction	By dehydration stress.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from expression pattern. Source: TAIR response to water deprivation Ref.1 Inferred from expression pattern. Source: TAIR toxin catabolic process Traceable author statement. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR cell wall Inferred from direct assay. Source: TAIR chloroplast Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	copper ion binding Inferred from direct assay. Source: TAIR glutathione binding Inferred from direct assay. Source: TAIR glutathione transferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BAK1	Q94F62	2	EBI-2265779,EBI-617138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 215	214	Glutathione S-transferase ERD13		PRO_0000185847
Regions
	Domain	2 – 81	80	GST N-terminal
	Domain	88 – 215	128	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	P42761-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9A53C0AD19580C79	FASTA	215	24,230
	10 20 30 40 50 60 MVLTIYAPLF ASSKRAVVTL VEKGVSFETV NVDLMKGEQR QPEYLAIQPF GKIPVLVDGD 70 80 90 100 110 120 YKIFESRAIM RYIAEKYRSQ GPDLLGKTIE ERGQVEQWLD VEATSYHPPL LALTLNIVFA 130 140 150 160 170 180 PLMGFPADEK VIKESEEKLA EVLDVYEAQL SKNEYLAGDF VSLADLAHLP FTEYLVGPIG 190 200 210 KAHLIKDRKH VSAWWDKISS RAAWKEVSAK YSLPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L." Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 335:189-192(1993) [PubMed: 8253194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants." Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R. J. Biol. Chem. 272:15841-15848(1997) [PubMed: 9188482] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D17673 mRNA. Translation: BAA04554.1. AC004669 Genomic DNA. Translation: AAC20721.1. AY128398 mRNA. Translation: AAM91601.1. BT000077 mRNA. Translation: AAN15396.1.
IPI	IPI00535478.
PIR	S39542.
RefSeq	NP_180644.1.
UniGene	At.58604 At.71298
3D structure databases
HSSP	HSSP built from PDB template 1AW9 based on UniProtKB Q9ZP62.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42761. 1 interaction.
2-D gel databases
SWISS-2DPAGE	P42761.
Proteomic databases
PRIDE	P42761.
Genome annotation databases
GeneID	817637.
GenomeReviews	Gene locus AT2G30870 in contig CT485783_GR.
KEGG	ath:AT2G30870.
NMPDR	fig|3702.1.peg.10141.
Organism-specific databases
TAIR	At2g30870.
Phylogenomic databases
OMA	P42761. SAPCQSI.
Enzyme and pathway databases
BRENDA	2.5.1.18. 302.
Gene expression databases
ArrayExpress	P42761.
GermOnline	AT2G30870. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF3_ARATH

Accession

Primary (citable) accession number: P42761

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)