Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase F10

Gene

GSTF10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • glutathione binding Source: TAIR
  • glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • response to toxic substance Source: UniProtKB-KW
  • response to water deprivation Source: TAIR
  • toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G30870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F10 (EC:2.5.1.18)
Short name:
AtGSTF10
Alternative name(s):
AtGSTF4
GST class-phi member 10
Protein EARLY RESPONSE TO DEHYDRATION 13
Gene namesi
Name:GSTF10
Synonyms:ERD13, GSTF4
Ordered Locus Names:At2g30870
ORF Names:F7F1.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30870.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, maybe due to the possible redundancy with GSTF9.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 215214Glutathione S-transferase F10PRO_0000185847Add
BLAST

Proteomic databases

PaxDbiP42761.
PRIDEiP42761.

2D gel databases

SWISS-2DPAGEP42761.

Expressioni

Tissue specificityi

Expressed in roots, stems, floral buds, mature flowers and leaves.1 Publication

Inductioni

By dehydration stress, wounding, H2O2 and jasmonate, but not by growth regulators.2 Publications

Gene expression databases

GenevestigatoriP42761.

Interactioni

Subunit structurei

Interacts with BAK1.1 Publication

Protein-protein interaction databases

IntActiP42761. 1 interaction.
MINTiMINT-8068911.
STRINGi3702.AT2G30870.1-P.

Structurei

3D structure databases

ProteinModelPortaliP42761.
SMRiP42761. Positions 8-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminalAdd
BLAST
Domaini88 – 215128GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 402Glutathione bindingBy similarity
Regioni52 – 532Glutathione bindingBy similarity
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125746.
InParanoidiP42761.
KOiK00799.
OMAiFTEYLVG.
PhylomeDBiP42761.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTIYAPLF ASSKRAVVTL VEKGVSFETV NVDLMKGEQR QPEYLAIQPF
60 70 80 90 100
GKIPVLVDGD YKIFESRAIM RYIAEKYRSQ GPDLLGKTIE ERGQVEQWLD
110 120 130 140 150
VEATSYHPPL LALTLNIVFA PLMGFPADEK VIKESEEKLA EVLDVYEAQL
160 170 180 190 200
SKNEYLAGDF VSLADLAHLP FTEYLVGPIG KAHLIKDRKH VSAWWDKISS
210
RAAWKEVSAK YSLPV
Length:215
Mass (Da):24,230
Last modified:January 23, 2007 - v3
Checksum:i9A53C0AD19580C79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17673 mRNA. Translation: BAA04554.1.
AC004669 Genomic DNA. Translation: AAC20721.1.
CP002685 Genomic DNA. Translation: AEC08450.1.
AY128398 mRNA. Translation: AAM91601.1.
BT000077 mRNA. Translation: AAN15396.1.
PIRiS39542.
RefSeqiNP_180644.1. NM_128639.3.
UniGeneiAt.58604.
At.71298.

Genome annotation databases

EnsemblPlantsiAT2G30870.1; AT2G30870.1; AT2G30870.
GeneIDi817637.
KEGGiath:AT2G30870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17673 mRNA. Translation: BAA04554.1.
AC004669 Genomic DNA. Translation: AAC20721.1.
CP002685 Genomic DNA. Translation: AEC08450.1.
AY128398 mRNA. Translation: AAM91601.1.
BT000077 mRNA. Translation: AAN15396.1.
PIRiS39542.
RefSeqiNP_180644.1. NM_128639.3.
UniGeneiAt.58604.
At.71298.

3D structure databases

ProteinModelPortaliP42761.
SMRiP42761. Positions 8-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42761. 1 interaction.
MINTiMINT-8068911.
STRINGi3702.AT2G30870.1-P.

2D gel databases

SWISS-2DPAGEP42761.

Proteomic databases

PaxDbiP42761.
PRIDEiP42761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30870.1; AT2G30870.1; AT2G30870.
GeneIDi817637.
KEGGiath:AT2G30870.

Organism-specific databases

TAIRiAT2G30870.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125746.
InParanoidiP42761.
KOiK00799.
OMAiFTEYLVG.
PhylomeDBiP42761.

Enzyme and pathway databases

BioCyciARA:AT2G30870-MONOMER.

Miscellaneous databases

PROiP42761.

Gene expression databases

GenevestigatoriP42761.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L."
    Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K.
    FEBS Lett. 335:189-192(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
    Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
    J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-7.
    Strain: cv. Landsberg erecta.
  6. "Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase."
    Bianchi M.W., Roux C., Vartanian N.
    Physiol. Plantarum 116:96-105(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  8. "Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death."
    Ryu H.Y., Kim S.Y., Park H.M., You J.Y., Kim B.H., Lee J.S., Nam K.H.
    Biochem. Biophys. Res. Commun. 379:417-422(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY GROWTH REGULATORS, DISRUPTION PHENOTYPE, INTERACTION WITH BAK1.

Entry informationi

Entry nameiGSTFA_ARATH
AccessioniPrimary (citable) accession number: P42761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.