Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P42761 (GSTFA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase F10

Short name=AtGSTF10
EC=2.5.1.18
Alternative name(s):
AtGSTF4
GST class-phi member 10
Protein EARLY RESPONSE TO DEHYDRATION 13
Gene names
Name:GSTF10
Synonyms:ERD13, GSTF4
Ordered Locus Names:At2g30870
ORF Names:F7F1.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.7

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Interacts with BAK1. Ref.8

Subcellular location

Cytoplasmcytosol Probable.

Tissue specificity

Expressed in roots, stems, floral buds, mature flowers and leaves. Ref.8

Induction

By dehydration stress, wounding, H2O2 and jasmonate, but not by growth regulators. Ref.6 Ref.8

Disruption phenotype

No visible phenotype, maybe due to the possible redundancy with GSTF9. Ref.8

Sequence similarities

Belongs to the GST superfamily. Phi family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 215214Glutathione S-transferase F10
PRO_0000185847

Regions

Domain2 – 8180GST N-terminal
Domain88 – 215128GST C-terminal
Region39 – 402Glutathione binding By similarity
Region52 – 532Glutathione binding By similarity
Region65 – 662Glutathione binding By similarity

Sequences

Sequence LengthMass (Da)Tools
P42761 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9A53C0AD19580C79

FASTA21524,230
        10         20         30         40         50         60 
MVLTIYAPLF ASSKRAVVTL VEKGVSFETV NVDLMKGEQR QPEYLAIQPF GKIPVLVDGD 

        70         80         90        100        110        120 
YKIFESRAIM RYIAEKYRSQ GPDLLGKTIE ERGQVEQWLD VEATSYHPPL LALTLNIVFA 

       130        140        150        160        170        180 
PLMGFPADEK VIKESEEKLA EVLDVYEAQL SKNEYLAGDF VSLADLAHLP FTEYLVGPIG 

       190        200        210 
KAHLIKDRKH VSAWWDKISS RAAWKEVSAK YSLPV 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L."
Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K.
FEBS Lett. 335:189-192(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-7.
Strain: cv. Landsberg erecta.
[6]"Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase."
Bianchi M.W., Roux C., Vartanian N.
Physiol. Plantarum 116:96-105(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[8]"Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death."
Ryu H.Y., Kim S.Y., Park H.M., You J.Y., Kim B.H., Lee J.S., Nam K.H.
Biochem. Biophys. Res. Commun. 379:417-422(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY GROWTH REGULATORS, DISRUPTION PHENOTYPE, INTERACTION WITH BAK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17673 mRNA. Translation: BAA04554.1.
AC004669 Genomic DNA. Translation: AAC20721.1.
CP002685 Genomic DNA. Translation: AEC08450.1.
AY128398 mRNA. Translation: AAM91601.1.
BT000077 mRNA. Translation: AAN15396.1.
PIRS39542.
RefSeqNP_180644.1. NM_128639.3.
UniGeneAt.58604.
At.71298.

3D structure databases

ProteinModelPortalP42761.
SMRP42761. Positions 8-214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP42761. 1 interaction.
MINTMINT-8068911.
STRING3702.AT2G30870.1-P.

2D gel databases

SWISS-2DPAGEP42761.

Proteomic databases

PaxDbP42761.
PRIDEP42761.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30870.1; AT2G30870.1; AT2G30870.
GeneID817637.
KEGGath:AT2G30870.

Organism-specific databases

TAIRAT2G30870.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125746.
InParanoidP42761.
KOK00799.
OMAAIRGWIS.
PhylomeDBP42761.
ProtClustDBPLN02395.

Enzyme and pathway databases

BioCycARA:AT2G30870-MONOMER.

Gene expression databases

ArrayExpressP42761.
GenevestigatorP42761.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTFA_ARATH
AccessionPrimary (citable) accession number: P42761
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names