P42760 (GSTF6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F6
Short name=AtGSTF6
EC=2.5.1.18

Alternative name(s):
AtGSTF3
GST class-phi member 6
Glutathione S-transferase 1
Short name=AtGST1
Protein EARLY RESPONSE TO DEHYDRATION 11

Gene names

Name:	GSTF6
Synonyms:	ERD11, GST1, GSTF3
Ordered Locus Names:	At1g02930
ORF Names:	F22D16.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in camalexin biosynthesis by probably catalyzing the conjugation of GSH with indole-3-acetonitrile (IAN). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.10
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Induction	By dehydration stress, salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, copper, metolachlor, and the pathogens P.syringae and Hyaloperonospora parasitica. Ref.7 Ref.8 Ref.9
Disruption phenotype	No visible phenotype under normal growth conditions, but reduced levels of camalexin production during infection by B.cinerea. Ref.10
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification Plant defense Stress response
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from expression pattern PubMed 17028151. Source: TAIR response to cadmium ion Inferred from expression pattern PubMed 16502469PubMed 17075075. Source: TAIR response to oxidative stress Inferred from direct assay PubMed 12492832. Source: TAIR response to salt stress Inferred from expression pattern PubMed 17916636. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW response to water deprivation Inferred from expression pattern Ref.1. Source: TAIR toxin catabolic process Traceable author statement Ref.7. Source: TAIR
Cellular_component	cell wall Inferred from direct assay PubMed 16287169. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR mitochondrion Inferred from direct assay PubMed 12492832PubMed 14671022. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	camalexin binding Inferred from direct assay PubMed 21631432. Source: TAIR cobalt ion binding Inferred from direct assay PubMed 20018591. Source: TAIR copper ion binding Inferred from direct assay PubMed 16526091. Source: TAIR glutathione binding Inferred from direct assay PubMed 15159623. Source: TAIR glutathione transferase activity Inferred from electronic annotation. Source: EC quercitrin binding Inferred from direct assay PubMed 21631432. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Glutathione S-transferase F6		PRO_0000185846
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	89 – 208	120	GST C-terminal
	Region	12 – 13	2	Glutathione binding By similarity
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Experimental info
	Sequence conflict	12	1	S → F in BAA04553. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42760 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 113CED008A11902F	FASTA	208	23,486
	10 20 30 40 50 60 MAGIKVFGHP ASTATRRVLI ALHEKNVDFE FVHVELKDGE HKKEPFILRN PFGKVPAFED 70 80 90 100 110 120 GDFKIFESRA ITQYIAHEFS DKGNNLLSTG KDMAIIAMGI EIESHEFDPV GSKLVWEQVL 130 140 150 160 170 180 KPLYGMTTDK TVVEEEEAKL AKVLDVYEHR LGESKYLASD HFTLVDLHTI PVIQYLLGTP 190 200 TKKLFDERPH VSAWVADITS RPSAQKVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L." Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 335:189-192(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Characterization of a glutathione S-transferase gene ATGST1 in Arabidopsis thaliana." Yang K.Y., Kim E.Y., Kim K.S., Choi S.N., Guh J.O., Kim K.C., Cho B.-H. Plant Cell Rep. 17:700-704(1999) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	Yu G.-L., Ausubel F.M. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, GENE FAMILY, NOMENCLATURE.
[8]	"The rapid induction of glutathione S-transferases AtGSTF2 and AtGSTF6 by avirulent Pseudomonas syringae is the result of combined salicylic acid and ethylene signaling." Lieberherr D., Wagner U., Dubuis P.H., Metraux J.P., Mauch F. Plant Cell Physiol. 44:750-757(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings." Smith A.P., DeRidder B.P., Guo W.J., Seeley E.H., Regnier F.E., Goldsbrough P.B. J. Biol. Chem. 279:26098-26104(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY COPPER.
[10]	"Glutathione-indole-3-acetonitrile is required for camalexin biosynthesis in Arabidopsis thaliana." Su T., Xu J., Li Y., Lei L., Zhao L., Yang H., Feng J., Liu G., Ren D. Plant Cell 23:364-380(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17672 mRNA. Translation: BAA04553.1. Y11727 Genomic DNA. Translation: CAA72413.1. L12057 Genomic RNA. No translation available. AC009525 Genomic DNA. Translation: AAF02873.1. CP002684 Genomic DNA. Translation: AEE27497.1. CP002684 Genomic DNA. Translation: AEE27498.1. AY050332 mRNA. Translation: AAK91349.1. AY097392 mRNA. Translation: AAM19908.1.
IPI	IPI00548409.
PIR	G86159. S39541.
RefSeq	NP_001184893.1. NM_001197964.1. NP_171792.1. NM_100174.2.
UniGene	At.20350. At.23846.
3D structure databases
ProteinModelPortal	P42760.
SMR	P42760. Positions 3-207.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42760. 2 interactions.
STRING	3702.AT1G02930.1-P.
2D gel databases
SWISS-2DPAGE	P42760.
Proteomic databases
PaxDb	P42760.
PRIDE	P42760.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02930.1; AT1G02930.1; AT1G02930. AT1G02930.2; AT1G02930.2; AT1G02930.
GeneID	839515.
KEGG	ath:AT1G02930.
Organism-specific databases
TAIR	At1g02930.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
InParanoid	P42760.
KO	K00799.
OMA	QYIAHEF.
PhylomeDB	P42760.
ProtClustDB	CLSN2679613.
Gene expression databases
Genevestigator	P42760.
GermOnline	AT1G02930. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF6_ARATH

Accession

Primary (citable) accession number: P42760

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program