P42750 (UBC6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 6
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 6
Ubiquitin-conjugating enzyme E2-21 kDa 3
Ubiquitin-protein ligase 6

Gene names

Name:	UBC6
Ordered Locus Names:	At2g46030
ORF Names:	T3F17.32

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.3
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in roots, petals, sepals and silique walls. Ref.2 Ref.3 Ref.8
Induction	By senescence, but not by heat shock. Ref.3 Ref.8
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence CAA50503.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.3. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein transferase activity Inferred from direct assay Ref.3. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Ubiquitin-conjugating enzyme E2 6		PRO_0000082584
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	2	1	A → S in AAA32901. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42750 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: D833BA90995EDEF2	FASTA	183	20,785
	10 20 30 40 50 60 MASPSKRREM DMMKLMMSDY KVDTVNDDLQ MFYVTFHGPT DSLYQGGVWK IKVELPEAYP 70 80 90 100 110 120 YKSPSVGFVN KIYHPNVDES SGAVCLDVIN QTWSPMFDLI NVFESFLPQL LLYPNPSDPF 130 140 150 160 170 180 NGEAASLLMR DRAAYELKVK EYCEKYAKPE EILSDDDDDD SMSEDGSDSD DDDDDEIVGK ADP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana." Sullivan M.L., Carpenter T.B., Vierstra R.D. Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Green leaf.
[2]	"Floral expression of a gene encoding an E2-related ubiquitin-conjugating protein from Arabidopsis thaliana." Watts F.Z., Butt N., Layfield P., Machuka J., Burke J.F., Moore A.L. Plant Mol. Biol. 26:445-451(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[3]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed." Thoma S., Sullivan M.L., Vierstra R.D. Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19355 Genomic DNA. Translation: AAA32901.1. X71381 Genomic DNA. Translation: CAA50503.1. Sequence problems. DQ027021 mRNA. Translation: AAY44847.1. AC005397 Genomic DNA. Translation: AAC62907.1. CP002685 Genomic DNA. Translation: AEC10634.1. BT005208 mRNA. Translation: AAO63272.1. AK228245 mRNA. Translation: BAF00193.1.
PIR	S43785. S52661.
RefSeq	NP_566062.1. NM_130166.3.
UniGene	At.48598. At.68190.
3D structure databases
ProteinModelPortal	P42750.
SMR	P42750. Positions 7-150.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	4546. 1 interaction.
STRING	3702.AT2G46030.1-P.
Proteomic databases
PaxDb	P42750.
PRIDE	P42750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G46030.1; AT2G46030.1; AT2G46030.
GeneID	819211.
KEGG	ath:AT2G46030.
Organism-specific databases
TAIR	AT2G46030.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233452.
InParanoid	P42750.
KO	K10576.
OMA	PNVDESS.
PhylomeDB	P42750.
Enzyme and pathway databases
BioCyc	ARA:AT2G46030-MONOMER.
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P42750.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. SSF54495. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC6_ARATH

Accession

Primary (citable) accession number: P42750
Secondary accession number(s): O49739, O82365, Q4TZ03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 20, 2002
Last modified:	May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program