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P42750

- UBC6_ARATH

UniProt

P42750 - UBC6_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 6

Gene

UBC6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G46030-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 6 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 6
Ubiquitin-conjugating enzyme E2-21 kDa 3
Ubiquitin-protein ligase 6
Gene namesi
Name:UBC6
Ordered Locus Names:At2g46030
ORF Names:T3F17.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G46030.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Ubiquitin-conjugating enzyme E2 6PRO_0000082584Add
BLAST

Proteomic databases

PaxDbiP42750.
PRIDEiP42750.

Expressioni

Tissue specificityi

Expressed in roots, petals, sepals and silique walls.3 Publications

Inductioni

By senescence, but not by heat shock.2 Publications

Gene expression databases

GenevestigatoriP42750.

Interactioni

Protein-protein interaction databases

BioGridi4546. 1 interaction.
STRINGi3702.AT2G46030.1-P.

Structurei

3D structure databases

ProteinModelPortaliP42750.
SMRiP42750. Positions 7-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233452.
InParanoidiP42750.
KOiK10576.
OMAiPNVDESS.
PhylomeDBiP42750.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42750-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPSKRREM DMMKLMMSDY KVDTVNDDLQ MFYVTFHGPT DSLYQGGVWK
60 70 80 90 100
IKVELPEAYP YKSPSVGFVN KIYHPNVDES SGAVCLDVIN QTWSPMFDLI
110 120 130 140 150
NVFESFLPQL LLYPNPSDPF NGEAASLLMR DRAAYELKVK EYCEKYAKPE
160 170 180
EILSDDDDDD SMSEDGSDSD DDDDDEIVGK ADP
Length:183
Mass (Da):20,785
Last modified:June 20, 2002 - v2
Checksum:iD833BA90995EDEF2
GO

Sequence cautioni

The sequence CAA50503.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → S in AAA32901. (PubMed:8155884)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19355 Genomic DNA. Translation: AAA32901.1.
X71381 Genomic DNA. Translation: CAA50503.1. Sequence problems.
DQ027021 mRNA. Translation: AAY44847.1.
AC005397 Genomic DNA. Translation: AAC62907.1.
CP002685 Genomic DNA. Translation: AEC10634.1.
BT005208 mRNA. Translation: AAO63272.1.
AK228245 mRNA. Translation: BAF00193.1.
PIRiS43785.
S52661.
RefSeqiNP_566062.1. NM_130166.3.
UniGeneiAt.48598.
At.68190.

Genome annotation databases

EnsemblPlantsiAT2G46030.1; AT2G46030.1; AT2G46030.
GeneIDi819211.
KEGGiath:AT2G46030.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19355 Genomic DNA. Translation: AAA32901.1 .
X71381 Genomic DNA. Translation: CAA50503.1 . Sequence problems.
DQ027021 mRNA. Translation: AAY44847.1 .
AC005397 Genomic DNA. Translation: AAC62907.1 .
CP002685 Genomic DNA. Translation: AEC10634.1 .
BT005208 mRNA. Translation: AAO63272.1 .
AK228245 mRNA. Translation: BAF00193.1 .
PIRi S43785.
S52661.
RefSeqi NP_566062.1. NM_130166.3.
UniGenei At.48598.
At.68190.

3D structure databases

ProteinModelPortali P42750.
SMRi P42750. Positions 7-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4546. 1 interaction.
STRINGi 3702.AT2G46030.1-P.

Proteomic databases

PaxDbi P42750.
PRIDEi P42750.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G46030.1 ; AT2G46030.1 ; AT2G46030 .
GeneIDi 819211.
KEGGi ath:AT2G46030.

Organism-specific databases

TAIRi AT2G46030.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233452.
InParanoidi P42750.
KOi K10576.
OMAi PNVDESS.
PhylomeDBi P42750.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT2G46030-MONOMER.

Gene expression databases

Genevestigatori P42750.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
    Sullivan M.L., Carpenter T.B., Vierstra R.D.
    Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Green leaf.
  2. "Floral expression of a gene encoding an E2-related ubiquitin-conjugating protein from Arabidopsis thaliana."
    Watts F.Z., Butt N., Layfield P., Machuka J., Burke J.F., Moore A.L.
    Plant Mol. Biol. 26:445-451(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
    Thoma S., Sullivan M.L., Vierstra R.D.
    Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiUBC6_ARATH
AccessioniPrimary (citable) accession number: P42750
Secondary accession number(s): O49739, O82365, Q4TZ03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2002
Last modified: October 1, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3