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P42750

- UBC6_ARATH

UniProt

P42750 - UBC6_ARATH

Protein

Ubiquitin-conjugating enzyme E2 6

Gene

UBC6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (20 Jun 2002)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G46030-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 6 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 6
    Ubiquitin-conjugating enzyme E2-21 kDa 3
    Ubiquitin-protein ligase 6
    Gene namesi
    Name:UBC6
    Ordered Locus Names:At2g46030
    ORF Names:T3F17.32
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G46030.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Ubiquitin-conjugating enzyme E2 6PRO_0000082584Add
    BLAST

    Proteomic databases

    PaxDbiP42750.
    PRIDEiP42750.

    Expressioni

    Tissue specificityi

    Expressed in roots, petals, sepals and silique walls.3 Publications

    Inductioni

    By senescence, but not by heat shock.2 Publications

    Gene expression databases

    GenevestigatoriP42750.

    Interactioni

    Protein-protein interaction databases

    BioGridi4546. 1 interaction.
    STRINGi3702.AT2G46030.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP42750.
    SMRiP42750. Positions 7-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233452.
    InParanoidiP42750.
    KOiK10576.
    OMAiPNVDESS.
    PhylomeDBiP42750.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42750-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPSKRREM DMMKLMMSDY KVDTVNDDLQ MFYVTFHGPT DSLYQGGVWK    50
    IKVELPEAYP YKSPSVGFVN KIYHPNVDES SGAVCLDVIN QTWSPMFDLI 100
    NVFESFLPQL LLYPNPSDPF NGEAASLLMR DRAAYELKVK EYCEKYAKPE 150
    EILSDDDDDD SMSEDGSDSD DDDDDEIVGK ADP 183
    Length:183
    Mass (Da):20,785
    Last modified:June 20, 2002 - v2
    Checksum:iD833BA90995EDEF2
    GO

    Sequence cautioni

    The sequence CAA50503.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21A → S in AAA32901. (PubMed:8155884)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19355 Genomic DNA. Translation: AAA32901.1.
    X71381 Genomic DNA. Translation: CAA50503.1. Sequence problems.
    DQ027021 mRNA. Translation: AAY44847.1.
    AC005397 Genomic DNA. Translation: AAC62907.1.
    CP002685 Genomic DNA. Translation: AEC10634.1.
    BT005208 mRNA. Translation: AAO63272.1.
    AK228245 mRNA. Translation: BAF00193.1.
    PIRiS43785.
    S52661.
    RefSeqiNP_566062.1. NM_130166.3.
    UniGeneiAt.48598.
    At.68190.

    Genome annotation databases

    EnsemblPlantsiAT2G46030.1; AT2G46030.1; AT2G46030.
    GeneIDi819211.
    KEGGiath:AT2G46030.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19355 Genomic DNA. Translation: AAA32901.1 .
    X71381 Genomic DNA. Translation: CAA50503.1 . Sequence problems.
    DQ027021 mRNA. Translation: AAY44847.1 .
    AC005397 Genomic DNA. Translation: AAC62907.1 .
    CP002685 Genomic DNA. Translation: AEC10634.1 .
    BT005208 mRNA. Translation: AAO63272.1 .
    AK228245 mRNA. Translation: BAF00193.1 .
    PIRi S43785.
    S52661.
    RefSeqi NP_566062.1. NM_130166.3.
    UniGenei At.48598.
    At.68190.

    3D structure databases

    ProteinModelPortali P42750.
    SMRi P42750. Positions 7-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4546. 1 interaction.
    STRINGi 3702.AT2G46030.1-P.

    Proteomic databases

    PaxDbi P42750.
    PRIDEi P42750.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G46030.1 ; AT2G46030.1 ; AT2G46030 .
    GeneIDi 819211.
    KEGGi ath:AT2G46030.

    Organism-specific databases

    TAIRi AT2G46030.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233452.
    InParanoidi P42750.
    KOi K10576.
    OMAi PNVDESS.
    PhylomeDBi P42750.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT2G46030-MONOMER.

    Gene expression databases

    Genevestigatori P42750.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
      Sullivan M.L., Carpenter T.B., Vierstra R.D.
      Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Green leaf.
    2. "Floral expression of a gene encoding an E2-related ubiquitin-conjugating protein from Arabidopsis thaliana."
      Watts F.Z., Butt N., Layfield P., Machuka J., Burke J.F., Moore A.L.
      Plant Mol. Biol. 26:445-451(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    8. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
      Thoma S., Sullivan M.L., Vierstra R.D.
      Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiUBC6_ARATH
    AccessioniPrimary (citable) accession number: P42750
    Secondary accession number(s): O49739, O82365, Q4TZ03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: June 20, 2002
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3