UBC6

Functionⁱ

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	85	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central
ubiquitin-protein transferase activity
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ubiquitin-dependent protein catabolic process
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 6 (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 6 Ubiquitin carrier protein 6 Ubiquitin-conjugating enzyme E2-21 kDa 3 Ubiquitin-protein ligase 6
Gene namesⁱ	Name:UBC6 Ordered Locus Names:At2g46030 ORF Names:T3F17.32
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 2

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT2G46030
TAIRⁱ	locus:2062979 AT2G46030

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082584	1 – 183	Ubiquitin-conjugating enzyme E2 6Add BLAST		183

Proteomic databases

PaxDbⁱ	P42750
PRIDEⁱ	P42750

Expressionⁱ

Tissue specificityⁱ

Expressed in roots, petals, sepals and silique walls.3 Publications

Inductionⁱ

By senescence, but not by heat shock.2 Publications

Gene expression databases

ExpressionAtlasⁱ	P42750 baseline and differential
Genevisibleⁱ	P42750 AT

Interactionⁱ

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4546, 1 interactor
STRINGⁱ	3702.AT2G46030.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P42750
SMRⁱ	P42750
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0416 Eukaryota ENOG410XRC5 LUCA
HOGENOMⁱ	HOG000233452
InParanoidⁱ	P42750
KEGG Orthology (KO) More...KOⁱ	K10576
OMAⁱ	FANTIYH
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360ND8
PhylomeDBⁱ	P42750

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P42750-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASPSKRREM DMMKLMMSDY KVDTVNDDLQ MFYVTFHGPT DSLYQGGVWK 
        60         70         80         90        100
IKVELPEAYP YKSPSVGFVN KIYHPNVDES SGAVCLDVIN QTWSPMFDLI 
       110        120        130        140        150
NVFESFLPQL LLYPNPSDPF NGEAASLLMR DRAAYELKVK EYCEKYAKPE 
       160        170        180 
EILSDDDDDD SMSEDGSDSD DDDDDEIVGK ADP

Length:183

Mass (Da):20,785

Last modified:June 20, 2002 - v2

Checksum:ⁱD833BA90995EDEF2

GO

Sequence cautionⁱ

The sequence CAA50503 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	2	A → S in AAA32901 (PubMed:8155884).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19355 Genomic DNA Translation: AAA32901.1 X71381 Genomic DNA Translation: CAA50503.1 Sequence problems. DQ027021 mRNA Translation: AAY44847.1 AC005397 Genomic DNA Translation: AAC62907.1 CP002685 Genomic DNA Translation: AEC10634.1 CP002685 Genomic DNA Translation: ANM62735.1 BT005208 mRNA Translation: AAO63272.1 AK228245 mRNA Translation: BAF00193.1
PIRⁱ	S43785 S52661
NCBI Reference Sequences More...RefSeqⁱ	NP_001318433.1, NM_001337170.1 NP_566062.1, NM_130166.4
UniGeneⁱ	At.48598 At.68190

Genome annotation databases

EnsemblPlantsⁱ	AT2G46030.1; AT2G46030.1; AT2G46030 AT2G46030.5; AT2G46030.5; AT2G46030
GeneIDⁱ	819211
Grameneⁱ	AT2G46030.1; AT2G46030.1; AT2G46030 AT2G46030.5; AT2G46030.5; AT2G46030
KEGGⁱ	ath:AT2G46030

Protein	Similar proteins		Species	Score	Length
P42750	Ubiquitin-conjugating enzyme 6		ARATH		255
UBC6		ARATH		256
UBC6		ARATH		184

Protein	Similar proteins		Species	Score	Length
P42750	Ubiquitin-conjugating enzyme 6		ARATH		255
Uncharacterized protein		MANES		187
Uncharacterized protein		JATCU		217
UPI000B793C50		HEVBR		199
Uncharacterized protein		RHIMU		187
+95

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19355 Genomic DNA Translation: AAA32901.1 X71381 Genomic DNA Translation: CAA50503.1 Sequence problems. DQ027021 mRNA Translation: AAY44847.1 AC005397 Genomic DNA Translation: AAC62907.1 CP002685 Genomic DNA Translation: AEC10634.1 CP002685 Genomic DNA Translation: ANM62735.1 BT005208 mRNA Translation: AAO63272.1 AK228245 mRNA Translation: BAF00193.1
PIRⁱ	S43785 S52661
NCBI Reference Sequences More...RefSeqⁱ	NP_001318433.1, NM_001337170.1 NP_566062.1, NM_130166.4
UniGeneⁱ	At.48598 At.68190

3D structure databases

ProteinModelPortalⁱ	P42750
SMRⁱ	P42750
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4546, 1 interactor
STRINGⁱ	3702.AT2G46030.1

Proteomic databases

PaxDbⁱ	P42750
PRIDEⁱ	P42750

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT2G46030.1; AT2G46030.1; AT2G46030 AT2G46030.5; AT2G46030.5; AT2G46030
GeneIDⁱ	819211
Grameneⁱ	AT2G46030.1; AT2G46030.1; AT2G46030 AT2G46030.5; AT2G46030.5; AT2G46030
KEGGⁱ	ath:AT2G46030

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT2G46030
TAIRⁱ	locus:2062979 AT2G46030

Phylogenomic databases

eggNOGⁱ	KOG0416 Eukaryota ENOG410XRC5 LUCA
HOGENOMⁱ	HOG000233452
InParanoidⁱ	P42750
KEGG Orthology (KO) More...KOⁱ	K10576
OMAⁱ	FANTIYH
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360ND8
PhylomeDBⁱ	P42750

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P42750

PROⁱ

PR:P42750

Gene expression databases

ExpressionAtlasⁱ	P42750 baseline and differential
Genevisibleⁱ	P42750 AT

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC6_ARATH
Accessionⁱ	P42750Primary (citable) accession number: P42750 Secondary accession number(s): O49739, O82365, Q4TZ03
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	June 20, 2002
	Last modified:	May 23, 2018
	This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P42750 (UBC6_ARATH)

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Ubiquitin-conjugating enzyme E2 6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ