UBC5

Functionⁱ

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	85	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central
ubiquitin-protein transferase activity
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ubiquitin-dependent protein catabolic process
Source: TAIR
Inferred from direct assayⁱ
- 16339806

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 5 (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme 5 Ubiquitin carrier protein 5 Ubiquitin-conjugating enzyme E2-21 kDa 2 Ubiquitin-protein ligase 5
Gene namesⁱ	Name:UBC5 Ordered Locus Names:At1g63800 ORF Names:F24D7.1, T12P18.18
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G63800
TAIRⁱ	locus:2026644 AT1G63800

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082583	1 – 185	Ubiquitin-conjugating enzyme E2 5Add BLAST		185

Proteomic databases

PaxDbⁱ	P42749
PRIDEⁱ	P42749

Expressionⁱ

Tissue specificityⁱ

Expressed in developing ovules, but not in vascular tissues.1 Publication

Inductionⁱ

Not induced by heat shock.1 Publication

Gene expression databases

ExpressionAtlasⁱ	P42749 baseline and differential
Genevisibleⁱ	P42749 AT

Interactionⁱ

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	27904, 1 interactor
IntActⁱ	P42749, 1 interactor
STRINGⁱ	3702.AT1G63800.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P42749
SMRⁱ	P42749
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0416 Eukaryota ENOG410XRC5 LUCA
HOGENOMⁱ	HOG000233452
InParanoidⁱ	P42749
KEGG Orthology (KO) More...KOⁱ	K10576
OMAⁱ	RREMDYM
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360ND8
PhylomeDBⁱ	P42749

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P42749-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSPSKRREM DLMKLMMSDY KVEMINDGMQ EFFVEFSGPK DSIYEGGVWK 
        60         70         80         90        100
IRVELPDAYP YKSPSVGFIT KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV 
       110        120        130        140        150
NVFETFLPQL LLYPNPSDPL NGEAAALMMR DRPTYEQRVK EYCEKYAKPR 
       160        170        180 
ADTEEMSSDD EMSEDEYASD GDDEDDVAIA GKLDP

Length:185

Mass (Da):21,179

Last modified:June 20, 2002 - v2

Checksum:ⁱ76AE2D7B0C34F5D8

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	178	A → R in AAA32902 (PubMed:8155884).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19356 Genomic DNA Translation: AAA32902.1 DQ027020 mRNA Translation: AAY44846.1 AC010852 Genomic DNA Translation: AAG52444.1 AC011622 Genomic DNA Translation: AAG52422.1 CP002684 Genomic DNA Translation: AEE34149.1 AY074347 mRNA Translation: AAL67043.1 AY114061 mRNA Translation: AAM45109.1 AK230439 mRNA Translation: BAF02237.1
PIRⁱ	A96663 S43786
NCBI Reference Sequences More...RefSeqⁱ	NP_001321725.1, NM_001334127.1 NP_564817.2, NM_105055.3
UniGeneⁱ	At.43954 At.67556

Genome annotation databases

EnsemblPlantsⁱ	AT1G63800.1; AT1G63800.1; AT1G63800
GeneIDⁱ	842683
Grameneⁱ	AT1G63800.1; AT1G63800.1; AT1G63800
KEGGⁱ	ath:AT1G63800

Protein	Similar proteins		Species	Score	Length
P42749	UBC5		ARATH		185
Ubiquitin-conjugating enzyme 5		ARATH		170

Protein	Similar proteins		Species	Score	Length
P42749	UBC5		ARATH		185
Uncharacterized protein (Fragment)		Capsella rubella		214
UPI00053BAF45		CAMSA		186
Ubiquitin-conjugating enzyme 37		ARALL		185
Uncharacterized protein		BRARP		184
+4

Protein	Similar proteins		Species	Score	Length
P42749	UBC5		ARATH		185
Uncharacterized protein (Fragment)		Capsella rubella		214
UPI00053A9557		CAMSA		186
Ubiquitin-conjugating enzyme 37		ARALL		185
Uncharacterized protein		BRARP		184
+215

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19356 Genomic DNA Translation: AAA32902.1 DQ027020 mRNA Translation: AAY44846.1 AC010852 Genomic DNA Translation: AAG52444.1 AC011622 Genomic DNA Translation: AAG52422.1 CP002684 Genomic DNA Translation: AEE34149.1 AY074347 mRNA Translation: AAL67043.1 AY114061 mRNA Translation: AAM45109.1 AK230439 mRNA Translation: BAF02237.1
PIRⁱ	A96663 S43786
NCBI Reference Sequences More...RefSeqⁱ	NP_001321725.1, NM_001334127.1 NP_564817.2, NM_105055.3
UniGeneⁱ	At.43954 At.67556

3D structure databases

ProteinModelPortalⁱ	P42749
SMRⁱ	P42749
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	27904, 1 interactor
IntActⁱ	P42749, 1 interactor
STRINGⁱ	3702.AT1G63800.1

Proteomic databases

PaxDbⁱ	P42749
PRIDEⁱ	P42749

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G63800.1; AT1G63800.1; AT1G63800
GeneIDⁱ	842683
Grameneⁱ	AT1G63800.1; AT1G63800.1; AT1G63800
KEGGⁱ	ath:AT1G63800

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G63800
TAIRⁱ	locus:2026644 AT1G63800

Phylogenomic databases

eggNOGⁱ	KOG0416 Eukaryota ENOG410XRC5 LUCA
HOGENOMⁱ	HOG000233452
InParanoidⁱ	P42749
KEGG Orthology (KO) More...KOⁱ	K10576
OMAⁱ	RREMDYM
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360ND8
PhylomeDBⁱ	P42749

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P42749

PROⁱ

PR:P42749

Gene expression databases

ExpressionAtlasⁱ	P42749 baseline and differential
Genevisibleⁱ	P42749 AT

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBC5_ARATH
Accessionⁱ	P42749Primary (citable) accession number: P42749 Secondary accession number(s): Q4TZ04 , Q8VXY5, Q9CAE2, Q9CAJ4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	June 20, 2002
	Last modified:	May 23, 2018
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - P42749 (UBC5_ARATH)

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Ubiquitin-conjugating enzyme E2 5

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ