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Protein

Ubiquitin-conjugating enzyme E2 5

Gene

UBC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 5 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 5
Ubiquitin carrier protein 5
Ubiquitin-conjugating enzyme E2-21 kDa 2
Ubiquitin-protein ligase 5
Gene namesi
Name:UBC5
Ordered Locus Names:At1g63800
ORF Names:F24D7.1, T12P18.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G63800
TAIRilocus:2026644 AT1G63800

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825831 – 185Ubiquitin-conjugating enzyme E2 5Add BLAST185

Proteomic databases

PaxDbiP42749
PRIDEiP42749

Expressioni

Tissue specificityi

Expressed in developing ovules, but not in vascular tissues.1 Publication

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

ExpressionAtlasiP42749 baseline and differential
GenevisibleiP42749 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi27904, 1 interactor
IntActiP42749, 1 interactor
STRINGi3702.AT1G63800.1

Structurei

3D structure databases

ProteinModelPortaliP42749
SMRiP42749
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0416 Eukaryota
ENOG410XRC5 LUCA
HOGENOMiHOG000233452
InParanoidiP42749
KOiK10576
OMAiRREMDYM
OrthoDBiEOG09360ND8
PhylomeDBiP42749

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P42749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPSKRREM DLMKLMMSDY KVEMINDGMQ EFFVEFSGPK DSIYEGGVWK
60 70 80 90 100
IRVELPDAYP YKSPSVGFIT KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV
110 120 130 140 150
NVFETFLPQL LLYPNPSDPL NGEAAALMMR DRPTYEQRVK EYCEKYAKPR
160 170 180
ADTEEMSSDD EMSEDEYASD GDDEDDVAIA GKLDP
Length:185
Mass (Da):21,179
Last modified:June 20, 2002 - v2
Checksum:i76AE2D7B0C34F5D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178A → R in AAA32902 (PubMed:8155884).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19356 Genomic DNA Translation: AAA32902.1
DQ027020 mRNA Translation: AAY44846.1
AC010852 Genomic DNA Translation: AAG52444.1
AC011622 Genomic DNA Translation: AAG52422.1
CP002684 Genomic DNA Translation: AEE34149.1
AY074347 mRNA Translation: AAL67043.1
AY114061 mRNA Translation: AAM45109.1
AK230439 mRNA Translation: BAF02237.1
PIRiA96663
S43786
RefSeqiNP_001321725.1, NM_001334127.1
NP_564817.2, NM_105055.3
UniGeneiAt.43954
At.67556

Genome annotation databases

EnsemblPlantsiAT1G63800.1; AT1G63800.1; AT1G63800
GeneIDi842683
GrameneiAT1G63800.1; AT1G63800.1; AT1G63800
KEGGiath:AT1G63800

Similar proteinsi

Entry informationi

Entry nameiUBC5_ARATH
AccessioniPrimary (citable) accession number: P42749
Secondary accession number(s): Q4TZ04
, Q8VXY5, Q9CAE2, Q9CAJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2002
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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