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P42748 (UBC4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 4

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 4
Ubiquitin-conjugating enzyme E2-21 kDa 1
Ubiquitin-protein ligase 4
Gene names
Name:UBC4
Ordered Locus Names:At5g41340
ORF Names:MYC6.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.2

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Tissue specificity

Expressed in antipodal cells of developing seeds and in the vascular junction between the cotyledons or leaves and the hypocotyl. Not expressed in pollen. Ref.6

Induction

Not induced by heat shock. Ref.6

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from direct assay Ref.2. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from direct assay Ref.2. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 187187Ubiquitin-conjugating enzyme E2 4
PRO_0000082582

Sites

Active site851Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict1321R → H in AAA32900. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42748 [UniParc].

Last modified June 20, 2002. Version 2.
Checksum: B9588F0AE7C0F20A

FASTA18721,298
        10         20         30         40         50         60 
MSSPSKRREM DMMKLMMSDY KVETINDGMQ EFYVEFNGPK DSLYQGGVWK IRVELPDAYP 

        70         80         90        100        110        120 
YKSPSVGFIT KIYHPNVDEL SGSVCLDVIN QTWSPMFDLV NVFETFLPQL LLYPNPSDPL 

       130        140        150        160        170        180 
NGEAAALMMR DRPAYEQRVK EYCEKYAKPG EGSEDKSSDE ELSEEEYGSD NEDDDDDDVA 


IAGKPDP 

« Hide

References

« Hide 'large scale' references
[1]"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
Sullivan M.L., Carpenter T.B., Vierstra R.D.
Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
Tissue: Green leaf.
[2]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
Thoma S., Sullivan M.L., Vierstra R.D.
Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19354 Genomic DNA. Translation: AAA32900.1.
DQ027019 mRNA. Translation: AAY44845.1.
AB006707 Genomic DNA. Translation: BAB08506.1.
CP002688 Genomic DNA. Translation: AED94667.1.
AY096587 mRNA. Translation: AAM20237.1.
AY065360 mRNA. Translation: AAL38801.1.
PIRS43784.
RefSeqNP_568589.1. NM_123499.4.
UniGeneAt.28656.

3D structure databases

ProteinModelPortalP42748.
SMRP42748. Positions 7-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19387. 2 interactions.
IntActP42748. 1 interaction.

Proteomic databases

PaxDbP42748.
PRIDEP42748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G41340.1; AT5G41340.1; AT5G41340.
GeneID834136.
KEGGath:AT5G41340.

Organism-specific databases

TAIRAT5G41340.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233452.
InParanoidP42748.
KOK06689.
OMAYAKPGEG.
PhylomeDBP42748.

Enzyme and pathway databases

BioCycARA:AT5G41340-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP42748.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC4_ARATH
AccessionPrimary (citable) accession number: P42748
Secondary accession number(s): Q4TZ05, Q9FN66
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2002
Last modified: May 14, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names