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Protein

Ubiquitin-conjugating enzyme E2 4

Gene

UBC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 4
Ubiquitin carrier protein 4
Ubiquitin-conjugating enzyme E2-21 kDa 1
Ubiquitin-protein ligase 4
Gene namesi
Name:UBC4
Ordered Locus Names:At5g41340
ORF Names:MYC6.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G41340.
TAIRilocus:2177871. AT5G41340.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825821 – 187Ubiquitin-conjugating enzyme E2 4Add BLAST187

Proteomic databases

PaxDbiP42748.

Expressioni

Tissue specificityi

Expressed in antipodal cells of developing seeds and in the vascular junction between the cotyledons or leaves and the hypocotyl. Not expressed in pollen.1 Publication

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

GenevisibleiP42748. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi19387. 3 interactors.
IntActiP42748. 1 interactor.
STRINGi3702.AT5G41340.1.

Structurei

3D structure databases

ProteinModelPortaliP42748.
SMRiP42748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0416. Eukaryota.
ENOG410XRC5. LUCA.
HOGENOMiHOG000233452.
InParanoidiP42748.
OMAiRREMDYM.
OrthoDBiEOG09360ND8.
PhylomeDBiP42748.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P42748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPSKRREM DMMKLMMSDY KVETINDGMQ EFYVEFNGPK DSLYQGGVWK
60 70 80 90 100
IRVELPDAYP YKSPSVGFIT KIYHPNVDEL SGSVCLDVIN QTWSPMFDLV
110 120 130 140 150
NVFETFLPQL LLYPNPSDPL NGEAAALMMR DRPAYEQRVK EYCEKYAKPG
160 170 180
EGSEDKSSDE ELSEEEYGSD NEDDDDDDVA IAGKPDP
Length:187
Mass (Da):21,298
Last modified:June 20, 2002 - v2
Checksum:iB9588F0AE7C0F20A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132R → H in AAA32900 (PubMed:8155884).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19354 Genomic DNA. Translation: AAA32900.1.
DQ027019 mRNA. Translation: AAY44845.1.
AB006707 Genomic DNA. Translation: BAB08506.1.
CP002688 Genomic DNA. Translation: AED94667.1.
AY096587 mRNA. Translation: AAM20237.1.
AY065360 mRNA. Translation: AAL38801.1.
PIRiS43784.
RefSeqiNP_568589.1. NM_123499.5.
UniGeneiAt.28656.

Genome annotation databases

EnsemblPlantsiAT5G41340.1; AT5G41340.1; AT5G41340.
GeneIDi834136.
GrameneiAT5G41340.1; AT5G41340.1; AT5G41340.
KEGGiath:AT5G41340.

Similar proteinsi

Entry informationi

Entry nameiUBC4_ARATH
AccessioniPrimary (citable) accession number: P42748
Secondary accession number(s): Q4TZ05, Q9FN66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2002
Last modified: August 30, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families