P42748 (UBC4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 4
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 4
Ubiquitin-conjugating enzyme E2-21 kDa 1
Ubiquitin-protein ligase 4

Gene names

Name:	UBC4
Ordered Locus Names:	At5g41340
ORF Names:	MYC6.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.2
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in antipodal cells of developing seeds and in the vascular junction between the cotyledons or leaves and the hypocotyl. Not expressed in pollen. Ref.6
Induction	Not induced by heat shock. Ref.6
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.2. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Ubiquitin-conjugating enzyme E2 4		PRO_0000082582
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	132	1	R → H in AAA32900. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42748 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: B9588F0AE7C0F20A	FASTA	187	21,298
	10 20 30 40 50 60 MSSPSKRREM DMMKLMMSDY KVETINDGMQ EFYVEFNGPK DSLYQGGVWK IRVELPDAYP 70 80 90 100 110 120 YKSPSVGFIT KIYHPNVDEL SGSVCLDVIN QTWSPMFDLV NVFETFLPQL LLYPNPSDPL 130 140 150 160 170 180 NGEAAALMMR DRPAYEQRVK EYCEKYAKPG EGSEDKSSDE ELSEEEYGSD NEDDDDDDVA IAGKPDP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana." Sullivan M.L., Carpenter T.B., Vierstra R.D. Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Green leaf.
[2]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed." Thoma S., Sullivan M.L., Vierstra R.D. Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19354 Genomic DNA. Translation: AAA32900.1. DQ027019 mRNA. Translation: AAY44845.1. AB006707 Genomic DNA. Translation: BAB08506.1. CP002688 Genomic DNA. Translation: AED94667.1. AY096587 mRNA. Translation: AAM20237.1. AY065360 mRNA. Translation: AAL38801.1.
IPI	IPI00522441.
PIR	S43784.
RefSeq	NP_568589.1. NM_123499.4.
UniGene	At.28656.
3D structure databases
ProteinModelPortal	P42748.
SMR	P42748. Positions 7-147.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42748. 1 interaction.
Proteomic databases
PaxDb	P42748.
PRIDE	P42748.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G41340.1; AT5G41340.1; AT5G41340.
GeneID	834136.
KEGG	ath:AT5G41340.
Organism-specific databases
TAIR	At5g41340.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233452.
InParanoid	P42748.
KO	K06689.
OMA	DTDVIKL.
PhylomeDB	P42748.
ProtClustDB	CLSN2688956.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P42748.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC4_ARATH

Accession

Primary (citable) accession number: P42748
Secondary accession number(s): Q4TZ05, Q9FN66

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 20, 2002
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program