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P42748

- UBC4_ARATH

UniProt

P42748 - UBC4_ARATH

Protein

Ubiquitin-conjugating enzyme E2 4

Gene

UBC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (20 Jun 2002)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G41340-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 4 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 4
    Ubiquitin-conjugating enzyme E2-21 kDa 1
    Ubiquitin-protein ligase 4
    Gene namesi
    Name:UBC4
    Ordered Locus Names:At5g41340
    ORF Names:MYC6.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G41340.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 187187Ubiquitin-conjugating enzyme E2 4PRO_0000082582Add
    BLAST

    Proteomic databases

    PaxDbiP42748.
    PRIDEiP42748.

    Expressioni

    Tissue specificityi

    Expressed in antipodal cells of developing seeds and in the vascular junction between the cotyledons or leaves and the hypocotyl. Not expressed in pollen.1 Publication

    Inductioni

    Not induced by heat shock.1 Publication

    Gene expression databases

    GenevestigatoriP42748.

    Interactioni

    Protein-protein interaction databases

    BioGridi19387. 2 interactions.
    IntActiP42748. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP42748.
    SMRiP42748. Positions 7-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233452.
    InParanoidiP42748.
    KOiK06689.
    OMAiYAKPGEG.
    PhylomeDBiP42748.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42748-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSPSKRREM DMMKLMMSDY KVETINDGMQ EFYVEFNGPK DSLYQGGVWK    50
    IRVELPDAYP YKSPSVGFIT KIYHPNVDEL SGSVCLDVIN QTWSPMFDLV 100
    NVFETFLPQL LLYPNPSDPL NGEAAALMMR DRPAYEQRVK EYCEKYAKPG 150
    EGSEDKSSDE ELSEEEYGSD NEDDDDDDVA IAGKPDP 187
    Length:187
    Mass (Da):21,298
    Last modified:June 20, 2002 - v2
    Checksum:iB9588F0AE7C0F20A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti132 – 1321R → H in AAA32900. (PubMed:8155884)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19354 Genomic DNA. Translation: AAA32900.1.
    DQ027019 mRNA. Translation: AAY44845.1.
    AB006707 Genomic DNA. Translation: BAB08506.1.
    CP002688 Genomic DNA. Translation: AED94667.1.
    AY096587 mRNA. Translation: AAM20237.1.
    AY065360 mRNA. Translation: AAL38801.1.
    PIRiS43784.
    RefSeqiNP_568589.1. NM_123499.4.
    UniGeneiAt.28656.

    Genome annotation databases

    EnsemblPlantsiAT5G41340.1; AT5G41340.1; AT5G41340.
    GeneIDi834136.
    KEGGiath:AT5G41340.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19354 Genomic DNA. Translation: AAA32900.1 .
    DQ027019 mRNA. Translation: AAY44845.1 .
    AB006707 Genomic DNA. Translation: BAB08506.1 .
    CP002688 Genomic DNA. Translation: AED94667.1 .
    AY096587 mRNA. Translation: AAM20237.1 .
    AY065360 mRNA. Translation: AAL38801.1 .
    PIRi S43784.
    RefSeqi NP_568589.1. NM_123499.4.
    UniGenei At.28656.

    3D structure databases

    ProteinModelPortali P42748.
    SMRi P42748. Positions 7-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19387. 2 interactions.
    IntActi P42748. 1 interaction.

    Proteomic databases

    PaxDbi P42748.
    PRIDEi P42748.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G41340.1 ; AT5G41340.1 ; AT5G41340 .
    GeneIDi 834136.
    KEGGi ath:AT5G41340.

    Organism-specific databases

    TAIRi AT5G41340.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233452.
    InParanoidi P42748.
    KOi K06689.
    OMAi YAKPGEG.
    PhylomeDBi P42748.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G41340-MONOMER.

    Gene expression databases

    Genevestigatori P42748.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
      Sullivan M.L., Carpenter T.B., Vierstra R.D.
      Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Green leaf.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
      Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
      Thoma S., Sullivan M.L., Vierstra R.D.
      Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiUBC4_ARATH
    AccessioniPrimary (citable) accession number: P42748
    Secondary accession number(s): Q4TZ05, Q9FN66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: June 20, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3