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P42748

- UBC4_ARATH

UniProt

P42748 - UBC4_ARATH

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Protein
Ubiquitin-conjugating enzyme E2 4
Gene
UBC4, At5g41340, MYC6.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G41340-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 4
Ubiquitin-conjugating enzyme E2-21 kDa 1
Ubiquitin-protein ligase 4
Gene namesi
Name:UBC4
Ordered Locus Names:At5g41340
ORF Names:MYC6.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G41340.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Ubiquitin-conjugating enzyme E2 4
PRO_0000082582Add
BLAST

Proteomic databases

PaxDbiP42748.
PRIDEiP42748.

Expressioni

Tissue specificityi

Expressed in antipodal cells of developing seeds and in the vascular junction between the cotyledons or leaves and the hypocotyl. Not expressed in pollen.1 Publication

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

GenevestigatoriP42748.

Interactioni

Protein-protein interaction databases

BioGridi19387. 2 interactions.
IntActiP42748. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP42748.
SMRiP42748. Positions 7-147.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233452.
InParanoidiP42748.
KOiK06689.
OMAiYAKPGEG.
PhylomeDBiP42748.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42748-1 [UniParc]FASTAAdd to Basket

« Hide

MSSPSKRREM DMMKLMMSDY KVETINDGMQ EFYVEFNGPK DSLYQGGVWK    50
IRVELPDAYP YKSPSVGFIT KIYHPNVDEL SGSVCLDVIN QTWSPMFDLV 100
NVFETFLPQL LLYPNPSDPL NGEAAALMMR DRPAYEQRVK EYCEKYAKPG 150
EGSEDKSSDE ELSEEEYGSD NEDDDDDDVA IAGKPDP 187
Length:187
Mass (Da):21,298
Last modified:June 20, 2002 - v2
Checksum:iB9588F0AE7C0F20A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321R → H in AAA32900. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19354 Genomic DNA. Translation: AAA32900.1.
DQ027019 mRNA. Translation: AAY44845.1.
AB006707 Genomic DNA. Translation: BAB08506.1.
CP002688 Genomic DNA. Translation: AED94667.1.
AY096587 mRNA. Translation: AAM20237.1.
AY065360 mRNA. Translation: AAL38801.1.
PIRiS43784.
RefSeqiNP_568589.1. NM_123499.4.
UniGeneiAt.28656.

Genome annotation databases

EnsemblPlantsiAT5G41340.1; AT5G41340.1; AT5G41340.
GeneIDi834136.
KEGGiath:AT5G41340.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19354 Genomic DNA. Translation: AAA32900.1 .
DQ027019 mRNA. Translation: AAY44845.1 .
AB006707 Genomic DNA. Translation: BAB08506.1 .
CP002688 Genomic DNA. Translation: AED94667.1 .
AY096587 mRNA. Translation: AAM20237.1 .
AY065360 mRNA. Translation: AAL38801.1 .
PIRi S43784.
RefSeqi NP_568589.1. NM_123499.4.
UniGenei At.28656.

3D structure databases

ProteinModelPortali P42748.
SMRi P42748. Positions 7-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19387. 2 interactions.
IntActi P42748. 1 interaction.

Proteomic databases

PaxDbi P42748.
PRIDEi P42748.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G41340.1 ; AT5G41340.1 ; AT5G41340 .
GeneIDi 834136.
KEGGi ath:AT5G41340.

Organism-specific databases

TAIRi AT5G41340.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233452.
InParanoidi P42748.
KOi K06689.
OMAi YAKPGEG.
PhylomeDBi P42748.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G41340-MONOMER.

Gene expression databases

Genevestigatori P42748.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
    Sullivan M.L., Carpenter T.B., Vierstra R.D.
    Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Green leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
    Thoma S., Sullivan M.L., Vierstra R.D.
    Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiUBC4_ARATH
AccessioniPrimary (citable) accession number: P42748
Secondary accession number(s): Q4TZ05, Q9FN66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2002
Last modified: September 3, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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